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A step-by-step protocol for formaldehyde-assisted isolation of regulatory elements from Arabidopsis thaliana

Journal of Integrative Plant Biology 2014年第6期56卷 527-538页

作者： Mohammad Amin Omidbakhshfard fla.ia.vischi winck Samuel Arvidsson Diego M.Ria?o-Pachón Bernd Mueller-Roeber University of Potsdam Institute of Biochemistry and Biology Potsdam-Golm 14476 Germany Max-Planck Institute of Molecular Plant Physiology Potsdam-Golm 14476 Germany Brazilian Bioethanol Science and Technology Laboratory (CTBE) Brazilian Center of Research in Energy and Materials (CNPEM) Campinas Brazil

The control of gene expression by transcriptional regulators and other types of functional y relevant DNA transactions such as chromatin remodeling and replication underlie a vast spectrum of biological processes in al organisms. DNA transactions require the control ed interaction of proteins with DNA sequence motifs which are often located in nucleosome-depleted regions （NDRs） of the chromatin. Formaldehyde-assisted isolation of regulatory elements （FAIRE） has been established as an easy-to-implement method for the isolation of NDRs from a number of eukaryotic organisms, and it has been successful y employed for the discovery of new regulatory segments in genomic DNA from, for example, yeast, Drosophila, and humans. Until today, however, FAIRE has only rarely been employed in plant research and currently no detailed FAIRE protocol for plants has been published. Here, we provide a step-by-step FAIRE protocol for NDR discovery in Arabidopsis thaliana. We demonstrate that NDRs isolated from plant chromatin are readily amenable to quantitative polymerase chain reaction and next-generation sequencing. Only minor modification of the FAIRE protocol wil be needed to adapt it to other plants, thus facilitating the global inventory of regulatory regions across species.

关键词： Arabidopsis thaliana chromatin cis-regulatory elements epigenomics FAIRE-qPCR FAIRE-seq gene expression gene regulatory network transcription factor

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Non-invasive respiratory support for COVID-19-related acute respiratory failure

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Chinese Medical Journal 2022年第4期135卷 416-418页

作者： João Carlos winck Joaquim Moreira Department of Medicine Faculty of Medicine-Porto UniversityPorto 4200-319Portugal Unidade Local de Saúde do Alto Minho Viana do CasteloPortugal

The European Respiratory Society/American Thoracic Society clinical practice guidelines,which are only recommendatory,suggest utilization of non-invasive respiratory support therapies(NRST)as a preventive strategy for avoiding intubation in hypoxemic acute respiratory failure(ARF).[1]Theoretical concerns against the use of NRST are essentially related to safety reasons for the risk of the virus spreading among healthcare workers(HCW)and for the risk of delaying intubation in case of failure.

关键词： respiratory acute clinical

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Perspectives of the Brazilian Poultry Industry for the Chinese Consumer Market

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Journal of Food Science and Engineering 2012年第1期2卷 24-37页

作者： Cesar Augustus winck Miguelangelo Gianezini Clandio Favarini Ruviarot Joao Armando Dessimon Machado Homero Dewes University of West of Santa Catarina Joagaba 89600-000 Brazil Center for Studies and Researches in Agribusiness Federal University of Rio Grande do Sul Porto Alegre 91540-000 Brazil

The objective of this article was to analyze the poultry and derivatives commerce between Brazil and China, taking into account the current market and the perspectives for the next years. The method used was the bibliographic review and analysis of secondary data from different sources, associated with the Market Orientation Theory and the Value Chain Theory. Nowadays, China is Brazil＇s main commercial partner in the international market, but taking into account the chicken meat and its derivatives, having Brazil as the first worldwide exporter and China, the second largest consumer, an effective commercial relationship has not been established yet. To understand the poultry market exchanges between Brazil and China, a framework was built to demonstrate the characteristics and factors found to influence country negotiations, considering current reality and future perspectives. Considering the Theory of Market Orientation, it is concluded that Brazil has effective conditions to consolidate itself as a major supplier of chicken meat and derivatives to China, because it shows determinant characteristics and market factors that allows the association of the national poultry value chain to the demands of the Chinese consumers, but some adjustments are needed in the sphere of international politics.

关键词： Chicken meat market orientation Brazil China agribusiness.

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Application of Non-Selective Herbicides in the Pre-Harvest of Wheat Damages Seed Quality

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American Journal of Plant Sciences 2018年第1期9卷 107-123页

作者： Glauber Moncon Fipke Thomas Newton Martin Ubirajara Russi Nunes Evandro Ademir Deak Jessica Deolinda Leivas Stecca Jose Eduardo Minussi winck Luiz Fernando Teleken Grando Anderson da Costa Rossato Post-Graduation Program in Agronomy at Department of Plant Science Federal University of Santa MariaSanta MariaBrazil Department of Plant Science Federal University of Santa MariaSanta MariaBrazil Laboratory of Seed Research and Teaching Federal University of Santa MariaSanta MariaBrazil Field Technical Manager Sementes Lanes Ltda.Sao GabrielBrazil Federal University of Santa Maria Santa MariaBrazil

Pre-harvest desiccation in order to minimize the time of exposure to abiotic factors after physiological maturity is a practice used in the production of quality seeds. The aim of this work was to evaluate the physical, physiological and sanitary quality of wheat seeds from plants that received non-selective herbicide application at pre-harvest. For this, the treatments were applied on pre-harvest culture. A completely randomized design was applied. We use the herbicides (glufosinate-ammonium, glyphosate and paraquat), phenological stages of herbicide application (Z-83, Z-85, Z-87, Z-92) and additional treatment (without application). The routine tests that express the physical, physiological and sanitary integrity of seeds were evaluated. For herbicide treatments, there was an 8% and 26% reduction in germination under laboratory conditions for BRS Parrudo and Sinuelo, respectively. Soil emergence was 14% higher for the control treatment, not statistically different for the Z-87 and Z-92 stages and also for the herbicides glufosinate-ammonium and glyphosate for both cultivars. The application of non-selective herbicides on wheat pre-harvest impairs the physical and physiological quality and promotes faster deterioration. Vigor is reduced under stress conditions by cold test and accelerated aging. There is no influence on seed sanity.

关键词： Glufosinate-Ammonium Glyphosate Paraquat Triticum aestivum(L)

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