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Justicia insularis Improves the in vitro Survival and Development of Ovine Preantral Follicles Enclosed in Ovarian Tissue

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Journal of Pharmacy and Pharmacology 2017年第9期5卷 668-680页

作者： Gildas Tetaping Mbemya Denise Damasceno Guerreiro Nathalie Jiatsa Donfacl~ Luciana Mascena Silv~ Luis Alberto Vieira Geovania Francisca Canafistula de Sousa Benner Geraldo Alves Aryele Pinto Izaguirry francielli weber santos Phelix Bruno Telefo Otilia Deusdenia Loiola Pessoa Johan Smitz Jose Ricardo de Figueiredo Ana Paula Ribeiro Rodrigues Faculty of Veterinary (FA VET) State University of Ceara Fortaleza 60714903 Brazil Laboratory of Reproduction Biotechnology (Biotech) State of University of Pampa Uruguaiana 97500970 Brazil Laboratory of Biochemistry of Medicinal Plants Food and Nutritional Sciences Faculty of Science University of DschangDschang 3451381 Cameroon Laboratory of Phytochemical Analysis of Medicinal Plants (LAFIPLAN) Federal University of Cear6 Fortaleza 60020181 Brazil Follicle Biology Laboratory Center for Reproductive Medicine Brussels 1090 Belgium

Objectives： Evaluating the addition effect of./. insularis extract and FSH on the survival, activation and ROS production after in vitro culture of ovine preantral follicles enclosed in ovarian tissue. Methods： In the first experiment, ovarian fragments were fixed （non-cultured control） or in vitro cultured in α-MEM＋（cultured control）, α-MEM＋ supplemented with FSH 50 ng/mL, or in α-MEM＋supplemented with J. insularis （JUS0.3; 1.25 or 5 mg/mL） for 1 or 7 days, at 39℃, 5% CO2. In the second experiment, fragments were fixed or cultured in α-MEM＋ supplemented with anethole 300 μg/mL ＋ FSH 50 ng/mL or in α-MEM＋ supplemented with anethole 300μg/mL ＋ 0.3 mg/mL JUS. Key findings： JUS0.3 was the only treatment that maintained the percentage of morphologically normal follicles similar to non-cultured control even after 7 days of culture. After 7 days of culture, a higher （p 〈 0.05） percentage of developing follicles was observed in JUS5 treatment compared with the other treatments except JUS 1.25. In the second experiment, FSH maintained the percentage of normal follicles and promoted activation of primordial follicles. A reduction （p 〈 0.05） of stromal cell density was observed in MEM＋＋ANE supplemented with JUS or FSH. Conclusions： J. insularis in a concentration-dependent manner maintained the levels of ROS and improved in vitro follicular survival and activation of ovine primordial follicles.

关键词： Medicinal plant antioxidant in vitro folliculogenesis preantral follicles.

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Composition of Cachacas Produced from Five Varieties of Sugarcane and the Correlation of the Presence of Dhurrin in the Cane with That of Ethyl Carbamate in the Product

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American Journal of Plant Sciences 2019年第2期10卷 339-350页

作者： francielli D’Carlos Cravo Wilder Douglas Santiago Allan da Silva Lunguinho Richard Bispo Barbosa Renan Elan da Silva Oliveira Gabriela Fontes Alvarenga Stephano Daniel santos Rafaella Hilda Zaniti Souza Ellen Cristina de Souza Katia Julia de Almeida Josefina Aparecida de Souza David Lee Nelson Maria das Gracas Cardoso Department of Food Science Federal University of LavrasC.P.LavrasMinas GeraisBrazil Department of Chemistry Federal University of LavrasC.P.LavrasMinas GeraisBrazil Pro-Reitoria de Pesquisa e Pos-Graduacao Federal University of Vales de Jequitinhonha and MucuriDiamantinaMGBrazil

The present work sought to characterize the cyanogenic glycoside dhurrin in five sugarcane varieties and to determine its possible relationship with the formation of ethyl carbamate in cachaca. For each variety, methanol, ethyl acetate and hexane extracts were prepared and submitted to thin layer chromatography. Chromatographic spots were revealed with iodine vapors. The physical and chemical parameters of the spirits produced from the five different varieties of sugar cane and by different production processes were within the legal limits. Although not exceeding the limit for ethyl carbamate, a significant variation in the final concentration of this compound was observed for each sample analyzed. The Rf values for the dhurrin standard and for the sugarcane variety SP 83-2847 were equal, similar to those for SP 80-3280 and CTC 11 and different from the Rf values for the RB 86-7515 and IAC 86-2480 samples.

关键词： Cachaca Ethyl Carbamate Quality Glycosides Cyanide Dhurrin

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