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Cost-effective hybrid long-short read assembly delineates alternative GC-rich Streptomyces hosts for natural product discovery

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Synthetic and Systems Biotechnology 2023年第2期8卷 253-261页

作者： Elena Heng Lee Ling Tan Dillon W.P.Tay Yee Hwee Lim Lay-Kien Yang Deborah C.S.Seow Chung Yan Leong Veronica Ng Siew Bee Ng Yoganathan Kanagasundaram fong tian wong Lokanand Koduru Molecular Engineering Laboratory Institute of Molecular and Cell Biology(IMCB)Agency for ScienceTechnology and Research(A*STAR)61 Biopolis Drive#07-06ProteosSingapore138673Singapore Green Chemistry and Biocatalysis Institute of Sustainability for ChemicalsEnergy and Environment(ISCE2)Agency for ScienceTechnology and Research(A*STAR)8 Biomedical GroveNeuros#07-01Singapore138665Singapore Singapore Institute of Food and Biotechnology Innovation(SIFBI) Agency for ScienceTechnology and Research(A*STAR)31 Biopolis WayLevel 2NanosSingapore138669Singapore

With the advent of rapid automated in silico identification of biosynthetic gene clusters(BGCs),genomics pre-sents vast opportunities to accelerate natural product(NP)***,prolific NP producers,Strepto-myces,are exceptionally GC-rich(>80%)and highly repetitive within *** pose challenges in sequencing and high-quality genome assembly which are currently circumvented via intensive ***,we outline a more cost-effective workflow using multiplex Illumina and Oxford Nanopore sequencing with hybrid long-short read assembly algorithms to generate high quality *** protocol involves subjecting long read-derived assemblies to up to 4 rounds of polishing with short reads to yield accurate BGC *** successfully sequenced and assembled 8 GC-rich Streptomyces genomes whose lengths range from 7.1 to 12.1 Mb with a median N50 of 8.2 *** analysis revealed previous misrepresentation among these strains and allowed us to propose a potentially new species,Streptomyces *** comprehensive characterization of their biosynthetic,pan-genomic and antibiotic resistance features especially for molecules derived from type I polyketide synthase(PKS)BGCs reflected their potential as alternative NP ***,the genome assemblies and insights presented here are envisioned to serve as gateway for the scientific community to expand their avenues in NP discovery.

关键词： Streptomyces GC-Rich Hybrid assembly Biosynthetic gene cluster(BGC) Natural product(NP)chassis Type I polyketide Synthase(PKS)

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Tribological evaluation of thermoplastic polyurethane-based bearing materials under water lubrication:Effect of load,sliding speed,and temperature

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Friction 2024年第8期12卷 1801-1815页

作者： Shaoli JIANG Janet SSwong Debashis PUHAN tian YUAN Xiuqin BAI Chengqing YUAN State Key Laboratory of Maritime Technology and Safety Wuhan University of TechnologyWuhan 430063China Department of Mechanical Engineering Imperial College LondonLondon SW72AZUK Yusuf Hamied Department of Chemistry University of CambridgeLensfield RoadCambridge CB21EWUK

Polymers are widely used in bearing *** the case of water-lubricated stern tube bearings,thermoplastic polyurethane(TPU)-based composites are used due to their excellent wear resistance,corrosion resistance,and tunable mechanical *** tribological performance,however,depends on operating *** this work,TPU was blended with carbon fiber,graphene platelet,and ultra-high molecular weight polyethylene(UHMWPE).Friction tests of TPU based-composites against copper countersurface were carried out in water to mimic the actual operating conditions of the *** of the resulting contacts were in the boundary lubrication regime,in which friction was attributed to both contact mechanics of asperities as well as water *** results show that the viscoelasticity of TPU has a considerable impact on its tribological *** lubrication at 50°C promotes the softening of polymer surface material during sliding,resulting in higher fluctuation in the coefficient of friction and wear *** is attributed to the reduced thermomechanical *** addition,Schallamach waviness is observed on worn *** tribological properties of TPU are significantly improved by the inclusion of carbon fiber,graphene platelet,and *** formation of graphene transfer-layers and UHMWPE transfer film reduces friction and wear loss,while the inclusion of carbon fiber enhances wear resistance due to improved mechanical properties and load bearing capacity.

关键词： friction thermoplastic polyurethane(TPU) water-lubricated bearings graphene platelet carbon fibers ultra-high molecular weight polyethylene(UHMWPE)

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Application of artifcial intelligence in cataract management:current and future directions

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Eye and Vision 2024年第1期10卷 21-30页

作者： Laura Gutierrez Jane Sujuan Lim Li Lian Foo Wei Yan Ng Michelle Yip Gilbert Yong San Lim Melissa Hsing Yi wong Allan fong Mohamad Rosman Jodhbir Singth Mehta Haotian Lin Darren Shu Jeng Ting Daniel Shu Wei Ting Singapore Eye Research Institute SingaporeSingapore Singapore National Eye Center 11 Third Hospital AvenueSingapore 168751Singapore Academic Ophthalmology School of MedicineUniversity of NottinghamNottinghamUK Zhongshan Ophthalmic Center Sun Yet Sen UniversityGuangzhouChina

The rise of artifcial intelligence(AI)has brought breakthroughs in many areas of *** ophthalmology,AI has delivered robust results in the screening and detection of diabetic retinopathy,age-related macular degeneration,glaucoma,and retinopathy of *** management is another feld that can beneft from greater AI *** is the leading cause of reversible visual impairment with a rising global clinical *** diagnosis,monitoring,and surgical management are necessary to address this *** addition,patients in large developing countries often sufer from limited access to tertiary care,a problem further exacerbated by the ongoing COVID-19 *** on the other hand,can help transform cataract management by improving automation,efcacy and overcoming geographical ***,AI can be applied as a telediagnostic platform to screen and diagnose patients with cataract using slit-lamp and fundus *** utilizes a deep-learning,convolutional neural network(CNN)to detect and classify referable cataracts ***,some of the latest intraocular lens formulas have used AI to enhance prediction accuracy,achieving superior postoperative refractive results compared to traditional ***,AI can be used to augment cataract surgical skill training by identifying diferent phases of cataract surgery on video and to optimize operating theater workfows by accurately predicting the duration of surgical ***,some AI CNN models are able to efectively predict the progression of posterior capsule opacifcation and eventual need for YAG laser *** advances in AI could transform cataract management and enable delivery of efcient ophthalmic *** key challenges include ethical management of data,ensuring data security and privacy,demonstrating clinically acceptable performance,improving the generalizability of AI models across heterogeneous populations,and improving the trust of e

关键词： Artifcial intelligence Telemedicine Cataract Cataract screening Cataract surgery IOL calculations Biometry Machine learning

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Unsynchronized butyrophilin molecules dictate cancer cell evasion of Vγ9Vδ2 T-cell killing

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Cellular & Molecular Immunology 2024年第4期21卷 362-373页

作者： Zeguang Wu Qiezhong Lamao Meichao Gu Xuanxuan Jin Ying Liu Feng tian Ying Yu Pengfei Yuan Shuaixin Gao Thomas S.Fulford Adam P.Uldrich Catherine C.L wong Wensheng Wei Biomedical Pioneering Innovation Center Peking-Tsinghua Center for Life SciencesPeking University Genome Editing Research CenterState Key Laboratory of Protein and Plant Gene ResearchSchool of Life SciencesPeking University100871BeijingChina EdiGene Inc. Life Science ParkChangping District102206BeijingChina Center for Precision Medicine Multi-Omics Research Peking University Health Science CenterPeking University100191BeijingChina Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity The University of MelbourneParkvilleVIC3010Australia Cancer Immunology Program Peter MacCallum Cancer CentreMelbourneVICAustralia State Key Laboratory for Complex Severe and Rare DiseasesClinical Research InstitutePeking Union Medical College HospitalChinese Academy of Medical Science&Peking Union Medical CollegePeking-Tsinghua Center for Life Sciences100871BeijingChina

Vγ9Vδ2 T cells are specialized effector cells that have gained prominence as immunotherapy agents due to their ability to target and kill cells with altered pyrophosphate *** our effort to understand how cancer cells evade the cell-killing activity of Vγ9Vδ2 T cells,we performed a comprehensive genome-scale CRISPR screening of cancer *** found that four molecules belonging to the butyrophilin(BTN)family,specifically BTN2A1,BTN3A1,BTN3A2,and BTN3A3,are critically important and play unique,nonoverlapping roles in facilitating the destruction of cancer cells by primary Vγ9Vδ2 T *** coordinated function of these BTN molecules was driven by synchronized gene expression,which was regulated by IFN-γsignaling and the RFX ***,an enzyme called QPCTL was shown to play a key role in modifying the N-terminal glutamine of these BTN proteins and was found to be a crucial factor in Vγ9Vδ2 T cell killing of cancer *** our research,we offer a detailed overview of the functional genomic mechanisms that underlie how cancer cells escape Vγ9Vδ2 T ***,our findings shed light on the importance of the harmonized expression and function of gene family members in modulating T-cell activity.

关键词： Butyrophilin Vγ9Vδ2 T-cell Cancer-specific immune evasion Glutaminyl-peptide cyclotransferase-like Pyrophosphate metabolite Immunotherapy

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Flame Synthesis of Zr/ZSM-5 Catalysts with Tunable Acidity for the Oxidative Dehydrogenation of Propane to Propene

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Journal of Thermal Science 2024年第1期33卷 268-283页

作者： DANIEL Samuel FONZEU MONGUEN Cedric Karel WU Lingnan tian Zhenyu Institute of Engineering Thermophysics Chinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China

Tuning the surface acidity of ZSM-5 catalyst is essential to achieve desired propene selectivity and *** several ratios of Zr were utilized to modify ZSM-5 via flame spray pyrolysis technique coupled with a pulse spray evaporation *** interaction between Zr and ZSM-5 in the flame influenced the physicochemical and acidity properties of the Zr/*** increasing Zr ratio in ZSM-5 shows coated layers of irregular nano-sized Zr with an increase in crystallite sizes due to the synergetic effect between Zr and *** surface chemical analysis revealed increased lattice oxygen on the Zr modified ZSM-5(1:4) sample compared to other *** acidity analysis revealed the Lewis and Br?nsted acid distribution in the weak and medium acid sites on the catalyst ***,the increase in Zr loading decreased the concentration of Br?nsted acid sites and tuned the catalyst surface to more Lewis acidity,promoting propene selectivity and hindering the over-oxidation of *** modified ZSM-5 catalysts were examined in a fixed bed reactor within 300℃-700℃ at a gas hourly space velocity(GHSV) of 6000 mL·g(catalysts)^(-1)·h^(-1) for the oxidative dehydrogenation of propane(ODHP) to *** the catalysts,Zr/ZSM-5(1:4) exhibited the best propene yield, with 57.19% propane conversion and 75.54% selectivity to propene and the highest *** work provides a promising strategy for tuning the surface acidity of ZSM-5 with Zr for ODHP applications.

关键词： flame synthesis Zr modified ZSM-5 catalyst ODHP surface acidity lattice oxygen

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Nat Chem Biol:利用CRISPR-Cas9激活细菌中沉默的基因簇,有望发现新的药物

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现代生物医学进展 2017年第17期17卷 I0004-I0004页

作者： Mingzi M Zhang, Wan Lin Yeo, Ee Lui Ang, Huimin Zhao fong tian wong, Elena Heng Yajie Wang, Shangwen Luo, Ryan E Cobb, Behnam Enghiad, Huimin Zhao Yee Hwee Lim Metabolic Engineering Research Laboratory Science and Engineering Institutes Agency for Science Technology and Research (A*STAR) Singapore Molecular Engineering Lab Biomedical Science Institutes A*STAR Singapore Department of Chemical and Biomolecular Engineering University of Illinois at Urbana-Champaign Urbana Illinois USA Organic Chemistry Institute of Chemical and Engineering Sciences A*STAR Singapore.

为了抵抗疾病，很多医药库中的武器是从细菌当中获得的。如今，利用CRISPR-Cas9基因编辑技术。研究人员揭示出沉默基因中隐藏着的更多潜在的宝藏。作为一类常见的细菌。链霉菌被用来产生很多作为抗生素、抗癌试剂和其他药物的化合物。... 详细信息

为了抵抗疾病，很多医药库中的武器是从细菌当中获得的。如今，利用CRISPR-Cas9基因编辑技术。研究人员揭示出沉默基因中隐藏着的更多潜在的宝藏。作为一类常见的细菌。链霉菌被用来产生很多作为抗生素、抗癌试剂和其他药物的化合物。在一项新的研究中。来自美国伊利诺伊大学和新加坡科技研究局的研究人员利用CRISPR-Cas9技术激活链霉菌中不表达的或者说沉默的基因簇。

关键词：基因簇 Chem 细菌利用激活药物 Nat 编辑技术

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Effectiveness of post-COVID-19 primary care attendance in improving survival in very old patients with multimorbidity:a territory-wide target trial emulation

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Family Medicine and Community Health 2024年第3期12卷 25-35页

作者： Cuiling Wei Vincent Ka Chun Yan Camille Maringe Wenxin tian Rachel Yui Ki Chu Wenlong Liu Boyan Liu Yuqi Hu Lingyue Zhou Celine Sze Ling Chui Xue Li Eric Yuk Fai Wan Ching Lung Cheung Esther Wai Yin Chan William Chi Wai wong Ian Chi Kei wong Francisco Tsz Tsun Lai Centre for Safe Medication Practice and Research Department of Pharmacology and PharmacyLi Ka Shing Faculty of MedicineThe University of Hong KongHong KongHong Kong Department of Health Services Research and Policy Faculty of Public Health and PolicyLondon School of Hygiene and Tropical MedicineLondonUK School of Nursing Li Ka Shing Faculty of MedicineThe University of Hong KongHong KongHong Kong School of Public Health Li Ka Shing Faculty of MedicineThe University of Hong KongHong KongHong Kong Laboratory of Data Discovery for Health(D24H) Hong KongHong Kong Advanced Data Analytics for Medical Science(ADAMS)Limited Hong KongHong Kong Department of Medicine School of Clinical MedicineLi Ka Shing Faculty of MedicineThe University of Hong KongHong KongHong Kong Department of Family Medicine and Primary Care School of Clinical MedicineLi Ka Shing Faculty of MedicineThe University of Hong KongHong KongHong Kong Department of Pharmacy The University of Hong Kong-Shenzhen HospitalShenzhenPeople's Republic of China Aston Pharmacy School Aston UniversityBirminghamUK School of Pharmacy Macao University of Science and TechnologyTaipaMacao

Objectives Older individuals with multimorbidity are at an elevated risk of infection and complications from *** of post-COVID-19 interventions or care models in reducing subsequent adverse outcomes in these individuals have rarely been *** study aims to examine the effectiveness of attending general outpatient within 30 days after discharge from COVID-19 on 1-year survival among older adults aged 85 years or above with *** Retrospective cohort study emulating a randomised target trial using electronic health *** We used data from the Hospital Authority and the Department of Health in Hong Kong,which provided comprehensive electronic health records,COVID-19 confirmed case data,population-based vaccination records and other individual characteristics for the *** Adults aged 85 years or above with multimorbidity who were discharged after hospitalisation for COVID-19 between January 2020 and August *** Attending a general outpatient within 30 days of last COVID-19 discharge defined the exposure,compared to no outpatient *** outcome measures Primary outcome was all-cause mortality within one *** outcomes included mortality from respiratory,cardiovascular and cancer *** A total of 6183 eligible COVID-19 survivors were included in the *** all-cause mortality rate following COVID-19 hospitalisation was lower in the general outpatient visit group(17.1 deaths per 100 person-year)compared with non-visit group(42.8 deaths per 100 person-year).After adjustment,primary care consultations within 30 days after discharge were associated with a significantly greater 1-year survival(difference in 1-year survival:11.2%,95%CI 8.1%to 14.4%).We also observed significantly better survival from respiratory diseases in the general outpatient visit group(difference in 1-year survival:6.3%,95%CI 3.5%to 8.9%).In a sensitivity analysis for different grace period lengths

关键词： morbidity visit records

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High-Efficiency Dynamic Scanning Strategy for Powder Bed Fusion by Controlling Temperature Field of the Heat-Affected Zone

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Chinese Journal of Mechanical Engineering 2024年第1期37卷 203-214页

作者： Xiaokang Huang Xiaoyong tian Qi Zhong Shunwen He Cunbao Huo Yi Cao Zhiqiang Tong Dichen Li State Key Laboratory of Manufacturing Systems Engineering Xi’an Jiaotong UniversityXian 710049China

Improvement of fabrication efficiency and part performance was the main challenge for the large-scale powder bed fusion(PBF)*** this study,a dynamic monitoring and feedback system of powder bed temperature field using an infrared thermal imager has been established and integrated into a four-laser PBF equipment with a working area of 2000 mm×2000 *** heat-affected zone(HAZ)temperature field has been controlled by adjusting the scanning speed ***,the relationship among spot size,HAZ temperature,and part performance has been *** fluctuation of the HAZ temperature in four-laser scanning areas was decreased from 30.85℃to 17.41℃.Thus,the consistency of the sintering performance of the produced large component has been *** on the controllable temperature field,a dynamically adjusting strategy for laser spot size was proposed,by which the fabrication efficiency was improved up to 65.38%.The current research results were of great significance to the further industrial applications of large-scale PBF equipment.

关键词： Powder bed fusion Efficiency Large-scale Spot size Heat-affected zone(HAZ)

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食药同源健脾开胃物质及其成方制剂研究与应用进展

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中草药 2024年第6期55卷 2101-2112页

作者：田琦汤旗陈钧 wong LISOO 刘立丽吕正兵鲍康德浙江理工大学生命科学与医药学院浙江杭州310018 浙江省家蚕生物反应器和生物医药重点实验室浙江杭州310063 浙江国邦药业有限公司药物研究所浙江绍兴312369

传统中医认为“百病皆由脾胃衰而生”,现代社会因饮食不节、嗜烟好酒、作息无序及工作和生活压力等诸多因素,造成“脾失健运”。中医药学研究结果显示,健脾开胃的食品疗法因其耐受性及依从性好,被中医医家及患者广泛接受。通过对健脾开... 详细信息

传统中医认为“百病皆由脾胃衰而生”,现代社会因饮食不节、嗜烟好酒、作息无序及工作和生活压力等诸多因素,造成“脾失健运”。中医药学研究结果显示,健脾开胃的食品疗法因其耐受性及依从性好,被中医医家及患者广泛接受。通过对健脾开胃中药成方制剂、食药同源中药材及饮片和国内外食药同源政策进行综述,以促进食药物质在普通食品领域的开发,为健脾开胃类保健食品、特医食品等的研发提供理论依据。

关键词：食药同源健脾开胃食品疗法保健临床应用

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Unveiling the chemistry behind the electrolytic production of hydrogen peroxide by oxygenated carbon

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Journal of Energy Chemistry 2024年第9期96卷 49-58页

作者： Pan Xiang Kunshang Yang Qihao Yang Yang Gao Wenwen Xu Zhiyi Lu Liang Chen Zhiming Wang Ziqi tian Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of ChinaChengdu 610054SichuanChina Key Laboratory of Advanced Fuel Cells and Electrolyzers Technology of Zhejiang Province Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingbo 315201ZhejiangChina Institute for Advanced Study Chengdu UniversityChengdu 610106SichuanChina

Oxygenated carbon materials exhibit outstanding electrocatalytic performance in the production of hydrogen peroxide(H2O2)through a two-electron oxygen reduction *** nature of the active functional group and underlying reaction mechanism,however,remain ***,a comprehensive workflow was established to identify the active sites from the numerous possible *** common hydroxyl group at the notched edge demonstrates a key role in the two-electron *** local chemical environment weakens the binding of OOH intermediate to substrate while enhancing interaction with solution,thereby promoting the H_(2)O_(2)*** increasing pH,the intramolecular hydrogen bond between OOH intermediate and hydroxyl decreases,facilitating OOH ***,the rise in selectivity with increasing potential stems from the suppression of the four-electron *** active site was further validated through *** by theoretical understanding,optimal performance was achieved with high selectivity(>95%)and current density(2.06 mA/cm^(2))in experiment.

关键词： First-principles calculation Oxygen reductionreaction Hydrogen peroxide production Fixed-potential method Oxygenated carbon materials

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