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The soybean PLATZ transcription factor GmPLATZ17 suppresses drought tolerance by interfering with stress-associated gene regulation of GmDREB5

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The Crop Journal 2022年第4期10卷 1014-1025页

作者： Juanying Zhao Lei Zheng Jitong Wei Yixuan wang Jun Chen Yongbin Zhou Ming Chen Fengzhi wang Youzhi Ma Zhao-Shi Xu Institute of Crop Sciences Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic ImprovementKey Laboratory of Biology and Genetic Improvement of Triticeae CropsMinistry of AgricultureBeijing 100081China Hebei Key Laboratory of Crop Salt-alkali Stress Tolerance Evaluation and Genetic Improvement/Cangzhou Academy of Agriculture and Forestry Sciences Cangzhou 061000HebeiChina

Plant AT-rich sequence and zinc binding(PLATZ)transcription factors are a class of plant specific zincdependent DNA-binding proteins that function in abiotic stress response and plant development.In this study,31 GmPLATZ genes were identified in soybean.GmPLATZ17 was down-regulated by drought and exogenous abscisic acid.Transgenic Arabidopsis and soybean hairy roots overexpressing GmPLATZ17 showed drought sensitivity and inhibition of stress-associated gene transcription.In contrast,suppressed expression of GmPLATZ17 led to increased drought tolerance in transgenic soybean hairy roots.The GmPLATZ17 protein was verified to interact physically with the GmDREB5 transcription factor,and overexpression of GmDREB5 increased drought tolerance in soybean hairy roots.Interaction of GmPLATZ17 with GmDREB5 was shown to interfere with the DRE-binding activity of GmDREB5,suppressing downstream stress-associated gene expression.These results show that GmPLATZ17 inhibits drought tolerance by interacting with GmDREB5.This study sheds light on PLATZ transcription factors and the function of GmPLATZ17 in regulating drought sensitivity.

关键词： Soybean PLATZ transcription factor Protein interaction Regulation mechanism Drought response

Design,synthesis and biological evaluation of fluorescent derivatives of ursolic acid in living cells

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Chinese Chemical Letters 2024年第5期35卷 287-289页

作者： Wenyi Mei Lijuan Xie Xiaodong Zhang Cunjian Shi Fengzhi wang Qiqi Fu Zhenjiang Zhao Honglin Li Yufang Xu Zhuo Chen Shanghai Key Laboratory of New Drug Design State Key Laboratory of Bioreactor EngineeringSchool of PharmacyEast China University of Science and TechnologyShanghai 200237China Innovation Center for AI and Drug Discovery East China Normal UniversityShanghai 200062China Lingang Laboratory Shanghai 200031China

Ursolic acid(UA)is a naturally occurring ursane triterpenoid,which exhibits a wide range of unique biological activities.To clarify its mechanism of action(MOA),a series of fluorescent derivatives of UA(5a-c)were designed and synthesized by conjugation with 7-nitrobenzo-2-oxa-1,3-diazole(NBD)fluorophore.Among them,5c exhibited similar anti-proliferative activity with UA against HCT116 cells(half maximal inhibitory concentration(IC_(50))=9.21±0.50μmol/L).Cell imaging experiment indicated that 5c was rapidly taken up in HCT116 cells in a dose and time-dependent manner.Then,5c was found to localize in endoplasmic reticulum(ER),lysosomes,and mitochondria,but not in nucleus of HCT116 cells by confocal microscopy studies.Preliminary MOA proved that UA induced autophagy with a unique intracellular distribution mechanism involving ER and lysosome.In all,our work provides new clues for revealing the molecular mechanism of UA as an antitumor agent.

关键词： Pentacyclic triterpenoid Ursolic acid Fluorescent derivatives Autophagy Apoptosis

The structure of WbnH in a near active state

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Protein & Cell 2015年第8期6卷 615-618页

作者： fengzhi Li Siwei Li Xiaofen Liu Xue Yang Peng wang Yuequan Shen State Key Laboratory of Medicinal Chemical Biology Nankai University Tianjin 300071 China College of Life Sciences Nankai University Tianjin 300071 China Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences Tianjin 300308 China Synergetic Innovation Center of Chemical Science and Engineer- ing Tianjin 300071 China

Dear Editor, Gram-negative bacteria possess a complicated membrane system that plays an essential role in interactions between bacteria and the envJronment （Reeves and wang, 2002）. The inner leaflet of the membrane is composed of various glycerophospholipids, and the outer leaflet consists primarily of lipopolysaccharide （LPS） molecules.

关键词： Corynebacterium Glutamicum Solvent Region Core Oligosaccharide Undecaprenyl Nucleotide Sugar Donor

Decomposition Method of Genetic Correlation Coefficient Based on NC Ⅱ Mating Design

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Asian Agricultural Research 2017年第1期9卷 76-77,80页

作者： Wei wang Zheng wang Fengzhi wang Songshan ZHAO Xiuzhen CUI Cangzhou Academy of Agriculture and Forestry Sciences China Petroleum Materials Corporation Life Science and Technology College Henan Institute of Science and Technology

There are different degrees of correlation between crop traits. The phenotypic correlation is decomposed into genetic and environmental correlation in quantitative genetics. In this paper,according to stochastic model of variance and covariance analysis,we calculate different genetic components,bring up a decomposition method of genetic correlation coefficient based on NC II mating design,and use examples to show analytic steps and interpret results.

关键词： Analysis of variance Analysis of covariance Stochastic model Genetic correlation coefficient

新时代斗争精神的核心要义及时代价值——从习近平总书记重要论述谈起

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浙江工业大学学报（社会科学版） 2022年第1期21卷 11-18页

作者：王凤志上海理工大学马克思主义学院上海200093

习近平总书记关于新时代斗争精神的重要论述有着深刻的时代内涵和深远的战略考量,需要结合时代特征对之进行科学解读和深化理解,厘清新时代斗争精神的内涵和特征;明晰发扬斗争精神的主体、目的和作用对象;把握好作为新时代斗争精神核心... 详细信息

习近平总书记关于新时代斗争精神的重要论述有着深刻的时代内涵和深远的战略考量,需要结合时代特征对之进行科学解读和深化理解,厘清新时代斗争精神的内涵和特征;明晰发扬斗争精神的主体、目的和作用对象;把握好作为新时代斗争精神核心要义的以人民为中心的基本立场。新时代斗争精神推动了思想意识和斗争精神元素的与时俱进,丰富了顽强拼搏语境的时代话语表达,增强了中华民族伟大复兴使命的实践动力,对于党和国家带领人民群众破除各种风险、挑战与阻力,推进伟大事业,实现中华民族伟大复兴具有深远意义和时代价值。

关键词：斗争精神核心要义时代价值

Dysbiosis of oral and gut microbiota and its association with metabolites in patients with different degrees of coronary artery stenosis

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中华医学杂志(英文版) 2024年第9期137卷 1121-1123页

作者： wang Zekun Li Long wang Chenyang wang Qian wang fengzhi Xu Xin Zhou Xuedong State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu Sichuan China Department of Cariology and Endodontics West China Hospital of Stomatology Sichuan University Chengdu Sichuan China Department of Cardiology Shanxi Provincial Key Laboratory of Cardiovascular Disease Diagnosis Treatment and Clinical Pharmacology The Second Hospital of Shanxi Medical University Taiyuan Shanxi China

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传统核心价值观的美学传播与现代张力

辽宁师范大学学报（社会科学版） 2022年第4期45卷 19-26页

作者：王凤志上海理工大学马克思主义学院上海200093

不同时代的国家和民族都非常重视社会核心价值观的认同和传播,都有自己独特的传播路径和形式。中国几千年的历史发展中积淀形成的传统核心价值观在传播和认同阶段,美学路径发挥了非常重要的作用。美学思想与核心价值观既有思想内容上的... 详细信息

不同时代的国家和民族都非常重视社会核心价值观的认同和传播,都有自己独特的传播路径和形式。中国几千年的历史发展中积淀形成的传统核心价值观在传播和认同阶段,美学路径发挥了非常重要的作用。美学思想与核心价值观既有思想内容上的相融性、理性认同上的相关性,又有实践方面的相通性。从理论上说,美学及艺术思想可以成就核心价值观的伦理目标;从实践来看,在传播技术和方式极其落后的古代、近代及新民主主义革命时期,美学及艺术形式自觉担当了传播和认同传统社会核心价值观的媒介,使之成了一份可贵的文化遗产。传统核心价值观的美学传播具有历史的适应性和时代的张力,理应承载时代赋予的价值。注重时代创新,利用现代美学载体和方法,传承、发展美学及艺术的功能,加强美育,推动现阶段社会主义核心价值观的传播和践行,促进对全体公民的思想政治教育。

关键词：核心价值观美学传播艺术

Effects of Different Water Treatment on Yield and Agronomic Traits of Wheat

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Asian Agricultural Research 2018年第3期10卷 83-87页

作者： Weiwei wang Liang YU Liya NIU Wei wang Li LU Fengzhi wang Songshan ZHAO Cangzhou Academy of Agricultural and Forestry Sciences Cangzhou 061001 China

In order to make clear the effects of water on yield and agronomic traits of wheat in Cangzhou,18 wheat cultivars from north part of Huanghuai Plain were collected for spring irrigation. The indoor and field surveys were used to analyze the effects of irrigation frequency on stem tiller situation,leaf area coefficient,yield and other traits of different wheat cultivars. The results indicated that the average yield of irrigation 1 increased by 68. 07 kg compared with that of irrigation 0,while the average yield of irrigation 2 increased by 60. 00 kg compared with that of irrigation 1. The survey of flag leaf area of wheat cultivars at the grain filling stage showed that the leaf area of irrigation 0 was the smallest( 18. 15 cm^2 on average),the leaf area of irrigation 1 was 20. 34 cm^2,and the leaf area of irrigation 2 was 24. 05 cm^2. With the increase in the irrigation times,the volume weight of wheat cultivars declined,the number of infertile spikelet dropped,and the plant height gradually increased. In conclusion,every decrease of one time of irrigation times,there will be loss of yield about 60. 00 kg.

关键词： Wheat Yield Irrigation Agronomic traits

Advances in Studies of Biological Nitrogen Saving Technology for Wheat

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Asian Agricultural Research 2018年第2期10卷 87-89,94页

作者： Liya NIU Liang YU Wei wang Weiwei wang Jing FU Fengzhi wang Songshan ZHAO Cangzhou Academy of Agricultural and Forestry Sciences Cangzhou 061001 China

This paper first described the current status of wheat nitrogen fertilizers utilization rate. Then,from balanced fertilizer application,applying slow/controlled release fertilizer,deep application of nitrogen fertilizers and graded application,controlled application amount,selection of application time according to soil properties,diagnosed application of fertilizer with instrument,addition of synergist,and precise fertilizer application,it summarized biological nitrogen saving methods and introduced prospects of future biological nitrogen saving technology.

关键词： Wheat Fertilizer utilization rate Biological nitrogen saving method