检索结果-南通市图书馆

Food and Nutrition Sciences 2014年第1期5卷 60-65页

作者： Tiziana Granese Federica Cardinale Autilia Cozzolino Selenia Pepe Maria Neve Ombra filomena Nazzaro Raffaele Coppola Florinda Fratianni Institute of Food Science ISA-CNR Avellino Italy Institute of Food Science ISA-CNR Avellino Italy.

The influence of a simulated digestive process on some biochemical and biological aspects of strawberry grape (Vitis labrusca) was investigated. The amount of total polyphenols and anthocyanins as well as the antioxidant power were evaluated. Results evidenced that the simulated gastrointestinal transit caused a decrease of the polyphenols content and total anthocyanins;these last, however, were more resistant than polyphenols, decreasing only of 50% respect to the initial value (31.50 μg/ml of extract). The extract exhibited an excellent antioxidant power (EC50 3.8 mg/ml), which decreased of about four times after the simulated gastrointestinal transit. The antimicrobial activity of the extract, evaluated against three Escherichia coli, Pseudomonas aeruginosa and Bacillus cereus pathogen strains was enhanced by the simulated digestion, with an increase of the inhibition halo.

关键词： Strawberry Grape Antioxidants Polyphenols Anthocyanins Antimicrobial Activity

CAN STEP EXERCISE PREVENT GAIT IMPAIRMENTS IN ELDERLY WOMEN? A KINETIC ANALYSIS

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

CAN STEP EXERCISE PREVENT GAIT IMPAIRMENTS IN ELDERLY WOMEN?...

Penicillin-Resistant Aerococcus viridansBacteremia Associated with Bovine Severe Respiratory Syndrome

第23届国际运动生物力学学术年会

作者： Maria Lourdes Machado Hel Isa André Rita Santos-Rocha António Veloso filomena Carnide Faculty of Human Kinetics Technical University of Lisbon

This research aimed to investigate lower limb motion while a subject negotiated a raised surface to study the effects of age-related declines in *** selected foot-ground force variables to highlight possible differences between the young and elder *** gait of ten post-menopause women that took part in a step exercise program and ten skilled young female were analyzed with a two force-platforms during an unobstructed walking and when subjects were stepping on and off a raised surface of 17.5 cm *** results suggest that older subjects possess GRF patterns similar to youngsters during unobstructed *** can be concluded that senior step exercise programs are capable to prevent gait impairments in the elderly but this positive effect is lessened in the case of more demanding walking tasks which should be taken into account in the development of senior step exercise programs.

关键词： elderly gait GRF kinetics fitness programs

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Open Journal of Veterinary Medicine 2013年第2期3卷 131-135页

作者： Jacopo Guccione Sandra Nizza Karina Mallardo Antonietta Cantiello filomena Fiorito Antonio Di Loria Luisa De Martino Department of Pathology and Animal Health Infectious Diseases Section Faculty of Veterinary Medicine University of Naples “Federico II” Naples Italy

Aerococcus viridans, a less frequently isolated bacteria, is a gram-positive, catalase-negative coccus, found singly or in tetrads, with biochemical and growth characteristics of streptococci and enterococci. This microorganism, usually susceptible to penicillin, is often found in the environment and is infrequently associated with human/veterinary infections. We described a case of Holstein Friesian female calf, 150-day-old, affected by respiratory emergencies. Following the clinical signs, radiographic analysis and bacteriological/molecular examinations carried out on blood culture, a diagnosis of severe broncho-pulmonary disease associate with a multidrug-resistant A. viridans bacteremia was done. The present case highlights the invasive nature of a saprophytic bacterium showing a broad profile of antibiotic-resistance including β-lactams. Furthermore, this report confirms that the effectiveness of an antibiotic therapy is based primarily on a sure diagnosis including susceptibility testing.

关键词： Respiratory Syndrome Aerococcus viridans Antibiotic-Resistance

Down-Regulation of GKN1 in Gastric Cancer Is Not Associated with the RUNX3 Expression

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Biosciences and Medicines 2017年第11期5卷 80-90页

作者： Chiara Stella Di Stadio filomena Altieri Antonella Federico Giuseppina Miselli Antonello Niglio Maurizio De Palma Emilia Rippa Paolo Arcari Department of Molecular Medicine and Medical Biotechnology University of Naples Federico II Naples Italy Hospital A. Cardarelli Naples Italy CEINGE Advanced Biotechnology Scarl Naples Italy

Gastrokine 1 (GKN1) is a gastric mucosal protein highly expressed and secreted in normal individuals but during Helicobacter pylori infection or in gastric carcinogenesis it is strongly down-regulated or totally absent. In gastric cancer, the GKN1 gene is silenced through an epigenetic mechanism most likely mediated by a transcription factor that promotes on GKN1 promoter the activity of the enzymes SUV39H1 and HDACs. Because RUNX3 is a potential candidate in the regulation of molecular carcinogenesis process of stomach cancers, we tried to assess if RUNX3 could be involved in GKN1 down-regulation in GC. 17 paired of non-tumoral and tumoral surgical specimens from patients with gastric cancer were analyzed for GKN1 and RUNX3 by Western blotting and chromatin immunoprecipitation (Chip) assays. The overall results indicated that RUNX3 expression was not associated with the down-regulation of GKN1. The expression levels of RUNX3 in non-tumoral and tumoral samples suggest that RUNX3 does not act as a tumor suppressor but that it might play a complex oncogenic role in gastric cancer cells.

关键词： Gastrokine 1 Gastric Cancer Epigenetics RUNX3 Tumor Suppressor

Polyphenol Composition and Antioxidant Activity of Two Autochthonous Brassicaceae of the Campania Region, Southern Italy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food and Nutrition Sciences 2014年第1期5卷 66-70页

作者： Florinda Fratianni Federica Cardinale Autilia Cozzolino Tiziana Granese Selenia Pepe Riccardo Riccardi Patrizia Spigno Raffaele Coppola filomena Nazzaro Cooperative ARCA Acerra Italy Institute of Food Science ISA-CNR Avellino Italy Institute of Food Science ISA-CNR Avellino Italy

Torzella (Brassica oleracea acephala) is one of the most ancient kinds of cauliflower developed in the Mediterranean area. Broccolo (Brassica oleracea botrytis cimosa), var. “San Pasquale” is a leafy vegetable cultivated in Campania too, mainly in the province of Naples. We evaluated the polyphenols content and the antioxidant activity of these two Brassicaceae, provided by the same experimental plant of the Campania region. Both vegetables showed high content of total polyphenols (6.37 mM GAE/g and 2.24 mM GAE/g of Torzella and Broccolo, respectively), and a remarkable antioxidant activity (EC50 1.53 mg and EC50 6.51 mg, in Torzella and Broccolo, respectively). Polyphenol composition, determined by ultra performance liquid chromatography (UPLC), allowed us to detect a certain number of compounds (gallic, ferulic, chlorogenic, p-coumaric and caffeic acids, catechin, luteolin, naringenin) common to both species. Therefore, epicatechin was found only in the extract of san Pasquale broccoli;on the contrary, rutin and apigenin were detected only in the extracts of torzella broccoli. Hence, such products can be considered therapeutic functional foods due to their extraordinary reserve of secondary metabolites and bioactive constituents that are beneficial for managing and preventing several chronic illnesses in humans.

关键词： Brassicaceae Antioxidants Polyphenols

Efflux Pumps Modulation in Colorectal Adenocarcinoma Cell Lines: The Role of Nuclear Medicine

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Cancer Therapy 2011年第3期2卷 408-417页

作者： Joao Casalta-Lopes Ana Margarida Abrantes Mafalda Laranjo Joana Rio Ana Cristina Goncalves Barbara Oliveiros Ana Bela Sarmento-Ribeiro Maria filomena Botelho Biophysics Unit IBILIFaculty of MedicineUniversity of CoimbraCoimbraPortugal Centre of Investigation on Cancer Envi-ronmentGenetics and OncobiologyFaculty of MedicineUniversity of CoimbraCoimbraPortugal Institute of Nuclear Sciences Applied to Health University of CoimbraCoimbraPortugal Applied Molecular Biology Unit Faculty of MedicineUniversity of CoimbraCoimbraPortugal.

Introduction: Multidrug resistance (MDR) is one of the major problems of chemotherapy. Overexpression of efflux pumps, such as P-glycoprotein (Pgp), multiple resistance-related protein 1 (MRP-1) and lung resistance protein (LRP) can lead to MDR. Verapamil and L-buthionine-sulfoximine (BSO) are two modulators of these proteins. This study aims to compare 99mTc-Sestamibi transport kinetics in human colorectal adenocarcinoma cell lines, in the presence and absence of the MDR modulators verapamil and BSO. Material and Methods: MDR proteins expression was evaluated in sensitive (WiDr) and resistant (LS1034) human colorectal adenocarcinoma cell lines. Intracellular and plasma membrane Pgp and MRP1, and LRP expression was analyzed by flow-cytometry and western blot. Cellular transport kinetics was assessed using 99mTc-Sestamibi. MDR modulation was evaluated though retention studies in resistant cells after incubation with the modulators. Results: Pgp expression was significantly higher (p≤0.001) in resistant cells. These results were confirmed by western blot analysis. 99mTc-Sestamibi uptake and retention percentage were significantly higher (p99mTc-Sestamibi, there were differences among the MDR modulators used (p99mTc-Sestamibi could indicate MDR phenotype in colorectal adenocarcinoma cells. As the modulators used showed a reversion of the retention profile only for the first minutes, their administration should occur immediately before the administration of cytotoxic drugs.

关键词： Multidrug Resistance P-Glycoprotein Multidrug Resistance-Associated Protein 1 Major Vault Protein

Bark Stripping from Forest Tree Species in Madjadjane, Southern Mozambique: Medicinal Uses and Implications for Conservation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Natural Resources 2014年第5期5卷 192-199页

作者： Annae M. Senkoro filomena M. A. Barbosa Sharmila F. Moiane Gabriel Albano Ana I. Ribeiro de Barros Faculty of Agronomy UEM Maputo Mozambique Faculty of Sciences Eduardo Mondlane University (UEM) Maputo Mozambique Tropical Research Institute Oeiras Portugal Biotechnology Center UEM Maputo Mozambique

Tree bark is one of the most important non-timber forest products. In less developed countries, it is used for multiple purposes, particularly in traditional medicine. This paper addresses the question of bark exploitation, uses, and impacts in Madjadjane village, southern Mozambique. For that, we have conducted an ethnobotanical survey and analysed the level of damage of the ten most exploited tree species. Bark was mainly used for medical purposes, spanning 13 different applications. Most of the species had more than one medical application constituting potential sources of valuable biocompounds. In general the level of damage caused by debarking was not critical, but should be seen with caution. An upgrade and update of the results will be of utmost importance to estimate with more accuracy the current conservation status as well as to predict future impacts and define better conservation strategies. We suggest the expansion of ethnobotanical surveys as well as their integration in broad programs aimed at the preservation and valorization of local heritage. This will encourage equitable access and benefit sharing of biodiversity as well as the promotion of bio-based economy.

关键词： Bark Ethnobotany Traditional Knowledge Conservation Mozambique

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

壳聚糖基皮革抗菌涂层的研究

中国皮革 2014年第3期43卷 48-50页

作者： Isabel P.Fernandes Joana S.Amaral Vera Pinto Maria José Ferreira Maria filomena Barreiro 分离与反应工程实验室(LSRE) 副实验室LSRE/LCM布拉干萨理工学院葡萄牙布拉干萨5301857 药剂学院波尔图大学葡萄牙波尔图4050313 布拉干萨理工学院葡萄牙布拉干萨5301857 葡萄牙制鞋技术中心葡萄牙圣若昂3700121

自抗菌类涂料在鞋材中应用以来,引起了制造商和消费者的极大兴趣。研究利用壳聚糖固有的抗菌活性和成膜性制备皮革抗菌材料。考虑到皮革鞣制的特殊性,采用不同的涂饰技术,如转鼓浸渍、辊涂和喷涂。结果表明,采用转鼓浸渍的方法效果最佳... 详细信息

自抗菌类涂料在鞋材中应用以来,引起了制造商和消费者的极大兴趣。研究利用壳聚糖固有的抗菌活性和成膜性制备皮革抗菌材料。考虑到皮革鞣制的特殊性,采用不同的涂饰技术,如转鼓浸渍、辊涂和喷涂。结果表明,采用转鼓浸渍的方法效果最佳。进一步研究了转鼓浸渍涂饰方法中壳聚糖的用量、增溶酸剂的类型和浸渍时间,对抗菌剂抗菌能力的影响。由于壳聚糖价格昂贵(成本原因)以及对最终试验结果(采用SEM考察了抗菌能力和成膜效果)的分析,得出最佳方案:壳聚糖用量为1%(w/v),溶剂为甲酸,转鼓浸渍时间为2h。

关键词：壳聚糖皮革鞋类抗菌涂料

Sustained virological response: A milestone in the treatment of chronic hepatitis C

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2013年第18期19卷 2793-2798页

作者： filomena Morisco Rocco Granata Tommaso Stroffolini Maria Guarino Laura Donnarumma Laura Gaeta Ilaria Loperto Ivan Gentile Francesco Auriemma Nicola Caporaso Dipartimento di Medicina Clinica e Chirurgia Gastroenterology Unit University of Naples "Federico Ⅱ" 80131 Naples Italy Dipartimento di Malattie Infettive e Tropicali University of Rome "La Sapienza" 00161 Rome Italy Dipartimento di Medicina Clinica e Chirurgia Infectious Diseases Unit University of Naples "Federico Ⅱ" 80131 Naples Italy

AIM: To evaluate the long-term eradication of hepatitis C virus (HCV) infection and liver-related complications in chronically infected patients that have achieved sustained virological response. METHODS: One hundred and fifty subjects with chronic hepatitis C (CHC) or cirrhosis and sustained virological response (SVR) between the years of 1989 and 2008 were enrolled in a long-term clinical follow-up study at the Gastrointestinal and Liver Unit of the University Hospital of Naples "Federico Ⅱ". At the beginning of the study, the diagnosis of HCV infection was made on the basis of serum positivity for antibodies to HCV and detection of HCV RNA transcripts, while a diagnosis of chronic hepatitis was formulated using imaging techniques and/or a liver biopsy. SVR was achieved by interferon-based therapy, both conventional and pegylated, with and without ribavirin treatment. The patients were evaluated for follow-up at a median length of 8.6 years, but ranged from 2-19.9 years. Among them, 137 patients had pre-treatment CHC and 13 had cirrhosis. The patients were followed with clinical, biochemical, virological, and ultrasound assessments on a given schedule. Finally, a group of 27 patients underwent a liver biopsy at the beginning of the study and transient elastography at their final visit to evaluate changes in liver fibrosis. RESULTS: The median follow-up was 8.6 years (range 2-19.9 years). HCV RNA remained undetectable in all patients, even in patients who eventually developed liver-related complications, indicating no risk of HCV recurrence. Three liver-related complications were observed: two cases of hepatocellular carcinoma and one case of bleeding from esophageal varices resulting in an incidence rate of 0.23%/person per year. Further, all three complications took place in patients diagnosed with cirrhosis before treatment began. Only one death due to liver-related causes occurred, resulting in a mortality rate of 0.077% person per year. This amounts to a 99.33

关键词： Antiviral therapy Cirrhosis Hepatitis C virus Sustained virological response Fibrosis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

壳聚糖基皮革抗菌涂层的研究(续)

中国皮革 2014年第5期43卷 49-53页

作者： Isabel P. Fernandes Joana S. Amaral Vera Pinto Maria José Ferreira Maria filomena Barreiro 陈小珂分离与反应工程实验室(LSRE)副实验室LSRE/LCM布拉干萨理工学院葡萄牙布拉干萨5301857 药剂学院波尔图大学葡萄牙波尔图4050313 布拉干萨理工学院葡萄牙布拉干萨5301857 葡萄牙制鞋技术中心葡萄牙圣若昂3700121

关键词：壳聚糖皮革鞋类抗菌涂料