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Water Consumption and Vegetative Growth Progress in Resistant and Susceptible Olive Cultivars Infected by Verticillium dahliae

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Agricultural Sciences 2016年第4期7卷 230-238页

作者： Fahima Birem Esteban Alcántara-Vara francisco javier López-Escudero Departamento de Agronomía Universidad de Córdoba-Campus de Excelencia Internacional Agroalimentario cei A3 Edificio C4 Campus de Rabanales Córdoba Spain

Purpose: Water consumption and vegetative growth in resistant (“Frantoio”) and susceptible (“Picual”) olive cultivars infected with Verticillium dahliae were assessed. Methods: Plants were inoculated with the pathogen and disease evaluated in growth chamber. Mean daily water consumption and vegetative growth were recorded during 13 weeks. Results: Water consumption significantly decreased in inoculated plants compared to non-inoculated plants during the 3rd - 6th weeks after inoculation period. From the 9th week, a significantly lower consumption occurred in infected “Picual” plants compared with “Frantoio” plants. Vegetative growth was reduced in inoculated plants of both cultivars, although the reduction of the fresh weight was remarkably higher in “Picual” compared with controls due to defoliation and tissue desiccation, impeding the production of new shoots and leaves. Conversely, in “Frantoio”, defoliation was significantly reduced to low levels. Conclusions: Water consumption and vegetative growth in plants of resistant (“Frantoio”) and susceptible (“Picual”) olive cultivars inoculated by Verticillium dahliae were consistently associated with the progress of the wilt symptoms and the level of resistance of the cultivar. Growth and water consumption reductions in “Picual” were irreversible in almost all plants, while “Frantoio” plants were able to maintain water translocation, overcome the infection and avoid vascular occlusion, and restart vegetative growth.

关键词： Defoliating Pathotype Disease Recovery Olea Europaea Verticillium Wilt of Olive

Laparoscopic approach in gastrointestinal emergencies

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World Journal of Gastroenterology 2016年第9期22卷 2701-2710页

作者： Rosa M Jimenez Rodriguez Juan José Segura-Sampedro Mercedes Flores-Cortés francisco López-Bernal Cristobalina Martín Verónica Pino Diaz Felipe Pareja Ciuro javier Padillo Ruiz Department of Surgery Hospital Universitario Virgen del Rocio 41013 Sevilla Spain Hospital Universitario Son Espases 07010 Palma de Mallorca Spain

This review focuses on the laparoscopic approach to gastrointestinal emergencies and its more recent indications. Laparoscopic surgery has a specific place in elective procedures, but that does not apply in emergency situations. In specific emergencies, there is a huge range of indications and different techniques to apply, and not all of them are equally settle. We consider that the most controversial points in minimally invasive procedures are indications in emergency situations due to technical difficulties. Some pathologies, such as oesophageal emergencies, obstruction due to colon cancer, abdominal hernias or incarcerated postsurgical hernias, are nearly always resolved by conventional surgery, that is, an open approach due to limited intraabdominal cavity space or due to the vulnerability of the bowel. These technical problems have been solved in many diseases, such as for perforated peptic ulcer or acute appendectomy for which a laparoscopic approach has become a wellknown and globally supported procedure. On the other hand, endoscopic procedures have acquired further indications, relegating surgical solutions to a second place; this happens in cholangitis or pancreatic abscess drainage. This endoluminal approach avoids the need for laparoscopic development in these diseases. Nevertheless, new instruments and new technologies could extend the laparoscopic approach to a broader array of potentials procedures. There remains, however, a long way to go.

关键词： Minimally invasive surgery Gastrointestinal surgery Emergency surgery Digestive emergencies Abdominal emergencies

Alcoholic liver disease:Utility of animal models

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World Journal of Gastroenterology 2018年第45期24卷 5063-5075页

作者： Arantza Lamas-Paz Fengjie Hao Leonard J Nelson Maria Teresa Vázquez Santiago Canals Manuel Gómez del Moral Eduardo Martínez-Naves Yulia A Nevzorova francisco javier Cubero Department of Immunology Ophthalmology and ORLComplutense University School of MedicineMadrid 28040Spain 12 de Octubre Health Research Institute(imas12) Madrid 28041Spain Institute for Bioengineering(IBioE) School of EngineeringFaraday BuildingThe University of EdinburghEdinburgh EH9 3 JLScotlandUnited Kingdom Department of Human Anatomy and Embryology Complutense University School of MedicineMadrid 28040Spain Instituto de Neurociencias Consejo Superior de Investigaciones CientíficasUniversidad Miguel HernándezSan Juan de Alicante 03550Spain Department of Cell Biology Complutense University School of MedicineMadrid 28040Spain Department of Genetics Physiology and MicrobiologyFaculty of BiologyUniversidad ComplutenseMadrid 28040Spain Department of Internal Medicine Ⅲ University Hospital RWTH AachenAachen 52062Germany

Alcoholic liver disease(ALD) is a major cause of acute and chronic liver injury. Extensive evidence has been accumulated on the pathological process of ALD during the past decades. However, effective treatment options for ALD are very limited due to the lack of suitable in vivo models that recapitulate the full spectrum of ALD. Experimental animal models of ALD, particularly rodents, have been used extensively to mimic human ALD. An ideal animal model should recapitulate all aspects of the ALD process, including significant steatosis, hepatic neutrophil infiltration, and liver injury. A better strategy against ALD depends on clear diagnostic biomarkers, accurate predictor(s) of its progression and new therapeutic approaches to modulate stop or even reverse the disease. Numerous models employing rodent animals have been established in the last decades to investigate the effects of acute and chronic alcohol exposure on the initiation and progression of ALD. Although significant progress has been made in gaining better knowledge on the mechanisms and pathology of ALD, many features of ALD are unknown, and require further investigation, ideally with improved animal models that more effectively mimic human ALD. Although differences in the degree and stages of alcoholic liver injury inevitably exist between animal models and human ALD, the acquisition and translational relevance will be greatly enhanced with the development of new and improved animal models of ALD.

关键词： Steatohepatitis Cirrhosis Hepatocellular carcinoma Alcoholic liver disease Reactive oxygen species

Safety and Efficacy of Oral Mirodenafil in Mexican with Erectile Dysfunction

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International Journal of Clinical Medicine 2016年第9期7卷 628-638页

作者： Juan Gerardo Reyes-García Noemí Santos-Caballero francisco javier Flores-Murrieta Sección de Estudios de Posgrado e Investigación Escuela Superior de Medicina Instituto Politécnico Nacional Ciudad de México Mexico Landsteiner Scientific S.A. de C.V. Ciudad de México Mexico Unidad de Investigación en Farmacología Instituto Nacional de Enfermedades Respiratorias “Ismael Cosio Villegas” Ciudad de México Mexico

Erectile dysfunction is treated with 5-phospodiesterase inhibitors as Mirodenafil, which has shown its efficacy and safety in Koreans, however;no information in other populations is available. An open clinical trial study was designed to evaluate the efficacy and safety in real life of a fixed-dose of Mirodenafil in Mexican patients with erectile dysfunction. Forty-seven male patients received a 100 mg tablet of Mirodenafil, during 12 weeks. Primary outcome efficacy measure was the percentage of male patients with successful intercourse. Secondary outcomes measures included patient satisfaction, mood and self-esteem level. Safety assessments included laboratory tests, vital signs, physical examination, 12-lead electrocardiogram recordings, and incidence of adverse events by patients. Oral administration of Mirodenafil improved in an 80% - 90% the number of successful intercourses from 7 to 84 days of treatment. Moreover, patients reported a significant increment in their sexual satisfaction, mood and self-esteem. Mirodenafil treatment did not modify vital signs nor anthropometric parameters during 84 days. Mild headache was the most frequent adverse event (17.0%) and there were no severe adverse events during pharmacological treatment. Data suggest that oral Mirodenafil is safety, well tolerated and effective in the Mexican population with erectile dysfunction.

关键词： Efficacy Erectile Dysfunction Mexicans Mirodenafil Safety

Mediation role of cardiorespiratory fitness on the association between fatness and cardiometabolic risk in European adolescents: The HELENA study

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Journal of Sport and Health Science 2021年第3期10卷 360-367页

作者： Carlos Cristi-Montero javier Courel-Ibanez francisco B.Ortega Jose Castro-Pinero Alba Santaliestra-Pasias Angela Polito Jeremy Vanhelst Ascension Marcos Luis M.Moreno Jonatan R.Ruiz IRyS Group Physical Education SchoolPontificia Universidad Catolicade ValparaisoValparaisso 2530388Chile Department of Physical Activity and Sport Faculty of Sport SciencesUniversity of MurciaMurcia 30071Spain PROmoting FITness and Health through Physical Activity Research Group(PROFITH) Department of Physical Education and SportsFaculty of Sport SciencesUniversity of GranadaGranada 18001Spain Department of Physical Education Faculty of Education SciencesUniversity of CadizPuerto Real 11003Spain Department of Health and Human Performance School of Health SciencesUniversity of ZaragozaZaragoza 50001Spain Growth ExerciseNutrition and Development(GENUD)Research GroupZaragoza 50001Spain National Institute for Food and Nutrition Research Rome 80070Italy Lille Inflammation Research International Center University of LilleLille 59000France Immunonutrition Research Group Department Metabolism and NutritionInstitute of Food Science and Technology and Nutrition(ICTAN)Spanish National Research Council(CSIC)Madrid E-28040 Spain

Purpose:This study was aimed to analyze the mediation role of cardiorespiratory fitness(CRF)on the association between fatness and cardiometabolic risk scores(CMRs)in European ***:A cross-sectional study was conducted in adolescents(n=525;46%boys;14.1±1.1 years old,mean ±SD)from 10 European cities involved in the Healthy Lifestyle in Europe by Nutrition in Adolescence *** was measured by means of the shuttle run test,while fatness measures included body mass index(BMI),waist to height ratio,and fat mass index estimated from skinfold thicknesses.A clustered CMRs was computed by summing the standardized values of homeostasis model assessment,systolic blood pressure,triglycerides,total cholesterol/highdensity lipoprotein cholesterol ratio,and ***:Linear regression models indicated that CRF acted as an important and partial mediator in the association between fatness and CMRs in 1217-year-old adolescents(for BMI:coefficients of the indirect role b=0.058(95%confidence interval(95%CI):0.023-0.101),Sobel test z=3.11(10.0%mediation);for waist to height ratio:b=4.279(95%CI:2.242-7.059),z=3.86(11.5%mediation);and for fat mass index:b=0.060(95%CI:0.020-0.106),z=2.85(9.4%mediation);all p<0.01).Conclusion:In adolescents,the association between fatness and CMRs could be partially decreased with improvements to fitness levels;therefore,CRF contribution both in the clinical field and public health could be important to consider and promote in adolescents independently of their fatness levels.

关键词： Cardiovascular disease Children Fat mass Fitness Health Physical activity

Identification and heterologous expression of the globomycin biosynthetic gene cluster

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Synthetic and Systems Biotechnology 2023年第2期8卷 206-212页

作者： Daniel Oves-Costales Tetiana Gren Eva Baggesgaard Sterndorff Jesús Martín francisco javier Ortiz-Lopez Tue S.Jørgensen Xinglin Jiang Fernando Roman-Hurtado Fernando Reyes Olga Genilloud Tilmann Weber Fundacion MEDINA Centro de Excelencia en Investigaci´on de Medicamentos Innovadores en AndalucíaAvda del Conocimiento 3418016ArmillaGranadaSpain The Novo Nordisk Foundation Center for Biosustainability Technical University of DenmarkKemitorvetbuilding 2202800 Kgs.LyngbyDenmark

Globomycin is a cyclic lipodepsipeptide originally isolated from several Streptomyces species which displays strong and selective antibacterial activity against Gram-negative *** mode of action is based on the competitive inhibition of the lipoprotein signal peptidase II(LspA),which is absent in eukaryotes and considered an attractive target for the development of new *** its interesting biological properties,the gene cluster encoding its biosynthesis has not yet been *** this study we employed a genome-mining approach in the globomycin-producing Streptomyces ***-278952 to identify a candidate gene cluster responsible for its biosynthesis.A null mutant was constructed using CRISPR base editing where production was abolished,strongly suggesting its involvement in the *** putative gene cluster was then cloned and heterologously expressed in Streptomyces albus J1074 and Streptomyces coelicolor M1146,therefore unambigu-ously linking globomycin and its biosynthetic gene *** work paves the way for the biosynthesis of new globomycin derivatives with improved pharmacological properties.

关键词： Globomycin Signal peptidase II Cyclic lipodepsipeptide Genome mining CRISPR-cBEST Heterologous expression

Assessment of the Connection of Antisocial-Criminal Behavior, Corruption and Depression in College Students

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Psychology Research 2017年第3期7卷 131-139页

作者： Iris Xochitl Galicia Moyeda Alejandra Sanchez Velasco francisco javier Robles Ojeda Universidad Nacional Autonoma de Mexico Iztacala Mexico

Promising diagnostic biomarkers of nonalcoholic fatty liver disease and nonalcoholic steatohepatitis: From clinical proteomics to microbiome

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World Journal of Hepatology 2021年第11期13卷 1494-1511页

作者： Carolina Castillo-Castro Alexandro JoséMartagón-Rosado Rocio Ortiz-Lopez Luis Felipe Garrido-Treviño Melissa Villegas-Albo francisco javier Bosques-Padilla Tecnológico de Monterrey Escuela de Medicina y Ciencias de la SaludMonterrey 64710Mexico Unidad de Investigación de Enfermedades Metabólicas Instituto Nacional de Ciencias Médicas y NutriciónCiudad de México 14080Mexico Centro Regional para el Estudio de las Enfermedades Digestivas Servicio de GastroenterologíaFacultad de Medicina y Hospital Universitario Dr.JoséEleuterio GonzálezUniversidad Autónoma de Nuevo LeónMonterrey 64460Mexico

Fatty liver has been present in the lives of patients and physicians for almost two *** knowledge has been generated regarding its etiology and consequences,although a long path seeking novel and innovative diagnostic biomarkers for nonalcoholic fatty liver disease(NAFLD)and nonalcoholic steatohepatitis(NASH)is still *** the one hand,proteomics and lipidomics have emerged as potential noninvasive resources for NAFLD *** contrast,metabolomics has been able to distinguish between NAFLD and NASH,even detecting degrees of *** the other hand,genetic and epigenetic markers have been useful in monitoring disease progression,eventually functioning as target *** markers involved in immune dysregulation,oxidative stress,and inflammation are involved in the instauration and evolution of the ***,the fascinating gut microbiome is significantly involved in NAFLD and *** review presents state-of-the-art biomarkers related to NAFLD and NASH and new promises that could eventually be positioned as diagnostic resources for this *** is evident,despite great advances in studying these biomarkers,there is still a long path before they translate into clinical benefits.

关键词： Fatty liver Biomarkers Nonalcoholic fatty liver disease Nonalcoholic steatohepatitis

mRNA levels of TLR4 and TLR5 are independent of H pylori

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World Journal of Gastroenterology 2008年第34期14卷 5306-5310页

作者： Elvira Garza-González Virgilio Bocanegra-García francisco javier Bosques-Padilla Juan Pablo Flores-Gutiérrez francisco Moreno Guillermo Ignacio Perez-Perez Departmento de Microbiología Facultad de MedicinaUniversidad Autónoma de Nuevo León Departmento de Biología Molecular y Bioingeniería UAM Reynosa Aztlán Universidad Autónoma de Tamaulipas Hospital Universitario "Dr. José Eleuterio González" Universidad Autónoma de Nuevo León Department of Medicine and Microbiology New York University School of Medicine

AIM:To determine if the presence H pylori or its viru- lence affect toll-like receptor 4 (TLR4) and TLR5 mRNA expression levels. METHODS:For the in vivo assays, gastric biopsies were obtained from 40 patients and H pylori status was determined. For the in vitro assays, human gastric adenocarcinoma mucosal cells (AGS) were cultured in the presence or absence of twelve selected H pylori strains. H pylori strains isolated from culture-positive patients and selected strains were genotyped for cagA and vacA. The cDNA was obtained from mRNA extracted from biopsies and from infected AGS cells. TLR4 and TLR5 mRNA levels were examined by real-time PCR. RESULTS: The presence of H pylori did not affect the mRNA levels of TLR4 or TLR5 in gastric biopsies. The mRNA levels of both receptors were not influenced by the vacA status (P > 0.05 for both receptors) andthere were no differences in TLR4 or TLR5 mRNA levels among the different clinical presentations/histological fi ndings (P > 0.05). In the in vitro assay, the mRNA levels of TLR4 or TLR5 in AGS cells were not influenced by the vacAs1 status or the clinical condition as-sociated with the strains (P > 0.05 for both TLR4 and TLR5). CONCLUSION: The results of this study show that the mRNA levels of TLR4 and TLR5 in gastric cells, both in vivo and in vitro, are independent of H pylori colonization and suggest that vacA may not be a significant player in the first step of innate immune recognition mediated by TLR4 or TLR5.

关键词： H pylori Toll-like receptor 4 Toll-likereceptor 5 AGS cells mRNA