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Effect of Molasses Addition at Ensiling on Ruminal In Situ Dry Matter and Nutrient Degradation of Whole-Plant Soybean Silage Harvested at Different Phenological Stages

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Agricultural Sciences 2022年第2期13卷 268-281页

作者： Lucas Ghedin Ghizzi Matheus Rebouças Pupo Celso Heinzen Jr. Tiago Antônio Del Valle francisco palma rennó Luiz Felipe Ferraretto Department of Animal Nutrition and Animal Production University of Sao Paulo Pirassununga Brazil Department of Animal and Dairy Sciences University of Wisconsin Madison USA Federal University of Santa Maria Santa Maria Brazil

Whole-plant soybean ensiling has limitations and challenges that affect silage fermentation patterns and reduce ruminal nutrient degradation. Perhaps either the addition of molasses at ensiling or harvesting at different phenological stages has the potential to enhance whole-plant soybean silage (WSS) ruminal degradation. This experiment was a completely randomized design with a 3 × 2 factorial arrangement of treatments evaluating the effects of molasses (0 and 40 g/kg fresh forage) and phenological stage (R5: beginning seed, R6: full seed, and R7: beginning maturity) on ruminal in situ degradation. Molasses increased effective ruminal degradability (ERD) of dry matter (DM) regardless of the phenological stage and increased ERD of crude protein at R5 and R6. The addition of molasses at later phenological stages increased ERD of neutral detergent fiber (NDF). There was a molasses × phenological stage interaction effect with greater fractions A and B of NDF at R7 and R5, respectively, but lower undegraded NDF at R5 and R6 with molasses addition. Although the benefits of adding molasses were more pronounced in R5 and R6, adding molasses to whole-plant soybean at ensiling is recommended regardless of phenological stage. Increasing the proportion of digestible nutrients at harvesting either by harvesting at later phenological stages or the addition of molasses is a powerful strategy to manage ruminal degradation of DM and nutrients in WSS.

关键词： Additive In Situ Degradation Ruminal Degradation Parameters Legume Ensiling

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Evaluation of Pre-Cecal, Ileal Digestibility and Glycemic Response in Fistulated Horses Fed with Different Starch Sources

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Food and Nutrition Sciences 2015年第1期6卷 151-160页

作者： Alexandre Augustode Oliveira Gobesso Mariano Etchichury José Esler de Freitas Jr. francisco palma rennó Hugo Tosi Department of Nutrition and Animal Production Faculty of Veterinary Medicine School of Veterinary Medicine and Animal Science University of Sao Paulo Pirassununga Brazil Department of Animal Science Sao Paulo State University Jaboticabal Brazil

The objective of this study was to evaluate the coefficients of pre-cecal, ileal digestibility, and glycemic response in cannulated horses fed with different starch sources. Four male horses were used in a 4 × 4 Balanced Latin Square cannulated in the cecum, with 36 months of age and average weight of 350 kg. The horses were fed with hay and the following sources of starch in concentrates: 1) corn (C);2) oats (O);3) sorghum (S);and 4) corn/oats (CO). The experiment consisted of four periods of eighteen days each, being the first twelve days of adaptation and six days to collect samples. There was no difference in cecal and ileal digestibility of starch, CP, ADF, NDF and OM among diets evaluated. However, horses fed with oats diet had higher values of pre-cecal apparent digestibility of DM in relation at the horses fed to sorghum and corn/oats diets. The values of CF digestibility were higher for equines fed with oats and sorghum diet compared to corn/oats diet. The horses fed with corn/oats diet had lower plasma glucose concentrations 30 minutes before the supply and higher insulin concentrations 210 minutes after ingestion of the foods. The digestibility of nutrients in equine diets, in pre-cecal and pre-ileal evaluation, presents significant differences, depending on the starch source provided, oat, corn or sorghum. The plasma glucose concentrations are directly correlated with the digestibility of starch.

关键词： Horses Pre-Cecal Digestibility Fistula Ileum Glucose

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Protein glycation:a wolf in sweet sheep’s clothing behind neurodegeneration

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Neural Regeneration Research 2024年第5期19卷 975-976页

作者： Ana B.Uceda francisco Leal-Pérez Miquel Adrover Institut Universitari d’Investigacióen Ciències de la Salut(IUNICS) Institut d’InvestigacióSanitària Illes Balears(IdISBa) Departament de Química Universitat de les Illes BalearsCtraPalma de MallorcaSpain

At the beginning of the 16th century,Paracelsus coined the maxim:“the dose makes the poison”.This principle can be applied to all living organisms,including organs and *** brain and its glial and neuronal cells are ... 详细信息

关键词： organs sheep sweet

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Nitric oxide and hydrogen sulfide share regulatory functions in higher plant events

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BIOCELL 2022年第1期46卷 1-5页

作者： francisco J.CORPAS SALVADOR GONZÁLEZ-GORDO MARTA RODRÍGUEZ-RUIZ MARÍA A.MUÑOZ-VARGAS JOSÉM.palma Group of Antioxidants Free Radicals and Nitric Oxide in BiotechnologyFood and AgricultureDepartment of BiochemistryCell and Molecular Biology of PlantsEstación Experimental del Zaidín(Spanish National Research CouncilCSIC)Granada18008Spain

Nitric oxide(NO)and hydrogen sulfide(HS)are two molecules that share signaling properties in plant and animal cells NO and H2S originate two farmilies of de rived mol ecules designated reactive nitrogen and sulfur species(RNS and RSS,respectively).These molecules are responsible for certain protein regulatory processes through posttranslational modifications(PTMs),being the most remarkable S nitrosation and persufidation,which afect the thiol group of cysteine *** and H2S can also exert regulatory functions due to their interaction through the iron present in proteins that contain heme groups or iron-sulfur dlusters,as reported mainly in animal ***,the available information in plant cells is still very limited thus *** higher plants,NO and H2S are involved in a myriad of physiological events from seed germination to fruit ripening,but also the mec hanism of response to biotic and abiotic stress *** vie wpoint manuscript highlights the functional regulatory parllelism of these two molecules which also interact with the metabolism of reactive oxygen species(ROS)in plant cells.

关键词： Ascorbate peroxidase Hydrogensulfide Nitricoxide Persulfidation S-nitrosation Signaling

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Plant peroxisomes at the crossroad of NO and H2O2 metabolism

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Journal of Integrative Plant Biology 2019年第7期61卷 803-816页

作者： francisco J.Corpas Luis A.del Río José M.palma Group of Antioxidants Free Radicals and Nitric Oxide in Biotechnology Food and Agriculture Department of Biochemistry Cell and Molecular Biology of Plants Estación Experimental del Zaidín CSIC

Plant peroxisomes are subcellular compartments involved in many biochemical pathways during the life cycle of a plant but also in the mechanism of response against adverse environmental *** organelles have an active nitro-oxidative metabolism under physiological conditions but this could be exacerbated under stress ***,peroxisomes have the capacity to proliferateand also undergo biochemical adaptations depending on the surrounding cellular *** important characteristic of peroxisomes is that they have a dynamic metabolism of reactive nitrogen and oxygen species(RNS and ROS)which generates two key molecules,nitric oxide(NO)and hydrogen peroxide(H2O2).These molecules can exert signaling functions by means of post-translational modifications that affect the functionality of target molecules like proteins,peptides or fatty *** review provides an overview of the endogenous metabolism of ROS and RNS in peroxisomes with special emphasis on polyamine and uric acid metabolism as well as the possibility that these organelles could be a source of signal molecules involved in the functional interconnection with other subcellular compartments.

关键词： Plant peroxisomes NO H2O2 metabolism

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Hydrogen sulfide:A novel component in Arabidopsis peroxisomes which triggers catalase inhibition

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Journal of Integrative Plant Biology 2019年第7期61卷 871-883页

作者： francisco J.Corpas Juan B.Barroso Salvador Gonzalez-Gordo Maria A.Munoz-Vargas Jose M.palma Group of Antioxidants Free Radicals and Nitric Oxide in BiotechnologyFood and AgricultureDepartment of BiochemistryCell and Molecular Biology of PlantsEstacion Experimental del ZaidfnCSICC/Profesor Albareda 1E-18008 GranadaSpain Group of Biochemistry and Cell Signaling in Nitric oxide Department of Biochemistry and Molecular BiologyCampus"Las Lagunillas"E-23071University of JaenJaenSpain

Plant peroxisomes have the capacity to gen erate different reactive oxygen and nitrogen species(ROS and RNS),such as H2O2,superoxide radical(O2^·-),nitric oxide and peroxynitrite(ONOO^-).These organelles have an active nitrooxidative metabolism which can be exacerbated by adverse stress *** sulfide(H2S)is a new signaling gasotransmitter which can mediate the posttranslational modification(PTM)*** used Arabidopsis thaliana transgenic seedlings expressing cyan fluorescent protein(CFP)fused to a canonical peroxisome targeting signal 1(PTS1)to visualize peroxisomes in living cells,as well as a specific fluorescent probe which showed that peroxisomes contain H2S.H2S was also detected in chloroplasts under glyphosate-induced oxidative stress *** enzyme activities,including catalase,photorespiratory H2O2-generating glycolate oxidase(GOX)and hydroxypyruvate reductase(HPR),were assayed in vitro with a H2S *** line with the persulfidation of this enzyme,catalase activity declined significantly in the presence of the H2S *** corroborate the inhibitory effect of H2S on catalase activity,we also assayed pure catalase from bovine liver and pepper fruit-enriched samples,in which catalase activity was *** together,these data provide evidenee of the presence of H2S in plant peroxisomes which appears to regulate catalase activity and,consequently,the peroxisomal H2O2 metabolism.

关键词： Hydrogen sulfide triggers catalase inhibition

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Opposed elevational variation in prevalence and intensity of endoparasites and their vectors in a lizard

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Current Zoology 2018年第2期64卷 197-204页

作者： Lola ALVAREZ-RUIZ Rodrigo MEGIA-palma Senda REGUERA Santiago Rulz francisco J. ZAMORA-CAMACHO Jordi FIGUEROLA Gregorio MORENO-RUEDA Centro de Investigaciones sobre Desertificacion-CSIC Ctra. Naquera Kin. 4.5 Moncada Valencia E-46113 Spain Departarnento de Ecologia Evolutiva Museo Nacional de Ciencias Naturales-CSIC c/Jose Gutierrez Abascal Madrid E-28006 Spain Unit of Ethology and Animal Welfare Faculty of Veterinary University CEU Cardenal Herrera Alfara del Patriarca (Valencia) E-46113 Spain Servicio de Control de Mosquitos DiputaciGn Provincial de Huelva Centro de InvestigaciGn Biornedica en Red Epidemiologia y Salud Publica (CIBERESP) Huelva Spain CIBER de Epidemiologia y Salud Peblica (CIBERESP) Spain Departamento de Biogeografia y Carnbio Global Museo Nacional de Ciencias Naturales-CSIC C/Jose Gutierrez Abascal 2 Madrid E-28006 Spain Departarnento de Ecologia de Hurnedales Estacion Biologica de Dofiana-CSIC c/Americo Vespucio s/n Sevilla E-41092 Spain Departamento de Zoologia Facultad de Ciencias Universidad de Granada Granada E-18071 Spain

Studying the causes of parasite geographic distribution is relevant to understand ecological and evolutionary processes that affect host populations as well as for species conservation. Temperature is one of the most important environmental variables affecting parasite distribution, as raising temperatures positively affect development, reproduction, and rate of transmission of both endo- and ectoparasites. In this context, it is generally accepted that, in mountains, parasite abundance decreases with elevation. However, empirical evidence on this topic is limited. In the present study, we analyzed the elevational variation of hemoparasites and ectoparasites of a lizard, , along a 2,200-m elevational gradient in Sierra Nevada (SE Spain). As predicted, ectoparasite (mites, ticks, mosquitoes, and sandflies) abundance decreased with elevation. However, hemoparasite prevalence and intensity in the lizard augmented with altitude, showing a pattern contrary to their vectors (mites). We suggest that tolerance to hemoparasites may increase with elevation as a consequence of lizards at high altitudes taking advantage of increased body condition and food availability, and reduced oxidative stress. Moreover, lizards could have been selected for higher resistance against hemoparasites at lowlands (where higher rates of replication are expected), thus reducing hemoparasite prevalence and load. Our findings imply that, in a scenario of climate warming, populations of lizards at high elevation may face increased abundance of ectoparasites, accompanied with strong negative effects.

关键词：寄生虫蜥蜴向量寄生物环境变量实验证据可获得性身体状况

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Specific primers design based on the superoxide dismutase b gene for Trypanosoma cruzi as a screening tool:Validation method using strains from Colombia classified according to their discrete typing unit

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Asian Pacific Journal of Tropical Medicine 2014年第11期7卷 854-859页

作者： francisco Olmo Javier Escobedo-Ortegón Patricia palma Manuel Sánchez-Moreno Ana Mejía-Jaramillo Omar Triana Clotilde Marín Grupo de Parasitologia Molecular Departmento de Parasitologia Universidad de Granada Laboratorio de Zoonosis y ETV's C.I.R.Dr.Hideyo NoguchiUniversidad Autonoma de YucatanAve.Itzaes #490 x 5997000 MeridaYucatan(Mexico) Grupo Biologiay Control de Enfermedades Infecciosas BCEI-SIU Instituto de BiologiaUniversidad de Antioquia

Objective:To classify 21 new isolates of Trypanosoma cruai(***) according to the Discrete Typing Unit(DTU) which they belong to,as well as tune up a new pair of primers designed to detect the parasite in biological ***:Strains were isolated,DNA extracted,and classified by using three Polymerase Chain Reactions(PCR).Subsequently this DNA was used along with other isolates of various biological samples,for a new PCR using primers ***,the amplified fragments were ***:It was observed the predominance of DTU i in Colombia,as well as the specificity of our primers for detection of ***,while no band was obtained when other species were ***:This work reveals the genetic variability of 21 new isolates of *** in *** primers confirmed their specificity for detecting the presence of ***.

关键词： Trypanosoma cruzi Polymerase chain reaction Colombia Superoxide dismutase gene b-based primers Discrete typing unit

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Hyperglycemia and reduced adiposity of streptozotocin-induced diabetic mice are not alleviated by oral benzylamine supplementation

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World Journal of Diabetes 2022年第9期13卷 752-764页

作者： Christian Carpéné Kristiyan Stiliyanov Atanasov francisco Les Josep Mercader Barcelo Institut des Maladies Métaboliques et Cardiovasculaires INSERM UMR 1297Toulouse 31432France Molecular Biology and One Health research group Department of Fundamental Biology and Health SciencesUniversity of the Balearic IslandsPalma 07122Spain Department of Pharmacy Faculty of Health SciencesUniversidad San JorgeVillanueva de GállegoZaragoza 50830Spain

BACKGROUND Benzylamine(Bza)oral administration delays the onset of hyperglycemia in insulin-resistant db-/-mice;a genetic model of obesity and type 2 *** To extend the antihyperglycemic properties of oral benzylamine to a model of insulin-deficient type 1 *** Male Swiss mice were rendered diabetic by streptozotocin treatment(STZ)and divided in two groups:one received 0.5%Bza as drinking solution for 24 d(STZ Bza-drinking)while the other was drinking water ad *** groups were constituted in age-matched,nondiabetic *** intake,liquid intake,body weight gain and nonfasting blood glucose levels were followed during *** the end of treatment,fasted glycemia,liver and white adipose tissue(WAT)mass were measured,while glucose uptake assays were performed in *** STZ diabetic mice presented typical features of insulin-deficient diabetes:reduced body mass and increased blood glucose *** altered parameters were not normalized in the Bza-drinking group in spite of restored food and water *** consumption could not reverse the severe fat depot atrophy of STZ diabetic *** the nondiabetic mice,no difference was found between control and Bza-drinking mice for any *** isolated adipocytes,hexose uptake was partially activated by 0.1 mmol/L Bza in a manner that was obliterated in vitro by the amine oxidase inhibitor phenelzine and that remained unchanged after Bza *** of 0.1 mmol/L Bza in WAT was lower in STZ diabetic than in normoglycemic *** Bza supplementation could not normalize the altered glucose handling of STZ diabetic mice with severe WAT ***,its antidiabetic potential in obese and diabetic rodents does not apply to lipoatrophic type 1 diabetic mice.

关键词： Diabetes Adipocytes Amine oxidases Insulin-like agents Glucose transport Polydipsia

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Cardiometabolic risk through an integrative classification combining physical activity and sedentary behavior in European adolescents:HELENA study

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Journal of Sport and Health Science 2019年第1期8卷 55-62页

作者： Carlos Cristi-Montero palma Chillón Idoia Labayen José A.Casajus Marcela Gonzalez-Gross Jérémy Vanhelst Yannis Manios Luis A.Moreno francisco B.Ortega Jonatan R.Ruiz IRyS Group Physical Education School Pontificia Universidad Cato?lica de Valparai?o PROmoting FITness and Health through physical activity research group (PROFITH) Department of Physical Education and SportsFaculty of Sport Sciences University of Granada Department of Nutrition and Food Science University of the Basque Country Department of Health and Human Performance School of Health Sciences University of Zaragoza ImFine Research Group Facultad de Ciencias de la Actividad Física y del Deporte-INEF Universidad Politcnica de Madrid Lille Inflammation Research International Center University of Lille Department of Nutrition and Dietetics Harokopio University

Purpose: This study aims to compare adolescents' cardiometabolic risk score through an integrative classification of physical activity(PA),which involves the combination of moderate-to-vigorous physical activity(MVPA) and sedentary behavior(SB).Methods: A cross-sectional study derived from the Healthy Lifestyle in Europe by Nutrition in Adolescence Cross-Sectional Study database(2006-2008) was conducted in adolescents(n = 548; boys, 47.3%; 14.7 ± 1.2 years) from 10 European cities. MVPA and SB were objectively measured using accelerometry. Adolescents were divided into 4 categories according to MVPA(meeting or not meeting the international recommendations) and the median of SB time(above or below sex-and age-specific median) as follows: High-SB & Inactive, Low-SB & Inactive,High-SB & Active, and Low-SB & Active. A clustered cardiometabolic risk score was computed using the homeostatic model assessment, systolic blood pressure, triglycerides, total cholesterol/high-density lipoprotein cholesterol, sum 4 skinfolds, and cardiorespiratory fitness(CRF).Analyses of covariance were performed to discern differences on cardiometabolic risk scores among PA categories and each health ***: The cardiometabolic risk score was lower in adolescents meeting the MVPA recommendation and with less time spent in SB in comparison to the high-SB & Inactive group(p < 0.05). However, no difference in cardiometabolic risk score was established between High-SB or Low-SB groups in inactive adolescents. It is important to note that CRF was the only variable that showed a significant modification(higher)when children were compared from the category of physically inactive with "active" but not from high-to ***: Being physically active is the most significant and protective outcome in adolescents to reduce cardiometabolic risk. Lower SB does not exhibit a significant and extra beneficial difference.

关键词： Accelerometry Cardiovascular disease Exercise Metabolic disease Sedentary lifestyles

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