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骨髓增生异常综合征表皮生长因子受体的表达及其与细胞凋亡的关系

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中华血液学杂志 2003年第1期24卷 22-24页

作者：李晓浦权 Cassandra Beckham Joachim h.Deeg 上海市第六人民医院血液科 200233 Clinical Research Division Fred Hutchinson cancer reseach center

目的　探索骨髓增生异常综合征 (MDS)造血细胞中表皮生长因子受体 (EGFR)表达及其与细胞凋亡的关系。方法　取 18例MDS患者骨髓有核细胞经离心涂片机制片 ,用免疫酶标记方法(碱性磷酸酶抗碱性磷酸酶系统 ,APAAP)及TdT介导的dUTP缺口末... 详细信息

目的　探索骨髓增生异常综合征 (MDS)造血细胞中表皮生长因子受体 (EGFR)表达及其与细胞凋亡的关系。方法　取 18例MDS患者骨髓有核细胞经离心涂片机制片 ,用免疫酶标记方法(碱性磷酸酶抗碱性磷酸酶系统 ,APAAP)及TdT介导的dUTP缺口末端 (荧光素 )标记 (TUNEL)法先后在同一标本上检测EGFR表达和凋亡信号。 9名正常人骨髓作对照。另外 ,18例MDS中有 15例、9名正常人中有 6名经流式细胞仪分选CD3 4+细胞并制备涂片后按上述方法进行EGFR和凋亡检测 ,并分析两者间的关系。结果　①MDS骨髓细胞EGFR表达 [(38.6± 2 4.6 ) %]高于正常对照 [(18.1±14 0 ) %](P <0 .0 5 ) ;②MDS细胞凋亡主要发生在EGFR阴性细胞 (16 .1%) ,EGFR阳性细胞中凋亡细胞仅占 1.4%(P <0 .0 1) ,EGFR表达与细胞凋亡呈负相关 (r =- 0 .70 1;tr=3.6 0 ;P <0 .0 1) ;③CD3 4+细胞EGFR表达阳性率RAEB RAEB t CMML组高于RA RAS组 ,分别为 (2 0 .6± 2 7.9) %和 (8.9±11 8) %,但差异无显著性 ,细胞凋亡率RAEB RAEB t CMML组低于RA RAS组 ,分别为 (18.2± 12 5 ) %和 (4 5 .2± 2 0 .5 ) %(P <0 .0 5 )。结论　MDS造血细胞过度表达EGFR。EGFR表达增高可能预示MDS的恶性增殖倾向并通过某种途径抑制细胞凋亡。

关键词：表皮生长因子受体骨髓增生异常综合征细胞凋亡 MDS EGFR 相关性

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实验室走出大流感？

环球科学 2012年第7期 72-77页

作者：弗雷德·古特尔（fred Guterl）石小东（翻译）

研究人员通过操作基因，在实验室培育出厂新的h5N1禽流感病毒毒株。由于禽流感病毒很可能轻易地在人群中传播，因此这项研究的安全性，相关结果是否应该公开发表引起了巨大的争议。

关键词：禽流感病毒实验室研究人员安全性

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论香港地下空间开发的规划、立法与发展经验

隧道建设 2008年第3期28卷 294-297页

作者：杨文武吴浩然刘正光茂盛亚洲工程顾问有限公司广东深圳518001

海滨城市香港山多平地少,土地是极为稀少的资源,但山岭地貌和坚硬晶质火成岩岩体很适合开发地下洞室和隧道。20世纪70年代、80年代起,就开始考虑发展地下空间,以满足都市发展的需求。为此,政府开始有计划地进行工程可行性研究,并进行相... 详细信息

海滨城市香港山多平地少,土地是极为稀少的资源,但山岭地貌和坚硬晶质火成岩岩体很适合开发地下洞室和隧道。20世纪70年代、80年代起,就开始考虑发展地下空间,以满足都市发展的需求。为此,政府开始有计划地进行工程可行性研究,并进行相关的立法工作,为规范地下空间的开发,建立了一套完整的地下空间开发体系。结合工程实例,就香港地下空间开发的研究、规划、开发、管理和立法作简要介绍。

关键词：地下空间洞室隧道地下室地铁车站规划立法开发

Tolerability and first hints for potential efficacy of motor-cognitive training under inspiratory hypoxia in health and neuropsychiatric disorders:A translational viewpoint

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Neuroprotection 2024年第3期2卷 228-242页

作者： Svea-Solveig Mennen Maren Franta Martin Begemann Justus B.h.Wilke Roman Schroder Umer Javed Butt Jonathan-Alexis Cortés-Silva Umut Cakir Marie Gura Markus de Marees Vinicius Daguano Gastaldi Johannes Burtscher Julie Schanz Matthias Bohn Martin Burtscher Andreas Fischer Luise Poustka Peter hammermann Markus Stadler fred Luhder Manvendra Singh Klaus-Armin Nave Kamilla Woznica Miskowiak hannelore Ehrenreich Clinical Neuroscience Max Planck Institute for Multidisciplinary SciencesCity CampusGottingenGermany Georg-August-University GottingenGermany Department of Sports Medicine Ruhr UniversityBochumGermany Institute of Sports Science University of LausanneLausanneSwitzerland Department of Clinical Chemistry and Interdisciplinary UMG Laboratory University Medical Center GottingenGottingenGermany Clinical Pharmacy Georg-August-UniversityGottingenGermany Faculty of Sports Science University of InnsbruckInnsbruckAustria Department of Child and Adolescent Psychiatry&Psychotherapy Center for Psychosocial MedicineUniversity Hospital HeidelbergHeidelbergGermany hBL Investmentpartners Gmbh München-FrankfurtGermany Datico Sport&health Gmbh BurghausenGermany Institute for Neuroimmunology and Multiple Sclerosis Research University Medical Center GottingenGottingenGermany Department of Neurogenetics Max Planck Institute for Multidisciplinary SciencesCity CampusGottingenGermany frederiksberg hospital and Department of Psychology Neurocognition and Emotion in Affective Disorders(NEAD)CentreCopenhagen Affective Disorder Research CentrePsychiatric Centre CopenhagenUniversity of CopenhagenCopenhagenDenmark Experimental Medicine Department of Psychiatry and PsychotherapyCentral Institute of Mental HealthMedical Faculty MannheimHeidelberg UniversityMannheimGermany

hypoxia is more and more perceived as pivotal physiological driving force,allowing cells in the brain and elsewhere to acclimate to lowered oxygen(O_(2)),and abridged *** mediating transcription program is induced by inspiratory hypoxia but also by intensive motor-cognitive tasks,provoking a relative decrease in O_(2) in relation to the acutely augmented *** termed this fundamental,demand-dependent drop in O_(2) availability"functional hypoxia."Major players in the hypoxia response are hypoxia-inducible factors(hIFs)and associated *** are transcription factors,stabilized by low O_(2) accessibility,and control expression of a multitude of *** in oxygen,however,can also be sensed via other pathways,among them the thiol-oxidase(2-aminoethanethiol)*** the far-reaching biological response to hypoxia,hitherto mostly observed in rodents,we initiated a translational project,combining mild to moderate inspiratory with functional *** had identified this combination earlier to benefit motor-cognitive attainment in mice.A total of 20 subjects were included:13 healthy individuals and 7 patients with depression and/or autism spectrum ***,we show that motor-cognitive training under inspiratory hypoxia(12%O_(2))for 3.5 h daily over 3 weeks is optimally *** present first signals of beneficial effects on general well-being,cognitive performance,physical fitness and *** in serum increases under hypoxia and flow cytometry analysis of blood reveals several immune cell types to be mildly modulated by *** obtain reliable information regarding the"add-on"value of inspiratory on top of functional hypoxia,induced by motor-cognitive training,a single-blind study—with versus without inspiratory hypoxia—is essential and outlined here.

关键词： brain cognition erythropoietin functional hypoxia high-parameter flow cytometry immune cells oxygen saturation physical fitness plasticity

用光调制反射研究GaSb/AlSb多层量子势阱的带间跃迁

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镇江船舶学院学报 1989年第2期3卷 38-44页

作者： h.Shen Z.hang 冷静 F.h.Pollak L.L.Chang W.I.Wang L.Esaki 美国纽约城市大学布鲁克林学院物理系美国IBM公司

我们测量了两个GaSb/AlSb多层量子势阱在77K时的光调制反射谱。在Г点（GaSb）亚带间的能量,理论与实验符合得甚好,这种一致性可延伸到空穴态被束缚而电子态低于AlSb导带最小值X点的整个能量范围, 这就使得有可能确定导带的补偿参数0.85... 详细信息

我们测量了两个GaSb/AlSb多层量子势阱在77K时的光调制反射谱。在Г点（GaSb）亚带间的能量,理论与实验符合得甚好,这种一致性可延伸到空穴态被束缚而电子态低于AlSb导带最小值X点的整个能量范围, 这就使得有可能确定导带的补偿参数0.85±0.08（应变前）和应变的贡献,超过这个能量范围时,理论与实验的偏离则为Г-X混合态提供了证据。

关键词： GaSb/AlSb 量子势阱带间跃迁

磺酸基转移酶1A1基因启动子区SNPs位点单体型及其功能分析

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磺酸基转移酶1A1基因启动子区SNPs位点单体型及其功能分析

Novel fibrin-fibronectin matrix accelerates mice skin wound healing

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作者：林育纯陈慧峰方飞林丽娜陈雯凌文华 Baitang Ning fred F Kadlubar 林忠宁中山大学公共卫生学院 National Center for Toxicological Research Ark ansas USA

应用基因组 DNA PCR 扩增 SULTIAI 基因 ATG 上游5′-UTR 启动子区-1344nt 至+11nt 的目的片段,定向克隆至 pGL3basic 载体,测序确定-664nt 至-266nt 序列保守区的等位基因型 SNP 位点;选择在中国人群中 SNP 构成的6种最常见(频率均＞1...

应用基因组 DNA PCR 扩增 SULTIAI 基因 ATG 上游5′-UTR 启动子区-1344nt 至+11nt 的目的片段,定向克隆至 pGL3basic 载体,测序确定-664nt 至-266nt 序列保守区的等位基因型 SNP 位点;选择在中国人群中 SNP 构成的6种最常见(频率均＞10%,共包含了90．9%)的和4种少见(频率＜0．015%)的单体型,构建基因克隆(依据频率由高到低分别为 pGL3b-S1A1P-h1至-h10);与 pRL-TK 共转染 NCI60中推荐的人肿瘤细胞株(在本研究中以人结肠直肠癌 hCT116细胞株为例);进行 Dual-Glow 荧光素酶检测分析启动子功能活性。通过测序确定存在-624G→C、-396A→G、-358A→C、 -341C→G 和-294T→C 的 SNP 位点。以空载体为参照,9种单体型克隆功能活性检测的 RLU(n=6)分别为 h1(GGACC,

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Bioactive Materials 2020年第4期5卷 949-962页

作者： Carlos Poblete Jara Ou Wang Thais Paulino do Prado Ayman Ismail Frank Marco Fabian han Li Licio A.Velloso Mark A.Carlson William Burgess Yuguo Lei William h.Velander Eliana P.Araújo Nursing School University of CampinasCampinasSPBrazil Laboratory of Cell Signaling University of CampinasCampinasSPBrazil Department of Chemical and Biomolecular Engineering University of NebraskaLincolnNEUSA Biomedical Engineering Program University of NebraskaLincolnNEUSA School of Biological Science University of NebraskaLincolnNEUSA Department of Surgery University of Nebraska Medical Center and the VA Nebraska-Western Iowa Health Care SystemUSA Mary and Dick holland Regenerative Medicine Program University of Nebraska Medical CenterOmahaNEUSA fred&Pamela Buffett Cancer Center University of Nebraska Medical CenterOmahaNEUSA

Plasma fibrinogen(F1)and fibronectin(pFN)polymerize to form a fibrin clot that is both a hemostatic and provisional matrix for wound *** 90%of plasma F1 has a homodimeric pair ofγchains(γγF1),and 10%has a heterodimeric pair ofγand more acidicγ′chains(γγ′F1).We have synthesized a novel fibrin matrix exclusively from a 1:1(molar ratio)complex ofγγ′F1 and pFN in the presence of highly active thrombin and recombinant Factor XIII(rFXIIIa).In this matrix,the fibrin nanofibers were decorated with pFN nanoclusters(termedγγ′F1:pFN fibrin).In contrast,fibrin made from 1:1 mixture ofγγF1 and pFN formed a sporadic distribution of“pFN droplets”(termedγγF1+pFN fibrin).Theγγ′F1:pFN fibrin enhanced the adhesion of primary human umbilical vein endothelium cells(hUVECs)relative to theγγF1+FN *** dimensional(3D)culturing showed that theγγ′F1:pFN complex fibrin matrix enhanced the proliferation of both hUVECs and primary human *** in the 3Dγγ′F1:pFN fibrin exhibited a starkly enhanced vascular morphogenesis while an apoptotic growth profile was observed in theγγF1+pFN *** toγγF1+pFN fibrin,mouse dermal wounds that were sealed byγγ′F1:pFN fibrin exhibited accelerated and enhanced *** study suggests that a 3D pFN presentation on a fibrin matrix promotes wound healing.

关键词： Fibrin Fibronectin Nanoclusters Wound healing

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