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A Short Amino-Terminal Part of Arabidopsis Phytochrome A Induces Constitutive Photomorphogenic Response

Molecular Plant 2012年第3期5卷 629-641页

作者： Andra＇s Viczia＇n E＇va Ada＇m Iris Wolf Ja＇nos Bindics Stefan kircher Marc Heijde Roman Ulm Eberhard scha＇fer Ferenc Nagy Institute of Plant Biology Biological Research Centre of the Hungarian Academy of Sciences Temesva＇ri krt. 62. H-6726 Szeged Hungary Biologie II/Institut for Botanik University of Freiburg Freiburg Dermany Department of Botany and Plant Biology University of Geneva Geneva Switzerland Centre for Biological Signalling Studies （BIOSS） University of Freiburg Freiburg Germany School of Biological Sciences University of Edinburgh Edinburgh UK

Phytochrome A （phyA） is the dominant photoreceptor of far-red light sensing in Arabidopsis thaliana, phyA accumulates at high levels in the cytoplasm of etiolated seedlings, and light-induced phyA signaling is mediated by a com- plex regulatory network. This includes light- and FHY1/FHL protein-dependent translocation of native phyA into the nucleus in vivo. It has also been shown that a short N-terminal fragment of phyA （PHYA406） is sufficient to phenocopy this highly regulated cellular process in vitro. To test the biological activity of this N-terminal fragment of phyA in planta, we produced transgenic phyA-201 plants expressing the PHYA406-YFP （YELLOW FLUORESCENT PROTEIN）-DD, PHYA406- YFP-DD-NLS （nuclear localization signal）, and PHYA406-YFP-DD-NES （nuclear export signal） fusion proteins. Here, we report that PHYA406-YFP-DD is imported into the nucleus and this process is partially light-dependent whereas PHYA406-YFP-DD-NLS and PHYA406-YFP-DD-NES display the expected constitutive localization patterns. Our results show that these truncated phyA proteins are light-stable, they trigger a constitutive photomorphogenic-like response when localized in the nuclei, and neither of them induces proper phyA signaling. We demonstrate that in vitro and in vivo PHYA406 Pfr and Pr bind COP1, a general repressor of photomorphogenesis, and co-localize with it in nuclear bodies. Thus, we conclude that, in planta, the truncated PHYA406 proteins inactivate COP1 in the nuclei in a light-independent fashion.

关键词： Arabidopsis cop phenotype phytochrome A COP1

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Shedding Light on the Crystallographic Etching of Multi-Layer Graphene at the Atomic Scale

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Nano Research 2009年第9期2卷 695-705页

作者： franziska Schäffel Jamie H.Warner Alicja Bachmatiuk Bernd Rellinghaus Bernd Büchner Ludwig Schultz Mark H.Rümmeli IFW Dresden P.O.Box 270116D-01171 DresdenGermany Department of Materials University of OxfordParks Rd.Oxford OX13PHUnited Kingdom

The controlled etching of graphite and graphene by catalytic hydrogenation is potentially a key engineering route for the fabrication of graphene nanoribbons with atomic *** hydrogenation mechanism,though,remains poorly *** this study we exploit the benefi ts of aberration-corrected high-resolution transmission electron microscopy to gain insight to the hydrogenation *** etch tracks are found to be commensurate with the graphite *** particles at the head of an etch channel are shown to be faceted and the angles between facets are multiples of 30°.Thus,the angles between facets are also commensurate with the graphite *** addition,the results of a post-annealing step suggest that all catalyst particles even if they are not involved in etching are actively forming methane during the hydrogenation ***,the data point against carbon dissolution being a key mechanism during the hydrogenation process.

关键词： Graphene graphene nanoribbons catalytic hydrogenation nanoparticles

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平台石墨炉原子吸收法测定血中银

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中华劳动卫生职业病杂志 1995年第4期13卷 241-242页

作者： scha,kH 常虹安徽省职业病防治研究所

平台石墨炉原子吸收法测定血中银常虹感光材料厂及其它工业每年使用大量的银。长期吸入银颗粒物和烟可引起银质沉着病，其表现为粘膜、眼睛和皮肤的色素沉着。医学上使用含银药品往往使银的吸入高于职业接触而引起毒副作用［１，２］。... 详细信息

平台石墨炉原子吸收法测定血中银常虹感光材料厂及其它工业每年使用大量的银。长期吸入银颗粒物和烟可引起银质沉着病，其表现为粘膜、眼睛和皮肤的色素沉着。医学上使用含银药品往往使银的吸入高于职业接触而引起毒副作用［１，２］。在职业医学和临床医学上，测定体液中...

关键词：血液检查血银平台石墨炉原子吸收法

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QTL alleles on chromosome 7 from fatty Meishan pigs reduce fat deposition

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Science China(Life Sciences) 2003年第1期46卷 10-17页

作者：岳根华 Petra Beeckmann Gerhard Moser Elisabeth Müller Hans Bartenschlager1 Stanislav Cepica Jaroslav Schrffel Antonin Stratil Hermann Geldermann Department of Animal Breeding and Biotechnology University of Hohenheim Institute of Animal Physiology and Genetics Academy of Sciences of the Czech Republic

For detecting QTL in the whole swine genome,1068 pigs from three F2 populations con-structed by crossing European Wild boar and Pietrain (W譖),Meishan and Pietrain (M譖),and Wild Boar and Meishan (W譓) were genotyped for genetic markers evenly spaced at approximately 20 cM inter-vals. A QTL analysis was performed using a least-squares method. Here the results of the QTL analysis on the porcine chromosome 7 are presented. QTL for carcass composition (*** weight,carcass length,backfat depth,abdominal fat and bacon meat) were mapped in the chromosomal region CYPA/CYPD-TNFB-S0102 in M譖 and W譓,but not in W譖. The QTL was additive. Surprisingly, in contrast to the parental phenotype, the QTL alleles from fatty Meishan were associated with thinner backfat than Pietrain and Wild Boar alleles,suggesting that the genome of the fatty Meishan pig contains genes which can reduce fat content of carcass substantially.

关键词： genomics, pig, SSC7, QTL, backfat.

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放疗所致肺部病变的影像学诊断

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放射学实践 1995年第4期10卷 168-170页

作者： scha.,CB 樊友武同济医科大学附属同济医院

放疗所致肺部病变的影像学诊断ＢｉｌｄｇｅｂｅｎｄｅＤｉａｇｎｏｓｔｉｋｐｕｌｍｏｎａｌｅｒＶｅｒｎｄｅｒｕｎｇｅｎｎａｃｈＳｔｒａｈｌｅｎｔｈｅｒａｐｉｅ￥Ｃ．Ｂ．Ｓｃｈｆｅｒ；Ｗ．Ｈｏｆｆｍａｎｎ；Ｍ．Ｓｃｈｉｅｂ... 详细信息

放疗所致肺部病变的影像学诊断ＢｉｌｄｇｅｂｅｎｄｅＤｉａｇｎｏｓｔｉｋｐｕｌｍｏｎａｌｅｒＶｅｒｎｄｅｒｕｎｇｅｎｎａｃｈＳｔｒａｈｌｅｎｔｈｅｒａｐｉｅ￥Ｃ．Ｂ．Ｓｃｈｆｅｒ；Ｗ．Ｈｏｆｆｍａｎｎ；Ｍ．Ｓｃｈｉｅｂｅ；Ｍ．Ｂａｍｂｅｒｇ；Ｃ．Ｄ．Ｃ...

关键词：放射疗法肺病变影像学诊断

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