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Journal of Integrative Plant Biology 2012年第1期54卷 15-32页

作者： Hamlet Avilés-Arnaut John Paul Délano-Frier Center of Research and Advanced studies (Cinvestav) at Irapuato:Unit for Plant Biotechnology and Genetic Engineering

Tomato systemin is a bioactive peptide that regulates the systemic activation of wound-responsive genes. It is released from its 200 amino acid precursor called prosystemin. Initial tissue-localization and hormone-induced expression assays indicated that the tomato prosystemin gene （sIPs） accumulates mainly in floral tissues and in response to exogenous abscisic acid and methyl jasmonate （MeJA） treatments, respectively. Later, the promoter regions of the Ps gene in tomato （solanum lycopersicum L. cv. Castlemart）, pepper （Capsicum annuum） and potato （solanum tuberosum） were isolated and an in silico analysis of the sIPs promoter revealed an over-representation of stress- and MeJA-responsive motifs. A subsequent 5＇ deletion analysis of the sIPs promoter fused to the/^-glucuronidase reporter （GUs） gene showed that the -221 to ＋40 bp proximal sIPs promoter region was sufficient to direct the stigma, vascular bundle-specific and MeJA-responsive expression of GUs in transgenic tobacco plants. Important ***-specific, light- and MeJA-responsive cis-elements were also present in this region. These findings provide relevant information regarding the transcriptional regulation mechanisms of the sIPs promoter operating in transgenic tobacco plants. They also suggest that its Ussue-specificity and inducible nature could have wide applicability in plant biotechnology.

关键词： cis-regulatory elements flower specific jasmonic acid methyl jasmonate promoter analysis prosystemin wound-responsive genes.

In silico Analysis of osr40c1 Promoter sequence Isolated from Indica Variety Pokkali

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Rice science 2017年第4期24卷 228-234页

作者： W.s.I.desILVA M.M.N.PERERA K.L.N.s.PERERA A.M.WICKRAMAsURIYA G.A.U.JAYAsEKERA Department of Plant sciences Faculty of ScienceUniversity of ColomboColombo 00300Sri Lanka Genetech Molecular Diagnostics and school of Gene Technology Colombo 00800Sri Lanka

The promoter region of a drought and abscisic acid （ABA） inducible gene, osr40c1, was isolated from a salt-tolerant indica rice variety Pokkali, which is 670 bp upstream of the putative translation start codon. In silico promoter analysis of resulted sequence showed that at least 15 types of putative motifs were distributed within the sequence, including two types of common promoter elements, TATA and CAAT boxes. Additionally, several putative cis-acing regulatory elements which may be involved in regulation of osr40c1 expression under different conditions were found in the 5′-upstream region of osr40c1. These are ABA-responsive element, light-responsive elements （ATCT-motif, Box I, G-box, GT1-motif, Gap-box and sp1）, myeloblastosis oncogene response element （CCAAT-box）, auxin responsive element （TGA-element）, gibberellin-responsive element （GARE-motif） and fungal-elicitor responsive elements （Box E and Box-W1）. A putative regulatory element, required for endosperm-specific pattern of gene expression designated as skn-1 motif, was also detected in the Pokkali osr40c1 promoter region. In conclusion, the bioinformatic analysis of osr40c1 promoter region isolated from indica rice variety Pokkali led to the identification of several important stress-responsive cis-acting regulatory elements, and therefore, the isolated promoter sequence could be employed in rice genetic transformation to mediate expression of abiotic stress induced genes.

关键词： osr40c1 promoter cis-acting regulatory element rice

Isotopic Composition of Atmospheric Precipitation in the cis-Ural Region

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Journal of Earth science 2022年第3期33卷 831-838页

作者： Alena s.Kazantseva Olga I.Kadebskaya Yuri V.Dublyansky Mining Institute of the Ural Branch of the Russian Academy of sciences PermRussia Institute of Geology University of InnsbruckInnsbruckAustria

Using the Global Network of Isotopes in Precipitation(GNIP)method at three stations in the cis-Ural region(Iskor,Perm,and Kungur),the monitoring results of the isotope composition of atmospheric precipitation conducted in 2016-2019 are *** lines of meteoric waters were obtained for each station and for the cis-Ural region as a *** latitudinal zoning of changes in isotope parameters from north to south in the cis-Ural region is noted,which is related to meteorological *** northern regions are characterized by isotopically light values compared to the southern ***,the correlation relationship between the isotopic compositions of atmospheric precipitation and meteorological parameters at each station is *** analysis data show that about 50%-90%of variations in the isotopic composition of precipitation within the annual cycle are determined by the air *** the same time,the amount of precipitation shows a relationship between the average strength and the isotopic composition of precipitation for a series of observations at Kungur station;for a series of observations at Iskor and Perm stations,there is no *** in the relationship with meteorological data are explained by different sources of moisture introduction to the station and,accordingly,the formation of the isotopic composition of *** flow of moisture is controlled by the transfer of air masses in different directions,the formation of cyclones and ***,for the northern part of the region during the year is characterized by the predominance of cyclones,whereas for the southern-anticyclones.

关键词： precipitation isotopic composition cis-Ural region correlation relationship spear-man’s correlation coefficient

Molecular mechanisms of heavy metal tolerance and evolution in invertebrates

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Insect science 2009年第1期16卷 3-18页

作者： Thierry K. s. Janssens Dick Roelofs Nico M. van straalen Department of Animal Ecology Institute of Ecological Science VU University Amsterdam The Netherlands

Following the genomics revolution, our knowledge of the molecular mechanisms underlying defenses against stress has been greatly expanded. Under strong selective pressure many animals may evolve an enhanced stress tolerance. This can be achieved by altering the structure of proteins （through mutations in the coding regions of genes） or by altering the amount of protein （through changes in transcriptional regulation）. The latter type of evolution can be achieved by substitutions in the promoter of the gene of interest （cisregulatory change） or by altering the structure or amount of transcriptional regulator proteins （trans-regulatory change）. The metallothionein system is one of the best studied stress response systems in the context of heavy metals. Metallothionein expression is assumed to be regulated by metal transcription factor 1 （MTF-1）; however, up to now the involvement of MTF- 1 has only been proven for some vertebrates and Drosophila. Data on invertebrates such as nematodes and earthworms suggest that other mechanisms of metallothionein induction may be present. A detailed study of Cd tolerance was done for a species of soilliving springtail, Orchesella cincta. The metallothionein gene of this species is overexpressed in metal-exposed field populations. Analysis of the metallothionein promoter has demon- strated extensive polymorphisms that have a fimctional significance, as shown in bioreporter assays. In a study comparing 20 different populations, the frequency of a high-expresser promoter allele was positively correlated with the concentration of metals in soil, especially Cd. The springtail study shows that cis-regulatory change of genes involved in the cellular stress response may contribute to evolution of metal tolerance.

关键词： Collembola evolution heavy metals metallothionein metal transcription factor 1 Orchesella cincta tolerance

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病态对象的鲁棒控制

化工学报 2004年第7期55卷 1131-1134页

作者：王伟 s.Engell 上海交通大学自控系上海200030 德国多特蒙德大学化工系多特蒙德44221

提出了一种适用于病态对象的鲁棒控制器设计过程 .该过程把增益修正法 (loopshapedesignprocedure)与频域响应近似法 (frequencyresponseapproximation)相结合 ,分两步设计二维自由度 (two degree of freedom)控制器 :即反馈控制器和前... 详细信息

提出了一种适用于病态对象的鲁棒控制器设计过程 .该过程把增益修正法 (loopshapedesignprocedure)与频域响应近似法 (frequencyresponseapproximation)相结合 ,分两步设计二维自由度 (two degree of freedom)控制器 :即反馈控制器和前置滤波器 .整个设计过程针对一个众所周知的病态系统控制问题进行演示 .

关键词：病态对象鲁棒控制 H∞控制两维自由度控制器

Forskolin and Phorbol 12-myristate 13-acetate modulates the expression pattern of AP-1 factors and cell cycle regulators in estrogen-responsive MCF-7 cells

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Genes & Diseases 2019年第2期6卷 159-166页

作者： R.L.Babu M.Naveen Kumar Rajeshwari H.Patil K.M.Kiran Kumar K.s.Devaraju Govindarajan T.Ramesh s.Chidananda sharma Department of Bioinformatics and Biotechnology Akkamahadevi Women’s UniversityJnanashakthi CampusVijayapura586108KarnatakaIndia Department of Microbiology and Biotechnology Bangalore UniversityJnana BharathiBengaluru560056KarnatakaIndia Department of Biochemistry Karnatak UniversityDharwad580003KarnatakaIndia Department of Biology Center for Biotechnology and Biomedical SciencesNorfolk State UniversityNorfolkVAUSA

Activator protein-1(AP-1)transcription factor is a key component of many signal transduction pathways involved in the regulation of cellular processes and controls rapid responses of mammalian cells when exposed to the variety of *** phorbol 12-myristate 13-acetate and Forskolin(Fo)are well-known kinase activators/stimulators of Protein Kinase C(PKC)and Protein Kinase A(PKA)***,these kinases are found to be present in transitional points of many cell signaling pathways,especially those involved in *** stimulating effect of PKC and PKA on the expression of AP-1 factors in MCF-7 breast cell proliferation is not well ***,the role of PKC by PMA treatment and the role of PKA by using Fo in MCF-7 cells is ***,cells treated with PMA showed increased cell proliferation,while Fo had no effect,but inhibited the PMA induced *** RT-PCR results showed the PMA induced c-Jun,c-Fos and fra-1 expressions compared to control and ***,Fo in combination with PMA,inhibit the PMA induced above mRNA expressions where Fo alone has no *** blot studies validated the c-Jun expressions in PMA treated MCF-7 ***,PMA increases the mRNA expression of Cyclin-E1,Cyclin-D1,and CDK-4,whereas Fo decreases their ***,mitogenic effect of PMA and inhibitory action of Fo on MCF-7 cells is probably enhanced via activation of AP-1 factors and concomitant action of cell cycle regulators in the downstream singling cascade.

关键词： AP-1 transcription factor Cell cycle Forskolin MCF-7 cells Phorbol esters

Non-oxygenic Photosynthesis in the sulphurous Lake cisó,North-eastern spain

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Acta Geologica sinica(English Edition) 2014年第S1期88卷 41-42页

作者： Angélica ANGLs LI Yiliang Department of Earth sciences The University of Hong Kong

1 Introduction situated in the Iberian Peninsula,Lake cisóis a sulphide-rich lake that belongs to the Ba?oles karstic system in Girona,north-eastern part of *** cisóis holomictic,and covers a small area of around 65... 详细信息

关键词： Lake cisó non-oxygenic photosynthesis sulphurous lake evolution microbial diversity

Activated Anilide in Heterocyclic synthesis： synthesis of New Dihydropyridines, Dihydropyridazines and Thiourea Derivatives

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Chinese Journal of Chemistry 2011年第6期29卷 1154-1162页

作者： Hafiz, Ibrahim s. A. Ramiz, Mahmoud M. M. sarhan, Ahmed A. M. Department of Chemistry Faculty of Education Suez Canal University Arish Egypt Faculty of Electronic Engineering Menofia University Menouf Egypt

A series of new dihydropyridines, butanamide, dihydropyridazines and thiourea derivatives have been prepared through the reactions of 3-aminopyridine （1） and N-（pyridin-3-yl）-3-（pyridin-3-ylimino）butanamide 3 with some electrophilic reagents, aryl diazonium salts and isothiocyanates. Elementary analysis, Ms, IR, and 1H NMR spectra confirmed the identity of the products.

关键词： lactones oxygen heterocycles cis-Wittig olefination mono and di-O-benzyl ethers cryptofolione stereoisomers

2,2′-二取代-1,1′-联萘化合物的NMR研究

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分析测试学报 1998年第1期17卷 9-13页

作者：钟世舟祝亚非许遵乐张小蘅中山大学测试中心中山大学化学系西蒙弗雷舍大学化学/生物化学系

用二维核磁共振谱（ＣＯＳＹ、ＨＭＱＣ、ＨＭＢＣ、ＦＧ＿ＨＭＢＣ）进一步研究了２，２′－二羟基－１，１′－联萘（１）、２，２′－二甲氧基－１，１′－联萘（２）、２，２′－亚甲二氧基－１，１′－联萘（３）、２，２′－亚乙... 详细信息

用二维核磁共振谱（ＣＯＳＹ、ＨＭＱＣ、ＨＭＢＣ、ＦＧ＿ＨＭＢＣ）进一步研究了２，２′－二羟基－１，１′－联萘（１）、２，２′－二甲氧基－１，１′－联萘（２）、２，２′－亚甲二氧基－１，１′－联萘（３）、２，２′－亚乙二氧基－１，１′－联萘（４）、２－羟基－２′－甲氧基－１，１′－联萘（５）、２－羟基－２′－乙氧基－１，１′－联萘（６）、２－羟基－２′－异丙氧基－１，１′－联萘（７）、２－羟基－２′－丁氧基－１，１′－联萘（８）和２－羟基－２′－氧乙酸基－１，１′－联萘（９）等９个２，２′－二取代－１，１′－联萘化合物，确定了它们的氢谱和碳谱谱峰的归属，指出联萘化合物的立体结构、萘环的磁各向异性对质子谱有明显的影响，特别是Ｈ８（Ｈ８′）；而其对环碳的影响可以忽略。

关键词：二维核磁共振谱联萘衍生物 NMR

Identification of ta-siRNAs and cis-nat-siRNAs in Cassava and Their Roles in Response to Cassava Bacterial Blight

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Genomics, Proteomics & Bioinformatics 2013年第3期11卷 172-181页

作者： Andrés Quintero Alvaro L. Pérez-Quintero Camilo López Departamento de Biología Universidad Nacional de Colombia

Trans-acting small interfering RNAs （ta-siRNAs） and natural cis-antisense siRNAs （cisnat-siRNAs） are recently discovered small RNAs （sRNAs） involved in post-transcriptional gene silencing, ta-siRNAs are transcribed from genomic loci and require processing by microRNAs （miRNAs）. cis-nat-siRNAs are derived from antisense RNAs produced by the simultaneous tran- scription of overlapping antisense genes. Their roles in many plant processes, including pathogen response, are mostly unknown. In this work, we employed a bioinformatic approach to identify ta-siRNAs and cis-nat-siRNAs in cassava from two sRNA libraries, one constructed from healthy cassava plants and one from plants inoculated with the bacterium Xanthomonas axonopodis pv. manihotis （Xam）. A total of 54 possible ta-siRNA loci were identified in cassava, including a homo- log of TAs3, the best studied plant ta-siRNA. Fifteen of these loci were induced, while 39 were repressed in response to Xam infection. In addition, 15 possible c/s-natural antisense transcript （cis-NAT） loci producing siRNAs were identified from overlapping antisense regions in the genome, and were found to be differentially expressed upon Xam infection. Roles of sRNAs were predicted by sequence complementarity and our results showed that many sRNAs identified in this work might be directed against various transcription factors. This work represents a significant step toward understanding the roles of sRNAs in the immune response of cassava.

关键词： ta-siRNA nat-siRNA Natural antisense transcriptCassava Xanthomonas