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Asian Journal of Andrology 2014年第6期16卷 838-844页

作者： Zaida Sarrate francesca vidal Joan Blanco Unitat de Biologia Cel.lular Facultat de Biociencies Universitat Autonoma de Barcelona Bellaterra (Cerdanyola del Valles) 08193 Spain.

The aim of this study was to look in depth at the relationship between meiotic anomalies and male infertility, such as the determination of the chromosomes involved or the correlation with patient features. For this purpose, a total of 31 testicular tissue samples from individuals consulting for fertility problems were analyzed. Metaphase I cells were evaluated using a sequential methodology combining Leishman stained procedures and multiplex fluorescence in situ hybridization protocols. The number of chromosomal units and chiasmata count per bivalent were established and a hierarchical cluster analysis of the individuals was performed. The relationship of the seminogram and the karyotype over recombination were evaluated using Poisson regression models. Results obtained in this study show a significant percentage of infertile individuals with altered meiotic behavior, mostly specified as a reduction in chiasmata count in medium and large chromosomes, the presence of univalents, and the observation of tetraploid metaphases. Moreover, the number and the type of anomalies were found to be different between cells of the same individual, suggesting the coexistence of cell lines with normal meiotic behavior and cell lines with abnormalities. In addition, chromosomal abnormalities in metaphase I are significantly associated with oligozoospermia and/or polymorphic karyotype variants.

关键词： chiasmata count human spermatocytes multiplex fluorescence in situ hybridization polymorphic karyotype seminalparameters

A comprehensive analysis of chromosomal anomalies in metaphase II spermatocytes from infertile patients

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Asian Journal of Andrology 2018年第1期20卷 105-106页

作者： Zaida Sarrate Joan Blanco Oliver Valero francesca vidal Genetics of Male Fertility Group Unitat de Biologia Cel.lular Departament de Biologia Cel.lular Fisiologia i Immunologia Facultat de Biociencies Universitat Autonoma de Barcelona Bellaterra (Cerdanyola del Valles) 08193 Spain Servei d'Estadistica Aplicada Universitat Autonoma de Barcelona Bellaterra (Cerdanyola del Valles) 08193 Spain

Dear Editor, The scarce number of secondary spermatocytes in human seminiferous tubules and the complexity of analyzing chromosomes at metaphase II are the main causes of the limited knowledge we have about chromosome characteristics at this stage. In this study, we have performed a comprehensive analysis of meiotic abnormalities in metaphase II spermatocytes combining Leishman＇s staining protocols and multiplex fluorescent in situ hybridization procedures. Results indicated that infertile individuals showed different susceptibilities to meiotic abnormalities in secondary spermatocytes without a preferentially effect on any particular chromosome.

关键词： MEIOTIC ABNORMALITIES SISTER CHROMATIDS ANEUPLOIDY MEIOSIS SEPARATION OOCYTES MALES

Unpaired sex chromosomes in metaphase I human spermatocytes locally modify autosomal bivalents positioning

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Asian Journal of Andrology 2018年第6期20卷 626-628页

作者： Carla Mayans Joan Blanco Oliver Valero francesca vidal Zaida Sarrate Departament de Biologia Cel.lular Fisiologia i Immunologia Facultat de Biociencies Universitat Autbnoma de Barcelona Bellaterra (Cerdanyola del Valles) 08193 Spain Servei d'Estadistica Aplicada Universitat Autonoma de Barcelona Bellaterra (Cerdanyola del Valles) 08193 Spain

Dear Editor, In interphase nuclei, chromosomes occupy specific localizations called chromosome territories （CTs）. Distribution of CTs has been related to chromosome size and gene density and is known to influence gene expression.~ This pattern of organization appears to be a widespread nuclear feature, both in somatic and germ ceils.

关键词： ABNORMALITIES

Re： Is number of chiasmata an etiological factor of male infertility？

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Asian Journal of Andrology 2014年第6期16卷 921-921页

作者： Zaida Sarrate francesca vidal Joan Blanco Unitat de Biologia Cel-lular Facultat de Biociencies Universitat Autonoma deBarcelona Bellaterra (Cerdanyola del Valles) 08193 Spain.

We would like to thank Kurpisz and Olszewska for their commentary on our article2 and we welcome the opportunity to discuss some of the issues addressed by our colleagues.

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