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Factors that contribute to the mycoparasitism stimulus in Trichoderma atroviride. strain P1

浙江大学学报（农业与生命科学版） 2004年第4期30卷 421-421页

作者： Woo S L formisano e Fogliano V Cosenza C Mauro A Turrà D Soriente I Ferraioli S Scala F Lorito M Dip. Arboricoltura Botanica e Patologia Vegetale-Sez. Patologia Vegetale Lab. di Lotta Biologica and Dip. Di Scienza degli Alimenti Università di Napoli "Federico II" Via Università 100 80055 Portici (NA) Italy. Dip. Arboricoltura Botanica e Patologia Vegetale-Sez. Patologia Vegetale Lab. di Lotta Biologica and Dip. Di Scienza degli Alimenti Università di Napoli "Federico II" Via Università 100 80055 Portici (NA) Italy.Dip. Arboricoltura Botanica e Patologia Vegetale-Sez. Patologia Vegetale Lab. di Lotta Biologica and Dip. Di Scienza degli Alimenti Università di Napoli "Federico II" Via Università 100 80055 Portici (NA) Italy.Dip. Arboricoltura Botanica e Patologia Vegetale-Sez. Patologia Vegetale Lab. di Lotta Biologica and Dip. Di Scienza degli Alimenti Università di Napoli "Federico II" Via Università 100 80055 Portici (NA) Italy. Dip. Arboricoltura Botanica e Patologia Vegetale-Sez. Patologia Vegetale Lab. di Lotta Biologica and Dip. Di Scienza degli Alimenti Università di Napoli "Federico II" Via Università 100 80055 Portici (NA) Italy. Dip. Arboricoltura Botanica e Patologia Vegetale-Sez. Patologia Vegetale Lab. di Lotta Biologica and Dip. Di Scienza degli Alimenti Università di Napoli "Federico II" Via Università 100 80055 Portici (NA) Italy. Dip. Arboricoltura Botanica e Patologia Vegetale-Sez. Patologia Vegetale Lab. di Lotta Biologica and Dip. Di Scienza degli Alimenti Università di Napoli "Federico II" Via Università 100 80055 Portici (NA) Italy. Dip. Arboricoltura Botanica e Patologia Vegetale-Sez. Patologia Vegetale Lab. di Lotta Biologica and Dip. Di Scienza degli Alimenti Università di Napoli "Federico II" Via Università 100 80055 Portici (NA) Italy. Dip. Arboricoltura Botanica e Patologia Vegetale-Sez. Patologia Vegetale Lab. di Lotta Biologica and Dip. Di Scienza degli Alimenti Università di Napoli "Federico II" Via Università 100 80055 Portici (NA) Italy.

Trichoderma atroviride strain P1 has been used extensively to study the mycoparasitic mechanisms in the interaction between plant pathogenic host and beneficial antagonistic fungi. Mutants of P1 containing the green fluorescent protein (gfp) or glucose oxidase (gox) reporter systems and different inducible promoters (from the exochitinase nag1 gene, or the endochitinase ech42 gene of P1) were used to determine the factors that activate the biocontrol gene expression cascade in the antagonist. The following compounds were tested singly and in various combinations: purified Trichoderma P1 enzymes (endochitinase, exochitinase, chitobiosidase, glucanase); antagonist culture filtrates (T. atroviride P1 wild-type and relative knock-out mutants, T. harzianum, T. reesei); pathogen culture filtrates (Botrytis, Pythium, Rhizoctonia); purified fungal cell walls (CWs) from Trichoderma, Botrytis, Pythium, Rhizoctonia; colloidal crab shell chitin; and plant extracts from cucumber leaves, stems or roots. Strong induction of mycoparasitism was found with the various digestion products produced by treating fungal CWs and colloidal chitin with purified enzymes or fungal culture filtrates. Filtrates from chitinase knock-out mutants, as well as CWs from Oomycetes fungi, were less active in producing the stimulus for mycoparasitism. The host CW digestion products were separated by molecular weight (MW) to determine which compounds were able to activate Trichoderma. Micromolecules of MW less than 3 kDa were found to trigger mycoparasitism gene expression before physical contact with the host pathogen. These compounds stimulated mycelial growth and spore germination of the antagonist. Purification of these host-derived compounds was conducted by HPLC and in vivo assay. The obtained inducers were able to stimulate both the production of endochitinase and exochitinase enzymes, even under repressing conditions in the presence of glucose. Inducers stimulated the biocontrol effect of P1 in the

关键词：真菌寄生现象刺激因子木霉属真菌菌株

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The Trichoderma-plant interaction is mediated by avirulence proteins produced by this fungus

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浙江大学学报（农业与生命科学版） 2004年第4期30卷 422-422页

作者： Ruocco M Lanzuise S Woo S L Ambrosino P Marra R Turrà D Gigante S formisano e Scala F Kip N P J G M de Wit Lorito M Dip. Arboricoltura Botanica e Patologia Vegetale-Sez. Patologia Vegetale Lab. di Lotta Biologica Università di Napoli "Federico II" and CNR-IPP Via Università 100 Portici (NA) 80055 Italy Dip. Arboricoltura Botanica e Patologia Vegetale-Sez. Patologia Vegetale Lab. di Lotta Biologica Università di Napoli "Federico II" and CNR-IPP Via Università 100 Portici (NA) 80055 Italy Dip. Arboricoltura Botanica e Patologia Vegetale-Sez. Patologia Vegetale Lab. di Lotta Biologica Università di Napoli "Federico II" and CNR-IPP Via Università 100 Portici (NA) 80055 Italy Dip. Arboricoltura Botanica e Patologia Vegetale-Sez. Patologia Vegetale Lab. di Lotta Biologica Università di Napoli "Federico II" and CNR-IPP Via Università 100 Portici (NA) 80055 ItalyDip. Arboricoltura Botanica e Patologia Vegetale-Sez. Patologia Vegetale Lab. di Lotta Biologica Università di Napoli "Federico II" and CNR-IPP Via Università 100 Portici (NA) 80055 Italy Dip. Arboricoltura Botanica e Patologia Vegetale-Sez. Patologia Vegetale Lab. di Lotta Biologica Università di Napoli "Federico II" and CNR-IPP Via Università 100 Portici (NA) 80055 Italy Dip. Arboricoltura Botanica e Patologia Vegetale-Sez. Patologia Vegetale Lab. di Lotta Biologica Università di Napoli "Federico II" and CNR-IPP Via Università 100 Portici (NA) 80055 Italy Dip. Arboricoltura Botanica e Patologia Vegetale-Sez. Patologia Vegetale Lab. di Lotta Biologica Università di Napoli "Federico II" and CNR-IPP Via Università 100 Portici (NA) 80055 Italy Dip. Arboricoltura Botanica e Patologia Vegetale-Sez. Patologia Vegetale Lab. di Lotta Biologica Università di Napoli "Federico II" and CNR-IPP Via Università 100 Portici (NA) 80055 Italy Dip. Arboricoltura Botanica e Patologia Vegetale-Sez. Patologia Vegetale Lab. di Lotta Biologica Università di Napoli "Federico II" and CNR-IPP Via Università 100 Portici (NA) 80055 Italy Dip. Arboricoltura Botanica e Patologia Vegetale-Sez. Patologia Vegetale Lab. di Lotta Biologica

The molecular basis of Trichoderma -plant interaction is very complex and still not completely understood. The colonization of the root system by rhizosphere competent strains of Trichoderma results in increased development of root/aerial systems, in improved yields and in plant disease control. Other beneficial effects, such as the induction of plant systemic resistance, have also been described. To understand the mechanisms involved we are using different approaches, including the making of transformants expressing genes that encode for compounds able to affect plant response to pathogens. Trichoderma transformants carrying the avirulence gene Avr4 from Cladosporium fulvum under the control of constitutive and inducible promoters were obtained and tested on tomato plants having the Cf4 resistance gene. Necrosis and suberification zones, similar to the symptoms appearing during Cladosporium-tomato interaction, were found when the roots of the Cf4 plants were treated with Avr4-Trichoderma. This demonstrates that selected Trichoderma strains are able to transfer to the plant molecules that may deeply affect metabolism, disease resistance etc. Therefore, these beneficial fungi can be regarded as biotechnological tools to provide a variety of crops with useful compounds. Moreover, in in vitro competition assays the transformants were found to be more effective as antagonists against Alternaria alternata than the wild type. Trichoderma sends a variety of biochemical signals to the plants including avirulence molecules; therefore the presence of avr-like proteins in the fungus proteome was investigated. Proteome analysis has permitted us to isolate and sequence many proteins potentially having this function. From the extracellular protein extracts, we have purified and sequenced a protein with structural characteristics similar to Avr4 of C. fulvum. The protein, Hytra1, was found to be a hydrophobin with chitin binding activity, the typical 8 cysteine residues, and 4 dis

关键词：无毒力蛋白质真菌木霉属生产植物

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Non-alcoholic fatty liver disease:Dietary and nutraceutical approaches

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Liver Research 2023年第3期7卷 216-227页

作者： Ludovica Cogorno elena formisano Andrea Vignati Amalia Prigione Antonio Tramacere Consuelo Borgarelli Samir Giuseppe Sukkar Livia Pisciotta Department of experimental Medicine-Medical Pathophysiology Food Science and Endocrinology SectionSapienza University of RomeRomeItaly Department of Internal Medicine University of GenoaGenoaItaly Dietetics and Clinical Nutrition Unit IRCCS Policlinic Hospital San MartinoGenoaItaly

Non-alcoholic fatty liver disease(NAFLD),defined as the presence of fat accumulation in imaging or histology in more than 5%of hepatocytes and exclusion of other causes for secondary hepatic fat accumulation,is one of the major causes of chronic liver disease *** syndrome is associated with an increased risk of progression from NAFLD to non-alcoholic steatohepatitis(NASH),fibrosis,and forthcoming liver ***,genetic predisposition contributes to the risk of NAFLD *** review explores the role of diets and nutraceuticals in delaying the development and the evolution of NAFLD to chronic liver *** Mediterranean diet,high-protein diet,lowcarbohydrate/high-fat diet,high-carbohydrate/low-fat diet,and intermittent fasting are the dietary approaches investigated given the presence of relevant literature ***,this review focused on nutraceuticals with proven efficacy in ameliorating NAFLD and grouped them into four different categories:plant-based nutraceuticals(Ascophyllum nodosum and Fucus vesiculosus,Silymarin,Berberine,Curcumin,Resveratrol,Nigella sativa,Quercetin),vitamin-like substances(vitamin e,vitamin D,vitamin C,coenzyme Q10,inositol),fatty acids(omega-3),and microbiota-management tools(probiotics).

关键词： Non-alcoholic fatty liver disease(NAFLD) Metabolic-associated fatty liver disease(MAFLD) Non-alcoholic steatohepatitis(NASH) Non-alcoholic fatty liver(NAFL) Diets Nutraceuticals

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efficacy of a therapeutic strategy for eradication of Helicobacter pylori infection

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World Journal of Gastroenterology 2012年第33期18卷 4542-4548页

作者： Giuliana Sereni Francesco Azzolini Lorenzo Camellini Debora formisano Francesco Decembrino Veronica Iori Cristiana Tioli Maurizio Cavina Francesco Di Mario Giuliano Bedogni Romano Sassatelli Unit of Gastroenterology and Digestive endoscopy Arcispedale Santa Maria Nuova Istituto di Ricovero e Cura a Carattere Scientifico 42123 Reggio Emilia Italy Statistics and Clinical epidemiology Unit Arcispedale Santa Maria Nuova Istituto di Ricovero e Cura a Carattere Scientifico 42123 Reggio Emilia Italy Unit of Gastroenterology Ospedale S. Maria di Ca' Foncello 31100 Treviso Italy

AIM: To determine the efficacy of our therapeutic strategy for Helicobacter pylori (H. pylori) eradication and to identify predictive factors for successful eradication. MeTHODS: From April 2006 to June 2010, we retrospectively assessed 2428 consecutive patients (1025 men, 1403 women; mean age 55 years, age range 18-92 years) with gastric histology positive for H. pylori infection referred to our unit for 13-C urea breath test(UBT), after first-line therapy with proton pump inhibitor (PPI) b.i.d. + amoxicillin 1 g b.i.d. + clarithromycin 500 mg b.i.d. for 7 d. Patients who were still positive to UBT were recommended a second-line therapy (PPI b.i.d. + amoxicillin 1 g b.i.d. + tinidazole 500 mg b.i.d. for 14 d). Third choice treatment was empirical with PPI b.i.d. + amoxicillin 1 g b.i.d. + levofloxacin 250 mg b.i.d. for 14 d. ReSULTS: Out of 614 patients, still H. pylori-positive after first-line therapy, only 326 and 19 patients respectively rechecked their H. pylori status by UBT after the suggested second and third-line regimens. "Per protocol" eradication rates for first, second and thirdline therapy were 74.7% (95% CI: 72.7%-76.4%), 85.3% (95% CI: 81.1%-89.1%) and 89.5% (95% CI: 74.9%-103%) respectively. The overall percentage of patients with H. pylori eradicated after two treatments was 97.8% (95% CI: 97.1%-98.4%), vs 99.9% (95% CI: 99.8%-100%) after three treatments. The study found that eradication therapy was most effective in patients with ulcer disease (P especially in those with duodenal ulcer. Smoking habits did not significantly affect the eradication rate. CONCLUSION: First-line therapy with amoxicillin and clarithromycin produces an H. pylori eradication rate comparable or superior to other studies and secondline treatment can still be triple therapy with amoxicillin and tinidazole.

关键词：幽门螺旋杆菌治疗方案感染情况根除疗效阿莫西林质子泵抑制剂平均年龄

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Assessment of Physical and Mechanical Properties of Concrete Produced from Various Portland Cement Brands

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Open Journal of Composite Materials 2019年第4期9卷 327-337页

作者： Asal Soltani Salim Khoso Manthar Ali Keerio Antonio formisano Department of Civil and environmental engineering The University of Toledo Toledo Ohio USA Department of Civil engineering Quaid-e-Awam University College of Engineering Sciences & Technology (QUCEST) Larkana Sindh Pakistan Department of Structures for engineering and Architecture School of Polytechnic and Basic Sciences University of Naples “Federico II” Naples Italy

Construction projects require cement, which must have definite qualities so as to play its part separately in structure. The compressive strength of cement and, consequently, its physical properties could vary as a result of variations in the raw materials, as well as in manufacturing conditions. Therefore, an experimental study has been checked out herein by investigating the mechanical and physical properties of concrete specimens by means of four different types of Ordinary Portland Cements, namely Black Bull, Lucky, Pak Land and DG obtained from the market of Sindh Pakistan. Based on obtained results, it was found that Lucky cement type performed well when compared to other three types both in compressive and tensile strength of concrete. Although there were some differences in the physical properties of cement used in this study, however, they all meet with the requirements of international standards.

关键词： Cement Brands Concrete Compressive Strength Tensile Strength

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CD28胞内域对初始T细胞CD28受体与配体结合具有关键调控作用

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Science Bulletin 2021年第2期66卷 107-110,M0003页

作者：钱呈睿夏凡 Anne-Marie Sartre Anthony formisano Sébastian Jaeger Jacques A.Nunès 郭晓君何海涛 Aix Marseille Univ CNRSINSERMCIML13288 MarseilleFrance Shanghai Blood Center Shanghai 200051China Department of Radiation Oncology Fudan University Shanghai Cancer CenterShanghai 200032China Department of Oncology Shanghai Medical CollegeFudan UniversityShanghai 200032China Aix Marseille Univ CNRSINSERMInstitut Paoli-CalmettesCRCM13273 MarseilleFrance Centre de Recherche en Cancérologie de Marseille Immunology and CancerEquipe Labellisée Fondation pour la Recherche Médicale13273 MarseilleFrance

初始T细胞活化是由T细胞受体(TCR)及共刺激分子与其配体结合引起的,其中CD28共刺激信号在促进初始T细胞的活化过程中起关键作用.本文研究了小鼠CD4^(+)T细胞中CD28与B7配体的结合活性在CD28介导的信号通路激活中的调控机制.结果发现虽... 详细信息

初始T细胞活化是由T细胞受体(TCR)及共刺激分子与其配体结合引起的,其中CD28共刺激信号在促进初始T细胞的活化过程中起关键作用.本文研究了小鼠CD4^(+)T细胞中CD28与B7配体的结合活性在CD28介导的信号通路激活中的调控机制.结果发现虽然初始CD4^(+)T细胞表面CD28-B7的本底结合力很低,但当同步给予TCR刺激可触发CD28胞内域激酶SRC酪氨酸磷酸化,并由此显著增强CD28-B7的结合力.此外通过CD28胞内域缺失的小鼠模型,进一步验证CD28胞内结构域的存在是TCR刺激信号增强CD28-B7结合力的必要条件.据此,本研究证实CD28胞内域在其配体结合过程中承担了关键性的调控作用,并为TCR和CD28相互作用介导初始T细胞活化提供了新的证据.

关键词：初始T细胞 CD28 共刺激信号酪氨酸磷酸化 T细胞活化胞内域共刺激分子活化过程

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everolimus plus long-acting somatostatin analogs in thymic epithelial malignancies

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World Journal of Clinical Oncology 2012年第7期3卷 111-115页

作者： Giovannella Palmieri Carlo Buonerba Piera Federico Luigi formisano Lucia Nappi Giuseppe Di Lorenzo Mirella Marino Vincenzo Damiano Dipartimento di endocrinologia ed Oncologia Clinica e Molecolare Università Federico Ⅱ 80131 Napoli Italy Centro Regionale Tumori Rari (Regional Center for Rare Tumors) University Federico Ⅱ 80128 Naples Italy Department of Pathology Regina Elena National Cancer Institute 00144 Rome Italy

Although thymic epithelial tumors(TeTs) are rare in the general population, they represent the most frequently diagnosed primary malignant tumor of the anterior mediastinum. Unlike localized disease, metastatic disease is invariably fatal. While several chemotherapy agents have proven to be effective in TeTs, somatostatin analogs are the only targeted agents with an established role in this disease. everolimus is an m TOR inhibitor with multiple application in oncology. In this report, we show for the first time that everolimus was effective in two heavily pretreated patients with advanced TeTs, with a progression-free survival longer than 1 year and minimal toxicity.

关键词： Thymoma everolimus mTOR Mediastinum Target

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