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Tubastraea coccinea: A Non-Indigenous Coral (Cnidaria, Scleractinia) Collected at Arvoredo Island, South of Brazil with Potential MRSA and VRE Antimicrobial Activity

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Open Journal of Marine Science 2016年第2期6卷 334-340页

作者： Éverson Miguel Bianco Simone Quintana de Oliveira Tatiana da Rosa Guimarães Luiza Gabriela de Souza Pessoa Maria Eduarda Alves Santos Felipe Dal-Pizzol Eloir Paulo Schenkel flávio henrique reginatto Departamento de Ciências do Mar Universidade Federal de Sao Paulo Campus Baixada Santista Santos Brazil Programa de Pós-Graduacao em Farmácia Universidade Federal de Santa Catarina Florianópolis Brazil Molecular Invertebrate and Systematics Ecology Laboratory Department of Biology Chemistry and Marine Sciences University of the Ryukyus Nishihara Japan Departamento de Clínica Médica Universidade Federal de Santa Catarina Campus Universitário Trindade Florianópolis Brazil

Bacterial infection is considered to be one of the most critical health issues of the world. It is essential to overcome this problem by the development of new drugs. Marine organisms as corals, sponges, seaweeds, and other are an incredible source of novel pharmacologically active compounds. Herein, the antimicrobial activity (extract/fractions) of the invasive stony coralTubastraea coccinea was evaluated by the disk diffusion method against 21 microbial strains (ATCC and clinical strains). Micro broth dilution was used to determinate the MIC of the fractions that showed antimicrobial activity by the disk diffusion method. Bioautography assay was also performed. Our results showed that the n-butanol (BF) and aqueous fractions (AF) showed activity against ATCC strains Staphylococcus aureus (MIC 31.25 and 250 μg/mL),Salmonella typhimurium (MIC 125 and 500 μg/mL), Escherichia coli (MIC 62.5 and 500 μg/mL) and Pseudomonas aeruginosa (MIC 62.5 and 500 μg/mL), respectively. Moreover, BF fraction was also effective against the clinical strains S. aureus(MIC 62.5 μg/mL), Klebsiella pneumoniae Carbapenemase—KPC (MIC 125 μg/mL), Methicillin-Resistant Staphylococcus aureus—MRSA (MIC 125 μg/mL) and Vancomycin-Resistant Enterococcus faecalis—VRE (MIC 62.5 μg/mL). The ratio MBC/MIC reinforces the bactericidal profile of BF fraction. The bioautography assay of BF fraction showed the presence of antimicrobial components at Rf0.55.

关键词： Marine Natural Products Tubastraea coccinea Scleractinia Antimicrobial Activity Methicillin- Resistant Staphylococcus aureus (MRSA) Vancomycin-Resistant Enterococcus faecalis (VRE)

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Co-inoculation of selected nodule endophytic rhizobacterial strains with Rhizobium tropici promotes plant growth and controls damping off in common bean

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Pedosphere 2020年第1期30卷 98-108页

作者： Linnajara DE VASCONCELOS MARTINS FERREIRA Fernanda DE CARVALHO Júlia FONSECA COLOMBO ANDRADE Damiany PáDUA OLIVEIRA flávio henrique VASCONCELOS DE MEDEIROS Fatima Maria DE SOUZA MOREIRA Sector of Biology Microbiology and Biological ProcessesDepartment of Soil ScienceUniversidade Federal de LavrasLavras C.P.3037-37200-000(Brazil) Instituto Federal do Pará/IFPA Campus MarabáRuralMarabáC.P.041-68508-979(Brazil) Universidade Federal de Lavras Department of PhytopathologyLavras C.P.3037-37200-000(Brazil)

Production of common bean(Phaseolus vulgaris)is limited by the occurrence of damping off(rhizoctoniosis),which is caused by the fungus Rhizoctonia ***,the co-inoculation of plant growth-promoting rhizobacteria(PGPR)involved in biological control along with diatomic nitrogen(N2)-fixing rhizobia can enhance N nutrition and increase *** this context,finding microorganisms with synergistic effects that perform these two roles is of fundamental importance to ensure adequate yield *** aim of this study was to evaluate the effects of co-inoculation of nodule endophytic strains of the genera Bacillus,Paenibacillus,Burkholderia,and Pseudomonas with Rhizobium tropici CIAT 899,an N2-fixing rhizobial strain,on the biocontrol of damping off and growth promotion in common bean *** experiments were conducted under axenic conditions using the common bean cultivar Pé*** first experiment evaluated the potential of the 14 rhizobacterial strains,which were inoculated alone or in combination with CIAT 899,for the control of *** second experiment evaluated the ability of these 14 rhizobacterial strains to promote plant growth with three manners of N supply:co-inoculation with CIAT 899 at low mineral N supply(5.25 mg N mL^-1),low mineral N supply(5.25 mg N mL^-1),and high mineral N supply(52.5 mg N mL^-1).The use of rhizobacteria combined with rhizobia contributed in a synergistic manner to the promotion of growth and the control of damping off in the common ***-inoculation of the strains UflA 02-281/03-18(Pseudomonas sp.),UflA 02-286(Bacillus sp.),and UflA 04-227(Burkholderia fungorum)together with CIAT 899 effectively controlled damping *** the common bean,mineral N supply can be replaced by the co-inoculation of CIAT 899 with plant growth-promoting strains UflA 02-281/02-286/02-290/*** endophytes UflA02-281/02-286 are promising for co-inoculation with CIAT 899 in the common bean,promoting synergy with rhizobial i

关键词： Bacillus sp. biological control Burkholderia fungorum CIAT 899 Phaseolus vulgaris plant growth-promoting rhizobacteria Pseudomonas sp. Rhizoctonia solani

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Free amino acid content in trunk,branches and branchlets of Araucaria angustifolia(Araucariaceae)

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Journal of Forestry Research 2018年第6期29卷 1489-1496页

作者： Crizane Hackbarth Patricia Soffiatti fla′vio Zanette Eny Iochevet Segal floh Amanda Ferreira Macedo henrique Aparecido Laureano Departamento de Fitotecnia e Fitossanitarismo Federal University of Parana(UFPR)Rua dos Funcionarios1540JuveveCuritibaPR 81531-990Brazil Departamento de Botanica Federal University of Parana(UFPR)Av.Cel.Francisco H.dos Santos100Jardim das AmericasCuritibaPR 81531-980Brazil Instituto de Biociencias University of Sao PauloRua do Matao277Sao PauloSP 05508-090Brazil Departamento de Estatstica Federal University of Parana(UFPR)Av.Cel.Francisco H.dos Santos100Jardim das AmericasCuritibaPR 81531-980Brazil

Araucaria angustifolia(Bertol.) O. Kuntze exhibits dimorphism in its stem structure, where the trunk is orthotropic and branches and branchlets(primary and secondary branches) are plagiotropic. These stems exhibit different behavior when used for vegetative propagation,and only segments of trunk can form a complete plant. The physiological and biochemical mechanisms that characterize these stems are still little known. The aim of this study was to describe the free amino acid profiles in trunks,branches, and branchlets of A. angustifolia. Segments of 5 cm in length were excised from young individuals below the stem apex. The needles were removed and samples were frozen and lyophilized. The determinations were made by high-performance liquid chromatography, and the results were expressed as lg/g fresh weight(FW). The trunks and branches had the highest content of total amino acids, which were 112.23 ± 20.57 lg/g FW and 111.97 ± 27.78 lg/g FW, respectively. The amino acids—glutamine, aspartate and c-aminobutyric acid and tyrosine—were noticeably higher in the three types of *** the trunk, a higher amount of asparagine and tryptophan,was also detected. Glutamic acid and glutamine were found in higher quantities in the branches. The branchlets had very low total amino acid content (30.79 ± 4.19 lg/g FW), wherein asparagine is the only amino acid not detected. Thus, it was observed that the profile of the free amino acid differs among trunks, branches, and branchlets in A. angustifolia, indicating that they perform different functions.

关键词： Brazilian pine Physiological mechanisms Stem’s dimorphism Free amino acids

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Circulating leptin and its muscle gene expression in Nellore cattle with divergent feed efficiency

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Journal of Animal Science and Biotechnology 2018年第1期9卷 228-232页

作者： Lúcio flávio Macedo Mota Cristina Moreira Bonafé Pamela Almeida Alexandre Miguel henrique Santana Francisco JoséNovais Erika Toriyama Aldrin Vieira Pires Saulo da Luz Silva Paulo Roberto Leme JoséBento Sterman Ferraz Heidge Fukumasu Departmento de Zootecnia Universidade Federal dos Vales do Jequitinhonha e MucuriDiamantinaMG 39100 000Brazil Faculdade de Ciencias Agrarias e Veterinarias Universidade Estadual Paulista JaboticabalSP 14884 900Brazil Departamento de Medicina Veterinaria Faculdade de Zootecnia e Encjenharia de AlimentosUniversidade de Sao PauloAM.Duque de Caxias Norte n225Pirassununcja 13635 900SPBrazil Departmento de Zootecnia e Desenvolvimento Acjrossocioambiental Sustentavel Faculdade de VeterinariaUniversidade Federal FluminenseNiteroiRJ 24230 340Brazil Departamento de Zootecnia Faculdade de Zootecnia e Engenharia de AlimentosUniversidade de Sao PauloPirassununga 13635-900SPBrazil

Background： Leptin has a strong relation to important traits in animal production, such as carcass composition,feed intake, and reproduction. It is mainly produced by adipose cells and acts predominantly in the *** this study, circulating leptin and its gene expression in muscle were evaluated in two groups of young Nellore bulls with divergent feed efficiency. Individual dry matter intake（DMI） and average daily gain（ADG） of 98 Nellore bulls were evaluated in feedlot for 70 d to determinate the residual feed intake（RFI） and select 20 animals for the high feed efficient（LRFI） and 20 for the low feed efficient（HRFI） groups. Blood samples were collected on d 56 and at slaughter（80 d） to determine circulating plasma leptin. Samples of Longissimus dorsi were taken at slaughter for leptin gene expression ***： DMI and RFI were different between groups and LRFI animals showed less back fat and rump fat thickness,as well as less pelvic and kidney fat weight. Circulating leptin increased over time in all animals. Plasma leptin was greater in LRFI on 56 d and at slaughter（P = 0.0049）. Gene expression of leptin were greater in LRFI animals（P = 0.0022） in accordance with the plasma levels. The animals of the LRFI group were leaner, ate less, and had more circulating leptin and its gene ***： These findings demonstrated that leptin plays its physiological role in young Nellore bulls, probably controlling food intake because feed efficient animals have more leptin and lower residual feed intake.

关键词： Beef cattle Energy homeostasis Fat depositon Residual feed intake

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Intravascular Access in Chelonia mydas and Dermochelys coriacea Using the Seldinger Technique U Itrasou nd-Guided

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Journal of Life Sciences 2014年第5期8卷 385-393页

作者： Gustavo henrique Pereira Dutra Fálbio Futema flávio Augusto Marques dos Santos Cristiane Lassalvia Aquário Municipal de Santos Av. Bartolomeu de Gusmao s/n. Ponta da Praia. Santos-SP 11030-500 Brazil Universidade de Guarulhos Av. Anton Philips 01 Vila Herminia Guarulhos 07030-010 Brazil Universidade Anhembi Morumbi Rua Casa do Ator 294. Vila Olimpia 04546-000-Sao Paulo SP Brasil

Strand-ed turtles need fluid parenterally. The jugular access is best for the maintenance and patency of the catheter. The Seldinger technique guided by ultrasound seems to be the safest for catheter insertion. Five juveniles Chelonia mydas with fibropapillomatosis and an adult male leatherback turtle （Dermochelys coriacea） with altered buoyancy were sent to Santos Municipal Aquarium for rehabilitation. Turtles underwent catheterization of the jugular vein using the Seldinger technique with central venous polyurethane catheter monolumen 14 G to Dermochelys and 18 G for Chelonia, guided by ultrasound with 10 MHz transducer catheters were sutured to the skin and animals were subjected to fluid. In all turtles, the catheters were patency and were well established. There were no granulomatous reactions or related infections. The Dermochelys improved clinically after fluid resuscitation, and the catheter was removed one week after. In one Chelonia excision was 21% of its weight in tumors, and the animals received colloid catheter by enabling the mitigation of bleeding due to removal. The use of ultrasonography has enabled monitoring of all access as the patency of the catheter and fluid diffusivity.

关键词： Sea turtles Chelonia Dermochelys ultrassonography Seldinger technique.

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2-D DOA Estimation in a Cuboid Array Based on Metaheuristic Algorithms and Maximum Likelihood

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International Journal of Communications, Network and System Sciences 2020年第8期13卷 121-137页

作者： Gilberto Lopes Filho Ana Cláudia Barbosa Rezende Lucas Fiorini Cruz flávio henrique Teles Vieira Rodrigo Pinto Lemos Universidade Federal de Goiás (UFG) Goiánia Brazil

This paper proposes to apply the genetic algorithm and the firefly algorithm to enhance the estimation of the direction of arrival (DOA) angle of electromagnetic signals of a smart antenna array. This estimation is essential for beamforming, where the antenna array radiating pattern is steered to provide faster and reliable data transmission with increased coverage. This work proposes using metaheuristics to improve a maximum likelihood DOA estimator for an antenna array arranged in a uniform cuboidal geometry. The DOA estimation performance of the proposed algorithm was compared to that of MUSIC on different two dimensions scenarios. The metaheuristic algorithms present better performance than the well-known MUSIC algorithm.

关键词： Metaheuristic Algorithms Genetic Algorithm Firefly Algorithm DOA Estimation Maximum Likelihood

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Light Front Fermion Model Propagation

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Communications in Theoretical Physics 2013年第7期60卷 55-68页

作者： Jorge henrique Sales Alfredo Takashi Suzukil Universidade Estadual de Santa Cruz km 16 Rodovia Jorge Amado-45662-900-Ilhéus BA Brazi Physics and Engineering Department Southern Adventist University 4881 Taylor Circle-Collegedale TN 37315 USA Instituto de Física Terica UNESP-Universidade Estadual Paulista Rua Dr. Bento Teobaldo Ferraz 271-Bloco II Barra Funda 01140-070 So Paulo So Paulo Brazil

In this work we consider the propagation of two fermion fields interacting with each other by the exchange of intermediate scalar bosons in the light front. We obtain the corrections up to fourth order in the coupling... 详细信息

关键词： light front Fermions propagations

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Light Front Boson Model Propagation

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Communications in Theoretical Physics 2011年第6期55卷 1029-1040页

作者： Jorge henrique Sales Alfredo Takashi Suzuki Universidade Estadual de Santa Cruz km 16 Rodovia Ilheus-Itabuna-45662-900-IlheusBABrazil Instituto de Fisica Teorica UNESP-Universidade Estadual PaulistaRua Dr.Bento Teobaldo Ferraz 271-Bl.ⅡBarra Funda01140-070Sao PauloSao PauloBrazil

The scope and aim of this work is to describe the two-body interaction mediated by a particle （either the scalar or the gauge boson） within the light-front formulation. To do this, first of all we point out the importance of propagators and Green functions in Quantum Mechanics. Then we project the covariant quantum propagator onto the light front time to get the propagator for scalar particles in these coordinates. This operator propagates the wave function from x＋ = 0 to x＋〉 0. It corresponds to the definition of the time ordering operation in the light front time x＋. We calculate the light-front Green＇s function for 2 interacting bosons propagating forward in x＋. We also show how to write down the light front Green＇s function from the Feynman propagator and finally make a generalization to N bosons.

关键词： insert light-front bosons propagation

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Invasive ant learning is not affected by seven potential neuroactive chemicals

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Current Zoology 2024年第1期70卷 87-97页

作者： henrique Galante Tomer J.Czaczkes Department of Zoology and Evolutionary Biology Animal Comparative Economics LaboratoryUniversity of Regensburg93053 Regensburg Germany

Argentine ants Linepithema humile are one of the most damaging invasive alien species *** or disrupting cognitive abilities,such as learning,has the potential to improve management efforts,for example by increasing preference for a bait,or improving ants'ability to learn its characteristics or ***-feeding insects are often the victims of psychoactive manipulation,with plants lacing their nectar with secondary metabolites such as alkaloids and non-protein amino acids which often alter learning,foraging,or ***,the effect of neuroactive chemicals has seldomly been explored in ***,we test the effects of seven potential neuroactive chemicals-two alkaloids:caffeine and nicotine;two biogenic amines:dopamine and octopamine,and three nonprotein amino acids:β-alanine,GABA and taurineon the cognitive abilities of invasive *** using bifurcation *** results confirm that these ants are strong associative learners,requiring as little as one experience to develop an ***,we show no short-term effect of any of the chemicals tested on spatial learning,and in addition no effect of caffeine on short-term olfactory *** lack of effect is surprising,given the extensive reports of the tested chemicals affecting learning and foraging in *** mismatch could be due to the heavy bias towards bees in the literature,a positive result publicationbias,ordifferences inmethodology.

关键词： associativelearning caffeine memory neuroactivechemicals

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Ultrastructural features of the hypopharyngeal glands in the social wasp Polistes versicolor （Hymenoptera： Vespidae）

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Insect Science 2008年第3期15卷 277-284页

作者： Fabio Barros Britto flavio henrique Caetano Universidade Estadual Paulista Julio de Mesquita Filho Instituto de Bioci~ncias de Rio Claro - Depto. de Biologia Rio Claro SP Brazil

The wasps of the genus Polistes have been considered the key to understanding the evolution of social behavior in Hymenoptera. Several studies have shown that the development of organized insect societies was accompanied by the evolution of structures like exocrine glands, which became specialized to perform specific functions. This article investigates the ultrastructural and cytochemical features of the hypopharyngeal glands of Polistes versicolor. These glands have been studied in depth in social bees, where they occur only in nurses and produce the royal jelly. Our results＇ revealed that these glands basically did not vary among individuals or between sexes. They are constituted by spherical cells, each with a large nucleus and well-developed rough endoplasmic reticulum. Secretion vesicles are abundant, but lipid droplets were not observed, indicating that these glands may not have a role in pheromone synthesis. Acid phosphatase was detected in lysosomes, and also flee in the cytosol, but did not seem to be related with cell death. Thus, our results suggest that the hypopharyngeal glands ofP. versicolor may not have a specialized social role, but could produce digestive enzymes.

关键词： cellular cycle cytochemistry hypopharyngeal glands morphology Polistes,wasps

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