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Antioxidants Enzymes Activities and NO Levels in Hemodialysis and Continuous Ambulatory Peritoneal Dialysis—A Comparative Study

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Journal of Biosciences and Medicines 2024年第2期12卷 50-59页

作者： Natalia cristina Aparecida Ribeiro e Ribeiro Anderson Martins Tavares Jaslana Hainfellner da Silva João Paulo Lima Daher Salim Kanaan Luciene de carvalho Cardoso-Weide Laboratory of Neuroplasticity Neurobiology Department Biology Institute Federal Fluminense University Rio de Janeiro Brazil Postgraduate Program in Medical Sciences School of Medicine Federal Fluminense University Rio de Janeiro Brazil School of Biology UNIVERSO—Salgado de Oliveira University Rio de Janeiro Brazil department of Pathology Faculty of Medicine Federal Fluminense University Rio de Janeiro Brazil

It consists of a retrospective study with twenty-two individuals diagnosed with DRC: fourteen HD and eight CAPD, followed up in the dialysis center of Antonio Pedro University Hospital (HUAP) of fluminense Federal University (UFF), between 2015 and 2017. Fifteen healthy control (HC) subjects were included, without diagnosed CKD. Patients with HIV positive, hepatitis A, B, and C, pregnant, cancer, smokers, alcoholics and those exposed to X-rays in the last 3 months, were excluded. Objectives: As oxidative stress and endothelial dysfunction may be linked to the higher prevalence of CVD in CKD patients, we measured the activities of the antioxidants enzymes: SOD and GPx and total NO levels in the plasma and serum of end-stage CKD patients undergoing dialysis therapy, comparing with the HC group. Methods: Quantification of NO levels was performed by fluorometric kit, while activities of SOD and GPx were determined using kinetic methods. Results: We found higher plasma SOD activity in HD (8.58 U/ml) and CAPD (10.14 U/ml), compared to C (3.73 U/ml) group, while GPx activity was decreased in HD (115.38 nmol/h/ml) and CAPD (122.76 nmol/h/ml) groups compared to HC group (275.83 nmol/h/ml). Total serum NO concentration was decreased in HD (14.09 pmol/μl) and CAPD (10.26 pmol/μl), compared to non-CKD patients (49.65 pmol/μl). Conclusion: decreased total serum NO and GPx activities may lead to endothelial dysfunction and consequently a higher prevalence of CVD in CKD patients.

关键词： Hemodialysis CAPD SOD GPx NO

Antimicrobial and synergistic effects of lemongrass and geranium essential oils against Streptococcus mutans,Staphylococcus aureus,and Candida spp.

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World Journal of Critical Care Medicine 2024年第3期13卷 63-74页

作者： Patrícia Michelle Nagai de Lima Thaís Cristine Pereira Lara Steffany de carvalho Letícia Ferreira dos Santos Carlos Eduardo Rocha Oliveira Lucas de Paula Ramos Maria cristina Marcucci Amjad Abu Hasna Luciane Dias de Oliveira department of Biosciences and Oral Diagnosis Institute of Science and TechnologySão Paulo State UniversitySão Josédos Campos 12245000SPBrazil Anhembi Morumbi University School of MedicineSão Josédos Campos 12230-002SPBrazil department of Restorative dentistry Endodontics DivisionInstitute of Science and TechnologySão Paulo State UniversitySão Josédos Campos 12245000São PauloBrazil

BACKGROUND The oral cavity harbors more than 700 species of bacteria,which play crucial roles in the development of various oral diseases including caries,endodontic infection,periodontal infection,and diverse oral *** To investigate the antimicrobial action of Cymbopogon Schoenanthus and Pelargonium graveolens essential oils against Streptococcus mutans,Staphylococcus aureus,Candida albicans,***,and *** Minimum microbicidal concentration was determined following Clinical and Laboratory Standards Institute *** synergistic antimicrobial activity was evaluated using the Broth microdilution checkerboard method,and the antibiofilm activity was evaluated with the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide *** were analyzed by one-way analysis of variance followed by the Tukey post-hoc test(P≤0.05).RESULTS *** and *** essential oils were as effective as 0.12%chlorhexidine against *** and *** monotypic biofilms after 24 *** 24 h *** essential oil at 0.25%was more effective than the nystatin group,and *** essential oil at 0.25%was as effective as the nystatin *** *** and *** essential oils are effective against ***,***,***,***,and *** at different concentrations after 5 min and 24 h.

关键词： Antifungal effect Lemongrass Geranium Candida albicans Candida dubliniensis Candida krusei Staphylococcus aureus Streptococcus mutans

Bartter's syndrome:clinical findings,genetic causes and therapeutic approach

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World Journal of Pediatrics 2021年第1期17卷 31-39页

作者： flavia cristina carvalho mrad Sílvia Bouissou Morais Soares Luiz Alberto Wanderley de Menezes Silva Pedro Versiani dos Anjos Menezes Ana cristina Simoes-e-Silva Interdisciplinary Laboratory of Medical Investigation Faculty of MedicineFederal University of Minas Gerais(UFMG)Av.Alfredo Balena190Room#281Belo HorizonteMG 30130-100Brazil Pediatric Nephrology Unit Faculty of MedicineUFMGBelo HorizonteBrazil

Backgound Bartter's syndrome(BS)is a rare group of salt losing tubulopathies due to the impairment of transport mecha-nisms at the thick ascending limb of the Henle's *** sources Literature reviews and original research articles were collected from database,including PubMed and *** According to the time of onset and symptoms,BS can be classified into antenatal and classic *** studies have identified different subtypes of *** types Ⅰ,Ⅱ and Ⅲ are caused by mutations on genes encoding the luminal Na^(+)-K^(+)-2Cl^(-) co-transporter,the luminal K+ channel ROMK,and the basolateral chloride channel ClC-Kb(CLCNKB),***-of-function mutations of Barttin CLCNK type accessory beta subunit cause BS type Ⅳ*** mutations of CLCNKB and CLCNKA cause BS type Ⅳ*** type Ⅴ consists in a novel transient form characterized by antenatal presentation due to mutations in the MAGE family member *** gain-of-function mutations of the extracellular calcium sensing receptor gene can result in an autosomal dominant condition of *** clinical and biochemical alterations in BS include polyuria,dehydration,hypokalemia,hypochloremic metabolic alka-losis,hyperreninemia,high levels of prostaglandins,normal or low blood pressure,hypercalciuria and failure to *** focuses mainly at correcting dehydration and electrolyte disturbances and in measures to reduce polyuria,including the use of nonsteroidal anti-inflammatory medications to control excessive renal prostaglandin E2 *** Early diagnosis and treatment of BS may prevent long-term consequences such as growth failure,nephrocal-cinosis and end-stage renal disease.

关键词： Bartter's syndrome Hypercalciuria Hypokalemia Metabolic alkalosis Nephrocalcinosis

Cerium alleviates drought-induced stress in Phaseolus vulgaris

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Journal of Rare Earths 2020年第3期38卷 324-331页

作者： Olívia Graziela Gelioli Salgado Jéssica cristina Teodoro Joyce Pereira Alvarenga Cynthia de Oliveira Teotonio Soares de carvalho Débora Domiciano Paulo Eduardo Ribeiro Marchiori Luiz Roberto Guimaraes Guilherme department of Soil Science Federal University of LavrasCP 3037Campus UFLALavras37200-000Brazil department of Biology Federal University of LavrasCP 3037Campus UFLALavras37200-000Brazil

Extreme drought events are expected to be one of the main challenges for tropical agriculture and,consequently,for global food *** this reason,it is urgent to develop strategies to increase water use efficiency,as well as to increase crops’ ability to cope with *** rare earth elements(REEs) are known to alleviate damages in plants under abiotic stresses,we hypothesized that addition of Ce^3+to nutrient solution would promote the growth of common bean,especially under simulated drought *** test this hypothesis,we conducted an experiment evaluating the effect of six Ce^3+concentrations on the development and physiological traits of common bean grown in nutrient solution under normal condition and under induced drought stress,caused by the addition of polyethylene glycol6000 to the nutrient *** results show that Ce^3+alleviates water stress in common bean plants,increasing their survival rate and *** addition,Ce^3+application increases photosynthesis rate,chlorophyll content and water use efficiency under water ***,we observe no significant effect of Ce^3+on plants growing under normal ***,Ce3+seems to be a promising attenuator of drought stress for common bean.

关键词： Water stress Polyethylene glycol Rare earth elements Cerium nitrate Mitigation Common bean

Fraud investigation in commercial coffee by chromatography

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Food Quality and Safety 2018年第3期2卷 121-133页

作者： Víctor de carvalho Martins Ronoel Luiz de Oliveira Godoy Ana cristina Miranda Senna Gouvêa Manuela cristina Pessanha de Araujo Santiago Renata Galhardo Borguini Elaine cristina de Oliveira Braga Sidney Pacheco Luzimar da Silva de Mattos do Nascimento departamento de Tecnologia de Alimentos Instituto de TecnologiaUniversidade Federal Rural do Rio de JaneiroCEP 23890-000Seropédica-RJBrazil Laboratório de Cromatografia Líquida Embrapa Agroindústria de AlimentosCEP 23020-470Rio de Janeiro-RJBrazil Centro de Tecnologia Instituto de QuímicaUniversidade Federal do Rio de JaneiroCEP 22290-180Rio de Janeiro-RJBrazil

Coffee is currently the second largest commodity on the world market today,and there is great concern about the quality of the beans exported from producer countries to Europe and *** such as using blends of different species and adding low-cost raw materials,such as chicory,corn,and soybean,impair the sensory and functional characteristics of the drink made from roasted and ground coffee *** is a need to adopt more efficient analytical methods than the microscopy technique currently *** first chromatographic method used to determine fraud was reported in *** method used paper chromatography to differentiate between coffee and chicory based on the free reducing *** of the 1980s,different methods involving high-performance liquid chromatography and gas chromatography were developed in order to demonstrate geographic authenticity,distinction between species,occurrence of adulteration,and the presence of defective beans by determining the monosaccharides,oligosaccharides,tocopherols,fatty acids,volatiles,diterpenes,sterols,and phenolic substances,among *** far as the authors know,there are no papers published in the literature that have compiled such an extensive set of information about these chromatographic methods as *** the last 2 years,there has been a trend to develop analytical methods for ultra-performance liquid chromatography coupled with tandem mass spectrometry to confirm fraud in coffee,due to high sensitivity and selectivity.

关键词： adulteration coffee chromatographic methods carbohydrates volatile substances

dectin- 1 isoforms contribute to distinct Th 1/Th 17 cell activation in mucosal candidiasis

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Cellular & Molecular Immunology 2012年第3期9卷 276-286页

作者： Agostinho carvalho Gloria Giovannini AntoneUa de Luca Carmen D＇Angelo Andrea Casagrande Rossana G Iannitti Giovanni Ricci cristina Cunha Luigina Romani department of Experimental Medicine and Biochemical Sciences University of Perugia Perugia Italy Life and Health Sciences Research Institute （ICVS） School of Health Sciences University of Minho Braga Portugal CVS/3B＇s--PT Government Associate Laboratory Braga/Guimaraes Portugal

The recognition of p-glucans by dectin-1 has been shown to mediate cell activation, cytokine production and a variety of antifungal responses. Here, we report that the functional activity of dectin-1 in mucosal immunity to Candida albicans is influenced by the genetic background of the host. dectin-1 was required for the proper control of gastrointestinal and vaginal candidiasis in C57BIJ6, but not BALB/c mice; in fact, the latter showed increased resistance in the absence of dectin-1. The susceptibility of dectin-l-deficient C57BL/6 mice to infection was associated with defects in IL-17A and aryl hydrocarbon receptor-dependent IL-22 production and in adaptive Th I responses. In contrast, the resistance of dectin- 1-deficient BALB/c mice was associated with increased IL-17A and IL-22 production and the skewing towards Thl/Treg immune responses that provide immunological memory. Disparate canonical/ noncanonical NF-KB signaling pathways downstream of dectin-1 were activated in the two different mouse strains. Thus, the net activity of dectin-1 in antifungal mucosal immunity is dependent on the host＇s genetic background, which affects both the innate cytokine production and the adaptive Th1/Th17 cell activation upon dectin-1 signaling.

关键词： dectin-1 Mucosal candidiasis, Th1/Th17, 11-22, genetic background

New approach for obtaining the C-factor of RUSLE considering the seasonal effect of rainfalls on vegetation cover

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International Soil and Water Conservation Research 2021年第2期9卷 207-216页

作者： Pietro Menezes Sanchez Macedo Paulo Tarso Sanches Oliveira Mauro Antonio Homem Antunes Valdemir Lucio Durigon Elaine cristina Cardoso Fidalgo Daniel Fonseca de carvalho Agronomy-Soil Science Post Graduate Program Federal Rural University of Rio de JaneiroBR 465km 723897-000SeropedicaRJBrazil Federal University of Mato Grosso do Sul CxP.549Campo GrandeMS79070-900Brazil Federal Rural University of Rio de Janeiro Engineering DepartmentBR 465km 723897-000SeropedicaRJBrazil Technical College of the Federal Rural University of Rio de Janeiro BR 465km 723897-000SeropedicaRJBrazil National Center for Soil Research Brazilian Agricultural Research CorporationRio de JaneiroBrazil

We present a new approach for calculating the C-factor of RUSLE considering the effect of low-reflectance vegetation cover areas on the reduction of the effects on erosion caused by rainfall *** this,we propose the coefficients Cr2(rescaled 2)and C-PC(Precipitation Correction),which represent the Cfactor,and an adaptation in NDVI calculation,according to the seasonality of precipitation(NDVI-PC).The Cr2 factor is used when there is no seasonal effect of rainfall on vegetation,while the C-PC factor is calculated for localities under the influence of seasonality,from *** proposed approaches were tested using different satellites images in the Palmares-Ribeir~ao do Saco watershed,Rio de Janeiro,*** values of Cr2 and C-PC factors were compared to the Cr factor(rescaled)and to mean values from the literature for different land *** results indicated that the Cr2 factor represents an improvement in accuracy in relation to Cr by considering specific values of the studied area to normalize the data without ***,the C-PC factor is able to simulate the effect of seasonality,providing more realistics values of soil loss by the RUSLE as a function of the proportion of area affected by the rainfall seasonality obtained from *** conclude that both Cr2 and C-PC factors generate values similar of the C-factor observed in the literature,and therefore are able to provide better soil loss estimation than that using the Cr factor.

关键词： Soil erosion Land cover Soil use and management factor Vegetation seasonality NDVI

Non-hematopoietic cells contribute to protective tolerance to Aspergillus fumigatus via a TRIF pathway converging on IDO

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Cellular & Molecular Immunology 2010年第6期7卷 459-470页

作者： Antonella de Luca Silvia Bozza Teresa Zelante Silvia Zagarella Carmen D’Angelo Katia Perruccio Carmine Vacca Agostinho carvalho cristina Cunha Franco Aversa Luigina Romani department of Experimental Medicine and Biochemical Sciences University of PerugiaPerugiaItaly Life and Health Sciences Research Institute(ICVS) School of Health SciencesUniversity of MinhoBragaPortugal

Innate responses combine with adaptive immunity to generate the most effective form of anti-Aspergillus immune *** the pivotal role of dendritic cells in determining the balance between immunopathology and protective immunity to the fungus is well established,we determined that epithelial cells(ECs)also contributes to this ***,EC-mediated protection occurred through a Toll-like receptor 3/Toll/IL-1 receptor domain-containing adaptor-inducing interferon(TLR3/TRIF)-dependent pathway converging on indoleamine 2,3-dioxygenase(IDO)via non-canonical nuclear factor-kB *** with the high susceptibility of TRIF-deficient mice to pulmonary aspergillosis,bone marrow chimeric mice with TRIF unresponsive ECs exhibited higher fungal burdens and inflammatory pathology than control mice,underexpressed the IDO-dependent T helper 1/regulatory T cell(Th1/Treg)pathway and overexpressed the Th17 pathway with massive neutrophilic inflammation in the *** studies with interferon(IFN)-c,IDO or IL-17R unresponsive cells confirmed the dependency of immune tolerance to the fungus on the IFN-c/IDO/Treg pathway and of immune resistance on the MyD88 pathway controlling the fungal ***,distinct immune pathways contribute to resistance and tolerance to the fungus,to which the hematopoietic/non-hematopoietic compartments contribute through distinct,yet complementary,roles.

关键词： aspergillosis epithelial cells IDO Th17/Treg transplantation tolerance

Prevalence of Human Leukocyte Antigen HLA-B*5701 in HIV-1 Infected Individuals in Brazil

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Open Journal of Genetics 2014年第1期4卷 56-62页

作者： Claudinéia de Araújo cristina Valetta de carvalho Miriam Estela de Souza Freire Amanda Yamaguti Ivens Cuiabano Scaff Fernando José de Souza flávia Galindo Silvestre Silva Ricardo Sobhie Diaz Ismael Dale Cotrim Guerreiro da Silva department of Gynecology Molecular Gynecology Laboratory Federal University of S&atildeo Paulo S&atildeo Paulo Brazil State department of Health of Mato Grosso Cuiabá Brazil Unirondon University Cuiabá Brazil Infectious Diseases Division Federal University of Mato Grosso Cuiabá Brazil Imunogenetics Laboratory General University Hospital Cuiabá Brazil

This study was designed to establish the prevalence of HLA-B*5701 at HIV-1 infected individuals in Brazil. A total of 517 consecutive individuals were followed in this study from February 2009 through July 2011. The presence of HLA-B*5701 was determined by Nested-PCR with HLA-B*57 and HLA-B*5701 sequence-specific primers (PCR-SSP). The expression of HLA-B*57 was negative in the 385 (74.5%) and positive in the 103 (19.9%) of infected individuals. Among these, the expression of HLA-B5701 was positive in the 29 (5.6%) of individuals. No demographic or ethnic differences were found between HLA-B*57/HLA-B*5701 HIV-1 negative patients, with a prevalence of Caucasians (57.1%) individuals. During the period of study, 68 patients were submited to an abacavir contain- ing regimen. The HLA-B*5701 allele was observed in 7 (10.3%) patients, with a significant incidence of Hypersensitivity reactions at 4 of them (p < 0.001). Conclusions: Although Brazilian population consists of a mixture of individuals of Caucasian, African and Native American genetic background, prevalence of HLA-B*5701 in this population is similar to the one found in pure Caucasians.

关键词： Immunogenetics Human Immunodeficiency Virus HLA-B*5701 Antigen Human Abacavir