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m^(6)A Profile Dynamics Indicates Regulation of Oyster Development by m^(6)A-RNA Epitranscriptomes

Genomics, Proteomics & Bioinformatics 2023年第4期21卷 742-755页

作者： Lorane Le franc Bruno Petton Pascal Favrel Guillaume Rivière Laboratoire de Biologie des Organismes et des Ecosyste`mes Aquatiques(BOREA) Muséum d’Histoire NaturelleSorbonne UniversitéUniversitéde Caen NormandieUniversitédes AntillesCNRS UMR 8067IRD14032 CaenFrance Ifremer Laboratoire des Sciences de l’Environnement MarinUMR 6539 CNRS/UBO/IRD/IfremerCentre Bretagne29280 PlouzanéFrance

The N6-methylation of RNA adenosines(N6-methyladenosine,m^(6)A)is an important regulator of gene expression with critical implications in vertebrate and insect ***,the developmental significance of epitranscriptomes in lophotrochozoan organisms remains *** methylated RNA immunoprecipitation sequencing(MeRIP-seq),we generated transcriptome-wide m^(6)A-RNA methylomes covering the entire development of the oyster from oocytes to *** RNA classes display specific m^(6)A signatures,with messenger RNAs(mRNAs)and long non-coding RNAs(lncRNAs)exhibiting distinct profiles and being highly methylated compared to transposable element(TE)*** are dynamic and correspond to the chronological steps of development(cleavage,gastrulation,organogenesis,and metamorphosis),with minimal mRNA and lncRNA methylation at the morula stage followed by a global *** m^(6)A levels are correlated with transcript levels,and shifts in methylation profiles correspond to expression *** methylated transcripts cluster according to embryo-larval stages and bear the corresponding developmental functions(cell division,signal transduction,morphogenesis,and cell differentiation).The m^(6)A level of TE transcripts is also regulated and peaks during the *** demonstrate that m^(6)A-RNA methylomes are dynamic and associated with gene expression regulation during oyster *** putative epitranscriptome implication in the cleavage,maternal-to-zygotic transition,and cell differentiation in a lophotrochozoan model brings new insights into the control and evolution of developmental processes.

关键词： RNA methylation Transcription Embryo Metamorphosis Evo-devo

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solidification of Be-free Ni-based dental alloy

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Transactions of Nonferrous Metals society of china 2018年第11期28卷 2227-2236页

作者： franc ZUPANIc carlos A.NUNEs Gilberto c.cOELHO Paula L.cURy Gorazd LOJEN Tonica BONcINA Faculty of Mechanical Engineering University of Maribor2000 MariborSlovenia Department of Materials Engineering School of EngineeringUniversity of Sao Paulo12602-810 LorenaSao PauloBrazil

A novel, Nb- and si-rich and Be-free Ni-based alloy was cast by two methods of investment casting and continuouscasting to study the microstructure evolution during solidification and its mechanical properties. The solidification of the alloy startedwith the primary crystallization of Fcc-γ, followed by a binary eutectic reaction, with the formation of a heterogeneous constituent：Fcc-γ＋G-phase, which replaced the low-melting eutectic （Fcc-γ＋NiBe） in the Be-bearing alloys. AlNi6si3 and γ′ formed during theterminal stages of solidification by investment casting, while the formation of AlNi6si3 was suppressed by continuous casting. Thescheil solidification model agreed very well with the experimental results.

关键词： solidification nickel alloy continuous casting investment casting microstructure dental alloy

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Aboveground biomass allometric equations and distribution of carbon stocks of the African oak(Afzelia africana sm.)in Burkina Faso

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Journal of Forestry Research 2020年第5期31卷 1699-1711页

作者： Larba Hubert Balima Blandine Marie Ivette Nacoulma Philippe Bayen Kangbeni Dimobe franc¸ois N’Guessan Kouame Adjima thiombiano West African science service centre on climate change and Adapted Land Use(WAscAL) Graduate Research Program on Climate Change and BiodiversityUFR BiosciencesUniversity Felix Houphouet BoignyB.P.165Abidjan 31Cote d’Ivoire Department of Plant Biology and Physiology Laboratory of Plant Biology and EcologyUniversity Ouaga 1Pr.Joseph Ki-Zerbo03 B.P.7021Ouagadougou 03Burkina Faso Laboratory of Botany and Plant Ecology Department of BotanyUniversity of LomeB.P.1515LomeTogo

The significant role of tropical forest ecosystems in the global carbon budget has increased the need for accurate estimates of tropical forest *** lack of large-scale biomass allometric equations hampers the understanding of the spatial distribution of tree biomass and carbon stocks and their influencing factors in West *** study aimed to develop allometric equations to estimate aboveground biomass of African oak(Afzelia africana sm.)in Burkina Faso and to analyze factors affecting the variability of tree biomass and carbon *** individual trees were destructively sampled in four protected areas along two climatic *** each climatic zone,log–log models were tested and fitted to each aboveground biomass component and to the total aboveground *** content in tree aboveground components was evaluated using the ash *** validated equations showed good fit and performance with high explained *** equations differed between the sudano-sahelian zone and the sudanian zone,except for leaf biomass *** biomass allocation and carbon content varied significantly between tree components but not between climatic *** content in tree components followed the patterns of biomass allocation with branches accounting for the highest *** the two climatic zones,carbon contents were 50.18–52.62%for leaves,54.78–54.94%for stems and 54.96–55.99%for *** biomass ranged from 509.05 to 765.56 kg tree^-1 at site level and from 620.21 to 624.48 kg tree^-1 along climatic *** content varied from 53.90%in the sudano-sahelian zone to 54.39%in the sudanian *** study indicated that climate does not influence aboveground biomass production and carbon sequestration of Afzelia africana along the sudanosahelian and the sudanian climatic zones of Burkina *** studies on climate–growth relationships should contribute to better understanding climate effects on biomass production and carbon s

关键词： African mahogany Biomass allometry carbon storage climatic gradient West Africa

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children safety Devices in Brazil—Why Do People Don’t Use Them after the Law?

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Journal of Transportation Technologies 2014年第3期4卷 205-215页

作者： s.c.V.Abib A.M.francóia F.Ricci M.V.B.cezillo B.R.Müller Department of surgery Paulista School of MedicineFederal University of Sao PauloSao PauloBrazil criança segura—safe Kids Brazil Sao PauloBrazil

Introduction: children safety car devices decrease injuries and death in children. A survey conducted in Brazil in 2009 revealed that only 36.1% of children safely transported. In 2010, a Brazilian law was implemented, obligating the use of safety devices. The aim of this study is to evaluate the use of such devices after the law and the eventual reasons for non-use. Methods: A significant sample of Brazilian population aged 18 years or more, which normally carry children until 10 years old answered a survey between August 16 and 25, 2012. The study was conducted in two phases. The first one aimed to raise the proportion and profile of the target population, while the second investigated the children car safety device use (or not). Results: 622 interviews. children’s transporters are young males (57%), living in a metropolitan area in the south region, concerned about safety and law supervision, with greater education level and income than non-transporters (31%) who are male with lack of information, living in a non-metropolitan area of the Northeast region that would be motivated to use the device by effective law fiscalization or threat of an accident. conclusion: Two years after the law it is observed that education, income, age, gender and region of origin influence the use/non-use of the safety seats in Brazil. The accident threat and fiscalization are the main reasons for using the device, showing that the lack of information impairs the democratization of its use and the prevention culture has to be enhanced in the country.

关键词： child Restraint systems Wounds and Injuries Accidents Traffic Pediatrics

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迷迭香(Rosmarinus officinalis)提取残渣栽培糙皮侧耳(英文)

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食用菌学报 2008年第2期15卷 15-19页

作者： Andrej GREGORI Bojan PAHOR franc POHLEVEN 自然科学研究所 SRC Bistra PtujSlovenski trg 62250 PtujUlica bratov Ucakar 1081000 LjubljanaBiolab d.o.o.Podkoren 724280 Kranjska goraSlovenia Perutnina Ptuj d.d. Potrceva cesta 102250 PtujSlovenia 卢布而雅那大学生物技术学院木材科学与技术系 Ro na dolinaCesta VIII/341000 LjubljanaSlovenia

食品工业中用迷迭香提取抗氧化物质后的残渣(rosemary processing waste RPW)拌入粉碎的玉米粒可以用作栽培糙皮侧耳的培养料。提取抗氧化剂后的迷迭香残渣RPW-1中所含提取残留的丙酮对栽培有害,RPW-1直接栽培糙皮侧耳所获子实体产量比... 详细信息

食品工业中用迷迭香提取抗氧化物质后的残渣(rosemary processing waste RPW)拌入粉碎的玉米粒可以用作栽培糙皮侧耳的培养料。提取抗氧化剂后的迷迭香残渣RPW-1中所含提取残留的丙酮对栽培有害,RPW-1直接栽培糙皮侧耳所获子实体产量比用RPW-2(废料充分通气,去除残存的丙酮)平均低49%。试验中获得最高生物效率(67%)的培养料配比为RPW-2中加入30%(w/w)粉碎的玉米粒(crushed corn seeds ccs)和2%cacO3。增加培养料中的麸皮(wheat bran WB)比例(降低c/N比)可加快菌丝生长速度,但通常导致子实体产量降低。试验中菌丝生长最快(23 d生长9.9 cm)的培养料配比为RPW-2中添加30%(w/w)ccs和30%(w/w)WB,c/N比为24。

关键词：农业副产品食用菌栽培糙皮侧耳迷迭香提取残渣

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Assessing potential protection effects on commercial fish species in a cuban MPA

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Aquaculture and Fisheries 2020年第5期5卷 234-244页

作者： Barbara Horta e costa Jorge Angulo-Valdés Jorge M.s.Gonçalves Pedro Barros center of Marine sciences CCMARUniversity of AlgarveCampus de GambelasFaro8005-139Portugal centro de Investigaciones Marinas Universidad de La HabanaCalle 16 No.114PayaLa HabanaCP 11300Cuba school of Natural Resources and Environment University of FloridaUnited States of America103 Black HallGainesvilleFL32611USA centre for Marine and Environmental Research(cIMA) University of AlgarveCampus de GambelasFaro8005-139Portugal Food and Agriculture Organization(FAO) Fisheries and Aquaculture Department Marine and Inland Fisheries Branch(FIRF)Rome00153Italy

cuba has been leading marine protected area(MPA)designation in the caribbean region to ensure conservation of its valuable marine ***,an efficient monitoring program for MPAs is still to be implemented and will benefit from pre-existing *** highly regulated MPA of Punta franc.s National Park is one of the first cuban MPAs and was established mainly to protect species and ecosystems for tourism *** of protection effects on commercial fish species was lacking in this *** study aimed at increasing local scientific knowledge by providing a baseline study about the most commercially fished families(Haemulidae,Lutjanidae and serranidae)around Punta franc.s *** collected represent only a limited period but can be used as a base point to support future *** abundance in number and biomass,and size were collected to test for differences between inside and adjacent areas outside the Punta franc.s MPA,in different coral reef *** main result of this study was the significantly larger size and biomass of snappers and groupers outside the MPA where intense fisheries *** observation consistent with a large spawning aggregation was also recorded outside the *** with a limited set of data,these results suggest that,at least temporarily,the most targeted species[and sizes]are still highly vulnerable to *** habitats in the adjacent area,that are apparently missing within the MPA,may support some of the results *** the limits of the Punta franc.s MPA to include those habitats outside may be critical to ensure the effectiveness of this MPA in contributing to protect the most commercial species of the *** should be done together with efficient fisheries management measures in the region,such as the significantly increase of minimum legal sizes and temporal closures during spawning migrations.

关键词： cuba Marine protected areas commercial fish species spawning aggregations Artisanal fisheries coral reefs

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干扰素-a和利巴韦林治疗1例丙型肝炎合并皮肤结节性动脉周围炎患者(法语)

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世界核心医学期刊文摘（皮肤病学分册） 2006年第12期2卷 53-54页

作者： Naouri M. Bacq y. Machet M.c. L. Machet 王琼 service de Dermatologie Centre Hospitalier Universitaire UniversitéFrancis-Rabelais 37044 Tours Cedex France

Background. Hepatitis c virus (HcV) frequently causes leucocytoclastic vasculitis as a result of type II or III cryoglobulinemia. HcV-associated vasculitis without cryoglobulinemia is less common. Patients and methods. A 33-year-old woman consulted for infiltrative necrotic purpura of the lower limbs, responsible for leg ulcers measuring less than 1 cm. Histopathological examination revealed vasculitis affecting the hypodermic arterioles and caused by periarteritis nodosa. No extracutaneous involvement was observed. The patient had presented asymptomatic untreated HVc infection (genotype 3) for two years. Antiviral treatment resulted in elimination of the patient’s viremia and no relapse of skin lesions was observed two years after the end of treatment. comments. This patient presented vasculitis due to cutaneous nodular periarteritis associated with HVc without cryoglobulinemia. Hepatic impairment was mild and did not require any antiviral treatment. No further skin involvement was seen after treatment with colchicine and because the patient’s viral genotype was favorable, we decided to initiate antiviral therapy. This therapeutic approach should be considered by dermatologists, but it is nevertheless important to assess the risk of interferon-induced aggravation of vasculitis.

关键词：干扰素冷球蛋白血症 cV 抗病毒药结节性动脉周围炎坏死性动脉炎结缔组织疾病患者皮肤解剖部位

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妊高征后早期发生的代谢综合征

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世界核心医学期刊文摘（妇产科学分册） 2005年第9期1卷 60-61页

作者： Forese J.-c. Girouard J. MassèJ. y. Giguérè 高雪莲 cHUQ Centre de R echerche de l'Hǒpital St.-Franc.ois d'Assise Unitède Périnatalogie 10 Ru e de l'Espinay Quebec City Que. G1 L 3L5 Canada Dr.

OBJEcTIVE: The objective of the present study was to evaluate the cardiovasc ul ar risk profile and the prevalence of metabolic syndrome among women with a hist ory of pregnancy-induced hypertension (PIH). METHODs:From a cohort of 3,799 nul liparous women prospectively recruited between 1989 and 1997, we performed an ob servational study on 168 case-control pairs 7.8 years after delivery. Participa nts were scheduled for a visit with a research nurse to evaluate their cardiovas cular risk profile using a questionnaire, anthropometric measurements and blood specimen analysis. REsULTs: One hundred sixty-eight women with prior PIH (105 w ith gestational hypertension and 63 with preeclampsia) and 168 controls matched for age and year of index delivery were evaluated. The women with PIH (34.6±4.4 years) were more obese and had higher systolic (115 mm Hg versus 108 mm Hg) and diastolic (75 mm Hg versus 70 mm Hg) blood pressures (P co ntrols (35.1±4.5 years). They had lower high-density lipoprotein cholesterol l evel (1.30 mmol/L versus 1.42 mmol/L; P creased fasting blood glucose concentration (5.2 mmol/L versus 5.0 mmol/L; P = .002), and higher insulin leve ls (119 versus 91 pmol/L; P ce of the metabolic syndrome wa s higher in the PIH group (unadjusted odds ratio = 4.9; 95%confidence interval 2.1-10.9) compared with controls, even after adjustment for confounders (adjust ed odds ratio = 3.6; 95%confidence interval 1.4 -9.0). cONcLUsION: In white wo men in their mid-30s, the prevalence of the metabolic syndrome is 3-to 5-fold increased in those with a history of PIH in their first pregnancy. This emphasi zes the importance of long-term follow-up assessment for cardiovascular risk f actors in these women.

关键词：代谢综合征心血管疾病空腹血糖水平疾病危险因素初产妇先兆子痫人体测量混杂因素

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Long-term live-cell microscopy with labeled nanobodies delivered by laser-induced photoporation

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Nano Research 2020年第2期13卷 485-495页

作者： Jing Liu Tim Hebbrecht Toon Brans Eef Parthoens saskia Lippens chengnan Li Herlinde De Keersmaecker Winnok H.De Vos stefaan c.De smedt Rabah Boukherroub Jan Gettemans Ranhua Xiong Kevin Braeckmans Laboratory of General Biochemistry and Physical Pharmacy Faculty of PharmacyGhent UniversityGhent B-9000Belgium Department of Biomolecular medicine Faculty of Medicine and Health SciencesGhent UniversityGhent B-9000Belgium VIB-UGent center for Inflammation Research VIBGhent B-9000Belgium VIB Bioimaging core Ghent VIBGhent B-9000Belgium Department of Biomedical Molecular Biology Ghent UniversityGhent B-9000Belgium Univ.Lille CNRSCentrale LilleISENUniv.ValenciennesUMR 8520-IEMNLille F-59000Franc Laboratory of cell Biology and Histology Department of Veterinary SciencesUniversity of Antwerp2020 AntwerpBelgium centre for Advanced Light Microscopy Ghent UniversityGhent B-9000Belgium

Fluorescence microscopy is the method of choice for studying intracellular ***,its success depends on *** of specific and stable markers.A prominent example of markers that are rapidly gaining interest are nanobodies(Nbs.-15 kDa),which can be functionalized with bright and photostable organic *** to their relatively small size and high specificity,Nbs offer great potential for high-quality long-term subcellular imaging,but suffer from the fact that they cannot spontaneously cross the plasma membrane of live *** have recently discovered that laser-induced photoporation is well suited to deliver extrinsic labels to living cells without compromising their *** a laser-based technology,it is readily compatible with light microscopy and the typical cell recipients used for *** by these promising initial results,we demonstrate here for the first time successful long-term imaging of specific subcellular structures with labeled nanobodies in living *** illustrate this using Nbs that target GFP/yFP-protein constructs accessible in the cytoplasm,actin-bundling protein Fascin,and the histone H2A/H2B *** an efficiency of more than 80%labeled cells and minimal toxicity(-2%),photoporation proved to be an excellent intracellular delivery method for ***-lapse microscopy revealed that cell division rate and migration remained unaffected,confirming excellent cell viability and *** conclude that laser-induced photoporation labeled Nbs can be easily delivered into living cells,laying the foundation for further development of a broad range of Nbs with intracellular targets as a toolbox for long-term live-cell microscopy.

关键词： laser-induced photoporation vapor nanobubble long-term microscopy imaging nanobody intracellular delivery living cell labeling

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Turbo-taxonomy to assemble a megadiverse lichen genus:seventy new species of cora(Basidiomycota:Agaricales:Hygrophoraceae),honouring David Leslie Hawksworth’s seventieth birthday

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Fungal Diversity 2017年第3期84卷 139-207页

作者： Robert Lücking Manuela Dal Forno Bibiana Moncada Luis Fernando coca Leidy yasmín Vargas-Mendoza André Aptroot Laura Juliana Arias Betty Besal Frank Bungartz Diego Mauricio cabrera-Amaya Marcela E.s.cáceres José Luis chaves sionara Eliasaro Martha cecilia Gutiérrez Jesús E.Hernández Marin María de los Ángeles Herrera-campos María E.Holgado-Rojas Harald Jonitz Martin Kukwa Fabiane Lucheta santiago Madriñán Marcelo Pinto Marcelli suzana Maria de Azevedo Martins Joel A.Mercado-Díaz Jorge Alberto Molina Eduardo A.Morales Peter R.Nelson Freddy Nugra franc.sco Ortega Telma Paredes Ayda Lucía Patiño Rouchi Nadine Peláez-Pulido Rosa Emilia Pérez Pérez Gary B.Perlmutter Eimy Rivas-Plata Javier Robayo camilo Rodríguez Diego Fernando simijaca Edier soto-Medina Adriano Afonso spielmann Alejandra suárez-corredor Jean-Marc Torres carlos Alberto Vargas Alba yánez-Ayabaca Gothamie Weerakoon Karina Wilk Marcela celis Pacheco Mauricio Diazgranados Grischa Brokamp Thomas Borsch Patrick M.Gillevet Masoumeh sikaroodi James D.Lawrey Botanical Garden and Botanical Museum Berlin KöniginLuise-Straße 6-814195 BerlinGermany science Education The Field Museum1400 South Lake ShoreChicagoIL 60605USA Department of Botany Smithsonian InstitutionNational Museum of Natural History10th St Constitution Ave NWWashingtonDC 20560USA Department of Environmental science and Policy George Mason UniversityFairfaxVA 22030-4444USA Licenciatura en Biologı´a Universidad Distrital Francisco Jose´de CaldasCra.4 No.26D-54Torre de LaboratoriosHerbarioBogota´D.C.Colombia Herbario Universidad de caldas(FAUc) Edificio BicentenarioCalle 65No.26-10ManizalesA.A.275CaldasColombia ABL Herbarium G.v.d.Veenstraat 1073762 XK SoestThe Netherlands Biologı´a Universidad del Quindı´oCarrera 15 Calle 12 NorteArmeniaQuindı´oColombia 120 chavis Avenue LexingtonVA 24450USA charles Darwin Foundation Puerto AyoraSanta Cruz IslandGala´pagosEcuador schedestraße 8 53113 BonnGermany Jardı´n Bota´nico de Bogota´Jose´celestino Mutis Avenida Calle 63 No.68-95Bogota´D.C.Colombia Departamento de Biocieˆncias Universidade Federal de SergipeAvenida Vereador Olı´mpio Grandes/n.ItabaianaCEP:49.500-000 SergipeBrazil Laboratorio de Hongos Instituto Nacional de Biodiversidad(INBio)Apdo.22-3100Santo Domingo de HerediaCosta Rica Departamento de Botaˆnica Universidade Federal do Parana´Campus Polite´cnicoSetor de Cieˆncias Biolo´gicasAv.Cel.Francisco H.dos Santoss/nJardim das Ame´ricas81531-980 CuritibaPRBrazil Ingenierı´a Ambiental Universidad Distrital Francisco Jose´de CaldasCarrera 5 Este No.15-82Av.CircumvalarBogota´D.C.Colombia Instituto Experimental Jardı´n Bota´nico Dr.Tobı´as Lasser Centro de Investigacio´n y DesarrolloUniversidad Central de VenezuelaAve.Salvador AllendeJardı´n Bota´nico de CaracasApartado#2156Caracas 1010-AVenezuela Departamento de Bota´nica Instituto de Biologı´aUniversidad Nacional Auto´noma de Me´xicoApartado Postal 70-36704510 Ciudad De Me´xicoMexico Facultad de ciencias Escuela Profesional de Biologı´aUniv

Following a large-scale phylogenetic study of the lichenized genus cora(Basidiomycota:Agaricales:Hygrophoraceae),we formally describe 70 new species,honouring the seventieth birthday of David Leslie Hawksworth,one of the preeminent figures in mycology and lichenology in the past 50 *** on an updated phylogeny using the ITs fungal barcoding locus,we now recognize 189 taxa in a genus that until recently was considered to represent a single species;including this contribution,92 of these are formally recognized,including five taxa based on historical names or collections that have not been *** of cora can be recognized by a combination of morphological(size,colour,lobe configuration,surface hairs,hymenophore size and shape),anatomical(thallus thickness,cortex structure,photobiont type,hyphal papillae),and ecogeographical features(substrate,habitat,distribution),and a keytable allowing the identification of all accepted taxa is *** new species are:cora accipiter Moncada,Madrin˜a´n&Lücking ***.,*** Moncada,soto-Medina&Lücking ***.,*** Moncada,Dal Forno&Lücking ***.,*** Dal Forno,chaves&Lücking ***.,*** Moncada,***ı´guez&Lücking ***.,*** Lücking,Moncada&*** ***.,*** Moncada,ya´nez-Ayabaca&Lücking ***.,*** Moncada,Patin˜o&Lücking ***.,*** Lücking,***&Dal Forno ***.,*** Holgado,Rivas Plata&Perlmutter ***.,*** Dal Forno,Eliasaro&spielmann ***.,*** Nugra,Dal Forno&Lücking ***.,*** Lücking,Martins&Lucheta ***.,*** Moncada,R.-***´rez&Lücking ***.,*** Moncada,***.&Lücking ***.,*** Moncada,cabreraAmaya&Lücking ***.,*** Moncada,R.-***´rez&***.,*** Lücking,***&Dal Forno ***.,*** Moncada,***´rez-corredor&Lücking ***.,*** Moncada,***&Lücking ***.,***

关键词： Bolivia citizen science chile Mexico Puerto Rico sri Lanka

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