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Keratinocyte growth factor-2 and autologous serum potentiate the regenerative effect of mesenchymal stem cells in cornea damage in rats

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International Journal of Ophthalmology(English edition) 2014年第2期7卷 211-219页

作者： ferda alpaslan pinarli Gülsen Okten Umit Beden Tunc Fisgin Mehmet Kefeli Nurten Kara Feride Duru Leman Tomak Department of Medical Biology and Genetics Faculty of Medicine Ondokuz Mayis University Department of Ophthalmology Faculty of MedicineOndokuz Mayis University Department of Pediatric Hematology Faculty of MedicineOndokuz Mayis University Department of Pathology Faculty of Medicine Ondokuz Mayis University Department of Medical Biostatistics Faculty of MedicineOndokuz Mayis University

AIM:To investigate the healing process after severe corneal epithelial damage in rats treated with mesenchymal stem cells(MSCs)cultured with or without keratinocyte growth factor(KGF-2)and autologous serum(AS)on amniotic membrane(AM).Many patients are blind and devastated by severe ocular surface diseases due to limbal stem cell *** marrow-derived MSCs are potential sources for cellbased tissue engineering to repair or replace the corneal tissue,having the potential to differentiate to epithelial ***:The study included 5 groups each including 10 female'Sprague Dawley'rats in addition to20 male rats used as bone marrow *** I rats received AM+MSCs,Group II rats AM+MSCs cultured with KGF-2,Group III rats AM+MSCs cultured with KGF-2+AS,Group IV rats only AM and Group V rats,*** was derived from blood drawn from male rats and bone marrow was obtained from the femur and tibia bones of the same *** effect was evaluated with clinical,histopathological and immunohistochemical *** engraftment was demonstrated via detection of donor genotype(Y+)in the recipient tissue(X)with polymerase chain ***:Corneal healing was significantly better in Groups I-III rats treated with MSC transplantation compared to Group IV and Group V rats with supportive treatment *** best results were obtained in Group III rats with 90%transparency,70%lack of neovascularization,and 100%epithelium damage limited to less than 1/4 of ***:We suggest that culture of MSCs with KGF-2 and AS on AM is effective in corneal repair in case of irreversible damage to limbal stem cells.

关键词： corneal wound healing mesenchymal stem cells keratinocyte growth factor-2 autologous serum amniotic membrane

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