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Response of Explants of Calli Rice (<i>Oryza sativa</i>L.) Japonica cv. “Ilmi” to Gene Transformation Using <i>Agrobacterium tumefaciens</i>-Mediated

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American Journal of Plant Sciences 2013年第4期4卷 838-843页

作者： fika ayu safitri Mohammad Ubaidillah Miswar Kyung-Min Kim Agrotechnology Study Program Faculty of Agriculture Jember University Jember Indonesia Division of Plant Biosciences School of Applied Biosciences College of Agriculture & Life Science Kyungpook National University Daegu South Korea

The AtBI-1 gene encoding the Arabidopsis thaliana Bax inhibitor was introduced into Japonica cultivars of rice (“Ilmi”) by Agrobacterium-mediated transformation, and a large number of transgenic plants were produced. The neomycin phosphotransferase II (NPTII) gene was used as a selectable marker. The activity of neomycin phosphotransferase could be successfully detected in transgenic rice calluses. Introduction of the AtBI-1 gene was also confirmed by PCR using AtBI-1 specific oligonucleotide primers in regenerated plants. Stable integration and expression of the AtbI-1 gene in plants were confirmed by GFP analysis.

关键词： AtBI-1 Agrobacterium-Mediated GFP Transformation Rice

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Between food fulfillment and income:Can urban agriculture contribute to both?

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Geography and Sustainability 2023年第2期4卷 127-137页

作者： Oekan S.Abdoellah Yusep Suparman Kinanti Indah safitri Akhmad Zainal Mubarak Mira Milani Margareth Levaldo Surya Department of Anthropology Faculty of Social and Political SciencesUniversitas PadjadjaranSumedang 45363Indonesia Center for Environment and Sustainability Science Universitas PadjadjaranBandung 40134Indonesia Department of Statistics Faculty of Mathematics and Natural ScienceUniversitas PadjadjaranSumedang 45363Indonesia Graduate studies on Environmental Sciences Universitas PadjadjaranBandung 40115Indonesia Politechnic Bandung Health Nutrition Department Bandung 40559Indonesia

Food and Agriculture Organization(FAO)is appealing for a global implementation of urban agricultural strate-gies to increase food availability.Bandung,a city in Indonesia,is already participating in this initiative.This research aimed to assess the contribution of urban agriculture toward household food fulfillment and income.Furthermore,the impacts on urban,subsistence,semi-commercial,and commercial agriculture were considered.In-depth interviews to obtain qualitative data and observations were also utilized.A survey of 321 households,consisting of 107 for each urban agricultural type,used questionnaires to obtain quantitative data.These inputs were further analyzed to determine the contribution of household food fulfillment and income.The results showed that average urban agriculture has an insignificant and significant contribution of 9%and 41.5%to household food fulfillment and income.This was due to the vast complexities of metropolitan problems,including limited access to land,lack of participation,dependence on on-market products,and increasing commercialization which opposed urban agricultural policies。

关键词： Urban agriculture Food fulfillment Income

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Self Regulation and Learning Environment:Online Learning Survey of High School Students in Indonesia

Self Regulation and Learning Environment:Online Learning Sur...

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作者： Nur Shabrina safitri 华中师范大学

学位级别：硕士

本研究旨在了解高中生的自我调节与建构主义在线学习环境对学习成绩的相关性。印尼主义教育运动不仅是为了发展知识,更是为了实现高尚的态度。学生的高尚态度可从学习过程中得到认可,在线学习过程也不例外。然而,在线学习过程中,学生往... 详细信息

本研究旨在了解高中生的自我调节与建构主义在线学习环境对学习成绩的相关性。印尼主义教育运动不仅是为了发展知识,更是为了实现高尚的态度。学生的高尚态度可从学习过程中得到认可,在线学习过程也不例外。然而,在线学习过程中,学生往往会因为被其他媒体分散注意力而失去注意力。此外,在线学习过程中,教师无法充分监督学生学习。学生需要自我调节以达到预期的学习目标。学习环境也与学习结果有关。在线学习环境的重要性与线下学习环境同样重要。于印尼莫约克托2021/2022年奇数学期该进行研究对于103名高中生的。本研究为定量研究用相关性检验和F检验。数据的收集使用了一种与之前的研究相适应的工具,同时仍在进行测试,以确定该工具对研究对象的有效性和可靠性。使用研究适应研究收集数据。本研究的结论是自我调节与学习结果之间有相关(r=0.386)与学习环境跟学习結果之间有相关(r=0.410).该值表明,学生自我调节与学习结果之间存在显著的正相关关系,在线学习环境与学习结果之间存在显著的正相关关系。自我调节与在线学习环境也与学生的学习結果有相关(F=0.001).于将来的研究,建议在课堂上使用直接评估与观察所以结果可能会更持续。

关键词：自我调节在线学习环境学习结果高中生

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East Java Propolis Inhibits Cytokine Pro-inflammatory in Odontoblast-Like Cells Human Pulp

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Journal of Agricultural Science and Technology(A) 2014年第1期4卷 27-32页

作者： Ira Widjiastuti Tamara Yuanita James Hutagalung Mandojo Rukmo Indri safitri Department of Conservative Dentistry Faculty of Dentistry Airlangga University Surabaya 60132 Indonesia Institute of Tropical Deases Airlangga University Surabaya 60132 Indonesia Department of Biochemistry Faculty of Medicine Airlangga University Surabaya 60132 Indonesia

Inflammation in pulp tissue is caused by caries bacteria. Most bacteria found are Lactobacillus acidophilus. Propolis is a sticky resin material that is derived from the bees and the surrounding plants, which are reported to have several biological effects including anti-bacterial and anti-inflammatory. This study will prove the effectiveness of propolis against proinflammatory cytokines on odontoblast-like cells in human dental pulp. This study was performed on cultured odontoblast-like cells in pulp. Cell culture was derived from the pulp tissue of human M3 teeth that had been extracted. Odontoblast pulp culture was divided in three groups: group one, cultured cells with propolis; group two, cultured cells induced with inactive Lactobacillus acidophilus; group three, cultured cells induced with inactive Lactobacillus acidophilus and exposed to 3 g/mL propolis. A measurement of TNF- and TGF- 1 expression was done using the immunocytochemical technique to determine the effectiveness of propolis extracts from East Java Indonesia against proinfalmmatory cytokines. Data were analyzed using the analysis of variance (ANOVA) test (P = 0.05). Propolis extract can inhibit the expression of TNF- and increase the expression of TGF- 1 on odontoblast-like cell in human dental pulp. Anti-inflammatory effects of East Java propolis extract are associated with cytokine modulation.

关键词：成牙本质细胞细胞因子蜂胶液牙髓爪哇蜂胶提取物嗜酸乳杆菌 TNF-α

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