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Development of an in Vitro Assay to Evaluate the Biological Impact of 5G Technology on Human Skin—Shield Effect of a Tannin-Rich Plant Extract

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Journal of Cosmetics, Dermatological Sciences and Applications 2022年第2期12卷 100-108页

作者： Katharina Kappler franziska wandrey Jean-Eric Branka Stéphane Poigny Fred Zülli Mibelle Group Biochemistry Mibelle AG Buchs Switzerland Ephyscience Nantes France

Background: The new 5G telecommunication technology has stirred concerns about potential negative effects on human health by radiofrequency electromagnetic fields. As to whether skin biology can be affected by 5G waves has remained an unsolved challenge despite recent studies dealing with this issue. In particular, a strategy for rational design of an assay allowing to 1) reproducibly evaluate and decipher the 5G effects on skin as well as 2) test the potential protective effects of cosmetic active ingredients, has yet to be found. Here we describe an in vitro model of human normal keratinocytes irradiated by 5G waves and show their impact on two biomarkers of inflammatory stress, i.e. interleukin-1β (IL-1β) and reactive oxygen species (ROS) production. In addition, the capacity of a tannin-rich plant extract to protect against 5G impact is evaluated. Materials and Methods: In the first series of experiments, monolayers of human normal keratinocytes were irradiated or not (control) by 5G waves (3.5 MHz) in an anechoic chamber and were incubated at 37˚C for 24 hours. At the end of the incubation period, extracellular IL-1β and intracellular ROS were quantified using specific ELISA and colorimetric assays, respectively. In the second series of experiments, the effect of an overnight pre-incubation with increasing concentrations of a tannin-rich plant extract was evaluated. Additionally, we studied in a prospective way the expression of a set of 88 genes selected for their relevance to keratinocyte homeostasis, in relation to the 5G challenge as well as the protective effect of a tannin-rich plant extract. Results: 5G waves significantly increased IL-1β production by 48.4% (p β and ROS production. Finally, the expression of 47 genes was modified by 5G waves and/or by the tannin-rich plant extract. Conclusion: This is to our knowledge the first evaluation of the impact of 5G technology on inflammatory biomarkers of human normal skin cells. Here we provide an innova

关键词： 5G waves Human Normal Keratinocytes Inflammation ROS Interleukin-1 mRNA

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