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A Girl with Life Threatening Meningitis Caused by an Infected Dermoid Cyst Derived from an Occult Dermal Sinus

Case Reports in Clinical Medicine 2014年第8期3卷 450-456页

作者： Valentina Tono Sara Bertoletti Francesca Maltese francesco canonico Erik Sganzerla Carlo Giussani Maria Luisa Melzi Andrea Biondi Department of Diagnostic and Interventional Radiology Ospedale S. Gerardo Monza Italy Department of Neurosurgery Ospedale S. Gerardo Monza Italy Department of Pediatrics University of Milano-Bicocca Fondazione MBBM Monza Italy

Congenital dermal sinuses are a rare entity of spinal dysraphism. Most patients have cutaneous markers of their presence, including nevus or port wine stains, dimple, hypertrichosis and subcutaneous lipoma. Inclusion tumors, such as dermoid tumor, are associated to dermal sinus tract;they are congenital, benign and slow-growing lesions of midline. Their clinical relevance is due to the possibility of causing infection, spinal abscess, signs of corde or nerve root compression. Spinal Magnetic Resonance Imaging (MRI) is the diagnostic tool of choice. We present the case of a child with atypical meningitis, resistant to large spectrum antibiotic therapy, caused by an infected dermoid cyst, undiagnosed in the first months of life despite repeated MRI.

关键词： Dermal Sinus Meningitis Dermoid Cyst Spinal Dysraphism

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Role of ileostomy in restorative proctocolectomy

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World Journal of Gastroenterology 2012年第15期18卷 1703-1707页

作者： Gianluca Pellino Guido Sciaudone Silvestro canonico francesco Selvaggi Division of General Surgery Second University of Naples42-80122 NaplesItaly

Restorative proctocolectomy(RP) is the treatment of choice in patients affected with refractory ulcerative colitis or familial adenomatous *** in elective settings is often performed in 2 stages,fashioning an ileostomy which is closed 2-3-mo *** is still debated whether omitting ileostomy could offer advantages in the management of patients undergoing RP.

关键词： Restorative proctocolectomy Ileostomy Ulcerative colitis Familial adenomatous polyposis Ileopouch-anal anastomosis

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Stepwise approach and surgery for gallbladder adenomyomatosis: a mini-review

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Hepatobiliary & Pancreatic Diseases International 2013年第2期12卷 136-142页

作者： Gianluca Pellino Guido Sciaudone Giuseppe Candilio Giuseppe Perna Antonio Santoriello Silvestro canonico francesco SelvaggiAuthor Unit of General and Geriatric Surgery School of Medicine Second University of Naples

BACKGROUND: Gallbladder adenomyomatosis (GBA) is a hyperplastic disease affecting the wall of the gallbladder, with some typical features. It has historically been considered a benign condition, nevertheless recent reports highlighted a potential role of GBA in predisposing to malignancies of the gallbladder. DATA SOURCES: We reviewed the literature concerning GBA from its identification until July 2012. Owing to the relative rarity of the disease, studies often are case reports or case series. Thus we herein report a summary of the key-points concerning diagnosis and treatment of GBA, easily applicable in everyday practice, rather than a systematic review. Also, results are integrated with our recent experience. RESULTS: In our experience, we observed a trend toward an increase of GBA during the last years, probably due to enhanced ultrasonographic technical advancements and physician’s expertise. GBA has distinctive imaging features. Several recent reports highlight the potential risk of cancer associated with GBA; however the disease is still classified as a benign condition. Although its correlation with malignancy has not been demonstrated, it is prudent to recommend cholecystectomy in some cases. However, in selected asymptomatic patients, a wait-and-see policy is a viable alternative. We propose an algorithm, based on GBA pathological pattern (diffuse, segmental, localized or fundal), suitable for ***: In symptomatic patients and if the diagnosis is doubtful, cholecystectomy is mandatory. Postponing surgery is an option to be offered to asymptomatic patients with low-risk GBA pattern who adhere to scheduled follow-ups.

关键词： gallbladder adenomyomatosis gallbladder carcinoma cholecystectomy ultrasonography

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Inter-Laboratory Ring Trial to Evaluate Reverse Transcription Polymerase Chain Reaction Methods Used for <i>Dolphin Morbillivirus</i>Detection in Italy

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Advances in Microbiology 2019年第5期9卷 423-437页

作者： Zoccola Roberto Pautasso Alessandra Grattarola Carla Monnier Milena Miceli Ilaria Ingravalle francesco Crescio Maria Ines Giorda Federica Masoero Loretta Zaccaria Guendalina Purpari Giuseppa Cersini Antonella Viscardi Maurizio Serracca Laura Padalino Iolanda Puggioni Giantonella canonico Cristina Toffan Anna Centelleghe Cinzia Di francesco Cristina Esmeralda Mazzariol Sandro Di Guardo Giovanni Mignone Walter Casalone Cristina Goria Maria Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d’Aosta Torino Italy Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise Teramo Italy Istituto Zooprofilattico Sperimentale della Sicilia Palermo Italy Istituto Zooprofilattico Sperimentale del Lazio e della Toscana Roma Italy Istituto Zooprofilattico Sperimentale del Mezzogiorno Portici (NA) Italy Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d’Aosta La Spezia Italy Istituto Zooprofilattico Sperimentale della Puglia e Basilicata Foggia Italy Istituto Zooprofilattico Sperimentale della Sardegna Sassari Italy Istituto Zooprofilattico Sperimentale dell’ Umbria e delle Marche Ancona Italy Istituto Zooprofilattico Sperimentale delle Venezie Legnaro (PD) Italy University of Padova Department of Comparative Biomedicine and Food Science (BCA) Legnaro (PD) Italy Faculty of Veterinary Medicine University of Teramo Teramo Italy Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d’Aosta Imperia Liguria Italy

Dolphin Morbillivirus (DMV) is one of the most frequently detected pathogens in stranded cetacean specimens worldwide as well as in Italy. Due to the persistence of DMV in the Mediterranean Sea and to the lack of information about the efficiency of the available diagnostic techniques, the Italian National Reference Centre for diagnostic activities on dead stranded marine mammals (***) performed the first inter-laboratory ring trial with the aim to standardize a diagnostic biomolecular approach for DMV in Italy. Viral isolation is usually considered the “gold standard” for the definitive diagnosis of most pathogens, but it is not often feasible in DMV diagnosis, due to the poor preservation of virus-targeted tissues in stranded cetacean carcasses, as well as to the lack of appropriate sensitivity of cell lines towards DMV variability. Therefore direct viral detection on tissues by means of reverse transcription-PCR (RT-PCR) represents a valuable option for DMV infection’s diagnosis. For detecting DMV in cetacean die-offs occurred in the Mediterranean basin since 2013, *** developed an RT-PCR based method targeting to a 287 bp fragment of DMV nucleoprotein (N) gene. With the purpose to evaluate its performances in terms of accuracy (Se = sensitivity and Sp = specificity) and precision (reproducibility), it was submitted to a ring trial. So, 12 Public Laboratories belonging to the Italian dead stranded marine mammals diagnostic network were asked to analyze a panel of 40 samples (positive and negative for DMV, using different dilutions of a viral suspension obtained from a cell culture supernatant of a DMV strain) with the aforementioned technique. Furthermore, we also aimed at comparing the accuracy of other 7 molecular methods routinely applied for DMV detection in Italy. For this purpose, the second panel of identical 40 DMV +ve and -ve samples was provided to Laboratories that routinely used DMV detection methods other than those developed by ***.

关键词： Dolphin Morbillivirus RT-PCR Interlab Ring Test Method Comparison Stranded Cetaceans Mediterranean Sea

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