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Distributed Differences Structures Underlie Gating between the Kin Channel KAT1 and the Kout Channel SKOR

Molecular Plant 2010年第1期3卷 236-245页

作者： Janin Riedelsberger Tripti Sharma Wendy gonzalez Pawel Gajdanowicz Samuel Elias Morales-Navarro Carlos Garcia-Mata Bernd Mueller-Roeber fernando danilo gonzalez-nilo Michael R. Blatt Ingo Dreyer Universitat Potsdam Institut for Biochemie und Biologie Molekularbiologie Heisenberg-Gruppe Biophysik und Molekulare Pflanzenbiologie BPMPB Karl-Liebknecht-Strasse 24-25 Haus 20 D-14476 Potsdam-Golm Germany Max-Planck Institute of Molecular Plant Physiology 14476 Potsdam-Golm Germany Centro de Bioinformatica y Simulacion Molecular Universidad de Talca Casilla 721 Talca Chile Laboratory of Plant Physiology and Biophysics IBLS Plant Sciences Bower Building University of Glasgow Glasgow G12 8QQ UK Laboratorio de Fisiologia Molecular e Integrativa Institutos de Investigaciones Biologicas Universidad Nacional de Mar del Plata 7600 Mar del Plata Buenos Aires Argentina Universitat Potsdam Institut for Biochemie und Biologie Abteilung Molekularbiologie 14476 Potsdam-Golm Germany

The family of voltage-gated （Shaker-like） potassium channels in plants includes both inward-rectifying （Kin） channels that allow plant cells to accumulate K＋ and outward-rectifying （Kout） channels that mediate K＋ efflux. Despite their dose structural similarities, Kin and Kout channels differ in their gating sensitivity towards voltage and the extracellular K＋ concentration. We have carried out a systematic program of domain swapping between the Kout channel SKOR and the Kin channel KAT1 to examine the impacts on gating of the pore regions, the S4, S5, and the S6 helices. We found that, in particular, the N-terminal part of the S5 played a critical role in KAT1 and SKOR gating. Our findings were supported by molecular dynamics of KAT1 and SKOR homology models. In silico analysis revealed that during channel opening and closing, displacement of certain residues, especially in the S5 and S6 segments, is more pronounced in KAT1 than in SKOR. From our analysis of the S4-S6 region, we conclude that gating （and K＋-sensing in SKOR） depend on a number of structural elements that are dispersed over this -145-residue sequence and that these place additional constraints on configurational rearrangement of the channels during gating.

关键词： Arabidopsis K+ channel outward rectifier inward rectifier channel protein structure channel protein-cation interaction gating, K+-dependent.

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A mutation in the ZNF687 gene that is responsible for the severe form of Paget's disease of bone causes severely altered bone remodeling and promotes hepatocellular carcinoma onset in a knock-in mouse model

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Bone Research 2023年第1期11卷 201-214页

作者： Sharon Russo Federica Scotto di Carlo Antonio Maurizi Giorgio Fortunato Anna Teti danilo Licastro Carmine Settembre Tommaso Mello fernando Gianfrancesco Institute of Genetics and Biophysics“Adriano Buzzati-Traverso” National Research Council of ItalyNaplesItaly Department of Environmental Biological and Pharmaceutical Sciences and Technologies(DiSTABiF)University of Campania Luigi VanvitelliCasertaItaly Department of Biotechnological and Applied Clinical Sciences University of L’AquilaL’AquilaItaly AREA Science Park PadricianoTriesteItaly Telethon Institute of Genetics and Medicine(TIGEM) PozzuoliItaly Gastroenterology Unit Department of Experimental and Clinical Biochemical Sciences“Mario Serio”University of FlorenceFlorenceItaly IRCCS INM Neuromed PozzilliISItaly

Paget’s disease(PDB)is a late-onset bone remodeling disorder with a broad spectrum of symptoms and *** of the most aggressive forms is caused by the P937R mutation in the ZNF687 *** the genetic involvement of ZNF687 in PDB has been extensively studied,the molecular mechanisms underlying this association remain ***,we describe the first Zfp687 knock-in mouse model and demonstrate that the mutation recapitulates the PDB phenotype,resulting in severely altered bone *** microcomputed tomography analysis,we observed that 8-month-old mutant mice showed a mainly osteolytic phase,with a significant decrease in the trabecular bone volume affecting the femurs and the ***,osteoblast activity was deregulated,producing disorganized ***,this phenotype became pervasive in 16-month-old mice,where osteoblast function overtook bone resorption,as highlighted by the presence of woven bone in histological analyses,consistent with the PDB ***,we detected osteophytes and intervertebral disc degeneration,outlining for the first time the link between osteoarthritis and PDB in a PDB mouse *** sequencing of wild-type and Zfp687 knockout RAW264.7 cells identified a set of genes involved in osteoclastogenesis potentially regulated by Zfp687,e.g.,Tspan7,Cpe,Vegfc,and Ggt1,confirming its role in this ***,in this mouse model,the mutation was also associated with a high penetrance of hepatocellular ***,this study established an essential role of Zfp687 in the regulation of bone remodeling,offering the potential to therapeutically treat PDB,and underlines the oncogenic potential of ZNF687.

关键词： RAW264.7 Paget remodeling

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Separation of middle rare earths by solvent extraction using 2-ethylhexylphosphonic acid mono-2-ethylhexyl ester as an extractant

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Journal of Rare Earths 2009年第5期27卷 830-833页

作者： danilo Fontana Loris Pietrelli ENEA Italian National Agency for New TechnologiesEnergy and the Environment Via Anguillarese 301I-00123 RomaItaly

The extraction of the trivalent middle rare earths from chloride media by kerosene solutions of 2-ethylhexylphosphonic acid mono-2-ethylhexyl ester as an extractant was studied. The separation factors between the elements using solution simulating wastes from NiMH spent batteries have been evaluated: the order of the extractive ability of extractant can be confirmed in Tb>Gd>Eu>Sm.

关键词： solvent extraction rare earths separation

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Some stability results for a model of Hepatitis C including alanine aminotransferase and immune system

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International Journal of Biomathematics 2020年第8期13卷 157-185页

作者： Salvo danilo Lombardo Sebastiano Lombardo CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences Lazarettgasse 14AKH BT 25.3 A-1090 ViennaAustria Department of Mathematics and Computer Science University of Catania(Ret.) 95125CataniaItaly

In clinical practice,many cirrhosis scores based on alanine aminotransferase(ALT)levels *** the most recent direct acting antivirals(DAAs)reduce fibrosis and ALT levels,the Hepatitis C virus(HCV)is not always *** this paper,we study a mathematical model of the HCV virus,which takes into account the role of the immune system,to investigate the ALT behavior during *** find five equilibrium points and analyze their stability.A sufficient condition for global asymptotical stability of the infeetion-free equilibrium is obtained and local asymptotical stability conditions are given for the immune-free infection and cytotoxic T lymphocytes(CTL)response *** stability of the infection equilibrium with the full immune response is numerically performed.

关键词： Hepatitis C virus(HCV) direct antiviral agents(DAAs) ordinary differential equations(ODEs) equilibria stability basic reproduction number

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Comparative analysis of permissioned blockchain frameworks for industrial applications

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Blockchain(Research and Applications) 2023年第1期4卷 58-70页

作者： Vittorio Capocasale danilo Gotta Guido Perboli Department of Control and Computer Engineering Politecnico di Torino10129 TurinItaly Service Innovation TIMTurinItaly Department of Management and Production Engineering Politecnico di Torino10129 TurinItaly

Blockchain is a technology that creates trust among non-trusting parties without relying on any ***,it has attracted the interest of companies operating in a multitude of ***,due to the number of different blockchain solutions that have emerged in the last few years and their rapid changes,it is challenging for such companies to orient their technological *** paper presents a comparative analysis of the key dimensions—namely,governance,maturity,support,latency,privacy,interoperability,flexibility,efficiency,resiliency,and scalability—of some of the most-used permissioned blockchain ***,we present the results of a performance evaluation considering the following frameworks:Hyperledger Fabric 2.2,Hyperledger Sawtooth 1.2,and ConsenSys Quorum 21.1(with both the GoQuorum client and the Hyperledger Besu client).The platforms were tested under similar conditions,and official releases were used,such that our findings provide a reference for companies establishing their technological orientation.

关键词： Blockchain performance evaluation Hyperledger Besu Hyperledger Fabric Hyperledger Sawtooth Quorum

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Aerodynamic optimization of an adaptive flap for next-generation green aircraft

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Chinese Journal of Aeronautics 2024年第2期37卷 100-122页

作者： Tianlong LIN Rosario PECORA danilo CILIBERTI Wei XIA Shuling HU State Key Laboratory for Strength and Vibration of Mechanical Structures School of Aerospace EngineeringXi’an Jiaotong UniversityXi’an 710049China Deparment of Industrial Engineering Aerospace DivisionUniversity of Naples"FEDERICO II"Napoli 80125Italy

Adaptive,morphing flaps are taking ever-increasing attention in civil aviation thanks to the expected benefits this technology can bring at the aircraft level in terms of high-lift performance improvement and related fuel burnt reduction per *** upon morphing capabilities,it is possible to fix a unique setting for the flap and adapt the flap shape to match the aerodynamic requirements for take-off or *** proper morphed shapes can assure better high-lift performances than those achievable by referring to a conventional ***,standing the unique flap setting for take-off and landing,a dramatic simplification of the flap deployment systems may be *** a consequence of this simplification,the deployment system can be fully hosted in the wing,thus avoiding under-wing nacelles with significantly better aerodynamics and fuel *** first step for a rational design of an adaptive flap consists in defining the target morphed shapes and the unique optimal flap setting in the take-off and landing *** this work,aerodynamic optimization analyses are carried out to determine the best flap setting and related morphed shapes in compliance with the take-off and landing requirements of a reference civil transport *** different initial conditions are adopted to avoid the optimization falling into local optima,thus obtaining four groups of optimal candidate *** comparing each candidate’s performance through 2D and 3D simulations,the optimal configuration has been selected.2D simulations show that the optimal configuration is characterized by a maximum lift increase of 31.92%in take-off and 9.04%in *** to 3D simulations,the rise in maximum lift equals 22.26%in take-off and 3.50%in *** results are finally verified through wind tunnel tests,and the aerodynamic mechanism behind the obtained improvements is explained by carefully analyzing the flow field around the flap.

关键词： Morphing wings High-lift system Adaptive flap Computational Fluid Dynamics(CFD) Wind tunnel tests

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Economic and Qualitative Traits of Italian Alps Saffron

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Journal of Mountain Science 2015年第6期12卷 1542-1550页

作者： Alessandra MANZO Sara PANSERI danilo BERTONI Annamaria GIORGI Centre for Applied Studies in the Sustainable Management and Protection of the Mountain Environment-Ge.S.Di.Mont.-Università degli Studi di Milano Department of Veterinary Science and Public Health-Università degli Studi di Milano Department of Economics Management and Quantitative Methods-Università degli Studi di Milano Department of Agricultural and Environmental Sciences-Production Landscape Agroenergy-Università degli Studi di Milano

Saffron, obtained from the flower stigmas of Crocus sativus L., is one of the most expensive food spices. The introduction of saffron in alpine areas could help to broaden and diversify the activities of mountain multifunctional farms, with a positive impact on economy and land management. According to ISO 3632(2010/2011), saffron can be classified into three categories of quality(I, II, III) depending on the concentration of the three main metabolites responsible for its characteristic colour, flavor and aroma: Crocin, Picrocrocin and Safranal. This study represents the first investigation of the quality of saffron produced in the Italian Alps evaluated with spectrophotometry, HPLC, solid-phase microextraction(SPME), and gas chromatographic analysis combined with mass spectrometry(GC/MS). The experiments used Crocus sativus stigmas produced in 2012-2013 in different areas of the Central Italian Alps were located at an altitude between 720 and 1200 m a.s.l.. Results obtained were compared to commercial saffron. The analyses confirmed that all samples can be classified in the first quality category according to the ISO classification. This high quality is also confirmed by HPLC analysis. Moreover, the SPME-GC/MS analysis identified some differences in the aromatic profile of saffron samples, in particular regarding safranal concentration. A preliminary assessment of the economic viability of high quality saffron production for local markets was also performed. Our study provides valid information regarding the quality and economic sustainability of saffron production in the alpine area confirming this crop as a good candidate for a new source of income for multifunctional farms in mountain areas.

关键词： Crocus sativus L. Alps ISO 3632 UV– Vis spectrophotometry SPME-GC/MS HPLC

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Design of hybrid-electric aircraft with fault-tolerance considerations

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Chinese Journal of Aeronautics 2023年第2期36卷 160-178页

作者： Valerio MARCIELLO Francesco OREFICE Fabrizio NICOLOSI danilo CILIBERTI Pierluigi DELLA VECCHIA Department of Industrial Engineering University of Naples"FedericoⅡ"Naples 80125Italy

The potential benefits of hybrid-electric or all-electric propulsion have led to a growing interest in this topic over the past *** design of propulsion systems and innovative configurations has been extensively discussed in literature,but steps towards higher levels of technological readiness,optimisation algorithms based on reliable weight estimation and simulationbased mission analysis are *** paper focuses on the integration of a method for evaluating the lateral-directional controllability of an aircraft within a design chain that integrates aero-propulsive interactions,accurate modelling of the fuel system,and mid-fidelity estimation of the structural ***,the present work proposes a strategy for powerplant management in scenarios with an inoperative chain *** of hybrid-electric propulsion on the design of the vertical tail plane are evaluated involving the analysis of multiple failure scenarios and certification *** proposed application concerns a commuter aircraft.

关键词： Distributed electric propulsion Failure management Fault-tolerance analysis Hybrid-electric aircraft Lateral-directional controllability

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Compressing deep-quaternion neural networks with targeted regularisation

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CAAI Transactions on Intelligence Technology 2020年第3期5卷 172-176页

作者： Riccardo Vecchi Simone Scardapane danilo Comminiello Aurelio Uncini Department of Information Engineering Electronics and Telecommunications(DIET)‘Sapienza’University of RomeRoma Lazio 00185Italy

In recent years,hyper-complex deep networks(such as complex-valued and quaternion-valued neural networks–QVNNs)have received a renewed interest in the *** find applications in multiple fields,ranging from image reconstruction to 3D audio *** to their real-valued counterparts,quaternion neural networks require custom regularisation strategies to avoid *** addition,for many real-world applications and embedded implementations,there is the need of designing sufficiently compact networks,with few weights and ***,the problem of regularising and/or sparsifying QVNNs has not been properly addressed in the literature as of *** this study,the authors show how to address both problems by designing targeted regularisation strategies,which can minimise the number of connections and neurons of the network during *** this end,they investigate two extensions of ℓ1 and structured regularisations to the quaternion *** the authors’experimental evaluation,they show that these tailored strategies significantly outperform classical(realvalued)regularisation approaches,resulting in small networks especially suitable for low-power and real-time applications.

关键词： networks neural valued

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Hyperledger fabric platform for healthcare trust relations—Proof-of-Concept

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Blockchain(Research and Applications) 2023年第4期4卷 63-74页

作者： Aleksandar Nedakovic Anton Hasselgren Katina Kralevska danilo Gligoroski Department of Information Security and Communication Technology Norwegian University of Science and Technology(NTNU)7034 TrondheimNorway Department of Neuromedicine and Movement Science Norwegian University of Science and Technology(NTNU)7034 TrondheimNorway

In recent years,blockchain technologies have expanded from the finance field to other areas that rely on trustbased *** healthcare industry represents one such area,as digital transformation disrupts relationships between patients,healthcare professionals,and healthcare *** and healthcare institutes lack a proficient tool to verify the credentials of medical professionals in a digital ***,healthcare professionals lack a tool where they are in control over their *** first contribution of this paper is a proposal of a solution that leverages the private permissioned Hyperledger Fabric blockchain and smart contracts to provide a source of transparent trust for relationships within the healthcare ***,we pave the ground for GDPR compliance by storing only the hash values on the ***,we solve the problem of patient authentication by utilizing cryptographic ***,we prove the usability of the proposed solution by implementing a user interface and creating a live deployment.

关键词： Blockchain Hyperledger fabric Health systems Distributed ledger

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