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Physical Characteristics and the Effect of Boiling and Fermentation on the Nutritional Value of <i>Telfairia occidentalis</i>Seeds

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Journal of Agricultural Chemistry and Environment 2021年第4期10卷 389-401页

作者： Mercy Bih Loh Achu Ruth Viviane Djuikwo Stéphane Ghomsi Tamo Christiane Laure Maptouom Fotso Michelle Carole Djouhou Fowe elie fokou Laboratory for Food Science and Metabolism Department of Biochemistry Faculty of Science University of Yaoundé I Yaoundé Cameroon

This work evaluates some physical parameters (the weight, length, diameter of fruits and seeds, number of seeds per fruit) and the effect of boiling and fermentation on the nutritional value of Telfairia occidentalis (fluted pumpkin) seeds. Firstly, a survey was done in the city of Yaounde on the different treatments applied to the seeds before cooking. From the results of the survey, the seeds were divided into three groups: raw, boiled and fermented. The moisture, lipid, protein, fibre, carbohydrate and ash contents were analysed using AOAC methods and minerals by atomic absorption spectrophotometry. The results showed that T. occidentalis fruit averagely weighed 6.35 kg and contained about 90 seeds. The decorticated seeds had an ovoid shape, 3.70 cm long and weighed 8.91 g. Boiling led to an increase in lipid (16.29% - 31.44%) and carbohydrate (19.20% - 21.8%) but a decrease in protein (54.06% - 34.17%) contents. Fermentation increased the crude fibre (0.70% - 1.1%) but decreased the ash content (4.07% - 3.14% DM). Boiled seeds had higher calcium, magnesium, potassium and sodium, while fermented seeds had higher zinc levels. Boiling proved better in preserving most of the seed nutrients. These seeds could be used to prevent some mineral deficiencies and their high proteins suggest their potential for the formulation of infant foods.

关键词： Physical Parameters Nutritional Composition Boiling Fermentation Telfairia occidentalis Seeds

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Adverse Events Clustering with NAT2 Slow Metabolisers following Deparasitization in Children in Bangolan, NWR Cameroon

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Journal of Life Sciences 2013年第7期7卷 742-748页

作者： Olivia Afa Achonduh Babara Atogho-Tiedeu Innocent Ali Mbulli Jean Paul Chedjou Mercy Achu Akindeh Mbu Nji fokou elie Eric Kamgue Vera Veyee Orise Karana Delphine Sahfe Wilfred Fon Mbacham The Biotechnology Center University of Yaounde I Yaounde Cameroon Department of Biochemistry University of Yaounde L gaounde Cameroon Bangolan Baptist Integrated Health Center Health Board Cameroon Baptist Convention Bamenda Cameroon

Inter individual differences in the metabolism of antimalarials could be due to polymorphism of NAT2 gene. The authors determined the genotypic frequencies of single nucleotide polymorphism (SNP) of NAT2 gene and it"s implication in antimalarial treatment during a vitamin A and zinc supplementation intervention in children aged 6 to 24 months. Children were deparasitized with artesunate-amodiaquine (ASAQ)-toddler 50/135 mg. Pharmacovigilance was done for 40 days, adverse events recorded and blood was spotted on filter paper for DNA extraction by chelex method. PCR-RFLP was performed with restriction enzymes KpnI, TaqI, and BamHI for detection of SNPs of NAT2. Allelic frequencies and phenotypes were compared between participants with or without adverse drug events. The prevalence of fast, slow and intermediate acetylators was 55%, 30% and 11% respectively. There was a significant association (P = 0.035) between NAT2 slow acetylators (and susceptibility to develop skin rash. No significant difference was observed between fast and slow acetylators and susceptibility to develop fever, anorexia, cough and common cold. Slow acetylators were more susceptible, (P = 0.011) to develop any adverse event The NAT2 slow acetylator phenotype was the most predominant and individuals with this phenotype were more significantly susceptible to develop adverse events to ASAQ.

关键词：事件记录儿童代谢喀麦隆 PCR-RFLP 单核苷酸多态性基因多态性聚类

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Global prevalence of occult hepatitis C virus: A systematic review and meta-analysis

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World Journal of Methodology 2022年第3期12卷 179-190页

作者： Donatien Serge Mbaga Sebastien Kenmoe Jacky Njiki Bikoï Guy Roussel Takuissu Marie Amougou-Atsama Etienne Atenguena Okobalemba Jean Thierry Ebogo-Belobo Arnol Bowo-Ngandji Martin Gael Oyono Jeannette Nina Magoudjou-Pekam Ginette Irma Kame-Ngasse Alex Durand Nka Alfloditte Flore Feudjio Cromwel Zemnou-Tepap elie Adamou Velhima Juliette Laure Ndzie Ondigui Rachel Audrey Nayang-Mundo Sabine Aimee Touangnou-Chamda Yrene Kamtchueng Takeu Jean Bosco Taya-fokou Chris Andre Mbongue Mikangue Raoul Kenfack-Momo Cyprien Kengne-Ndé Carole Stephanie Sake Seraphine Nkie Esemu Richard Njouom Lucy Ndip Sara Honorine Riwom Essama Department of Microbiology The University of Yaounde IYaounde 00237Cameroon Department of Microbiology and Parasitology University of BueaBuea 00237Cameroon Department of Virology Centre Pasteur of CameroonYaounde 00237Cameroon Centre of Research in Food Food Security and NutritionInstitute of Medical Research and Medicinal Plants StudiesYaounde 00237Cameroon Centre de Recherche sur les Maladies Emergentes et Re-Emergentes Institute of Medical Research and Medicinal Plants StudiesYaounde 00237Cameroon Faculty of Medicine and Biomedical Science The University of Yaounde IYaounde 00237Cameroon Medical Research Centre Institute of Medical Research and Medicinal Plants StudiesYaounde 00237Cameroon Laboratory of Parasitology and Ecology The University of Yaounde IYaounde 00237Cameroon Department of Biochemistry The University of Yaounde IYaounde 00237Cameroon Virology Laboratory Chantal Biya International Reference Center for Research on HIV/AIDS Prevention and ManagementYaounde 00237Cameroon

BACKGROUND Occult hepatitis C infection(OCI)is characterized by the presence of hepatitis C virus(HCV)RNA in the liver,peripheral blood mononuclear cells(PBMC)and/or ultracentrifuged serum in the absence of detectable HCV-RNA in *** has been described in several categories of populations including hemodialysis patients,patients with a sustained virological response,immunocompromised individuals,patients with abnormal hepatic function,and apparently healthy *** To highlight the global prevalence of *** We performed a systematic and comprehensive literature search in the following 4 electronic databases PubMed,EMBASE,Global Index Medicus,and Web of Science up to 6th May 2021 to retrieve relevant studies published in the *** studies were unrestricted population categories with known RNA status in serum,PBMC,liver tissue and/or ultracentrifuged *** were extracted independently by each author and the Hoy et al tool was used to assess the quality of the included *** used the random-effect meta-analysis model to estimate the proportions of OCI and their 95%confidence intervals(95%CI).The Cochran's Q-test and the I2 test statistics were used to assess heterogeneity between *** plot and Egger test were used to examine publication bias.R software version 4.1.0 was used for all *** The electronic search resulted in 3950 *** obtained 102 prevalence data from 85 included *** pooled prevalence of seronegative OCI was estimated to be 9.61%(95%CI:6.84-12.73)with substantial heterogeneity[I^(2)=94.7%(95%CI:93.8%-95.4%),P<0.0001].Seropositive OCI prevalence was estimated to be 13.39%(95%CI:7.85-19.99)with substantial heterogeneity[I^(2)=93.0%(90.8%-94.7%)].Higher seronegative OCI prevalence was found in Southern Europe and Northern Africa,and in patients with abnormal liver function,hematological disorders,and kidney *** seropositive OCI prevalence was found in Southern Europe,Northern

关键词： Occult hepatitis C virus infection Prevalence Worldwide Peripheral blood mononuclear cells Hepatitis C virus

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