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Stage-Specific Changes on Plasmodium yoelii yoelii Following Treatment with Hintonia latifloraStem Bark Extract and Phytochemical-Antioxidant Evaluation

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Pharmacology & Pharmacy 2017年第12期8卷 381-395页

作者： Elba Carrasco-Ramírez Perla Y. López-Camacho Armando Zepeda-Rodríguez Patricia Bizarro-nevares filiberto malagón-gutiérrez Gustavo Basurto-Islas norma Rivera-Fernández Laboratorio de Malariología Departamento de Microbiología y Parasitología Facultad de Medicina Universidad Nacional Autónoma de México CDMX México Departamento de Ciencias naturales e Ingeniería Universidad Autónoma Metropolitana CDMX México Deparatmento de Biología Celular y Tisular Facultad de Medicina Universidad Nacional Autónoma de México CDMX México Departamento de Ingenierías Química Electrónica y Biomédica División de Ciencias e Ingeniería Universidad de Guanajuato Campus León Guanajuato México

Malaria endemic zones are mostly located on third world countries, where antimalarials are not easily found or patients cannot afford them, and in consequence, they must turn toward natural products or phytomedicines. In the present study, the effect of Hinotnia latiflora (Hl) methanolic stem bark extract (HlMeOHe) on the ultrastructure of the asexual intraerythrocytic stages of Plasmodium yoelii yoelii (Pyy) after a Peters’ four-day oral treatment was assessed by transmission electron microscopy (TEM), as well as the parasite development on blood smears, analyzed by light microscopy. Likewise, extract was subjected to qualitative tests adopting standard procedures for identification of phytoconstituents;its antioxidant activity was evaluated according to the method of Brand-Williams and by the radical cation decolorization assay. Results showed higher percentage of rings and lower percentage of trophozoites and schizonts in the treated animals, in comparison with those of the control groups, which demonstrated lower percentage of rings and trophozoites, and schizonts in higher number. Images of TEM showed in some treated parasites, mild parasite membranes, organelle swelling and ribosomal depletion. The phytochemical profile demonstrated that the extract contains alkaloids, tannis, steroids, terpenoids, flavonoids, phenolics and saponins. The obtained values of the half maximal inhibitory concentration (IC50) in μg/mL, for both antioxidant assays were of 423.83 and 202.95 respectively. It is concluded that HlMeOHe altered the development of the intraerythrocytic asexual stages and the ultrastructure of Pyy, and due to its phytochemical constituents, showed an in vitro antioxidant activity.

关键词： Hintonia latiflora Plasmodium yoelii yoelii Malaria Ultrastructural Changes Phytochemicals Antioxidant natural Products

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Use of a combination strategy to improve neuroprotection and neuroregeneration in a rat model of acute spinal cord injury

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neural Regeneration Research 2019年第6期14卷 1060-1068页

作者： Elisa García Roxana Rodríguez-Barrera Vinnitsa Buzoianu-Anguiano Adrian Flores-Romero Emanuel malagón-Axotla Marco Guerrero-Godinez Estefanía De la Cruz-Castillo Laura Castillo-Carvajal Monserrat Rivas-Gonzalez Paola Santiago-Tovar Ivis Morales Cesar Borlongan Antonio Ibarra Centro de Investigación en Ciencias de la Salud (CICSA) Facultad de Ciencias de la SaludUniversidad Anáhuac México Campus Norte Centro de Investigación del Proyecto CAMInA A.C. Unidad de Investigación Médica en Enfermedades neurologicas Hospital Especialidades CMN Siglo XXI Unidad de Rehabilitación Osteoarticular.Instituto nacional de Rehabilitación. Luis Guillermo Ibarra Ibarra Center of Excellence for Aging and Brain Repair Department of Neurosurgery and Brain RepairUniversity of South Florida Morsani College of Medicine

Spinal cord injury is a very common pathological event that has devastating functional consequences in patients. In recent years, several research groups are trying to find an effective therapy that could be applied in clinical practice. In this study, we analyzed the combination of different strategies as a potential therapy for spinal cord injury. Immunization with neural derived peptides(InDP), inhibition of glial scar formation(dipyridyl: DPY), as well as the use of biocompatible matrix(fibrin glue: FG) impregnated with bone marrow mesenchymal stem cells(MSCs) were combined and then its beneficial effects were evaluated in the induction of neuroprotection and neuroregeneration after acute SCI. Sprague-Dawley female rats were subjected to a moderate spinal cord injury and then randomly allocated into five groups: 1) phosphate buffered saline; 2) DPY; 3) InDP + DPY; 4) DPY+ FG; 5) InDP + DPY + FG + MSCs. In all rats, intervention was performed 72 hours after spinal cord injury. Locomotor and sensibility recovery was assessed in all rats. At 60 days after treatment, histological examinations of the spinal cord(hematoxylin-eosin and Bielschowsky staining) were performed. Our results showed that the combination therapy(DPY+ InDP + FG+ MSCs) was the best strategy to promote motor and sensibility recovery. In addition, significant increases in tissue preservation and axonal density were observed in the combination therapy group. Findings from this study suggest that the combination theapy(DPY+ InDP + FG + MSCs) exhibits potential effects on the protection and regeneration of neural tissue after acute spinal cord injury. All procedures were approved by the Animal Bioethics and Welfare Committee(approval no. 178544; CSnBTBIBAJ 090812960)on August 15, 2016.

关键词： fibrin glue mesenchymal stem cells glial scar protective autoimmunity neural derived peptides neural regeneration

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Recurrence of inflammatory pseudotumor in the distal bile duct: Lessons learned from a single case and reported cases

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世界胃肠病学杂志：英文版 2006年第24期12卷 3938-3943页

作者： EM López-Tomassetti Fernández H Díaz Luis A Martín malagón I Arteaga González A Carrillo Pallarés Department of Gastrointestinal Surgery University Hospital of Canary Islands Ofra s/n. La Cuesta38320 La LagunaSanta Cruz de TenerifeSpain

煽动性的 myofibroblastic 肿瘤(IMT ) 或煽动性的假肿瘤(IP ) 广泛地在文学被讨论了。他们通常在肺和上面的呼吸道被发现。然而，包含 biliopancreatic 区域的案例报导在最近的年增加了。限制到胰腺的头或远侧的胆汁管的这些损害的 Imm... 详细信息

煽动性的 myofibroblastic 肿瘤(IMT ) 或煽动性的假肿瘤(IP ) 广泛地在文学被讨论了。他们通常在肺和上面的呼吸道被发现。然而，包含 biliopancreatic 区域的案例报导在最近的年增加了。限制到胰腺的头或远侧的胆汁管的这些损害的 Immunohistochemical 学习似乎与那些在称为自体免疫的胰腺炎的一个新实体观察了兼容，但是通常强烈的纤维变性反应(带状配列) 支配生产一个团。当这个条件限于胰腺的头时，胆总管可能被煽动性的过程包含，黄疸可以经常发生类似于胰的腺癌。我们以前报导了从与是一个极其稀罕的实体的自体免疫的胰腺炎联系的胆汁管产生的 IMT 的一个案例。在 Kaush-Whipple 切除术以后的四年，平淡的后续上的放射学的检查揭示了一个肿瘤团，建议本地复发。指导超声的 FnA 证实了我们的可疑诊断。作为其它，这个现在的案例建议坚持的后续是必要的以便在这个特定的地点阻止不可逆的肝损坏。

关键词：炎症假瘤胆汁疾病成肌纤维细胞临床

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Intestinal pseudo-obstruction in patients with systemic lupus erythematosus: A real diagnostic challenge

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World Journal of Gastroenterology 2014年第32期20卷 11443-11450页

作者： Carlos Alberto García López Fernando Laredo-Sánchez José malagón-Rangel Miguel G Flores-Padilla Haiko nellen-Hummel Department of Internal Medicine Specialities Hospital National Medical Centre "Siglo XXI" Mexican Social Security Institute Distrito Federal 06720 México

Intestinal pseudo-obstruction secondary to systemic lupus erythematosus(SLE) is a rare syndrome described in recent decades. There are slightly over 30 published cases in the English language literature, primarily associated with renal and hematological disease activity. Its presentation and evolution are a diagnostic challenge for the clinician. We present four cases of intestinal pseudo-obstruction due to lupus in young Mexican females. One patient had a previous diagnosis of SLE and all presented with a urinary tract infection of varying degrees of severity during their evolution. We consider that recognition of the disease is of vital importance because it allows for establishing appropriate management, leading to a better prognosis and avoiding unnecessary surgery and complications.

关键词： Intestinal pseudo-obstruction Systemic lupus eryth

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Mass-forming pancreatitis: Value of contrast-enhanced ultrasonography

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World Journal of Gastroenterology 2006年第26期12卷 4181-4184页

作者： Mirko D'Onofrio Giulia Zamboni Alessia Tognolini Roberto malagò niccolò Faccioli Luca Frulloni Roberto Pozzi Mucelli Department of Radiology University of Verona VeronaItaly Department of Gastroenterology University of Verona VeronaItaly

瞄准：在在集体形成的胰腺炎和胰腺的癌之间的鉴别诊断与第二代的造影剂估计提高对比的 ultrasonography (CEUS ) 的用途。方法：从我们的收音机病理数据库，我们检索了经历了 CEUS 的集体形成的胰腺炎或胰腺的癌影响的所有病人。我们... 详细信息

瞄准：在在集体形成的胰腺炎和胰腺的癌之间的鉴别诊断与第二代的造影剂估计提高对比的 ultrasonography (CEUS ) 的用途。方法：从我们的收音机病理数据库，我们检索了经历了 CEUS 的集体形成的胰腺炎或胰腺的癌影响的所有病人。我们由识别“ parenchymographic ”就在集体形成的胰腺炎和胰腺的肿瘤之间的鉴别诊断的可能性而言在 173 胰腺的群众的学习评估了 CEUS 的结果改进在 CEUS 的动态阶段期间，它为集体形成的胰腺炎被认为诊断。结果：在 CEUS， 94% 集体形成的胰腺炎显示出 intralesional parenchymography。CEUS 有 88.6% 的敏感的集体形成的胰腺炎的允许的诊断， 97.8% 的特性， 91.2% 的积极预兆的价值， 97.1% 的否定预兆的价值，和外套 96% 的精确性。CEUS 显著地在在集体形成的胰腺炎和胰腺的癌之间的鉴别诊断增加了诊断信心，与操作从 0.557 的特性曲线区域的接收装置(P = 0.1608 ) 为到 0.956 的基线美国(P < 0.0001 ) 为 CEUS。结论：CEUS 与 96% 的诊断精确性允许集体形成的胰腺炎的诊断。CEUS 显著地在从胰腺的瘤认出集体形成的胰腺炎关于基础美国增加诊断信心。

关键词：胰腺炎超声检查治疗临床

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Expression of Multidrug Resistance ATP-Binding Cassette(ABC)Transporters in Canine Mammary Tumors

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Advances in Breast Cancer Research 2015年第3期4卷 77-85页

作者： Breno S.Salgado Suely nonogaki Luisa M.Soares Angela Akamatsu Cristiano R.n.da Silva Thiago P.Anacleto Rodolfo malagó Rafael M.Rocha Fátima Gartner noeme S.Rocha Departamento de Patologia Faculdade de Medicina de BotucatuUniversidade Estadual PaulistaCampus de BotucatuBotucatuBrazil Instituto de Ciências Biomédicas Abel Salazar Universidade do Porto(Intitute of Biomedical Sciences Abel Salazar of University of Porto)OportoPortugal Fundacao Antonio Prudente Hospital A.C.CamargoSao PauloBrazil Departamento de Clínica Veterinária Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual PaulistaUNESP.BotucatuBrazil Hospital Escola de Medicina Veterinária Fundacao de Ensino e Pesquisa de Itajubá—FEPIItajubáBrazil

Mammary neoplasms are the most common tumors in female dogs. They are usually treated using solely surgical mastectomy—which is recognized as unsatisfactory in many cases. Given this, the benefits of chemotherapy in dogs with mammary cancer need to be further explored. Some drugs that can be used for treating canines with mammary tumors may be substrates of uptake and/or efflux transporters such as the ATP-binding cassette (ABC) transporters. Unfortunately, very little is known regarding the pathobiology of such proteins in canine tumors, including mammary cancer. Accordingly, this study was designed to characterize the expression of ABC transporters P-glycoprotein, MRP1, and MRP2 and their relation with clinicopathologic factors in order to allow a better understanding of their influence in canine mammary cancer. P-glycoprotein was expressed in tumors from 55.8% of patients, while MRP1 and MRP2 were expressed in 37.2% and 39.5% of tumors, respectively. P-glycoprotein expression showed to be related with regional lymph node spread (P = 0.0038), as well as with tumor grade (P = 0.0353) and with a shorter survival (P = 0.0245). MRP1 revealed a strong association with a higher histological grade (P nd overall survival (P = 0.0002). Additionally, MRP1 was determined as prognostic indicator independent of lymph node status using Cox proportional-hazards regression multivariate analysis (P = 0.0216). no relations between MRP2 and clinicopathologic features were observed. We have found that P-glycoprotein and MRP1 are expressed in highly aggressive canine mammary tumors and are related with poor prognosis. Our results suggest that they may play a significant role in the course of canine mammary cancer progression and be promising candidate markers for a validation study on therapy outcome.

关键词： MDR1 P-Gp MRP1 MRP2 ABCC2 Mammary neoplasms Prognosis Dog

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Detection of Babesia bovis and Babesia bigemina in Water Buffaloes (Bubalus bubalis) in Endemic Areas of São Paulo State, Brazil

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Open Journal of Veterinary Medicine 2016年第5期6卷 75-84页

作者： T. A. néo R. Giglioti D. Obregón T. B. Bilhassi H. n. Oliveira R. Z. Machado F. de F. Aníbal L. G. Brito W. malagó Jr. F. A. Bressani M. C. S. Oliveira Department of Morphology and Pathology Universidade Federal de S&atildeo Carlos S&atildeo Carlos Brazil Department of Animal Science Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Jaboticabal Brazil Agrarian University of Havana San José de las Lajas Cuba Department of Veterinary Pathology Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Jaboticabal Brazil Embrapa Rondônia Porto Velho Brazil Embrapa Pecuária Sudeste S&atildeo Carlos Brazil

Babesiosis is a tick-transmitted disease that causes severe economic losses to the cattle industry in Brazil. Water buffaloes (Bubalus bubalis) are often carriers of Babesia spp., but there are no studies that provide an accurate estimation of this infection in animals raised in regions of endemic stability. This study was conducted to investigate Babesia bovis and B. bigemina infections in 108 water buffaloes (50 calves and 58 adult females) located in areas of São Paulo state, where the animals were continuously exposed to Rhipicephalus microplus ticks. B. bovis and B. bigemina infections were screened by microscopic examination of blood smears, nested PCR (nPCR) and quantitative real-time PCR (qPCR), which were also used to estimate the number of copies (nC) of the cytochrome b (mt-cytB) gene in the blood samples. B. bigemina was found in blood smears of three calves from Alambari herd (all with less than 0.1% parasitemia). Molecular techniques were more sensitive than blood smears to diagnose piroplasms in water buffaloes: 20.37% and 100.00% for B. bovis-infected animals and 59.26% and 100.00% for B. bigemina-infected animals, respectively for nPCR and qPCR. The nC of mt-cytB gene of B. bovis and B. bigemina in blood samples revealed significant effects (p p ≤ 0.05) for B. bigemina (2.80 ± 0.06) than for B. bovis (2.61 ± 0.05). Within each herd-age, differences between the species’ nC values were found only in Alambari calves, which showed significantly higher (p ≤ 0.05) nC of B. bigemina (3.48 ± 0.13). The calves and cows from Ibaté showed the lowest nC of B. bigemina (2.29 ± 0.13 and 2.63 ± 0.14) and B. bovis (2.54 ± 0.11 and 2.37 ± 0.12), respectively. These data suggest a high prevalence of B. bovis and B. bigemina infection in the buffalo population in endemic areas of São Paulo state.

关键词： Bubalus bubalis Babesiosis Diagnosis nPCR qPCR

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Risk of venous congestion in live donors of extended right liver graft

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World Journal of Gastroenterology 2015年第19期21卷 6008-6017页

作者： Arnold Radtke George Sgourakis Ernesto P Molmenti Susanne Beckebaum Vito R Cicinnati Hartmut Schmidt Heinz-Otto Peitgen Christoph E Broelsch Massimo malagó Tobias Schroeder Department of General and Transplantation Surgery University Hospital Tuebingen Department of General Surgery of Red Cross Hospital Department of Surgery North Shore University Hospital Manhasset Department of General Visceral and Transplantation SurgeryUniversity Hospital Essen Department of Transplant Medicine University Hospital Muenster MeVis Center for Medical Diagnostic Systems and Visualization University of Bremen Department of Surgery-UCL Division of Surgical and Interventional Sciences University College London Department of Diagnostic and Interventional Radiology University Hospital Essen

AIM: To investigate middle hepatic vein(MHV)management in adult living donor liver transplantation and safer remnant volumes(RV).METHODS: There were 59 grafts with and 12 grafts without MHV(including 4 with MHV-5/8 reconstructions).All donors underwent our five-step protocol evaluation containing a preoperative protocol liver biopsy Congestive vs non-congestive RV, remnantvolumebody-weight ratios(RVBWR) and postoperative outcomes were evaluated in 71 right graft living donors. Dominant vs non-dominant MHV anatomy in total liver volume(d-MHV/TLV vs nd-MHV/TLV) was constellated with large/small congestion volumes(CVindex).Small for size(SFS) and non-SFS remnant considerations were based on standard cut-off- RVBWR and RV/TLV. non-congestive RVBWR was based on non-congestive ***: MHV and non-MHV remnants showed no significant differences in RV, RV/TLV, RVBWR, total bilirubin, or InR. SFS-remnants with RV/TLV nd non-SFS-remnants with RV/TLV ≥ 30% showedno significant differences either. RV and RVBWR for non-MHV(n = 59) and MHV-containing(n = 12)remnants were 550 ± 95 ml and 0.79 ± 0.1 ml vs568 ± 97 ml and 0.79 ± 0.13, respectively(P = 0.423 and P = 0.919. Mean left RV/TLV was 35.8% ± 3.9%.non-MHV(n = 59) and MHV-containing(n = 12)remnants(34.1% ± 3% vs 36% ± 4% respectively,P = 0.148. Eight SFS-remnants with RVBWR nificantly smaller RV/TLV than 63 non-SFSremnants with RVBWR ≥ 0.65 [SFS: RV/TLV 32.4%(range: 28%-35.7%) vs non-SFS: RV/TLV 36.2%(range: 26.1%-45.5%), P nants with RV/TLV nificantly smaller RVBWR than 65 non-SFS-remnants with RV/TLV ≥ 30%(0.65(range: 0.6-0.7) vs 0.8(range: 0.6-1.27), P nors developed reversible liver *** and RV/TLV were concordant in 25%-33%of SFS and in 92%-94% of non-SFS remnants. MHV management options including complete MHV vs MHV-4A selective retention were necessary in n = 12 vs n =2 remnants based on particularly risky congestive and non-congestive

关键词： Living donor liver transplantation Livervolume Remnant volume Small-for-size Small-forsizesyndrome

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Differential IL10 mRnA Profiles Associated to Babesia bovisand B. bigeminaInfection Levels in Persistently Infected Animals

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Open Journal of Veterinary Medicine 2019年第11期9卷 161-169页

作者： Talita Barban Bilhassi Rodrigo Giglioti Cintia Hiromi Okino Wilson malagó Júnior Henrique nunes de Oliveira Cíntia Righetti Marcondes Márcia Cristina de Sena Oliveira Universidade Estadual Paulista Júlio de Mesquita Filho UNESP/FCAV Campus de Jaboticabal Jaboticabal SP Brazil Embrapa Pecuária Sudeste São Carlos SP Brazil Institute of Animal Science (APTA-SAA) Nova Odessa SP Brazil

This work aimed to find quantitative phenotypic traits that can be used to discriminate the levels of resistance/susceptibility to B. bovis and B. bigemina in two groups of cattle presenting the highest (H) or lowest (L) infection levels and Rhipicephalus microplus ticks count. The animals were selected from a previous study of 50 Canchim (5/8 Charolais/zebu) heifers raised in an endemic area for these parasites. These animals were evaluated regarding their TnFα, IL10, IFn-γ, IL12 and inOS mRnA levels. no differences were found between these groups regarding TnFα, IFn-γ, IL12β or inOS transcripts. However, the IL10 transcripts were significantly higher in the H group compared to the L group. Moreover, significant correlation coefficients were observed between B. bovis loads and both IL10 and IFn-γ transcripts, while no correlations were found for B. bigemina loads and all tested immune-related transcripts, suggesting that differential IL10 mRnA profiles were closely associated to B. bovis loads. Our results have contributed to a better understanding of the immune responses against Babesia infection, as we demonstrated that the IL10 cytokine levels might also influence or be influenced by parasitemia levels in persistently infected animals.

关键词： Babesia bovis Cytokines Gene Expression IL10 RT-qPCR

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对冠状动脉CT血管成像的学习曲线:充分训练包括哪些内容?

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国际医学放射学杂志 2009年第4期32卷 378-379页

作者： F.Pugliese M.G.Hunink K.Gruszczynska F.Alberghina R.malagó n.van Pelt 程悦 Department of Radiology Erasmus MC University Medical Centre Rotterdam

目的前瞻性评价实践训练对冠状动脉CT血管成像像上≥50％狭窄段判断能力的影响。方法机构审查委员会批准该研究方案。在传统冠脉造影前进行冠脉CT血管成像的病人被告知将接受额外的放射剂量，所有病人均同意此方案，且同意将他们检查的... 详细信息

目的前瞻性评价实践训练对冠状动脉CT血管成像像上≥50％狭窄段判断能力的影响。方法机构审查委员会批准该研究方案。在传统冠脉造影前进行冠脉CT血管成像的病人被告知将接受额外的放射剂量，所有病人均同意此方案，且同意将他们检查的资料用于后续研究。选择无冠脉CT血管成像经验的3名放射科医生和1名心脏科医生接受为期1年的心脏CT培训。学员每周完成12．15例冠脉CT的采集和影像读片（1年约为600例）。为评价其诊断能力的提高，

关键词： CT血管成像冠状动脉学习曲线训练放射科医生冠脉造影前瞻性评价判断能力

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