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Food and Nutrition Sciences 2014年第21期5卷 2053-2062页

作者： Florinda Fratianni Rosa Pepe filomena nazzaro Istituto di Scienze dell’Alimentazione Consiglio Nazionale delle Ricerche (ISA-CNR) Via Roma Italy Experimental Institute for Vegetable Crops—CRA Pontecagnano Italy

Biochemical characteristics, antimicrobial and quorum quenching activity of the extract of the “Carciofo di Montoro”, a typical ecotype of Cynara cardunculus var. scolymus of the Campania region (Southern Italy) were studied, to consider it as potential reserve of bioactive constituents useful for food industry and beneficial for managing and preventing several chronic illnesses in humans. The extract exhibited a good polyphenol content (528 μg GAE/g) and antioxidant activity (EC50 less than 5 mg). Ultra pressure liquid chromatography (UPLC) revealed high amount of chlorogenic acid, cynarin and epicatechin. The extract showed antimicrobial activity against Escherichia coli, Staphylocccus aureus, Pseudomonas aeruginosa, Enterococcus faecalis and Bacillus cereus pathogen strains. Finally, quorum quenching activity was demonstrated. The variety Carciofo di Montoro could represent a good source of health-promoting polyphenols, encouraging a nutraceutical use of such ecotype, for several phyto-pharmaceutical applications.

关键词： Artichoke Polyphenols Antioxidant Antimicrobial Quorum Sensing

Polyphenol Composition and Antioxidant Activity of Different Potentially Functional Kale-Based Snacks

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Food and Nutrition Sciences 2014年第12期5卷 1145-1152页

作者： filomena nazzaro Federica Cardinale Autilia Cozzolino Tiziana Granese Florinda Fratianni Institute of Food Science ISA-CNR Avellino Italy

Four types of kale-based snacks were investigated on the basis of polyphenol content and composition, glucosinolates amount, and antioxidant activity. The amount of polyphenols resulted to be higher than that of raw material, fresh kale (0.57 ± 0.03 mg GAE). The antioxidant activity turned out indeed very effective, resulting sufficient just from 0.72 mg to 2.1 mg to dishearten the activity of 1 ml DPPH to 50%. Glucosinolates present in kale-based snacks ranged from 1.17 mg/gr to 1.52 mg of sinigrin equivalent/gr. Polyphenol profile, obtained by UPLC-DAD analysis allowed us to identify gallic acid, chlorogenic acid, and catechin common in all samples. Other polyphenols, that is caffeic acid, p-coumaric acid, and ferulic acid, were found in almost all samples, almost all more abundant than fresh kale, representing a precious source of beneficial metabolites. Our results suggest that such snacks could represent an effective functional dietary supplement also if compared to fresh vegetable. Manufacturing of kale-based snacks does not negatively affect the beneficial effect of the raw material;indeed, such snacks could positively meet people’s expectation, mainly children and young, well known consumers of snacks, which, also in such manner, could be addressed towards a healthier dietary regime.

关键词： Kale Antioxidant Polyphenols Glucosinolates UPLC

Variation of Polyphenols, Anthocyanins and Antioxidant Power in the Strawberry Grape (Vitis labrusca) after Simulated Gastro-Intestinal Transit and Evaluation of in VitroAntimicrobial Activity

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Food and Nutrition Sciences 2014年第1期5卷 60-65页

作者： Tiziana Granese Federica Cardinale Autilia Cozzolino Selenia Pepe Maria Neve Ombra filomena nazzaro Raffaele Coppola Florinda Fratianni Institute of Food Science ISA-CNR Avellino Italy Institute of Food Science ISA-CNR Avellino Italy.

The influence of a simulated digestive process on some biochemical and biological aspects of strawberry grape (Vitis labrusca) was investigated. The amount of total polyphenols and anthocyanins as well as the antioxidant power were evaluated. Results evidenced that the simulated gastrointestinal transit caused a decrease of the polyphenols content and total anthocyanins;these last, however, were more resistant than polyphenols, decreasing only of 50% respect to the initial value (31.50 μg/ml of extract). The extract exhibited an excellent antioxidant power (EC50 3.8 mg/ml), which decreased of about four times after the simulated gastrointestinal transit. The antimicrobial activity of the extract, evaluated against three Escherichia coli, Pseudomonas aeruginosa and Bacillus cereus pathogen strains was enhanced by the simulated digestion, with an increase of the inhibition halo.

关键词： Strawberry Grape Antioxidants Polyphenols Anthocyanins Antimicrobial Activity

Polyphenol Composition and Antioxidant Activity of Two Autochthonous Brassicaceae of the Campania Region, Southern Italy

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Food and Nutrition Sciences 2014年第1期5卷 66-70页

作者： Florinda Fratianni Federica Cardinale Autilia Cozzolino Tiziana Granese Selenia Pepe Riccardo Riccardi Patrizia Spigno Raffaele Coppola filomena nazzaro Cooperative ARCA Acerra Italy Institute of Food Science ISA-CNR Avellino Italy Institute of Food Science ISA-CNR Avellino Italy

Torzella (Brassica oleracea acephala) is one of the most ancient kinds of cauliflower developed in the Mediterranean area. Broccolo (Brassica oleracea botrytis cimosa), var. “San Pasquale” is a leafy vegetable cultivated in Campania too, mainly in the province of Naples. We evaluated the polyphenols content and the antioxidant activity of these two Brassicaceae, provided by the same experimental plant of the Campania region. Both vegetables showed high content of total polyphenols (6.37 mM GAE/g and 2.24 mM GAE/g of Torzella and Broccolo, respectively), and a remarkable antioxidant activity (EC50 1.53 mg and EC50 6.51 mg, in Torzella and Broccolo, respectively). Polyphenol composition, determined by ultra performance liquid chromatography (UPLC), allowed us to detect a certain number of compounds (gallic, ferulic, chlorogenic, p-coumaric and caffeic acids, catechin, luteolin, naringenin) common to both species. Therefore, epicatechin was found only in the extract of san Pasquale broccoli;on the contrary, rutin and apigenin were detected only in the extracts of torzella broccoli. Hence, such products can be considered therapeutic functional foods due to their extraordinary reserve of secondary metabolites and bioactive constituents that are beneficial for managing and preventing several chronic illnesses in humans.

关键词： Brassicaceae Antioxidants Polyphenols