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Fumonisin-Producing Fusarium from Maize Grains in Tretep, Indonesia

Journal of Food Science and Engineering 2013年第10期3卷 534-540页

作者： Petrus Adityo Nugroho francis maria constance sigit setyabudi Baharudin Saleh Endang SutriswatiRahayu Faculty of Agricultural Technology Universitas Gadjah Mada Bulaksumur Yogyakarta Indonesia School of Biological Science Universiti Sains Malaysia Penang Malaysia

Fusarium species commonly occur in maize are fungal pathogen which produce mycotoxins, such as fumonisin, trichothecene and zearalenone. In this study, Fusarium species were isolated from maize kernel from Tretep, maize producer region and were identified based on microscopic- and macroscopic characters as well as molecular characters using PCR assays and the partial sequence of TEF 1-α gene （Translation Elongation Factor 1-α. The fumonisin-producing ability of these Fusarium was determined by growing them in corn medium and analyzed their fumonisin by ELISA （enzyme-linked immuno assay）. Among 9 isolates, three of them were identified as Fusarium verticillioides, two as Fusarium temperatum, two as Fusarium globosum, one as Fusarium proliJeratum and one as Fusarium subglutinans. Fusarium temperatum is similar morphologically to F. subglutinans, however, both of their differences can be found by molecular analysis. Fumonisin-producing abilities of Fusarium were determined in concentrations 20.51 pg/g-1,109.74 pg/g medium with the highest producer was identified as F. globosum.

关键词： Fusarium tretep identification molecular TEF-1α fumonisin.

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Ochratoxigenic Black Aspergilli Isolated from Dried Agricultural Products in Yogyakarta, Indonesia

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Journal of Food Science and Engineering 2013年第9期3卷 472-480页

作者： Apriyanto Dwi Nugroho francis maria constance sigit setyabudi Baharuddin Salleh Endang Sutriswati Rahayu Faculty of Agricultural Technology Universitas Gadjah Mada Yogyakarta Indonesia School of Biological Science Universiti Sains Malaysia Penang Malaysia

Black aspergilli, the potential ochratoxin A （OTA） producers, were predominant fungi in fermented cocoa bean, coffee bean and dried cassava in Yogyakarta. Identification of black aspergilli at species level will be useful to make clear link between OTA contamination on food product and the toxin producer. The objective of this study was to identify the species of the black aspergilli producing OTA which contaminated dried agriculture products. In this study, 16 isolates were obtained, and four isolates among of them （YAC-9, YAK-6, YAK-12 and YAG-2） were found as OTA producing-strains, with the highest OTA found on Yeast Extract Sucrose （YES） solid medium of 57.68 ppb. Based on morphological characters, 16 isolates can be grouped into four species, after confirmation by molecular data based on PCR method, the groups were identified as A. carbonarius, A. niger, A. tubingensis and A. aculeatus. OTA producing-strains were identified as A. carbonarius and A. niger, meanwhile, A. tubingensis and A. aculeatus were found as non-OTA producing-strains.

关键词： Black aspergilli ochratoxin A (OTA) PCR.

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