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The Stripe Rust Resistance Gene Yr10 Encodes an Evolutionary-Conserved and Unique CC-NBS-LRR Sequence in Wheat

Molecular Plant 2014年第12期7卷 1740-1755页

作者： Wei Liu Michele Frick Rene Huel Cory L. Nykiforuk Xiaomin Wang Denis A. Gaudet francois eudes Robert L. Conner Alan Kuzyk Zhensheng Kang Andre Laroche State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwest A&F University YanglingShaanxi 712100 RR. China Agriculture and Agri-Food Canada Lethbridge Research Centre 5403 1st Avenue South Lethbridge Alberta T1J 4B1 Canada Current address: The International Commission on Missing Persons Alipasina 45A Sarajevo 71000 Bosnia and Herzegovina Current address: Emergent BioSolutions 155 Innovation Drive Winnipeg MB R3T 5Y3 Canada Current address: Agriculture and Agri-Food Canada Morden Research Centre Unit 100-101 Route 100 Morden MB R6M 1Y5 Canada Retired from Agriculture and Agri-Food Canada

The first seedling or all-stage resistance （R） R gene against stripe rust isolated from Moro wheat （Triticum aes- tivum L.） using a map-based cloning approach was identified as Yr10. Clone 4B of this gene encodes a highly evolutionary- conserved and unique CC-NBS-LRR sequence. Clone 4E, a homolog of Yr10, but lacking transcription start site （TSS） and putative TATA-box and CAAT-box, is likely a non-expressed pseudogene. Clones 4B and 4E are 84% identical and divergent in the intron and the LRR domain. Gene silencing and transgenesis were used in conjunction with inoculation with differen- tially avirulent and virulent stripe rust strains to demonstrate Yr10 functionality. The Yr10 CC-NBS-LRR sequence is unique among known CC-NBS-LRR R genes in wheat but highly conserved homologs （E = 0.0） were identified in Aegilops tauschii and other monocots including Hordeum vulgare and Brachypodium distachyon. Related sequences were also identified in genomic databases of maize, rice, and in sorghum. This is the first report of a CC-NBS-LRR resistance gene in plants with limited homologies in its native host, but with numerous homologous R genes in related monocots that are either host or non-hosts for stripe rust. These results represent a unique example of gene evolution and dispersion across species.

关键词： seedling or all-stage stripe rust resistance gene functionality transgenesis gene silencing homolog sequences coiled-coil region nucleot de-b nding site leucine-rich repeat domain.

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A highly conserved and unique CC-NBS-LRR sequence in grasses encodes the Yr10 stripe rust resistance gene

A highly conserved and unique CC-NBS-LRR sequence in grasses...

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第十三届全国植物基因组学大会

作者： Michele Frick Rene Huel Cory L.Nykiforuk Denis A.Gaudet francois eudes Robert L.Conner Alan Kuzyk andre Laroche Agriculture and Agrifood Canada Lethbridge Research CentreAlberta OCanada Agriculture and Agrifood Canada Morden Research CentreMorden OCanada

Attempts to clone Yr10 by map-based cloning led to the identification of two linked genomic clones encoding highly conserved and unique CC-NBS-LRR sequences. Clone 4B Correspondings to an expressed single copy transcript whereas 4E is a *** between the sequences showed some divergence in the intron and LRR sequences and they appear to form a two-member gene family.

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