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Structural Characterization of Chloroplast Genome in Alpinia japonica(Thunb.)Miq.,a Medicinal Plant of the Genus Alpinia

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Phyton-International Journal of Experimental Botany 2024年第8期93卷 1897-1911页

作者： Wentao Sheng xi Lei xinjie Chen Quan Kuang Department of Biological Technology Nanchang Normal UniversityNanchang330032China

The analysis of chloroplast gene characteristics in Alpinia japonica(Thunb.)*** of great significance for developing relevant genetic *** high-throughput sequencing and bioinformatic research were performed to analyze the chloroplast genome characteristics of *** total chloroplast genome length of *** was 161,906 bp,with a typical circular tetrameric *** 133 genes were annotated,comprising 87 protein-coding,38 tRNA,and 8 rRNA ***,22 genes contained two copies,and 18 genes owned *** sequence analysis showed that it contains 321 simple sequence repeats(SSRs)and 37 long segment *** with the chloroplast genomes of eight representative plants in the genus Alpinia,the gene structure,type,and quantity were relatively ***12 was the highest variation site in the entire chloroplast gene.A phylogenetic tree showed that the genus Alpinia was the most closely related to the genus ***,*** is the most closely related to Alpinia chinensis belonging to the genus ***,the chloroplast genome of a new species was reported in the genus Alpinia,and a basis was provided for the utilization of Alpinia plants as a medical resource.

关键词： The genus Alpinia Alpinia japonica(Thunb.)Miq. chloroplast genome phylogenetic tree

Regulation of Toll-like receptor signaling in innate immunity

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Science china.Life Sciences) 2010年第1期53卷 34-43页

作者： AN HuaZhang,QIAN Cheng & CAO XueTao National Key Laboratory of medical Immunology & Institute of Immunology,Second military medical university,Shanghai 200433,china National Key Laboratory of medical Immunology & Institute of Immunology Second Military Medical University Shanghai China

Toll-like receptors sense invading pathogens by recognizing a wide variety of conserved pathogen-associated molecular patterns(PAMPs).The members of the TLR family selectively utilize adaptor proteins MyD88,TRIF,TIRAP and TRAM to activate overlapping but distinct signal transduction pathways which trigger production of different panels of mediators such as proinflammatory cytokines and type I *** mediators not only control innate immunity but also direct subsequently developed adaptive *** activation is strictly and finely regulated at multiple levels of the signal transduction pathways.

关键词： Toll-like receptors TLR signaling

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微生物表面呈现技术及应用

生物化学与生物物理进展 1997年第5期24卷 422-426页

作者：王雨田胡家露陈苏民杨胜利第四军医大学全军消化疾病研究所西安710033 第四军医大学生物化学教研室西安710032 中国科学院上海生物工程研究中心上海200233

微生物表面呈现技术以其广泛的应用前景 ,日益引起关注 .

关键词：微生物表面呈现方法技术

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Stable Skin-specific Overexpression of Human CTLA4-Ig in Transgenic Mice through Seven Generations

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Acta Biochimica et Biophysica Sinica 2006年第3期38卷 171-178页

作者： Yong WANG~1 Yong NI~(1,3) Hong WEI~1* Feng-Chao WANG~2 Liang-Peng GE~1 xiang GAO~41 Department of Animal Science,College of Basic Medicine,Third military medical university,Chongqing 400038,china.2 Institute of Combined Injury,College of military Preventive Medicine,Third military medical university,Chongqing 400038,china.3 School of Life Sciences,Southwest university,Chongqing 400715,china.4 Animal Model Research Institute,Nanjing university,Nanjing 210093,china Department of Animal Science College of Basic Medicine Third Military Medical UniversityChongqing 400038 China School of Life Sciences Southwest UniversityChongqing 400715 China Institute of Combined Injury College of Military Preventive Medicine Third Military Medical UniversityChongqing 400038 China Animal Model Research Institute Nanjing UniversityNanjing 210093 China

Skin graft rejection is a typical cellular immune response,mainly mediated by T *** lymphocyte associated antigen 4-immunoglobin (CTLA4-Ig) extends graft survival by blocking the T cellco-stimulation pathway and inhibiting T cell *** investigate the efficacy of CTLA4-Ig in prolong-ing skin graft survival,human CTLA4-Ig (hCTLA4-Ig) was engineered to overexpress in mouse skin bytransgenesis using the K14 *** transcription-polymerase chain reaction (RT-PCR) and West-ern blot assay indicated that the expression of CTLA4-Ig remained skin-specific and relatively constantcompared to the internal control protein,AKT,through seven *** presence and concentrationof the hCTLA4-Ig protein in transgenic mouse sera was determined by enzyme-linked immunosorbent assay(ELISA),and the results indicated that the serum CTLA4-Ig concentration also remained constant *** of transgenic mouse skins grafted onto rat wounds was remarkably prolonged com-pared to that of wild-type skins from the same mouse strain,and remained comparable among all *** suggested that the bioactive hCTLA4-Ig protein was stably expressed in transgenical micethrough at least seven generations,which was consistent with the stable skin-specific CTLA4-Ig *** results demonstrated that the transgenic expression of hCTLA4-Ig in skin driven by the K14 promoterremained constant through generations,and a transgenic line can be established to provide transgenic skinwith extended survival reproducibly.

关键词： cytotoxic T lymphocyte antigen 4-Ig transgenic mice skin K14 promoter

Benchmarking Algorithms for Gene Set Scoring of Single-cell ATAC-seq Data

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Genomics, Proteomics & Bioinformatics 2024年第2期22卷 27-42页

作者： xi Wang Qiwei Lian Haoyu Dong Shuo Xu Yaru Su xiaohui Wu Pasteurien College Suzhou Medical College of Soochow UniversitySoochow UniversitySuzhou 215000China Department of Automation Xiamen UniversityXiamen 361005China College of Mathematics and Computer Science Fuzhou UniversityFuzhou 350116China

Gene set scoring(GSS)has been routinely conducted for gene expression analysis of bulk or single-cell RNA sequencing(RNA-seq)data,which helps to decipher single-cell heterogeneity and cell type-specific variability by incorporating prior knowledge from functional gene ***-cell assay for transposase accessible chromatin using sequencing(scATAC-seq)is a powerful technique for interrogating single-cell chromatin-based gene regulation,and genes or gene sets with dynamic regulatory potentials can be regarded as cell type-specific markers as if in single-cell RNA-seq(scRNA-seq).However,there are few GSS tools specifically designed for scATAC-seq,and the applicability and performance of RNA-seq GSS tools on scATAC-seq data remain to be ***,we systematically benchmarked ten GSS tools,including four bulk RNA-seq tools,five scRNA-seq tools,and one scATAC-seq ***,using matched scATAC-seq and scRNA-seq datasets,we found that the performance of GSS tools on scATAC-seq data was comparable to that on scRNA-seq,suggesting their applicability to ***,the performance of different GSS tools was extensively evaluated using up to ten scATAC-seq ***,we evaluated the impact of gene activity conversion,dropout imputation,and gene set collections on the results of *** show that dropout imputation can significantly promote the performance of almost all GSS tools,while the impact of gene activity conversion methods or gene set collections on GSS performance is more dependent on GSS tools or ***,we provided practical guidelines for choosing appropriate preprocessing methods and GSS tools in different application scenarios.

关键词： Single-cell ATAC-seq Gene set scoring Pathway analysis Single-cell RNA-seq Benchmark

Multiple pretreatments can effectively improve the functionality of mesenchymal stem cells

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World Journal of Stem Cells 2024年第2期16卷 58-63页

作者： xin-xing Wan xi-Min Hu Kun xiong Department of Endocrinology Third Xiangya HospitalCentral South UniversityChangsha 410013Hunan ProvinceChina Department of Dermatology Xiangya HospitalCentral South UniversityChangsha 410008Hunan ProvinceChina Department of Anatomy and Neurobiology School of Basic Medical ScienceCentral South UniversityChangsha 410013Hunan ProvinceChina

In this editorial,we offer our perspective on the groundbreaking study entitled“Hypoxia and inflammatory factor preconditioning enhances the immunosup-pressive properties of human umbilical cord mesenchymal stem cells”,recently published in World Journal of Stem *** over three decades of research on the clinical application of mesenchymal stem cells(MSCs),only a few therapeutic products have made it to clinical use,due to multiple preclinical and clinical challenges yet to be *** study proved the hypoxia and inflammatory factor preconditioning led to higher immunosuppressive effects of MSCs without damaging their biological characteristics,which revealed the combination of inflammatory factors and hypoxic preconditioning offers a promising approach to enhance the function of *** we delve deeper into the intricacies of pretreat-ment methodologies,we anticipate a transformative shift in the landscape of MSC-based therapies,ultimately contributing to improved patient outcomes and advancing the field as a whole.

关键词： Mesenchymal stem cells Inflammatory factor Hypoxia Pretreatment

Distribution of nitric oxide synthase in stomach myenteric plexus of rats

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World Journal of Gastroenterology 2001年第6期7卷 852-854页

作者： xi Peng Jin-Bin Feng Hong Yan Yun Zhao Shi-Liang Wang Institute of Burn Research,Southwest Hospital,Third military medical university,Chongqing 400038,china Institute of Burn Research Southwestern Hospital Third Military Medical University Chongqing 400038 China. pxlr@***

AIM: To study the distribution of nitric oxide synthase (NOS) in rat stomach myenteric plexus. METHODS: The distribution of NOS in gastric wall was studied in quantity and location by the NADPH-diaphorase (NDP) histochemical staining method and whole mount preparation technique. RESULTS: NOS was distributed in whole stomach wall, most of them were located in myenteric plexus, and distributed in submucosal *** shape of NOS positive neurons was basically similar, most of them being round and oval in shape. But their density, size and staining intensity varied greatly in the different parts of stomach. The density was 62+/-38 cells mm(2) (antrum), 43+/-32 cells/mm(2) (body), and 32+/-28 cells mm(2) (fundus), respectively. The size and staining intensity of NOS positive neurons in the fundus were basically the same, the neurons being large and dark stained, while they were obviously different in antrum. In the body of the stomach, the NOS positive neurons were in an intermediate state from fundus to antrum. There were some beadlike structures which were strung together by NOS positive varicosities in nerve fibers, some were closely adherent to the outer walls of blood vessels. CONCLUSION: Nitric oxide might be involved in the modulation of motility, secretion and blood circulation of the stomach, and the significant difference of NOS positive neurons in different parts of stomach myenteric plexus may be related to the physiologic function of stomach.

关键词： Animals Female Myenteric Plexus Nitric Oxide Synthase Rats Rats, Wistar Stomach Tissue Distribution

Adenovirus-expressed preS2 antibody inhibits hepatitis B virus infection and hepatic carcinogenesis

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World Journal of Gastroenterology 2012年第4期18卷 349-355页

作者： Qian Zhang,Department of Infectious Diseases,Changhai Hospital,Second military medical university,Shanghai 200433,china.Zhi-Qing Li,Hu Liu,Jia-He Yang,Department of Hepatic Surgery,Eastern Hepatobiliary Surgery Hospital,Second military medical university,Shanghai 200438,china Qian Zhang Department of Infectious Diseases Changhai Hospital Second Military Medical University Shanghai 200433 China.

AIM:To investigate the inhibitory effect of hepatitis B virus (HBV) preS2 antibody (preS2Ab) against HBV in-fection and HBV-associated hepatic ***:An adenoviral vector carrying the full-length light and heavy chains of the HBV preS2Ab gene,Ad315-preS2Ab,was *** linked immunosorbent assay (ELISA) and Western blotting analyses were used to determine the preS2Ab expres-sion levels in *** techniques were used to examine the binding affinity between the expressed HBV preS2Ab and HBV-positive liver *** were also used to determine hepatitis B surface antigen (HBsAg) levels to assess the inhibitory effect of the preS2Ab against HBV infection in L02 *** inhibitory effect of preS2Ab against hepatic carcinogen-esis was studied with diethylnitrosamine (DEN)-induced hepatocellular carcinomas (HCCs) in HBV transgenic ***:The expression of HBV preS2Ab increased with increases in the multiplicity of infection (MOI) of Ad315-preS2Ab in L02 cells,with 350.87 ± 17.37 μg/L of preS2Ab when the MOI was 100 plaque forming units (pfu)/*** expressed preS2Abs could recog-nize liver cells from HBV transgenic *** results showed that L02 cells expressing preS2Ab produced less HBsAg after treatment with the serum of HBV pa-tients than parental L02 cells expressing no *** transgenic mice treated with Ad315-preS2Ab had fewer and smaller cancerous nodes after induction with DEN than mice treated with a blank Ad315 vec-tor or untreated ***,the administration of Ad315-preS2Ab could alleviate hepatic cirrhosis and decrease the serum levels of alanine transaminase and aspartate ***:Adenovirus-mediated HBV preS2Ab expression could inhibit HBV infection in L02 cells,and then inhibit DEN-induced hepatocellular carcinogenesis and protect hepatic function in HBV transgenic mice.

关键词： Hepatitis B virus Adenoviral vector PreS2 antibody Hepatocellular carcinoma Gene therapy

Histological changes following surgically-assisted rapid tooth movement through resistance reduction and distraction osteogenesis in dogs

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Journal of Pharmaceutical Analysis 2010年第2期22卷 139-142页

作者： xi Chen1,Jian-Lin Liu2,Juan Dai3,Shuang Wang4,Ya-Li Huang11. Department of Stomatology,the First Affiliated Hospital,medical School of xi’an Jiaotong university,xi’an 710061 2. Department of Stomatology,No.2 Traditional Chinese medical Hospital,Shenzhen 518034 3. Department of Orthodontics,School of Stomatology,fourth military medical university,xi’an 710032 4. Department of Orthodontics,Stomatological Hospital of xi’an Jiaotong university,xi’an 710004,china. Department of Stomatology First Affiliated Hospital Medical School of Xi'an Jiaotong University Xi'an 710061 China Department of Stomatology No. 2 Traditional Chinese Medical Hospital Shenzhen 518034 China Department of Orthodontics School of Stomatology Fourth Military Medical University Xi'an 710032 China Department of Orthodontics Stomatological Hospital of Xi'an Jiaotong University Xi'an 710004 China

Objective To investigate the histological changes of rapid tooth movement in dogs treated by resistance reduction and distraction osteogenesis,aiming to establish an animal model and further to reveal the remodeling mechanism of rapid tooth movement. Methods A total of 8 local hybrid dogs were selected as subjects for this study. The second pre-molar was extracted on both sides. The experimental side underwent alvelor surgery for resistance reduction and a home-made tooth-borne intraoral distraction device was installed for rapid tooth movement,while for the other side (control side) only tooth-borne intraoral distraction device was used for rapid tooth movement. The longest active force-delivery span was 2 weeks,followed by 6-week retention. The distance between the moved tooth and anchor unit was recorded weekly,and radiography was performed for each side before and after distraction. The surrounding tissues including periodontal ligament and alveolar bone were sectioned for histological analysis. Results The average distance of tooth movement was 3.55mm on the experimental side and 1.11mm on the control side. The rate of tooth movement was notably higher (P<0.01) and no significant apical root resorption was detected by X-ray on the experimental side. The active alvelor bone remodeling was found on the tension and pressure sides. However,there was no significant difference between the experimental side and the control side after the retention period. Conclusion The rate of orthodontic tooth movement can be accelerated through resistance reduction and periodontal distraction without any unfavorable effects but at minimal anchorage loss.

关键词： periodontal ligament distraction osteogenesis resistance reduction rapid tooth movement

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