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Nano Research 2015年第11期8卷 3704-3714页

作者： Lourdes Rivas Alfredo de la Escosura-Muniz Lorena Serrano laura Altet Olga Francino ArmandSanchez Arben Merkoci ICN2-Nanobioelectronics & Biosensors Group Institut Catala de Nanociencia i Nanotecnologia Campus UAB 08193 Bellaterra (Barcelona) Spain Autonomous University of Barcelona Campus UAB 08193 Bellaterra (Barcelona) Spain Vetgenomics Edifici Eureka Parc de Recerca UAB 08193 Bellaterra (Barcelona) Spain Centre for Research in Agricultural Genomics-CSIC-IRTA-UAB-UB Campus UAB 08193 Cerdanyola del Varies Catalonia Spain ICREA - Institucio Catalana de Recerca i Estudis A vancats 08010 Barcelona Spain

A novel triple lines lateral-flow assay （LFA） with enhanced sensitivity for the detection of Leishmania infantum DNA in dog blood samples was designed and successfully applied. The enhanced LFA methodology takes advantage of the gold nanoparticle tags （AuNPs） conjugated to polyclonal secondary antibodies, which recognize anti-FITC antibodies. The polyclonal nature of the secondary antibodies allows for multiple binding to primary antibodies, leading to enhanced AuNP plasmonics signal. Furthermore, endogenous control consisting of the amplified dog 18S rRNA gene was introduced to avoid false negatives. Using this strategy, 0.038 spiked Leishmania parasites per DNA amplification reaction （1 parasite/100 μL of DNA sample） were detected. detection limit of LFA was found to be lower than that of the conventional techniques. In summary, our novel LFA design is a universal and simple sensing altemative that can be extended to several other biosensing scenarios.

关键词： lateral-flow assay gold nanoparficles secondary antibodies Leishmania DNA endogenous control

The management of the long head of the biceps in rotator cuff repair:A comparative study of high *** tenodesis

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Journal of Sport and Health Science 2023年第5期12卷 613-618页

作者： Edoardo Franceschetti Edoardo Giovannetti de Sanctis Alessio Palumbo Michele Paciotti Luca la Verde Nicola Maffulli Francesco Franceschi department of Orthopaedic and Trauma Surgery Campus Biomedico University of RomeVia Alvaro del Portillo 20000100 RomeItaly department of Orthopaedics and Traumatology Catholic UniversityAgostino Gemelli Hospital00100 RomeItaly Anca Surgical Center Via Francesco Maidalchini 20Roma00152RomeItaly department of Musculoskeletal Disorders Via Salvador Allende4384081 BaronissiSalernoItaly Centre for Sports and Exercise Medicine Barts and The London School of Medicine and DentistryMile End Hospital275 Bancroft RoadLondon E14DGEnglandUK School of Pharmacy and Bioengineering Keele University School of MedicineThornburrow DriveStoke on Trent ST4EnglandUK

Background:Tenodesis of the long head of the biceps(LHB)is commonly undertaken during arthroscopic rotator cuff *** assessed the clinical and structural outcomes after high arthroscopic tenodesis(HAT)or mini-open subpectoral tenodesis(ST).We hypothesized that the clinical and structural results after HAT and ST are ***:We included 40 patients with rotator cuff tear and LHB *** patients(7 women and 13 men;mean age:57.9 years;range:56-63 years)were treated using HAT,and 20 patients(8 women and 12 men;mean age:58.5 years;range:55-64 years)were treated using *** evaluation was performed preoperatively and at 6 weeks,6 months,and 1 year after surgery,using the Constant Murley Score and Simple Shoulder Test scores;the LHB was evaluated using the LHB score.A Visual Analogue Scale was administered to all patients preoperatively and 2 days after ***:The postoperative total and pain subscale’s Constant scores were significantly higher in the ST ***,2 LHB score values were significantly different between the *** postoperative LHB total score in the ST and HAT groups averaged 86.9±4.1(mean±SD)points and 73.3±6.4 points,*** Pain/Cramps subscale in the ST and HAT groups averaged 47.1±5.9 and 33.2±4.6 points,*** 2 groups showed no difference in Visual Analogue Scale values(5.5 in the HAT group;5.8 in the ST group)*** patient in the HAT group reported a secondary onset of Popeye ***:Both high arthroscopic and mini-open ST of the LHB tendon produced reliably good functional results,but the ST group was associated with better postoperative clinical outcomes.

关键词： Long head of the biceps Shoulder Shoulder arthroscopy Subpectoral TenodesisTagedend

Strain-induced spatially indirect exciton recombination in zinc-blende/wurtzite CdS heterostructures

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Nano Research 2015年第9期8卷 3035-3044页

作者： dehui Li Yang Liu Maria de la Mata Cesar Magen Jordi Arbiol Yuanping Feng Qihua Xiong Division of Physics and Applied Physics School of Physical and Mathematical Sciences Nanyang Technological University Singapore 637371 Singapore Institut de Ciencia de Materials de Barcelona ICMAB-CSIC Campus de la UAB 08193 Bellaterra Catalonia Spain laboratofio de Microscopias Avanzadas (LMA) Instituto de Nanociencia de Aragon (INA) ARAID and departamento de Fisica de la Materia Condensada Universidad de Zaragoza 50018 Zaragoza Spain Institucio Catalana de Recerca i Estudis Avancats (ICREA) 08010 Barcelona Catalonia Spain Institut Catala de Nanociencia i Nanotecnologia (ICN2) Campus UAB 08193 Bellaterra Catalonia Spain department of Physics National University of Singapore 2 Science Drive 3 Singapore 117542 Singapore Division of Microelectronics School of Electrical and Electronic Engineering Nanyang Technological University Singapore 639798Singapore

Strain engineering provides an effective mean of tuning the fundamental properties of semiconductors for electric and optoelectronic applications. Here we report on how the applied strain changes the emission properties of hetero- structures consisting of different crystalline phases in the same CdS nanobelts. The strained portion was found to produce an additional emission peak on the low-energy side that was blueshifted with increasing strain. Furthermore, the additional emission peak obeyed the Varshni equation with temperature and exhibited the band-filling effect at high excitation power. This new emission peak may be attributed to spatially indirect exciton recombination between different crystalline phases of CdS. First-principles calculations were performed based on the spatially indirect exciton recombination, and the calculated and experimental results agreed with one another. Strain proved to be capable of enhancing the anti-Stokes emission, suggesting that the efficiency of laser cooling may be improved by strain engineering.

关键词： strain,CdS nanobelts,photoluminescence,spatially indirect excitonrecombination,inter-crystalline phasetransition

Mapping summer soybean and corn with remote sensing on Google Earth Engine cloud computing in Parana state – Brazil

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International Journal of Digital Earth 2020年第12期13卷 1624-1636页

作者： Alex Paludo Willyan Ronaldo Becker Jonathan Richetti laíza Cavalcante de Albuquerque Silva Jerry Adriani Johann Agricultural Engineering(PGEAGRI) Western ParanáState University(UNIOESTE)CascavelBrazil Geotechnology and Data Science Research Center(GeoScience) Western ParanáState University(UNIOESTE)CascavelBrazil

Brazilian farming influences directly the worldwide ***,fast and reliable information on areas sown with the main crops is essential for planning logistics and public or private commodity market *** farming practices have embraced remote sensing to provide fast and reliable information on commodity ***-to-low resolution free orbital images,such as those from landsat 8 and Sentinel 2,have been used for crop mapping;however,satellite image processing requires high computing power,especially when monitoring vast ***,cloud data processing has been the only feasible option to deal with a large amount of orbital data and its processing and ***,our goal was to develop a method to map the two main crops(soybeans and corn)in Paraná,one of the major Brazilian state ***-8,Sentinel-2,SRTM+,and field data from 2016 to 2018 were used with the Simple Non-Iterative Clustering segmentation method and the Continuous Naive Bayes classifier,to identify cropped areas.A minimum global accuracy of 90%was found for both *** with field data showed correlations of 0.96 and agreement coefficients no lower than *** ensures mapping quality when using Sentinel and/or landsat imagery on the GEE platform.

关键词： Continuous Naive Bayes segmentation machine learning land classification

Biochar insights from laboratory incubations monitoring O_(2) consumption and CO_(2) production

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Biochar 2019年第3期1卷 249-258页

作者： Risely Ferraz Almeida Kurt A.Spokas Daniel de Bortoli Teixeira Newton la Scala Junior University of Sao Paulo-USP PiracicabaBrazil United States department of Agriculture-Agricultural Research Service St.PaulMNUSA University of Marília-Unimar MaríliaBrazil Universidade Estadual Paulista-UNESP JaboticabalBrazil

Biochar has been touted as a long-term carbon sequestration ***,there are no studies evaluating biochar’s effect on oxygen(O_(2))consumption as a measure of the microbial respiration response to *** gain insight into this aspect,we evaluated O_(2) consumption rates to test the hypothesis that biochar is an efficient agent for carbon dioxide(CO_(2))sequestration in *** different biochar types and one activated charcoal were incubated alone and associated with three different soils for approximately 2 months in laboratory *** concentration of CO_(2) and O_(2) was periodically *** data presented here confirm that the CO_(2) production following biochar’s addition to soils results in a process that is correlated to oxygen ***,this overall stimulation is not clearly related to biochar *** carbon resulted in the highest statistically significant stimulation of activity,despite it possessing the lowest quantity of volatile carbon and mineral nutrient *** into consideration our results,we conclude that using biochar does achieve total carbon ***,the amount of available soil organic carbon following soil incorporation appears to be reduced following biochar addition and its long-term implication on this mineralizable soil organic carbon pool does deserve more research attention.

关键词： Biochar activation Charcoal CO_(2)sequestration CO_(2)sorption

Adipokines,cytokines and body fat stores in hepatitis Cvirus liver steatosis

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World Journal of Hepatology 2016年第1期8卷 74-82页

作者： emilio gonzález-reimers javier lópez-prieto geraldine quintero-platt ricardo pelazas-gonzález m remedios alemán-valls onán pérez-hernández m joséde-la-vega-prieto m angeles gómez-rodríguez candelaria martín-gonzález francisco santolaria-fernández Servicio de Medicina Interna Hospital Universitario de CanariasUniversidad de La Laguna38320 Canary IslandsSpain

AIM: To identify patients with or without liver steatosis and its severity in treatment-na?ve patients affected by hepatitis C virus(HCV) ***: We included 56 HCV infected patients, and assessed the amount of liver fat by histomorphometry, and its relationships with fat and lean mass at different parts of the body(by densitometry), hormones [insulin, homeostatic model assessment(HOMA)], adipokines(resistin, adiponectin, leptin), and cytokines(tumor necrosis factor α, interleukin-6).RESULTS: Although the intensity of liver steatosis is related to trunk fat mass and HOMA, 33% of patients showed no liver steatosis, and this finding was not related to body mass index or genotype. Besides trunkfat mass, no other factor was related to the presence or not of liver steatosis, or to the intensity of it, by multivariate analysis. Lean mass was not related to liver steatosis. Adiponectin levels were lower among patients. No differences were observed in leptin and ***: Steatosis in HCV infection is common(67.2%), and closely related to trunk fat, and insulin resistance, but not with leg fat mass or adipokines.

关键词： Resistin Adiponectin Insulin resistance Proinflammatory cytokines Leptin Hepatitis C virus Liver steatosis

Exploring alternative treatments for Helicobacter pylori infection

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World Journal of Gastroenterology 2014年第6期20卷 1450-1469页

作者： Guadalupe Ayala Wendy Itzel Escobedo-Hinojosa Carlos Felipe de la Cruz-Herrera Irma Romero Centro de Investigación sobre Enfermedades Infecciosas Instituto Nacional de Salud Pública INSP departamen-to de Bioquímica Facultad de Medicina Universidad Nacional Autónoma de México

Helicobacter pylori(H. pylori) is a successful pathogen that can persist in the stomach of an infected person for their entire life. It provokes chronic gastric inflammation that leads to the development of serious gastric diseases such as peptic ulcers, gastric cancer and Mucosa associated lymphoid tissue lymphoma. It is known that these ailments can be avoided if the infection by the bacteria can be prevented or eradicated. Currently, numerous antibiotic-based therapies are available. However, these therapies have several inherent problems, including the appearance of resistance to the antibiotics used and associated adverse effects, the risk of re-infection and the high cost of antibiotic therapy. The delay in developing a vaccine to prevent or eradicate the infection has furthered research into new therapeutic approaches. This review summarises the most relevant recent studies on vaccine development and new treatments using natural resources such as plants, probiotics and nutraceuticals. In addition, novel alternatives based on microorganisms, peptides, polysaccharides, and intragastric violet light irradiation are presented. Alternative therapies have not been effective in eradicating the bacteria but have been shown to maintain low bacterial levels. Nevertheless, some of them are useful in preventing the adverse effects of antibiotics, modulating the immune response, gastroprotection, and the general promotion of health. Therefore, those agents can be used as adjuvants of allopathic anti-H. pylori eradication therapy.

关键词： Helicobacter pylori Treatment Natural products Probiotics Nutraceuticals

The sound of host-SARS-CoV-2 molecular interactions

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The Innovation 2021年第3期2卷 5-6页

作者： Joséde la Fuente Juan J.Pastor Comín Christian Gortázar SaBio.Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM Ronda de Toledo s/n13005 Ciudad RealSpain department of Veterinary Pathobiology Center for Veterinary Health SciencesOklahoma State UniversityStillwaterOK 74078USA Centro de Investigación y Documentación Musical CIDoM-UCLM-CSIC Facultad de Educación de Ciudad RealRonda Calatrava 313071 Ciudad RealSpain

Host-and virus-derived factors are key drivers of the COVID-19 pandemic(Gortázar et al.).1 The interaction between the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)spike glycoprotein(S)and host cell membrane angiotensin I converting enzyme 2(ACE2)receptor is highly dynamic,mediates virus infection,and is the main target for protective antibody response2.

关键词： ACE2 protective acute

Physical exercise in the prevention and treatment of Alzheimer’s disease

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Journal of Sport and Health Science 2020年第5期9卷 394-404页

作者： Adrian de la Rosa Gloria Olaso-Gonzalez Coralie Arc-Chagnaud fernando Millan Andrea Salvador-Pascual Consolacion Garcıa-Lucerga Cristina Blasco-lafarga Esther Garcia-Dominguez Aitor Carretero Angela G.Correas Jose Vina Mari Carmen Gomez-Cabrera Freshage Research Group Department of PhysiologyFaculty of MedicineUniversity of Valenciaand CIBERFESInsitute of Health Research-INCLIVAValencia 46010Spain INRA UMR866 Muscle dynamics and metabolismUniversity of MontpellierF-34060MontpellierFrance department of Physiotherapy Faculty of PhysiotherapyUniversity of ValenciaValencia 46010Spain Physical Education and Sports department University of ValenciaValencia 46010Spain

dementia is one of the greatest global challenges for health and social care in the 21st ***’s disease(AD),the most common type of dementia,is by no means an inevitable consequence of growing *** lifestyle factors may increase,or reduce,an individual’s risk of developing *** has been written over the ages about the benefits of exercise and physical *** the risk factors associated with AD is a low level of physical *** relationship between physical and mental health was established several years *** this review,we discuss the role of exercise(aerobic and resistance)training as a therapeutic strategy for the treatment and prevention of *** adults who exercise are more likely to maintain *** address the main protective mechanism on brain function modulated by physical exercise by examining both human and animal *** will pay especial attention to the potential role of exercise in the modulation of amyloid b turnover,inflammation,synthesis and release of neurotrophins,and improvements in cerebral blood *** changes in lifestyle in presymptomatic and predementia disease stages may have the potential for delaying one-third of dementias *** interventions that include the adoption of an active lifestyle should be recommended for older populations.

关键词： Aerobic exercise dementia Exercise training Lifestyle factors Multidomain interventions Resistance exercise

Creeping fat and gut microbiota in Crohn’s disease

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World Journal of Gastroenterology 2025年第1期31卷 16-23页

作者： Ana EV Quaglio Daniéla O Magro Marcello Imbrizi Ellen CS de Oliveira Luiz C Di Stasi Ligia Y Sassaki Verum Ingredients Botucatu Technology ParkBotucatu 18605-525São PauloBrazil department of Surgery Faculty of Medical SciencesState University of CampinasCampinas 13083-970São PauloBrazil department of Gastroenterology Faculty of Medical SciencesUniversity of CampinasCampinas 13083-970São PauloBrazil department of Internal Medicine Medical SchoolSão Paulo State UniversityBotucatu 18618-686São PauloBrazil department of Biophysics and Pharmacology Institute of BiosciencesSão Paulo State UniversityBotucatu 18618-689São PauloBrazil

In this article,we explored the role of adipose tissue,especially mesenteric adipose tissue and creeping fat,and its association with the gut microbiota in the pathophysiology and progression of Crohn’s disease(CD).CD is a form of inflammatory bowel disease characterized by chronic inflammation of the gastrointestinal tract,influenced by genetic predisposition,gut microbiota dysbiosis,and environmental *** microbiota plays a crucial role in modulating immune response and intestinal inflammation and is associated with the onset and progression of ***,visceral adipose tissue,particularly creeping fat,a mesenteric adipose tissue characterized by hypertrophy and fibrosis,has been implicated in CD pathogenesis,inflammation,and *** bacteria from the gut microbiota may translocate into mesenteric adipose tissue,contributing to the formation of creeping fat and influencing CD *** creeping fat may be a protective barrier against bacterial invasion,its expansion can damage adjacent tissues,leading to *** gut microbiota through interventions such as fecal microbiota transplantation,probiotics,and prebiotics has shown potential in managing ***,more research is needed to clarify the mechanisms linking gut dysbiosis,creeping fat,and CD progression and develop targeted therapies for microbiota modulation and fat-related complications in patients with CD.

关键词： Creeping fat Mesenteric adipose tissue Gut microbiota Crohn’s disease Inflammatory bowel disease