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Multiple Functions of Nuclear DNA Helicase Ⅱ (RNA Helicase A) in Nucleic Acid Metabolism

Acta Biochimica et Biophysica Sinica 2004年第3期36卷 177-183页

作者： frank grosse Department of Biochemistry Institute of Molecular Biotechnology POB 100 813 D-07708 Jena Germany

Nuclear DNA helicase II (NDH II), or RNA helicase A (RHA), was initially discovered in mammals by conventional protein purification methods. Molecular cloning identified apparent sequence ho- mologies between NDH II and a Drosophila protein named maleless (MLE), the latter being essential for the Drosophila X-chromosome dosage compensation. Increasing amounts of evidence suggest that NDH II is involved in multiple aspects of cellular and viral DNA and RNA metabolism. Moreover the functions of NDH II may have potential clinical implications related to viral infection, autoimmune diseases, or even tumorigenesis.

关键词： DNA and RNA helicase maleless (MLE) X-chromosome dosage compensation dsRBD RGG-box transcription RNA processing RNA transport actin nucleolus SLE (systemic lupus erythematosus) virus

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Construction,expression and binding specificity of bispecific CD3×VEGFR-2 and CD3×NCAM antibodies in the single chain and diabody format

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Advances in Bioscience and Biotechnology 2013年第5期4卷 654-664页

作者： Anke Kopacek Thomas Boldicke Sarah Lergenmüller frank Berthold Markus Jensen Peter P.Müller Ludger grosse-Hovest Helmholtz Center for Infection Research Department of Molecular BiotechnologyBraunschweigGermany Department of Pediatric Oncology and Hematology University of CologneUniversity Hospital CologneCologneGermany Institute for Cell Biology Department of ImmunologyEberhard Karls UniversityTübingenGermany

Bispecific antibodies are recombinant proteins with novel immunological properties and therapeutic potential. Recombinant protein quality and activity of several bispecific antibodies comprising different variable domain combinations with respect to the parental monospecific single chain fragments (scFv) were evaluated after expression in bacteria or mammalian cells. The parental scFv proteins humanized anti-NCAM scFv, murine anti-VEGFR-2 scFv, murine and humanized anti-CD3 scFv, respectively, could successfully be expressed in E. coli, whereas the murine anti-NCAM scFv version could not be reliably detected. Bispecific CD3 × VEGFR-2 and CD3 × NCAM anti-bodies were expressed in the bispecific single chain and the single chain diabody format. However, the diabody derived from the murine anti-NCAM scFv could not efficiently be expressed in E. coli or in mammalian cells. Significant binding of the CD3 × NCAM single chain diabody comprising the humanized version of anti-CD3 and humanized version of anti-NCAM was efficient to both antigens. Nevertheless, binding of the bispecific single chain version to the NCAM antigen was inefficient in comparison to CD3 binding. In conclusion, the data could indicate that the result of scFv expression in bacteria may be predictive for the chances of success for functional expression of more complex bispecific derivatives.

关键词： Recombinant Antibody Single Chain Diabody Bispecific Antibody Protein Expression

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