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Homotypic Vacuole Fusion Requires VTI11 and Is Regulated by Phosphoinositides

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molecular Plant 2014年第6期7卷 1026-1040页

作者： Sang Won Han maria fernanda Rodriguez-Welsh marcela Rojas-Pierce Department of Plant and microbial Biology North Carolina State University Raleigh NC 27695 USA

most plant cells contain a large central vacuole that is essential to maintain cellular turgor. We report a new mutant allele of VTI11 that implicates the SNARE protein VTI11 in homotypic fusion of protein storage and lytic vacuoles. Fusion of the multiple vacuoles present in vtill mutants could be induced by treatment with Wortmannin and LY294002, which are inhibitors of Phosphatidylinositol 3-Kinase （PI3K）. We provide evidence that Phosphatidylinositol 3-Phosphate （Ptdlns（3）P） regulates vacuole fusion in vtill mutants, and that fusion of these vacuoles requires intact microtubules and actin filaments. Finally, we show that Wortmannin also induced the fusion of guard cell vacuoles in fava beans, where vacuoles are naturally fragmented after ABA-induced stomata closure. These results suggest a ubiquitous role of phosphoinositides in vacuole fusion, both during the development of the large central vacuole and during the dynamic vacuole remodeling that occurs as part of stomata movements.

关键词： vacuole SNARE phosphoinositides membrane fusion Wortmannin.

Inositol 1,4,5-trisphosphate receptor in the liver:Expression and function

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World Journal of Gastroenterology 2019年第44期25卷 6483-6494页

作者： fernanda de Oliveira Lemos Rodrigo m Florentino Antonio Carlos melo Lima Filho marcone Loiola dos Santos m Fatima Leite Department of Physiology and Biophysics Universidade Federal de Minas GeraisBelo HorizonteMinas Gerais 31270-901Brazil Department of Cell Biology Universidade Federal de Minas GeraisBelo HorizonteMinas Gerais 31270-901Brazil

The liver is a complex organ that performs several functions to maintain *** functions are modulated by calcium,a second messenger that regulates several intracellular *** hepatocytes and cholangiocytes,which are the epithelial cell types in the liver,inositol 1,4,5-trisphosphate(InsP3)receptors(ITPR)are the only intracellular calcium release *** isoforms of the ITPR have been described,named type 1,type 2 and type *** ITPR isoforms are differentially expressed in liver cells where they regulate distinct physiological *** in the expression level of these receptors correlate with several liver diseases and hepatic *** this review,we highlight how the expression level,modulation,and localization of ITPR isoforms in hepatocytes and cholangiocytes play a role in hepatic homeostasis and liver pathology.

关键词： Inositol 1,4,5-trisphosphate receptor Liver Calcium signaling Hepatocytes and cholangiocytes

Effects of maternal dietary omega-3 polyunsaturated fatty acids and methionine during late gestation on fetal growth,DNA methylation,and mRNA relative expression of genes associated with the inflammatory response,lipid metabolism and DNA methylation in placenta and offspring’s liver in sheep

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Journal of Animal Science and Biotechnology 2021年第2期12卷 611-625页

作者： milca Rosa Velazquez fernanda Batistel Juan manuel Pinos Rodriguez Alejandro Enrique Relling Facultad de medicina Veterinaria y Zootecnia Universidad Veracruzana91710 VeracruzMexico Department of Animal Science Ohio Agricultural Research and Development Center(OARDC)The Ohio State University114 Gerlaugh Hall1680 Madison AveWoosterOH 44691USA Department of Animal Dairy and Veterinary SciencesUtah State UniversityLoganUT 84322USA

Background:Omega-3 PUFA or methionine(met)supply during gestation alters offspring ***,the effect of both nutrients on fetal development has not been *** objective was to determine the effects of supplementation of these two nutrients during late gestation on fetal growth,DNA methylation,and mRNA expression of genes associated with the inflammatory response,and DNA ***(n=5/treatment)were fed from day 100 to 145 of gestation one of the following treatments:1)basal diet(NS)without fatty acids(FS)or methionine(mS)supplementation;2)FS(10 g/kg Ca salts,source omega-3 PUFA);3)mS(1 g/kg rumen protected methionine);and 4)FS and mS(FS-mS).On day 145,ewes were euthanized,and data from dams and fetus was ***(cotyledon),fetal liver,and blood samples were ***:A treatments interaction on fetal liver weight,ewe body weight and body condition score(BCS)was observed;FS-mS were heavier(PmS,and FS-mS ewes had a better(P=0.02)BCS than *** increased(P=0.03)ewe plasma glucose *** liver global DNA methylation increased(Pments modify the mRNA relative expression on some of the genes *** the fetal liver,FS increased(P=0.04)the mRNA relative expression of arachidonate-5-lipoxygenase-activatingprotein and tended to decrease(P=0.06)***,mS decreased(P=0.04)DNA-methyltransferase-1 and tended to decrease(P=0.08)free-fatty-acid-receptor-1 mRNA relative ***,FS-mS decreased mRNA relative expression of tumor-necrosis-factor-alpha(P=0.05),peroxisomeproliferator-activated-receptor-delta(P=0.03)and gamma(P=0.04),tended to decrease(P≤0.09)interleukin-6,fatty-acid-transport-protein-1,and delta-5-desaturase,and increased adenosylhomocysteinase(P=0.04)mRNA relative *** cotyledon,FS tended to decrease fatty acid binding protein 4(P=0.09)mRNA relative ***:Omega-3 PUFA and met s

关键词： Fat supplementation Fetal programming maternal nutrition Nutrients Rumen protected methionine

The global climate monitor system:from climate data-handling to knowledge dissemination

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International Journal of Digital Earth 2019年第4期12卷 394-414页

作者： Juan mariano Camarillo-Naranjo José Ignacio Álvarez-Francoso Natalia Limones-Rodríguez maría fernanda Pita-López mónica Aguilar-Alba Department of Physical Geography and Regional Analysis University of SevilleSevilleSpain Department of Human Geography University of SevilleSevilleSpain

This paper summarizes our work on building a data model and a geovisualization tool that provides access to global climate data:the Global Climate monitor Web *** to this viewer,a complete set of climate-environmental indicators capable of displaying climate patterns on a global scale that is accessible to any potential user(scientists and laypeople)will be built and published using the same online *** data currently available correspond to the CRU TS3.21 version of the Climate Research Unit(University of East Anglia)database–a product that provides data at a spatial resolution of half of a degree in latitude and longitude,spanning January 1901 to December 2012,on a monthly *** January 2013,the datasets feeding the system have been the GHCN-CAmS temperature dataset and the Global Precipitation Climatology Centre(GPCC)First Guess precipitation ***,hydrologists,planners and non-experts users such as media workers,policymakers,non-profit organizations,teachers or students,can access useful climatological information through the Global Climate monitor system.

关键词： Geovisualization climate indicators global climate databases open-source software

Experimental approaches to select tree species for forest restoration:effects of light,water availability and interspecific competition in degraded areas

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Journal of Forestry Research 2022年第4期33卷 1197-1207页

作者： Johanna Croce Ernesto I.Badano Carolina B.Trigo fernanda martinez-Galvez Andrés Tálamo Laboratorio de Ecología Aplicada a la Conservación Instituto de Bio y Geociencias del Noroeste ArgentinoConsejo de Investigaciones Científi cas y Técnicas-Universidad Nacional de SaltaAvenida Bolivia 51504400 SaltaArgentina IPICYT/División de Ciencias Ambientales Instituto Potosino de Investigación Científi ca y TecnológicaCamino a la Presa San José2055Colonia Lomas 4^(a)Sección78216 San Luis PotosíMéxico

Knowing what native trees can recr uit on degraded areas allows selecting the best species to restore these ***,as this information is not often available,experimentation is required before large-scale *** study used ex situ experiments to make these decisions on *** with r-strategist plants,excessive solar radiation and water shortage commonly impair tree recruitment in open *** experiments focused on the interactions among these factors and were conducted with three pioneer species from seasonally dry forests of northwest Argentina,Anadenanthera colubrina,Ceiba chodatii and Jacaranda *** of each species were sown at two light levels(sunlight/shade),two rainfall levels(full/reduced)and two levels of interspecific competition(with/without competitor)in a tree *** emergence and survival were monitored over a year and the results indicate that species differentially respond to varying levels of light and *** of *** tolerated water shortages under elevated solar radiation,which are desirable features for forest *** of *** tolerated shade and drought,suggesting that they require shading for establishing in open ***,*** seedlings neither tolerated full sunlight nor water shortages,suggesting that this species requires shading and regular watering if used in *** of the effects of light and water,the survival of all species was reduced by interspecific *** results highlight the importance of experimentation for selecting the best species for forest restoration and can enhance the cost/benefit ratios of these actions.

关键词： Common-garden experiments Drought Ecological restoration Interspecific competition Seedling survival Shade

Unraveling the morphological patterns of a subantarctic eelpout:a geometric morphometric approach

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Integrative Zoology 2023年第2期18卷 372-384页

作者： fernanda S.ORREGO mathias HÜNE Hugo A.BENÍTEZ mauricio F.LANDAETA Laboratorio de Ictiología e Interacciones Biofísicas(LABITI) Instituto de BiologíaFacultad de CienciasUniversidad de ValparaísoValparaísoChile Centro de Investigación para la Conservación de los Ecosistemas Australes(ICEA) Punta ArenasChile Laboratorio de Ecología y morfometría Evolutiva Centro de Investigación de Estudios Avanzados del MauleUniversidad Católica del MauleTalcaChile Centro de Observación marino para Estudios de Riesgos del Ambiente Costero(COSTA-R) Universidad de ValparaísoViña del MarChile millennium Nucleus for Ecology and Conservation of Temperate mesophotic Reef Ecosystems(NUTmE) millennium Institute Biodiversity of Antarctic and Subantarctic Ecosystems(BASE) SantiagoChile

Phenotypic variation in organisms depends on the genotype and the environmental constraints of the habitat that they ***,for marine species inhabiting contrasting aquatic conditions,it is expected toﬁnd covari-ation between the shape and its spatial *** studied the morphology of the head and cephalic sensory canals of the eelpout Austrolycus depressiceps(4.5–22.5 cm TL)across its latitudinal distribution in South Paciﬁc(45°S–55°S).Geometric morphometric analyses show that the shape varied from individuals with larger snout and an extended suborbital canal to individuals with shorter snouts and frontally compressed suborbital *** was size variation across the sampled populations,but that size does not have a clear latitudinal *** 8%of the shape variation relates to this size variation(allometry),represented by a decrease in the relative size of the eye,and a depression of the posterior margin of the *** were spatial differences in the shape of the head,but these differences were probably caused by allometric ***,2 of the canals of the cephalic lateralis pores and the head shape showed modularity in its *** study shows that the morphology of marineﬁsh with a shallow distribution varies across distances of hundreds of kilometers(i.e.,phenotypic modulation).

关键词： Austrolycus depressiceps geometric morphometrics morphology phenotype spatial distribution

Chondrogenic potential of mesenchymal stem cells from horses using a magnetic 3D cell culture system

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World Journal of Stem Cells 2021年第6期13卷 645-658页

作者： Joice Fülber fernanda R Agreste Sarah R T Seidel Eric D P Sotelo Ângela P Barbosa Yara m michelacci Raquel Y A Baccarin Departamento de Clínica médica Medicina Veterinária e ZootecniaUniversidade de São PauloSão Paulo 05506-270Brazil Departamento de Bioquímica Escola Paulista de MedicinaUniversidade Federal de São PauloSão Paulo 04044-020Brazil

BACKGROUND mesenchymal stem cells(mSCs)represent a promising therapy for the treatment of equine joint diseases,studied due to their possible immunomodulatory characteristics and regenerative ***,the source of most suitable mSCs for producing cartilage for regenerative processes in conjunction with biomaterials for an enhanced function is yet to be *** To compare the chondrogenicity of mSCs derived from synovial fluid,bone marrow,and adipose tissue of horses,using the aggrecan *** mSCs from ten horses were cultured,phenotypic characterization was done with antibodies CD90,CD44 and CD34 and were differentiated into *** 3D cell culture system in which biocompatible nanoparticles consisting of gold,iron oxide,and poly-L-lysine were added to the cells,and they were forced by magnets to form one *** microspheroids were exposed to a commercial culture medium for 4 d,7 d,14 d,and 21 *** extraction was performed,and aggrecan was quantified by enzyme-linked immunosorbent *** sulfate and aggrecan in the microspheroids were identified and localized by *** All cultured cells showed fibroblast-like appearance,the ability to adhere to the plastic surface,and were positive for CD44 and CD90,thus confirming the characteristics and morphology of *** soluble protein concentrations were higher in the microspheroids derived from adipose *** aggrecan concentration and the ratio of aggrecan to soluble proteins were higher in microspheroids derived from synovial fluid than in those derived from bone marrow,thereby showing chondrogenic *** from all sources expressed aggrecan and keratan sulfate when observed using confocal immunofluorescence *** sources of mSCs can synthesize aggrecan,however,mSCs from synovial fluid and adipose tissue have demonstrated better biocompatibility in a 3D environment,thus suggesting chondrogenic superiority

关键词： Cellular therapy Chondrocytes Aggrecan Keratan sulfate

Insights into the swelling process and drug release mechanisms from cross-linked pectin/high amylose starch matrices

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Asian Journal of Pharmaceutical Sciences 2014年第1期9卷 27-34页

作者： fernanda m.Carbinatto Ana Do´ris de Castro Raul C.Evangelista Beatriz S.F.Cury Graduate Program in Pharmaceutical Sciences and Department of Drugs and Pharmaceuticals School of Pharmaceutical SciencesSa˜o Paulo State UniversityBrazil

Cross-linked pectin/high amylose mixtures were evaluated as a new excipient for matrix tablets formulations,since the mixing of polymers and cross-linking reaction represent rational tools to reach materials with modulated and specific properties that meet specific therapeutic ***:In this work the influence of polymer ratio and cross-linking process on the swelling and the mechanism driving the drug release from swellable matrix tablets prepared with this excipient was ***:Cross-linked samples were characterized by their micromeritic properties(size and shape,density,angle of repose and flow rate)and liquid uptake *** tablets were evaluated according their physical properties and the drug release rates and mechanisms were also ***:Cross-linked samples demonstrated size homogeneity and irregular shape,with liquid uptake ability insensible to ***-linking process of samples allowed the control of drug release rates and the drug release mechanism was influenced by both polymer ratio and cross-linking *** drug release of samples with minor proportion of pectin was driven by an anomalous transport and the increase of the pectin proportion contributed to the erosion of the ***:The cross-linked mixtures of high amylose and pectin showed a suitable excipient for slowing the drug release rates.

关键词： High amylose starch Pectin Cross-linking Swelling Drug delivery mechanism

Current and novel approaches in the pharmacological treatment of hepatocellular carcinoma

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World Journal of Gastroenterology 2023年第17期29卷 2571-2599页

作者： fernanda Villarruel-melquiades maría Eugenia mendoza-Garrido Claudia m García-Cuellar Yesennia Sánchez-Pérez Julio Isael Pérez-Carreón Javier Camacho Departamento de Farmacología Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional(CINVESTAV-IPN)Mexico City 07360Mexico Departamento de Fisiología Biofísica y NeurocienciasCentro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional(CINVESTAVIPN)Mexico City 07360Mexico Subdirección de Investigación Básica Instituto Nacional de Cancerología(INCan)Mexico City 14080Mexico Instituto Nacional de medicina Genómica Instituto Nacional de Medicina Genómica(INMEGEN)Mexico City 14610Mexico

Hepatocellular carcinoma(HCC)is one of the most lethal malignant tumours *** mortality-to-incidence ratio is up to 91.6%in many countries,representing the third leading cause of cancer-related *** drugs,including the multikinase inhibitors sorafenib and lenvatinib,are first-line drugs used in HCC ***,these therapies are ineffective in most cases due to late diagnosis and the development of tumour ***,novel pharmacological alternatives are urgently *** instance,immune checkpoint inhibitors have provided new approaches targeting cells of the immune ***,monoclonal antibodies against programmed cell death-1 have shown benefits in HCC *** addition,drug combinations,including first-line treatment and immunotherapy,as well as drug repurposing,are promising novel therapeutic ***,we review the current and novel pharmacological approaches to fight *** studies,as well as approved and ongoing clinical trials for liver cancer treatment,are *** pharmacological opportunities analysed here should lead to significant improvement in HCC therapy.

关键词： Liver cancer Systemic therapy Immunotherapy Drug repurposing Drug combinations Hepatocellular carcinoma