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mesenchymal stem cells secretome:The cornerstone of cell-free regenerative medicine

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World Journal of Stem Cells 2020年第12期12卷 1529-1552页

作者： Alberto González-González Daniel García-Sánchez monica Dotta JoséC Rodríguez-Rey flor m.pérez-campo Department of molecular Biology_IDIVAL Faculty of MedicineUniversity of CantabriaSantander 39011CantabriaSpain

mesenchymal stem cells(mSCs)are the most frequently used stem cells in clinical trials due to their easy isolation from various adult tissues,their ability of homing to injury sites and their potential to differentiate into multiple cell ***,the realization that the beneficial effect of mSCs relies mainly on their paracrine action,rather than on their engraftment in the recipient tissue and subsequent differentiation,has opened the way to cell-free therapeutic strategies in regenerative *** the soluble factors and vesicles secreted by mSCs are commonly known as *** secretome has a key role in cell-to-cell communication and has been proven to be an active mediator of immunemodulation and regeneration both in vitro and in ***,the use of secretome has key advantages over cell-based therapies,such as a lower immunogenicity and easy production,handling and ***,mSCs can be modulated to alter their secretome composition to better suit specific therapeutic goals,thus,opening a large number of *** these advantages now place mSCs secretome at the center of an important number of investigations in different clinical contexts,enabling rapid scientific progress in this field.

关键词： mesenchymal stem cells Secretome Soluble factors Extra-cellular vesicles Exosomes Bone regeneration

Communication between bone marrow mesenchymal stem cells and multiple myeloma cells:Impact on disease progression

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World Journal of Stem Cells 2023年第5期15卷 421-437页

作者： Daniel García-Sánchez Alberto González-González Ana Alfonso-Fernández mónica Del Dujo-Gutiérrez flor m.pérez-campo Department of molecular Biology_IDIVAL Faculty of MedicineUniversity of CantabriaSantander 39011CantabriaSpain Servicio de Traumatología y Cirugía Ortopédica Hospital Universitario Marqués de ValdecillaInstituto de Investigación Sanitaria Valdecilla(IDIVAL)Facultad de MedicinaUniversidad de CantabriaSantander 39008CantabriaSpain

multiple myeloma(mm)is a hematological malignancy characterized by the accumulation of immunoglobulin-secreting clonal plasma cells at the bone marrow(Bm).The interaction between mm cells and the Bm microenvironment,and specifically Bm mesenchymal stem cells(Bm-mSCs),has a key role in the pathophysiology of this *** data support the idea that Bm-mSCs not only enhance the proliferation and survival of mm cells but are also involved in the resistance of mm cells to certain drugs,aiding the progression of this hematological *** relation of mm cells with the resident Bm-mSCs is a two-way *** modulate the behavior of Bm-mSCs altering their expression profile,proliferation rate,osteogenic potential,and expression of senescence *** turn,modified Bm-mSCs can produce a set of cytokines that would modulate the Bm microenvironment to favor disease *** interaction between mm cells and Bm-mSCs can be mediated by the secretion of a variety of soluble factors and extracellular vesicles carrying microRNAs,long non-coding RNAs or other ***,the communication between these two types of cells could also involve a direct physical interaction through adhesion molecules or tunneling ***,understanding the way this communication works and developing strategies to interfere in the process,would preclude the expansion of the mm cells and might offer alternative treatments for this incurable disease.

关键词： multiple myeloma mesenchymal stem cells Bone marrow microenvironment Soluble factors Extra-cellular vesicles Cells adhesion molecules Tunnellingnanotubes

Cytotoxic effect of Spirulina platensis extracts on human acute leukemia Kasumi-1 and chronic myelogenous leukemia K-562 cell lines

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Asian pacific Journal of Tropical Biomedicine 2017年第1期7卷 14-19页

作者： flor Yohana flores Hernandez Sanghamitra Khandual Inocencia Guadalupe Ramírez López Center for Investigation and Assistance in Technology Design for State of JaliscoC.P.45019ZapopanJaliscoMexico

Objective: To evaluate the cytotoxic effects of Spirulina platensis extracts on acute leukemia Kasumi-1 and chronic leukemia K-562 cancer cell ***: Various concentrations of Spirulina platensis extracts(0.25–50.00 mg/m L)obtained with different solvents were used to treat cell lines for 72 h. For cytotoxic effect studies, cell viability test with trypan blue solution, mTT assay and microscopic cytomorphological assessment were ***: Spirulina extract obtained with 70% ethanol showed significant cytotoxicity in K562 and Kasumi-1 cell lines. With trypan blue solution, IC_(50) values were found to be4.64 mg/m L for K-562 and 3.68 mg/m L for Kusumi-1 cell lines. Spirulina aqueous extract also showed cytotoxicity with trypan blue method, at a slightly higher dose; where IC_(50) values were 12.68 mg/m L for K-562 and 2.13 mg/m L for Kusumi-1 cell lines. The IC_(50) values were found 0.40 mg/m L for K-562 and 0.31 mg/m L for Kusumi-1 cell lines for the 70% ethanol extract according to the mTT assay. Spirulina extract obtained with water also showed cytotoxicity but the dose was a little higher where IC_(50) values were15.77 mg/m L for K-562 and 9.44 mg/m L for Kusumi-1 cell lines. The effect of cytotoxicity with ethanol extract is quite comparable with that observed for cyclophosphamide, which is a chemical used as anticancer ***: The cytotoxicity exhibited by Spirulina extract to cancer cell lines might be due to the presence of phytopigments(carotenoids, chlorophyll, phycocyanin) as well as polysaccharides that were reported previously as constituents of the extract. So crude extracts of Spirulina can be used as a source to develop anticancer drugs.

关键词： Leukemia Cytotoxicity Spirulina Carotenoids

Degradation of duloxetine: Identification of transformation products by UHpLC-ESI(+)-HRmS/mS,in silico toxicity and wastewater analysis

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Journal of Environmental Sciences 2019年第8期31卷 113-123页

作者： Rodrigo A.Osawa Ana p.Carvalho Olinda C.monteiro m.Conceicao Oliveira m.Helena florêncio Centro de Química e Bioquímica Faculdade de Ciências Universidade de Lisboa Laboratório de FTICR e Espectrometria de massa Estrutural Faculdade de Ciências Universidade de Lisboa Centro de Química Estrutural Instituto Superior Técnico Universidade de Lisboa

Duloxetine(DUL), an antidepressant drug, has been detected in surface water and wastewater effluents, however, there is little information on the formation of its transformation products(Tps). In this work, hydrolysis, photodegradation(UV irradiation) and chlorination experiments were performed on spiked distillated water, under controlled experimental conditions to simulate abiotic processes that can occur in the environment and wastewater treatment plants(WWTps). Eleven Tps, nine from reaction with UV light and two from chlorine contact, were formed and detected by ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry, and nine of them had their chemical structures elucidated upon analyses of their fragmentation patterns in mS/mS spectra. The formation and degradation of the Tps were observed. The parent compound was completely degraded after30 min in photodegradation and after 24 hr in chlorination. Almost all Tps were completely degraded in the experiments. The ecotoxicity and mutagenicity of the Tps were predicted based on several in silico models and it was found that a few of these products presented more ecotoxicity than DUL itself and six Tps showed positive mutagenicity. Finally, wastewater samples were analyzed and DUL and one Tp, possibly formed by chlorination process, were detected in the effluent, which showed that WWTp not only did not remove DUL, but also formed a Tp.

关键词： High resolution mass spectrometry Duloxetine Degradation processes Transformation products Wastewater analysis In silico toxicity

Thermal and photoperiodic requirements of the seedling stage of three tropical forest species

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Journal of Forestry Research 2023年第1期34卷 209-220页

作者： Fabrina Bolzan martins mábele de Cássia Ferreira Flávia Fernanda Azevedo Fagundes Gabriel Wilson Lorena florêncio Natural Resource Institute Federal University of ItajubáItajubáMinas Gerais 37500-903Brazil

Air temperature and photoperiod play an important role in the seedling development for tropical forest *** variables are sensitive to climate,and so evaluating thermal and photoperiodic effects on seedling development is fundamental,especially for climate change *** to quantify thermal time and the energy required for plants to reach a development stage include air temperature and cardinal *** photoperiod will also affect physiological reactions of a plant and thus its *** we evaluated the six thermal time methods widely used to compute thermal requirement,and identified the influence of the photoperiod from the 2015 and2016 growing seasons and 12 sowing dates in Itajubá,minas Gerais state,Brazil,on seedling development of three native tropical forest species psidium guajava L.(myrtaceae),Citharexylum myrianthum Cham.(Verbenaceae),and Bixa orellana L.(Bixaceae).The method used to quantify thermal time influenced the analytical results of seedling development;the one that considered three cardinal temperatures and compared them with the mean air temperature(method5)performed better in computing thermal *** influence of photoperiod on seedling development was inconclusive for the three species,but all three developed better in mild temperatures(between 13.3℃and 26.9℃)with a photoperiod shorter than 13 h.

关键词： phyllochron Thermal time Day length Seedling stage phenology

Underlying mechanism of the hydrothermal instability of Cu3（BTC）2 metal-organic framework

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Frontiers of Chemical Science and Engineering 2016年第1期10卷 103-107页

作者： Nadeen AI-Janabi Abdullatif Alfutimie flor R. Siperstein Xiaolei Fan School of Chemical Engineering and Analytical Science The University of Manchester Oxford Road Manchester M13 9PL UK

Water induced decomposition of Cu3（BTC）2 （BTC= benzene-l,3,5-tricarboxylate） metal-organic framework （mOF） was studied using dynamic water vapour adsorption. Small-angle X-ray scattering, Fourier transform infrared spectroscopy and differential scanning calorimetry analyses revealed that the underlying mechanism of Cu3（BTC）2 mOF decomposition under humid streams is the interpenetration of water molecules into Cu-BTC coordination to displace organic linkers （BTC） from Cu centres.

关键词： metal-organic framework (mOF) open metal sites (OmSs) dynamic water vapour adsorption hydrothermal stability

phenotypic Variation among and within Three peppers Species(Capsicum)from mexico

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phyton-International Journal of Experimental Botany 2021年第1期90卷 259-275页

作者： Carlos Eduardo Ornelas-Ramírez Sergio Hernández-Verdugo Jesús Enrique Retes-manjarrez Angel Valdez-Ortiz Antonio pacheco-Olvera Tomás Osuna-Enciso flor porras Facultad de Agronomía Universidad Autónoma de SinaloaCuliacánSinaloa80000México programa Regional de posgrado en Biotecnología Facultad de Ciencias Químico-BiológicasUniversidad Autónoma de SinaloaCuliacánSinaloa80030México Centro de Investigación en Alimentación y Desarrollo A.C.Unidad Culiacán CuliacánSinaloa80110México

The phenotypic variation and its distribution among species,morphotypes,and among and within populations was estimated in 71 populations pertaining to 15 morphotypes of three domesticated species of Capsicum from *** were made in the states of Sinaloa,Nayarit,Oaxaca,Tabasco,and Yucatán in two agroecosystems:Backyard-garden and *** phenotypic characteristics were analyzed through one-way variance analysis and multivariate analyses of principal components analysis(pCA)and hierarchical clustering using Ward’s *** study was performed in a uniform greenhouse experiment.A high variation was found among and within populations in all the measured *** the total variation,13.0%was distributed among species,27.9%among morphotypes,8.1%among populations,and 51.0%within *** plants grew in a uniform environment,these results indicate that the differences observed among and within species,morphotypes,and populations have a genetic *** and multivariate analyses clearly differentiated morphotypes,suggesting that the category of morphotypes must be used to nominate the infraspecific variation in the domesticated *** principal components analysis identified a total of 15 principal components that contributed to explain the total *** first two components explained 59.64%of the total variation and seven components explained more than 90%of the total *** the measured characteristics,number of seed per fruit,weight of the fruit,width of the fruit,length of the fruit,stem diameter,days to flowering,and height of the plant contributed to component 1 variation,whereas width of the leaf,length of the leaf,and number of locules,number of fruits and number of seeds per plant contributed to component *** hierarchical clustering separated the populations and the morphotypes in two large different *** group consisted of populations collected in monoculture conditions and the oth

关键词： phenotypic variation Capsicum annuum Capsicum chinense Capsicum frutescens morphotype

phenotypic and genomic relationships between vulva score categories and reproductive performance in first-parity sows

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Journal of Animal Science and Biotechnology 2021年第2期12卷 479-491页

作者： flor-Anita Corredor Leticia p.Sanglard Jason W.Ross Aileen F.Keating Richard J.Leach Nick V.L.Serão Department of Animal Science Iowa State UniversityAmesIA 50011USA Iowa pork Industry Center Iowa State UniversityAmesIA 50011USA

Background:One of the biggest challenges in the swine industry is to increase female reproductive ***,vulva score categories(VSC),assessed prior to puberty,has been proposed as an indicator trait of efficient reproductive performance in *** objective of this study was to validate the use of VSC as an indicator trait for reproductive performance,and to perform genetic and genomic analyses for ***:The phenotypic relationship of VSC,using a three-point scale:small(VSC-S),medium(VSC-m),and large(VSC-L),on reproductive performance was evaluated on three *** was measured at 15 weeks of age,for farms 1 and 2,and at 14 weeks of age for farm 3 on 3981 Yorkshire gilts,in which 1083 had genotypes(~50 K SNps).Genetic parameters for VSC with reproductive traits were estimated using ssGBLUp.A Genome-wide association study(GWAS)for VSC was performed using ***:For the phenotypic analysis of VSC across datasets,differences in performance were identified there was a significant effect(p≤0.05)for the interaction between Farm and VSC for total number dead(TND),and a trend(pmber born(TNB).There were significant(p≤0.05)pre-defined contrasts of VSC-S versus VSC-m+L on TNB,number born alive(NBA),TND,number of stillborn(NSB),and number of mummies(mUm).Heritability estimates for VSC as a categorical trait(VSCc)and a quantitative trait(VSCq)were 0.40±0.02 and 0.83±0.02,respectively,for across farm,0.13±0.07 and 0.20±0.10,respectively,for Farm1,0.07±0.07 and 0.09±0.09,respectively,for Farm2,and 0.20±0.03 and 0.34±0.05,respectively,for *** across farms,favorable genetic correlations estimates were found for TNB(0.28±0.19)and NBA(0.26±0.17).Within farms,moderate genetic correlations between VSC with reproductive traits were found for TNB(0.61±0.47)and mUm(0.69±0.47)for farm 1,for number of services until first farrow(NS;0.69±0.38)and unique service with successful first farrow(SFS;−0.71±0.38)for farm *** genomic regions

关键词： Genetic parameters GWAS Reproduction Swine Vulva

Tree community composition,structure and diversity along an elevational gradient in an Andean forest of Northern Ecuador

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Journal of mountain Science 2021年第9期18卷 2315-2327页

作者： JImÉNEZ-pAZ Rosa WORTHY Samantha J VALENCIA Renato pÉrezÁlvaro J REYNOLDS Alex BARONE John A BURGESS Kevin S Laboratorio de Ecología de plantas Escuela de Ciencias BiológicasFacultad de Ciencias Exactas y NaturalesPontificia Universidad Católica del EcuadorQuito 17-01-2184Ecuador Department of Biology College of Letters&SciencesColumbus State UniversityColumbusGeorgia 31907USA Herbario QCA Escuela de Ciencias BiológicasFacultad de Ciencias Exactas y NaturalesPontificia Universidad Católica del EcuadorQuito 17-01-2184Ecuador The Lovett School AtlantaGeorgia 30327USA

The Andean forests of northern Ecuador are known for their high levels of plant diversity relative to the area they ***,these forests grow on steep slopes that lead to dramatic habitat gradients across short *** extreme habitat gradients make the Andean forest ecosystem an excellent natural laboratory for understanding the effect of elevation on forest community diversity,structure and *** established 31 plots(50 m×5 m)which are divided between two elevational transects in the cloud forest of the Siempre Verde Reserve in the western foothills of the Andes mountains of northern *** trees and tree ferns with a diameter at breast height(dbh)≥5 cm were measured and *** examined changes in community composition,structure,and diversity along and between the elevational transects and three elevational zones:low(2437–2700 m),middle(2756–3052 m),and high(3163–3334 m).We found four main trends associated with the elevational gradients at this site:(1)community composition differed between the two transects and among the three elevational zones according to N-mDS,ANOSIm,and percentage of shared species,with some species having limited distributions,(2)metrics of community structure showed opposite relationships with elevation,depending on the transect,with the only significant relationship(negative)found between basal area and elevation in the open trail transect,(3)alpha diversity,in general,peaked at mid-elevations,and(4)beta diversity consistently increased with distance between plots along *** complexity of changes in community composition,structure,and alpha diversity along elevation may be related to the heterogeneity of the environment on a local scale,such as topography,soil composition,and even human impact,or to dispersal limitation and should be investigated *** changes in community composition and the relatively high beta diversity found at this site exemplify the biological complexity of mon

关键词： Elevational gradient Cloud forest Andes Structure Composition Diversity