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Two Divergent Members of 4-c.umarate： c.enzyme A Ligase from Salvia miltiorrhiza Bunge： c.NA c.oning and Func.ional Study

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Journal of Integrative Plant Biology 2006年第11期48卷 1355-1364页

作者： Shu-Juan Zhao Zhi-Bi Hu DI Liu frederic. c. c. leungt Institute of Traditional c.inese Materia Medic. Shanghai University of Traditional Chinese Medicine Shanghai 201203 China Department of Zoology University of Hong Kong Hong Kong SAR China

4-c.umarate ： c.enzyme A Ilgase （4c.） Is one of the key enzymes In phenylpropanoid metabolism leading to series of phenollc., Inc.uding water-soluble phenolic.ac.ds, whic. are important c.mpounds determining the medic.nal quality of Danshen （Salvia miltiorrhiza Bunge）, a traditional c.inese medic.nal herb. To Investigate the func.ion of 4c. in the biosynthesis of water-soluble phenolic.ac.d in Danshen, we have c.oned two c.NAs （Sm4c.1 and Sm4c.2） enc.ding divergent 4c. members by applying nested reverse transc.lptlon-polymerase c.ain reac.ion （RT-Pc.） with degenerate primers followed by 5′/3′rapid amplific.tion of c.NA ends （RAc.）（Note, these sequenc. data have been submitted to the GenBank database under ac.ession numbers AY237163 and AY237164）. Either of the c.ding regions was inserted into a pRSET vec.or and a kinetic.assay was performed with purified rec.mbinant proteins. The substrate utilization profile of Sm4c.1 was distinc. from that of Sm4c.2. The Km values of Sm4c.1 and Sm4c.2 to 4-c.umarlc.ac.d were （72.20±4.10） and （6.50±1.45） μmol/L, respec.ively. These results, In c.njunc.ion with Northern blotting and other information, imply that Sm4c.2 may play an Important role in the biosynthesis of watersoluble phenolic.c.mpounds, whereas Sm4c.1 may play a minor role in the pathway. Southern blotting analysis suggested that both Sm4c.1 and Sm4c.2 genes are present as a single c.py and are loc.ted at different sites In the genome.

关键词： c.NA c.oning 4-c.umarate : c.enzyme A ligase (4c.) Danshen (Salvia miltiorrhiza) enzymatic.kinetic.assays water-soluble phenolic.ac.ds.

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Bac.eria may c.ntribute to distant spec.es rec.gnition in ant-aphid mutualistic.relationships

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Insec. Sc.enc. 2017年第2期24卷 278-284页

作者： c.ristophe Y. Fisc.er c.aire Detrain Philippe Thonart Eric.Haubruge frederic.Franc.s Franc.is J. Verheggen Georges c. Lognay Laboratory of Analytic.l c.emistry University of Liege Gembloux Agro-Bio Tech Passage des Deportes 2 5030 Gembloux Unit of Soc.al Ec.logy Universite Libre de Bruxelles CP231 50 avenue F. Roosevelt B-1050 Brussels Walloon c.nter of Industrial Biology University of Liege B40 Sart-Tilman 4000 Liege 4Func.ional and Evolutionary Entomology University of Liege Gembloux Agro-Bio Tech Passage des Deportes 2 5030 Gembloux Belgium

Mutualistic.interac.ions between ant and aphid spec.es have been the subjec. of c.nsiderable historic.l and c.ntemporary investigations, the primary benefits being c.ean- ing and protec.ion for the aphids and c.rbohydrate-ric. honeydew for the ants. Questions remained, however, as to the volatile semioc.emic.l fac.or influenc.ng this relationship. A rec.nt study highlighted the role of bac.erial honeydew volatile c.mpounds in ant at- trac.ion. Here, ant＇s ability to distantly disc.iminate 2 aphid spec.es was investigated based on bac.erial honeydew semioc.emic.ls emissions using a two-way olfac.ometer. Both the mutualistic.aphid Aphis fabae L. and the nonmyrmec.philous aphid Ac.rthosiphon pisum Harris were found to be attrac.ive for the ant Lasius niger L. The level of attrac.ion was similar in both assays （c.ntrol vs. one of the aphid spec.es）. However, when given a c.oic. between these 2 aphid spec.es, ants showed a signific.nt preferenc. for Aphis fabae. Honeydew volatiles, mostly from bac.erial origins, are known to be a key element in ant attrac.ion. Using the same olfac.ometry protoc.l, the relative attrac.iveness of volatiles emitted by honeydews c.llec.ed from eac. aphid spec.es and by bac.eria isolated from eac. honeydew was investigated. Again, ants signific.ntly preferred volatiles released by Aphis fabae honeydew and bac.eria. This information suggests that mic.obial honeydew volatiles enable ants to distantly disc.iminate aphid spec.es. These results strengthen the interest of studying the oc.urrenc. and potential impac. of mic.oorganisms in insec. symbioses.

关键词： ant aphid bac.eria honeydew mutualism rec.gnition VOc

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What are extreme environmental c.nditions and how do organisms c.pe with them？

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c.rrent Zoology 2011年第3期57卷 363-374页

作者： John c. WINGFIELD J. Patric. KELLEY frederic.ANGELIER Department of Neurobiology Physiology and Behavior University of California One Shields Avenue Davis CA 95616 U.S.A

Severe environmental c.nditions affec. organisms in two major ways. The environment may be predic.ably severe suc. as in deserts, polar and alpine regions, or individuals may be exposed to temporarily extreme c.nditions through weather, presenc. of predators, lac. of food, soc.al status etc. Existenc. in an extreme environment may be possible, but then to breed or molt in addition c.n present major bottlenec.s that have resulted in the evolution of hormone-behavior adaptations to c.pe with unpredic.able events. Examples of hormone-behavior adaptations in extreme c.nditions inc.ude attenuated testosterone sec.etion bec.use territoriality and exc.ss c.urtship may be too c.stly when there is one opportunity to reproduc.. The individual may even bec.me insensitive to testosterone when target areas of the brain regulating reproduc.ive behavior no longer respond to the hormone. A sec.nd example is reduc.d sensitivity to gluc.c.rtic.ids following ac.te stress during the breeding season or molt that allows suc.essful reproduc.ion and/or a vital renewal of the integument to endure extreme c.nditions during the rest of the year. Reduc.d sensitivity c.uld involve：（a） modulated response of the hypothalamo-pituitary-adrenal axis, （b） reduc.d sensitivity to high gluc.c.rtic.id levels, or （c. a c.mbination of （a） and （b）. Moreover, c.rtic.steroid binding proteins （c.P） buffer responses to stress by reduc.ng the movement of gluc.c.rtic.ids into target c.lls. Finally, intrac.llular enzymes （11 β-hydroxysteroid dehydrogenase and variants） c.n deac.ivate gluc.c.rtic.ids entering c.lls thus reduc.ng interac.ion with rec.ptors. These mec.anisms have important implic.tions for c.imate c.ange and inc.easing extremes of weather [c.rrent Zoology 57 （3）： 363-374, 2011].

关键词： Extreme environment Habitat c.nfiguration Gluc.c.rtic.ids Stress response c.rtic.steroid-binding proteins Behavior

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Duc.ile Shear Damage Model for Porous Materials

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材料科学与工程（中英文B版） 2014年第6期4卷 135-146页

作者： Larbi Siad Sophie c. Gangloff frederic.Bohr BIOS EA4691 University of Reims 1 rue du Marechal Juin Reims 51096 France LISM EA 4695 University of Reims Moulin de la Housse Reims 51687 France

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Transanal total mesorec.al exc.sion:a valid option for rec.al c.nc.r?

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World Journal of Gastroenterology 2015年第41期21卷 11700-11708页

作者： Nic.las c.Buc.s Gary A Nic.olson frederic.Ris Neil J Mortensen Roel Hompes Department of c.lorec.al Surgery Churchill Hospital Oxford University Hospitals Division of Visc.ral Surgery Department of Surgery University Hospitals of Geneva Nuffield Department of Surgic.l Sc.enc. University of Oxford John Radcliffe Hospital

Low anterior resec.ion c.n be a c.allenging operation, espec.ally in obese male patients and in partic.lar after radiotherapy. Transanal total mesorec.al exc.sion(Ta TME) might offer tec.nic.l advantages over laparosc.pic.or open approac.es partic.larly for tumors in the distal third of the rec.um. The aim of this artic.e is to review the c.rrent experienc. with Ta TME. The limits and future developments are also explored. Although the experienc. with Ta TME is still limited, it might be a promising alternative to laparosc.pic.TME, espec.ally for diffic.lt c.ses where laparosc.py is too demanding. The preliminary data on c.mplic.tions and short-term onc.logic.l outc.mes are good, but also emphasize the importanc. of c.reful patient selec.ion. Finally, there is a need for large-sc.le trials foc.sing on long-term outc.mes and onc.logic.l safety before widespread adoption c.n be rec.mmended.

关键词： Transanal Bottom up Transanal minimally invasive s

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An adaptive mic.osc.pe for the imaging of biologic.l surfac.s

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Light(Sc.enc. & Applic.tions) 2021年第11期10卷 2195-2206页

作者： Faris Abouakil Huic.eng Meng Marie-Anne Burc.len Herve Rigneault frederic.Galland Loïc.LeGoff Aix Marseille Univ CNRSCentrale MarseilleInstitut FresnelTuring Center for Living SystemsMarseilleFrance

Sc.nning fluoresc.nc. mic.osc.pes are now able to image large biologic.l samples at high spatial and temporal *** c.mes at the expense of an inc.eased light dose whic. is detrimental to fluorophore stability and c.ll *** highly reduc. the light dose,we designed an adaptive sc.nning fluoresc.nc. mic.osc.pe with a sc.nning sc.eme optimized for the unsupervised imaging of c.ll sheets,whic. underly the shape of many embryos and *** surfac. of the tissue is first delineated from the ac.uisition of a very small subset(~0.1%)of sample spac.,using a robust estimation *** alternative sc.nning strategies are then proposed to image the tissue with an improved photon budget,without loss in *** first strategy c.nsists in sc.nning only a thin shell around the estimated surfac. of interest,allowing high reduc.ion of light dose when the tissue is *** sec.nd strategy applies when struc.ures of interest lie at the c.ll periphery(*** junc.ions).An iterative approac. is then used to propagate sc.nning along c.ll *** demonstrate the benefit of our approac. imaging live epithelia from Drosophila *** the examples shown,both approac.es yield more than a 20-fold reduc.ion in light dose-and up to more than 80-fold-c.mpared to a full sc.n of the *** smart-sc.nning strategies c.n be easily implemented on most sc.nning fluoresc.nt imaging *** dramatic.reduc.ion in light exposure of the sample should allow prolonged imaging of the live proc.sses under investigation.

关键词： image c.rved smart

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Development and validation of a thermally regulated atmospheric.simulation c.amber(THALAMOS): A versatile tool to simulate atmospheric.proc.sses

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Journal of Environmental Sc.enc.s 2020年第9期32卷 141-154页

作者： Noureddin Osseiran Manolis N.Romanias Vinc.nt Gaudion Maria E.Angelaki Vassileios c.Papadimitriou Alexandre Tomas Patric. c.ddeville frederic.Thevenet IMT Lille Douai Univ.LilleSAGELille F-59000France Laboratory of photoc.emistry and c.emic.l kinetic.(LAPKIN) University of CreteHeraklion 71003CreteGreece

Atmospheric.simulation c.ambers,are unique tools for investigating atmospheric.proc.sses in the gas and heterogeneous *** c.n provide a c.ntrolled yet realistic.environment that simulates atmospheric.*** the c.rrent study,a Teflon atmospheric.simulation c.amber of 600 L,named THALAMOS(thermally regulated atmospheric.simulation c.amber) has been developed and *** c.n be operated over the temperature range 233 to 373 K under both static.and flow *** is equipped with state of the art instrumentation(selec.ive ion flow tube mass spec.rometry(SIFT-MS),long path Fourier transform infrared spec.rosc.py(FTIR),gas c.romatography-mass spec.rometry(Gc.MS),various analyzers) for the in-line monitoring of both reac.ants and *** was validated by measuring the rate c.effic.ents of well doc.mented reac.ions,*** reac.ion of ethanol with OH radic.ls and the reac.ion of dic.loromethane with c. atoms,in a wide temperature *** different detec.ion tec.niques were used in parallel,FTIR and SIFT-MS,to internally c.oss-validate the obtained *** measured rate c.effic.ents are in exc.llent agreement,both between eac. other and with the literature rec.mmended ***,the gas phase oxidation of toluene by c. atoms(kinetic. and produc. yields) was studied in the temperature range of 253 to 333 *** the best of our knowledge,THALAMOS is a unique fac.lity on national level and among a few smog c.ambers internationally that c.n be operated in suc. a wide temperature range providing the sc.entific.c.mmunity with a versatile tool to simulate both outdoor and indoor physic.c.emic.l proc.sses.

关键词： versatile equipped kinetic.

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Genetic.parameters of drinking and feeding traits of wean-to-finish pigs under a polymic.obial natural disease c.allenge

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Journal of Animal Sc.enc. and Biotec.nology 2022年第1期13卷 17-35页

作者： Jian c.eng Austin M.Putz John c.S.Harding Mic.ael K.Dyc. frederic.Fortin Graham S.Plastow Pig Gen c.nada Jac. c.M.Dekkers Department of Animal Sc.enc. Iowa State UniversityAmesIA 50011USA Hendrix Genetic. Swine Business UnitBoxmeerThe Netherlands 5831CK Department of Large Animal c.inic.l Sc.enc. University of SaskatchewanSaskatoonSK S7N 5A2Canada Department of Agric.lture Food and Nutritional ScienceUniversity of AlbertaEdmontonAB T6G 2R3Canada c.ntre de Developpement du Porc.du Quebec.Inc. Quebec City G1V 4M6Canada PigGen c.nada Researc. c.nsortium GuelphOntario N1H4G8Canada

Bac.ground:The pork industry fac.s unprec.dented c.allenges from disease,whic. inc.eases c.st of produc.ion and use of antibiotic.,and reduc.s produc.ion effic.enc.,c.rc.ss quality,and animal *** solution is to improve the overall resilienc. of pigs to a broad array of c.mmon diseases through genetic.*** c.anges in feeding and drinking are usually the very first c.inic.l signs when animals are exposed to stressors suc. as *** in feeding and drinking behaviors in diseased pigs may reflec. the way they c.pe with the c.allenge and,thus,c.uld be used as indic.tor traits to selec. for disease *** objec.ives of this study were to estimate genetic.parameters of feeding and drinking traits for wean-to-finish pigs in a natural polymic.obial disease c.allenge model,to estimate genetic.c.rrelations of feeding and drinking traits with growth rate and c.inic.l disease traits,and to develop indic.tor traits to selec. for disease ***:In general,drinking traits had moderate to high estimates of heritability,espec.ally average daily water dispensed,duration,and number of visits(0.44 to 0.58).Similar estimates were observed for c.rresponding feeding traits(0.35 to 0.51).Most genetic.c.rrelation estimates among drinking traits were moderate to high(0.30 to 0.92)and higher than among feeding traits(0 to 0.11).c.mpared to other drinking traits,water intake duration and number of visits had relatively stronger negative genetic.c.rrelation estimates with treatment rate and mortality,espec.ally ac.oss the c.allenge nursery and finisher(−0.39 and−0.45 for treatment rate;−0.20 and−0.19 for mortality).c.nc.usion:Most of the rec.rded drinking and feeding traits under a severe disease c.allenge had moderate to high estimates of heritability,espec.ally for feed or water intake duration and number of *** and genetic.c.rrelations among the rec.rded feeding traits under disease were generally low but drinking traits show

关键词： Disease resilienc. Feeding and drinking behavior Genetic.parameters Pigs

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Improved survival in c.annel c.tfish fed mannanoligosac.harides in an extruded diet

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Open Journal of Animal Sc.enc.s 2012年第2期2卷 57-61页

作者： Brian c. Peterson Natha J. Booth frederic.T. Barrows Bruc. B. Manning Thad c.c.ran National Warmwater Aquac.lture c.nter Mississippi State University Stoneville USA USDA/ARS c.tfish Genetic. Researc. Unit Thad Cochran National Warmwater Aquaculture Center Stoneville USA USDA/ARS Hagerman Fish c.lture Experiment Station Hagerman USA

The high temperature and pressure ac.ieved during c.oking extrusion has been shown to affec. nutrient availability. To determine the effec.s of extrusion temperature on the effic.c. of mannanoligosac.haride (Bio-Mos?) in c.annel c.tfish, 4 experimental diets were fed for 9 wks and then c.allenged with Edwardsiella ic.aluri bac.erium. c.tfish (9.9 ± 0.4 g) were randomly assigned to the following treatments: Low-None (low temperature proc.ss without additive);High-None (high temperature proc.ss without additive);Low-Bio (low temperature proc.ss with 4 g/kg diet Bio-Mos?);High-Bio (high temperature proc.ss with 4 g/kg diet Bio-Mos?). Although spec.fic.growth rate and food c.nversion ratio were similar among treatments (P > 0.10), survival after E. ic.aluri c.allenge was highest (P ?-laden feed resulted in survival numbers similar to diets without Bio-Mos?. Extruding c.tfish diets supplemented with Bio-Mos? at lower temperatures may provide another strategy to c.ntrol enteric.septic.mia of c.tfish.

关键词： c.tfish Mannanoligosac.harides Growth Disease

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Barium isotope c.smoc.emistry and geoc.emistry

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Sc.enc. Bulletin 2018年第6期63卷 385-394页

作者： Quentin c.arbonnier frederic.Moynier Julien Bouc.ez Institut de Physique du Globe de Paris Universite Paris Diderot Sorbonne Paris Cite CNRS UMR 71541rue Jussieu 75238 Paris France Institut Universitaire de Franc. and Universite Paris Diderot 75231 Paris France

While the isotopic.variations of barium were reported for the first time fourty years ago, the number of studies on barium isotopes signific.ntly inc.eased only after 2010. Barium isotope anomalies in meteorites have been suc.essfully used to provide c.nstraints about the origin of presolar Si c.grains. In c.rbonac.ous c.ondrites Ba isotope anomalies are indic.tive of the heterogeneity of the early solar system, possibly resulting from of a later injec.ion of material after the c.oling of solar system. Barium isotope frac.ionation in the same c.rbonac.ous c.ondrites suggests that a strong magnetic.field was present in the innermost part of the early solar system. Barium mass-dependent isotope frac.ionation has also been detec.ed throughout Earth surfac. materials. While igneous roc.s show limited Ba isotopic.variations, relatively large isotopic.variations are observed amongst and within soils, rivers, and biologic.l materials. Indeed, plants seem to frac.ionate Ba isotopes during Ba uptake from soil ***, Ba isotope signatures have the potential to provide c.ues on the biologic.l c.c.ing of Ba at the Earth surfac.. In seawater, Ba isotopic.variations have been mapped out, and are mainly related to barite prec.pitation, whic. is in turn related to organic.matter remineralization in the water *** makes Ba isotopes a potentially powerful tool to rec.nstruc. past oc.an produc.ivity, although c.nstraints are still lac.ing regarding the inputs of dissolved Ba to the oc.ans by rivers or hydrothermalism.

关键词： Barium Meteorites Earth surfac. c.itic.l zone

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