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Implant surface features as key role on cell behavior

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BIOCELL 2022年第5期46卷 1151-1156页

作者： RAFAEL SCAF DE MOLON MARTA maria ALVES pereira ERICA DORIGATTI DE AVILA Federal University of Rio de Janeiro Rio de Janeiro21941-617Brazil São Paulo State University Araraquara14801-930Brazil

It has been recognized that physical and chemical properties of biomaterial surfaces mediate the quality of extracellular matrix(ECM)that may affect cell *** nature,ECM is a heterogeneous three-dimensional superstructure formed by three major components,glycosaminoglycan,glycoconjugate,and protein,that anchors cellular compartments in tissues and regulates the function and the behavior of *** in the biointerface alter the quality of ECM and morphology through cell surface receptors,which,in turn,enable it to trigger specific cell signaling and different cellular *** fact,a number of strategies have been used to improve the functionality of surfaces and direct cell behavior through precisely designed ***,we aimed to discuss,through a science-based viewpoint,the biomaterial surface features on cell behavior and analyze the impact of cell physical modification on dental implant development.

关键词： Biomaterial Cell adhesion Surface chemistry Surface topography

Chemical composition, antiparasitic and cytotoxic activities of aqueous extracts of Ziziphus joazeiro Mart

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Asian Pacific Journal of Tropical Biomedicine 2019年第5期9卷 222-226页

作者： Jacqueline Cosmo ANDrade Ana Raquel pereira da Silva Ant?nia Thassya Lucas dos Santos maria Audilene Freitas Yedda maria Lobo Soares de Matos maria flaviana Bezerra Morais Braga Camila Fonseca Bezerra maria Isabeli pereira Gon?alo maria Celeste Vega Gomez Míriam Rolóm Cathia Coronel Paulo Riceli Vasconcelos Ribeiro Edy Sousa de Brito Henrique Douglas Melo Coutinho Microbiology and Molecular Biology Laboratory Biological Chemistry Department Regional University of Cariri-URCA Bioassays Laboratory-LABIO Federal University of Cariri-UFCA Brejo Santo University campus (CE) Center for the Development of Scientific Research Moisés Bertoni Foundation/Díaz Gill Laboratories Natural Products Chemistry Multiuser Laboratory LMQPN Embrapa Tropical Agroindustry

Objective: To compare the in vitro antiparasitic activity of aqueous extracts from Ziziphus joazeiro leaves and stem bark against Trypanosoma cruzi, Leishmania braziliensis, and Leishmania infantum, as well as to evaluate its cytotoxicity in mammalian cells, in addition to identifying the chemical composition of the extracts. Methods: Ziziphus joazeiro leaf and stem bark aqueous extracts were prepared by cold extraction maceration and subjected to ultra-efficient liquid chromatography coupled to a quadrupole/time of flight system. The susceptibility assays used Trypanosoma cruzi CLB5 strains and promastigote forms of Leishmania braziliensis and Leishmania infantum for antiparasitic activity of the extracts. Moreover, mammalian fibroblasts NCTC clone 929 were used for cytotoxicity analysis. Results: Terpenoid compounds, flavonoids and phenolic acid were identified in extracts. The stem bark aqueous extracts presented more significant results in terms of antiparasitic activity compared with the leaf aqueous extracts, especially against Leishmania braziliensis and Leishmania infantum promastigote forms with an IC50 < 500 μg/mL. The cytotoxicity evaluation showed moderate toxicity of the stem bark aqueous extracts, which is relevant information for the rational use of this plant part since it is widely used by the population. Conclusions: These preliminary results may contribute to the formulation of new therapeutic agents against this group of neglected diseases, so further investigations are required to delineate the mechanisms of action mainly of the aqueous extract of stem bark of Ziziphus joazeiro.

关键词： Antiepimastigote Antipromastigote UPLC-MS-ESI-QTOF

Protective Properties in Hymenaea martiana Hayne against Multi-drug-resistant Staphylococcus aureus

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Veterinary Science Research 2020年第2期2卷 16-23页

作者： Dielson da Silva Vieira Tânia maria Sarmento da Silva Timothy AHackett marianade Barros Weslen Fabrício Pires Teixeira Juliana Campos pereira Diniz Pedro HenriqueGorni Sanely Lourenço da costa maria Aparecida Scatamburlo Moreira MateusMatiuzzi da costa Francisco Leydson Formiga Feitosa São Paulo State University“Júlio de Mesquita Filho”(UNESP) School of Veterinary MedicineAraçatubaSao PauloBrazil Federal Rural University of Pernambuco(UFRPE) Department of Molecular SciencesRecifePernambucoBrazil Department of Biochemistry University of Nebraska-LincolnLincolnNE68508USA Federal University of Viçosa(UFV) Veterinary DepartmentViçosaMinas GeraisBrazil Federal University of Vale do São Francisco(UNIVASF) Department of Animal SciencePetrolinaPernambucoBrazil University of West Paulista(UNOESTE) Department of AgronomyPresidente PrudenteSao PauloBrazil Federal University of Goiás(UFG) Department of Veterinary MedicineGoiâniaGoiásBrazil

Antibiotic resistance represents a widespread problem in milk *** identification of compounds for a topically applied ointment used in mastitis therapy remains *** from the genus Hymenaea can be administered in cases of multi-drugresistant Staphylococcus aureus infection for ruminant species,but the protective properties are not well *** this research the aim is verify the protective effects of *** against *** infection in bovine mammary epithelial cell line(MAC-T)and to obtain an antioxidant profile evaluation in *** MAC-T cells were challenged with *** after being exposed to the extract of the *** in the protective *** the verification of the viability of the MAC-T cells,the MTT assay was performed,and was used dilutions of the plant extract,starting at 2.5%.The extract of *** was evaluated for antioxidant aspect in different dilutions by FRAP,ORAC and DPPH.A variety of flavonoids(quercetin,luteolin,etc.)have been identified as the main components by using mass spectrometry,reinforcing our in vitro findings that flavonoids,especially quercetin,have a medicinal profile capable of killing mastitis-causing *** excellent antioxidant pattern was observed in the 2.5%solution;however,membrane integrity in MAC-T cells was *** findings suggest low dilutions of *** extract has a desirable protective effect from *** *** in vitro studies can be gleaned upon for further in vivo studies.

关键词： dairy animal Fitoterapic Hymenaea gender Cell activity Mastitis

Analysis of genetic relationship among Arbutus unedo L. genotypes using RAPD and SSR markers

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Journal of Forestry Research 2013年第2期24卷 227-236页

作者： Filomena Gomes Rita costa maria M. Ribeiro Elisa Figueiredo Jorge M. Canhoto CERNAS Departamento Florestal Escola Superior Agrária Coimbra INRB Instituto Nacional de Recursos Biológicos Departamento de Recursos Naturais e Desenvolvimento Sustentável Escola Superior Agrária Centre of Functional Ecology Department of Life Sciences University of Coimbra

The strawberry tree （Arbutus unedo L.） is an underutilized, drought tolerant, fire resistant species with a south western distribution in Europe, and with ecological and putative socio-economical impact in Portugal and Mediterranean countries. Our aim was to develop an appropriate set of molecular markers to enable genetic diversity to be assessed and to fingerprint Arbutus unedo genotypes for breeding and conservation purposes in Portugal. Twenty-seven trees from a broad geographic range were screened with 20 random amplified polymorphic DNA （RAPD primers） and 11 microsatellite markers （SSR）. The RAPDs generated 124 bands, 57.3% of which were polymorphic, with an expected heterozygosity of 27%. We cross-amplified 11 SSR primers developed for Vaccinium spp., and 5 were found to be polymorphic in A. unedo, with 75% of expected heterozygosity, a number of alleles of 11.6, a null allele frequency of 7.6% and a polymorphic information content of 71%. Although the SSRs were more polymorphic and informative than the RAPDs, both markers displayed high genetic variability with the gathered data. No geographic pattern was observed in the genetic variation distribution based on both marker systems, and the lack of correlation between genetic and geographical matrices was confirmed by Mantel tests. Likely, no correlation was found between pairwise SSR and RAPD band-sharing matrices. These results and their implications on A. unedo breeding and conservation programs are discussed.

关键词： Ericaceae fingerprinting geographic pattern molecular markers strawberry tree.

Are extrafloral nectaries efficient against herbivores? Herbivory and plant defenses in contrasting tropical species

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Journal of Plant Ecology 2020年第4期13卷 423-430页

作者： Cássio Cardoso pereira maria Gabriela Boaventura Gislene Carvalho de Castro Tatiana Cornelissen Departamento de Genética Ecologia e EvoluçãoUniversidade Federal de Minas Gerais31270-910 Belo HorizonteMGBrazil Departamento de Ciências Naturais Universidade Federal de São João del-Rei36301-160 São João del-ReiMGBrazil

Aims Plants have limited resources for defenses and species that invest in biotic defenses might exhibit leaves that invest less in other types of *** have investigated whether plants that have few mechanical defenses,but have extrafloral nectaries(EFNs)patrolled by ants,are less prone to herbivory,compared with plants without EFNs that have tougher *** data from the literature were extracted to examine the reported levels of herbivory in plants with or without *** a savanna vegetation in southern Brazil,field data were collected in leaves from six tropical species and herbivory and specific leaf area(SLA)levels were *** further evaluated differences in herbivory and SLA among species and between plants with or without *** order to test the relationship between herbivory and leaf toughness we regressed average herbivory and average SLA per *** Findings Plants exhibited variable levels of leaf damage,but plants without ant defenses experienced the highest levels of leaf area loss to *** of mechanical defenses were also variable among the plant *** without EFNs were tougher,exhibiting lower values of *** plants without EFNs had more sclerophyllous leaves,this mechanical defense was not sufficient to impair and/or reduce herbivore feeding,suggesting that the biotic defenses performed by patrolling ants might be more effective than investment in mechanical defenses associated with leaf palatability.

关键词： ants biotic defenses herbivory leaf palatability specific leaf area

Laparoscopic and endoscopic cooperative surgery for full-thickness resection and sentinel node dissection for early gastric cancer

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World Journal of Gastrointestinal Endoscopy 2022年第8期14卷 508-511页

作者： Serafino Vanella maria Godas Joaquim costa pereira Ana pereira Ivano Apicella Francesco Crafa Department of General and Oncology Surgery St.Giuseppe Moscati HospitalAvellino 83100Italy Department of General Surgery Hospital de BragaBraga 4710-243Portugal

The endoscopic submucosal dissection(ESD)technique has become the gold standard for submucosal tumors that have negligible risk of lymph node metastasis(LNM),due to its minimal invasiveness and ability to improve quality of ***,this technique is limited in stage T1 cancers that have a low risk of *** full thickness resection can be achieved with laparoscopic endoscopic cooperative surgery(LECS),which combines laparoscopic gastric wall resection and *** LECS,the surgical margins from the tumor are clearly achieved while performing organ-preserving *** overcome the limitation of classical LECS,namely the opening of the gastric wall during the procedure,which increases the risk of peritoneal tumor seeding,non-exposed endoscopic wall-inversion surgery was *** this full-thickness resection technique,contact between the intra-abdominal space and the intragastric space was eliminated.

关键词： Endoscopic submucosal dissection Laparoscopic endoscopic cooperative surgery Non-exposed endoscopic wall-inversion surgery Early gastric cancer Nodal basin evaluation

Simulated Experiments for Teaching Mutually-Coupled Circuits CAD Techniques Using Analytic and Finite Element Solutions

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Journal of Electromagnetic Analysis and Applications 2017年第11期9卷 183-202页

作者： Antonio flavio Licariao Nogueira Vitor Horbe pereira da costa Rodolfo Lauro Weinert Santa Catarina State University JoinvilleBrazil

The paper describes an approach to teaching mutually-coupled circuits CAD techniques to undergraduate students pursuing a degree course in electrical engineering or physics, and explains how a series of simulated experiments may be incorporated into the existing subjects. The simulated experiments make use of a two-dimensional open-access software based on the finite-element method. At the laboratory meetings, the students learn how to set up field problems for solution, and how to examine the results. Simulation tasks based on three axisymmetric open-boundary problems are used to introduce different numeric techniques to compute inductance and magnetic forces. The paper takes the reader to a step-by-step simulation journey, and provides all the basic elements required for further exploration of axially-symmetric systems.

关键词： Electromagnetic Engineering Education Finite Element Method Inductance Magnetic Forces Simulation Software

“Love Is Strong, and You＇re so Sweet”： JAGGER Is Essential for Persistent Synergid Degeneration and Polytubey Block in Arabidopsis thaliana

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Molecular Plant 2016年第4期9卷 601-614页

作者： Ana Marta pereira Margarida Sofia Nobre Sara Cristina Pinto Ana Lucia Lopes Mario Luis costa Simona Masiero Silvia Coimbra Departamento de Biologia Faculdade de Ciencias da Universidade do Porto Rua do Campo Alegre 4169-007 Porto Portugal Plant Functional Genomics Group Biosystems and Integrative Sciences Institute (BiolSI) 4169-007 Porto Portugal Dipartimento di BioScienze Universita degli Studi di Milano 20133 Milan Italy Present address: Institute of Plant Biology and ZUrich-Basel Plant Science Center University of ZUrich Zolikerstrasse 107 8008 Zurich Switzerland

Successful double fertilization and subsequent seed development in flowering plants requires the delivery of two sperm cells, transported by a pollen tube, into the embryo sac of an ovule. The embryo sac cells tightly control synergid cell death, and as a result the polyspermy block. Arabinogalactan proteins are highly glycosylated proteins thought to be involved in several steps of the reproductive process. We show that JAGGER, Arabinogalactan Protein 4, is an important molecule necessary to prevent the growth of multiple pollen tubes into one embryo sac in Arabidopsis thaliana. In jagger, an AGP4 knockout mutant, the pistils show impaired pollen tube blockage as a consequence of the survival of the persistent synergid. JAGGER seems to be involved in the signaling pathway that leads to a blockage of pollen tube attraction. Our results shed light on the mechanism responsible for preventing polyspermy in Arabidopsis and for safe- guarding successful fertilization of all ovules in one pistil, ensuring seed set and the next generation.

关键词： Polytubey persistent synergid arabinogalactan proteins polyspermy

Puff Models for Simulation of Fugitive Hazardous Emissions in Atmosphere

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Journal of Environmental Protection 2011年第2期2卷 154-161页

作者： Ledina Lentz pereira Camila Pinto da costa Marco Tullio Vilhena Tiziano Tirabassi 不详

A puff model for the dispersion of material from fugitive hazardous emissions is presented. For vertical diffusion the model is based on general techniques for solving time dependent advection-diffusion equation: the ADMM (Advection Diffusion Multilayer Method) and GILTT (Generalized Integral Laplace Transform Technique) techniques. Both approaches accept wind and eddy diffusion coefficients with any restriction in their height functions. Comparisons between values predicted by the models against experimental ground-level concentrations (from Copenhagen data set) are shown. The preliminary results confirm the applicability of the approaches proposed and are promising for future work.

关键词： Hazardous Emissions Advection-Diffusion Equation Analytical Solutions Air Pollution Modeling Puff Models