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Villin Family Members Associated with Multiple Stress Responses in Cotton

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Phyton-International Journal of Experimental Botany 2021年第6期90卷 1645-1660页

作者： fenni Lv Sen Wang Ruiping Tian Peng Wang kang Liu State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing210095China Institute of Botany Jiangsu Province and Chinese Academy of SciencesNanjing210014China Institute of Food Crops Jiangsu Academy of Agricultural SciencesNanjing210014China

Villin(VLN)is considered to be one of the most important actin-binding proteins,participates in modulating the actin cytoskeleton dynamics,plays essential role in plant development and resisting adverse ***,systematic studies of the VLN gene family have not been reported in cotton(Gossypium).In this study,14 GhVLNs were identified in *** GhVLN genes were distributed in 6 A-subgenome chromosomes and 6 D-subgenome chromosomes of the allotetraploid upland cotton and classified into three phylogenetical groups based on the classification model of *** addition,the 14 GhVLN genes have highly conserved gene structure and motif *** number of introns was ranged from 18 to 22 and the length of protein sequences was varied from 901 to *** gelsolin homology domains,G1–G6,and villin headpiece domain,VHP,were existed in all GhVLNs with the exception of two VLNs(GhVLN6 and GhVLN13)which lacked ***-elements analysis revealed that the promoter regions of GhVLNs contained various light related components and also elements responsible for phytohormones and stresses response,indicating that,when subjected to those adverse environments,cotton plants may activate the response system by targeting VLN genes to survive the *** showed that the GhVLN genes exhibited various expression patterns,some were accumulated in certain tissues,root,petal,stamen or elongating fibers,and some were obviously induced by environmental *** GhVLN3 and GhVLN10 were highly and preferentially expressed in elongating fibers and distinctly upregulated by abiotic(salt,PEG,cold and heat)and biotic(Verticillium dahliae V991)*** study may provide useful information for biological function identification of GhVLN genes and gene resources for creating high-quality and various resistant cotton germplasms.

关键词： Gossypium hirsutum villin proteins cis-elements abiotic and biotic stresses

A mathematical method to calculate efficiency of BF_3 detectors

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Nuclear Science and Techniques 2009年第3期20卷 181-183页

作者： SI fenni HU Qingyuan PENG Taiping Institute of Nuclear Physics and Chemistry China Academy of Engineering Physics Mianyang 621900 China

In order to calculate absolute efficiency of the BF3 detector, MCNP/4C code is applied to calculate relative efficiency of the BF3 detector first, and then absolute efficiency is figured out through mathematical techniques. Finally an energy response curve of the BF3 detector for 1~20 MeV neutrons is derived. It turns out that efficiency of BF3 detector are relatively uniform for 2~16 MeV neutrons.

关键词： BF3 中子加速器核技术

Optimization of energy exchange in microgrid networks: a coalition formation approach

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Protection and Control of Modern Power Systems 2019年第1期4卷 322-331页

作者： Chaimaa Essayeh Mohammed Raiss El fenni Hamza Dahmouni Department of Communication Systems INPTRabatMadinat Al IrfaneMorocco

In this paper, we elaborate a new strategy based on cooperative game theory models to encourage and manage the interactions in a MicroGrid network. The proposed strategy optimizes the cooperation and the energy exchange in a distributed μGrid network. The strategy consists of a two stage algorithm: Coalition formation algorithm which was specifically created to approximate the optimal set of coalitions that return considerable savings. And the Matching game to manage the energy exchange inside each coalition. The performance of our strategy was verified through simulations. These latter show that the losses can be considerably decreased by the use of the proposed strategy: the rate of the loss reduction can reach up to 20% if the two stages are applied on the network. Moreover, the strategy proved to have a fast convergence which makes it operational for real implemented networks.

关键词： MicroGrid network Energy optimization Cooperative Game theory Matching game Coalition formation

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高通量测序分析乳酸菌菌群多样性的研究进展

现代食品 2024年第11期30卷 59-65页

作者：刘辉杜芬妮郑智汉陈波浙江一鸣食品股份有限公司浙江温州325400

乳酸菌被广泛用于生产各种食品和原料饲料,对发酵产品的风味和质地有特殊贡献。此外,特定的乳酸菌菌株因其对消费者健康的促进作用而被视为益生菌。近年来,随着测序技术的蓬勃发展,对乳酸菌基因组进行测序以全面了解乳酸菌代谢产物的多... 详细信息

乳酸菌被广泛用于生产各种食品和原料饲料,对发酵产品的风味和质地有特殊贡献。此外,特定的乳酸菌菌株因其对消费者健康的促进作用而被视为益生菌。近年来,随着测序技术的蓬勃发展,对乳酸菌基因组进行测序以全面了解乳酸菌代谢产物的多样性以及预测相关基因的功能,为乳酸菌的挖掘与应用带来了前所未有的机会。本文综述了高通量测序技术在乳酸菌多样性分析、功能基因检测方面的应用,为乳酸菌的功能性开发、代谢产物分析以及应用方向提供了参考。

关键词：高通量乳酸菌基因组多样性

铁线莲属萼片荧光定量PCR内参基因的筛选和评价

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分子植物育种 2024年第8期22卷 2575-2582页

作者：李桥王淑安王鹏吕芬妮汪庆李林芳李亚江苏省中国科学院植物研究所南京中山植物园南京210014

以不同花色的铁线莲属(Clematis L.)栽培品种‘恬美’(C.‘Sympatia’)、‘罗兰’(C.‘Rooran’)、‘仙女座’(C.‘Andromeda’)、‘阿拉贝拉’(C.‘Arabella’)、‘普鲁吐斯’(C.‘Proteus’)以及‘路易斯罗维’(C.‘Louise Rowe’)初... 详细信息

以不同花色的铁线莲属(Clematis L.)栽培品种‘恬美’(C.‘Sympatia’)、‘罗兰’(C.‘Rooran’)、‘仙女座’(C.‘Andromeda’)、‘阿拉贝拉’(C.‘Arabella’)、‘普鲁吐斯’(C.‘Proteus’)以及‘路易斯罗维’(C.‘Louise Rowe’)初花时期的萼片为研究材料,利用RT-qPCR技术检测Atpb、Arp7、Ubc34、Wit1、Cxip4、Cyp71a1和Cyp35a1等7个候选内参基因的mRNA表达情况,并利用软件geNorm、NormFinder、BestKeeper和RefFinder对7个候选内参基因的稳定性进行综合评估。结果表明,内参基因表达差异显著;利用geNorm软件计算结果为基因Atpb与Cxip4最稳定;利用NormFinder软件计算结果为基因Cxip4最稳定;而BestKeeper软件计算出的最稳定的候选内参基因为Arp7,其次为Atpb;按这3个软件的计算结果,Cyp71a1与Cyp35a1的稳定性均最差,不适合作为内参基因;RefFinder软件分析的最佳的候选内参基因为Arp7。经综合分析,这几个铁线莲品种萼片基因表达的适宜内参基因是Arp7和Atpb。

关键词：铁线莲属实时荧光定量PCR 内参基因

Microelectrode Recording of Tissue Neural Oscillations for a Bionic Olfactory Biosensor

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Journal of Bionic Engineering 2012年第4期9卷 494-500页

作者： Qingjun Liu fenni Zhang Ning Hu Hua Wang Kuen Jimmy Hsia Ping Wang Biosensor National Special Laboratory Department of Biomedical Engineering Zhejiang University Hangzhou 310027 P. R. China Key Laboratory of Biomedical Engineering of Education Ministry Department of Biomedical Engineering Zhejiang University Hangzhou 310027 P. R. China Department of Mechanical Science and Engineering University of Illinois at Urbana-Champaign Urbana IL 61801 USA

In olfactory research, neural oscillations exhibit excellent temporal regularity, which are functional and necessary at thephysiological and cognitive levels. In this paper, we employed a bionic tissue biosensor which treats intact epithelium as sensing element to record the olfactory oscillations extracellularly. After being stimulated by odorant of butanedione, the olfactory receptor neurons generated different kinds of oscillations, which can be described as pulse firing oscillation, transient firing oscillation, superposed firing oscillation, and sustained firing oscillation, according to their temporal appearances respectively. With a time-frequency analysis of sonogram, the oscillations also demonstrated different frequency properties, such as δ, θ, α, β and γ oscillations. The results suggest that the bionic biosensor cooperated with sonogram analysis can well improve the in- vestigation of olfactory oscillations, and provide a novel model for artificial olfaetion sensor design.

关键词： bionic sensor olfactory epithelium microelectrode array (MEA) neural oscillation olfactory bioscnsor

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基于胚性愈伤诱变的楸树耐盐种质创制及评价

分子植物育种 2024年第13期22卷 4357-4363页

作者：高露璐王鹏顾姣姣姜聖姬李亚赵明杨吕芬妮江苏省中国科学院植物研究所南京210014 睢宁县自然资源和规划局徐州221200

楸树(Catalpa bungei)是中国特有的落叶硬木类珍贵树种,具有速生、耐盐碱、材质优良等优点,是沿海滩涂和内陆盐碱地土壤修复和防护的优良树种。创制新的耐盐种质,培育耐盐新品种,提高楸树耐盐能力,是目前急需解决的科学问题。本研究以... 详细信息

楸树(Catalpa bungei)是中国特有的落叶硬木类珍贵树种,具有速生、耐盐碱、材质优良等优点,是沿海滩涂和内陆盐碱地土壤修复和防护的优良树种。创制新的耐盐种质,培育耐盐新品种,提高楸树耐盐能力,是目前急需解决的科学问题。本研究以楸树品系‘钟山5号’胚性愈伤为材料,利用^(137)Cs-γ射线结合耐盐定向筛选创制新的耐盐种质。结果表明,随着辐射剂量、盐浓度的逐渐增加,胚性愈伤的褐化率均逐渐增加,增殖率降低,因此,定向诱变最适的辐射剂量为40 Gy,最适的盐浓度为300 mmol/L NaCl。将用40 Gy处理后的愈伤放置在含300 mmol/L NaCl的再生培养基中,获得65株楸树再生幼苗,又经过苗期二级耐盐性评价获得3份耐盐的楸树新种质。本研究不仅为楸树耐盐品种培育提供新的种质材料,也为楸树耐盐基因克隆及机理研究提供科学依据。

关键词：胚性愈伤 ^(137)Cs-γ射线种质创制耐盐评价

Bioelectronic modulation of single-wavelength localized surface plasmon resonance (LSPR) for the detection of electroactive biomolecules

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Chinese Chemical Letters 2022年第6期33卷 3144-3150页

作者： Zetao Chen fenni Zhang Yanli Lu Yaru Li Guang Liu Jianzhen Shan Qingjun Liu Biosensor National Special Laboratory Key Laboratory for Biomedical Engineering of Education MinistryDepartment of Biomedical EngineeringZhejiang UniversityHangzhou 310027China The First Affiliated Hospital Zhejiang University School of MedicineHangzhou 310027China

The simplification of localized surface plasmon resonance(LSPR) detection can further promote the development of optical biosensing application in point-of-care testing. In this study, we proposed a simple light emitting diode(LED) based single-wavelength LSPR sensor modulated with bio-electron transfers for the detection of electroactive biomolecules. Indium tin oxide electrode loaded with nanocomposites of polyaniline coated gold nanorod was used as LSPR chip, and the applied electric potential was scanned at the LSPR chip for single-wavelength LSPR biosensing. Under the scanning of applied potentials, biological electron transfer of redox reaction was employed to demonstrate the bioelectronic modulation of single-wavelength LSPR for selective electroactive biomolecule detection. Without any additional recognition material, electroactive biomolecules uric acid and dopamine were detected directly with a sensitivity of 5.05 μmol/L and 7.11 μmol/L at their specific oxidation potentials, respectively. With the simplified optical configuration and selective bioelectronic modulation, the single-wavelength LSPR sensor is promising for the development of simple, low-cost, and high specificity optical biosensor for point-of-care testing of electroactive biomolecules.

关键词： LSPR LED light Bio-electron transfer Electroactive biomolecule Biosensor

纳米比亚中等学校中的职业教育 ——奥希科托地区实施情况公平性分析

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纳米比亚中等学校中的职业教育 ——奥希科托地区实施情况公平性...

作者： fenni Jambeko Namutenya 华东师范大学

学位级别：硕士

教育不公会减缓国家的经济增长速度。学习能力低于平均水平和学习速度缓慢的学生因无法获得所需的教育资源而导致他们无法达到最佳学习效果。这些学生没能获得必要技能以在毕业后应对生活。其结果是,他们收入不高,财富难以积累,也因此... 详细信息

教育不公会减缓国家的经济增长速度。学习能力低于平均水平和学习速度缓慢的学生因无法获得所需的教育资源而导致他们无法达到最佳学习效果。这些学生没能获得必要技能以在毕业后应对生活。其结果是,他们收入不高,财富难以积累,也因此无法负担孩子去优质学校学习的费用。这种状况以一种结构性不平等的循环方式持续存在,最终伤害整个社会(Amedeo,2021)。纳米比亚的大多数学生,无论他们的学习能力如何,只能选择跟随学校的安排学习。这一情况影响了学生的学业表现,减少了有资格进入高等教育机构学习的学生数量,最终导致纳米比亚青年失业率居高不下。因此,本研究对纳米比亚奥希科托地区中等学校中的职业教育的实施情况进行了分析,以证实学习有困难的中学生在接受职业教育方面是否受到公平对待。本研究采用混合研究方法以证实纳米比亚奥希科托地区中等学校中的职业教育现状,明确中等学校中的职业教育公平程度,弄清影响中等学校职业教育公平实施的挑战因素。数据收集来自于教育部文件以及对校长、低水平学生和失学青年所做的问卷调查。通过质化和量化数据分析,本研究结果显示在奥希科托地区的中等学校,学生在接受职业教育方面存在高度的不平等,大多数学生仅以农业科学作为主要的职业预科课程学习。该地区只有少数学校开设其他职业科目,有些学校根本没有开设任何职业预科课程,而有一些职业预科课程在所有学校都没有开设。Data analysis was done for研究建议所有教育利益相关者和政策制定者都急需进行干预,急需制定更加实际的实施计划和或战略,以有效实施纳米比亚现有的、经过最新改革的国家基础教育课程。

关键词：教育公平职业教育中学失学青年