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Cytoplasmic YAP1-mediated ESCRT-III assembly promotes autophagic cell death and is ubiquitinated by NEDD4L in breast cancer

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Cancer Communications 2023年第5期43卷 582-612页

作者： Yan Guo Yuqing Cui Yangyang Li Xiaoying Jin Dandan Wang Mengxia Lei fengzhi chen Yali Liu Jinwen Xu Guanyu Yao Guangchun Zeng Xuesong chen Department of Breast Medical Oncology Harbin Medical University Cancer HospitalHarbinHeilongjiangP.R.China Department of Pathology Harbin Medical University Cancer HospitalHarbinHeilongjiangP.R.China

Background:Nuclear Yes1-associated transcriptional regulator(YAP1)promotes tumor ***,the function of cytoplasmic YAP1 in breast cancer cells and its impact on the survival of breast cancer patients remain *** research aimed to explore the biological function of cytoplasmic YAP1 in breast cancer cells and the possibility of cytoplasmic YAP1 as a predictive marker of breast cancer ***:We constructed cell mutant models,including NLS-YAP15SA(nuclear localized),YAP1S94A(incapable of binding to the TEA domain transcription factor family)and YAP1S127D(cytoplasmic localized),and used Cell Counting Kit-8(CCK-8)assays,5-ethynyl-2’-deoxyuridine(EdU)incorporation assays,and Western blotting(WB)analysis to detect cell proliferation and *** specific mechanism of cytoplasmic YAP1-mediated endosomal sorting complexes required for transport III(ESCRT-III)assembly was studied by co-immunoprecipitation,immunofluorescence staining,and WB *** gallate(EGCG)was used to simulate YAP1 retention in the cytoplasm in in vitro and in vivo experiments to study the function of cytoplasmic ***1 binding to NEDD4-like E3 ubiquitin protein ligase(NEDD4L)was identified using mass spectrometry and was verified in *** tissue microarrays were used to analyze the relationship between cytoplasmic YAP1 expression and the survival of breast cancer ***:YAP1 was mainly expressed in the cytoplasm in breast cancer *** YAP1 promoted autophagic death of breast cancer *** YAP1 bound to the ESCRT-III complex subunits charged multivesicular body protein 2B(CHMP2B)and vacuolar protein sorting 4 homolog B(VPS4B),promoting assembly of CHMP2B-VPS4B and activating autophagosome *** retained YAP1 in the cytoplasm,promoting the assembly of CHMP2B-VPS4B to promote autophagic death of breast cancer ***1 bound to NEDD4L,and NEDD4L mediated ubiquitination and degradation of *** tis

关键词： Autophagosome closure Autophagy Breast cancer CHMP2B Cytoplasmic YAP1 EGCG ESCRT-III Hippo pathway NEDD4L Ubiquitin VPS4B

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ⅢA-ⅡB multicomponent perovskite rare earth ferrites with promising electromagnetic wave absorption properties

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Journal of Rare Earths 2024年第6期42卷 1118-1127,I0005页

作者： Yanyan He Tao chen Yanyu Pan Minzhong Huang Kaixian Wang Liyan Xue Yazhu Li fengzhi Tan Fan Yang Tongkuan Xu Heng chen School of Light Industry and Chemical Engineering Dalian Polytechnic UniversityDalian116034China Xiamen Institute of Rare EarthMaterials Haixi InstitutesChinese Academy of SciencesXiamen36021China Fujian Institute of Research on the Structure of Matter Chinese Academyof SciencesFuzhou350002China Fujian Science&Technology Innovation Laboratory for Optoelectronic Information of China Fuzhou350108China Xiamen Key-Laboratoryof Rare Earth Photoelectric Functional Materials Xiamen Institute of Rare Earth MaterialsHaixi InstituteChinese Academy of SciencesXiamen361021China Key Laboratory of Rare Earths Ganjiang Innovation AcademyChinese Academy of SciencesGanzhou3410ooChina

Perovskite-type rare-earth ferrites(REFeO_(3))are promising materials for absorbing electromagnetic(EM)wave ***,insufficient dielectric loss and poor impedance matching are key factors that limit the broader implementation of REFeO_(3).Herein,a series of multicomponent perovskite-type ferrites with strong EM wave absorption capabilities was *** the synergistic effect of chemical constitution regulation and entropy regulation,optimization of the dielectric loss and impedance matching is achieved by strengthening the structural defect mechanism,thus further adjusting the EM wave absorption *** with(LaGdSmNdBa)FeO_(3)(HE-1)and(LaGdPrSmNdBa)FeO_(3)(HE-2),(LaGdBa)FeO_(3)(ME-1)and(LaGdSmBa)FeO_(3)(ME-2)exhibit favorable performance,with optimal minimum reflection loss(RL_(min))of-56.35 dB(at 11.12 GHz)and-63.25 dB(at 7.22 GHz)and effective absorption bandwidth(EAB)of 4.46 and 4.72 GHz,*** multicomponent design provides a new strategy for the development of EM wave absorption materials.

关键词： Rare earths Multicomponent design Rare earth ferrites Entropy regulation Electromagnetic wave absorption

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Design,synthesis and biological evaluation of fluorescent derivatives of ursolic acid in living cells

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Chinese Chemical Letters 2024年第5期35卷 287-289页

作者： Wenyi Mei Lijuan Xie Xiaodong Zhang Cunjian Shi fengzhi Wang Qiqi Fu Zhenjiang Zhao Honglin Li Yufang Xu Zhuo chen Shanghai Key Laboratory of New Drug Design State Key Laboratory of Bioreactor EngineeringSchool of PharmacyEast China University of Science and TechnologyShanghai 200237China Innovation Center for AI and Drug Discovery East China Normal UniversityShanghai 200062China Lingang Laboratory Shanghai 200031China

Ursolic acid(UA)is a naturally occurring ursane triterpenoid,which exhibits a wide range of unique biological *** clarify its mechanism of action(MOA),a series of fluorescent derivatives of UA(5a-c)were designed and synthesized by conjugation with 7-nitrobenzo-2-oxa-1,3-diazole(NBD)*** them,5c exhibited similar anti-proliferative activity with UA against HCT116 cells(half maximal inhibitory concentration(IC_(50))=9.21±0.50μmol/L).Cell imaging experiment indicated that 5c was rapidly taken up in HCT116 cells in a dose and time-dependent ***,5c was found to localize in endoplasmic reticulum(ER),lysosomes,and mitochondria,but not in nucleus of HCT116 cells by confocal microscopy *** MOA proved that UA induced autophagy with a unique intracellular distribution mechanism involving ER and *** all,our work provides new clues for revealing the molecular mechanism of UA as an antitumor agent.

关键词： Pentacyclic triterpenoid Ursolic acid Fluorescent derivatives Autophagy Apoptosis

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Review on Autotoxicity of Watermelon

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Journal of Northeast Agricultural University(English Edition) 2010年第3期17卷 53-61页

作者： WU fengzhi chen Yajun ZHOU Xingang College of Horticulture Northeast Agricultural University Harbin 150030 China

As a special form of allelopathy, autotoxicity is common in cucurbits, and it is one of main factors inducing： continuous cropping obstacles. Therefore, the autotoxicity research has both theoretical and practical significance on overcoming continuous cropping obstacles. In this review, commencing on the concept of autotoxicity, research results of autotoxicity in watermelon in recent years were summarized. The significance of researches on autotoxicity in watermelon, watermelon root exudate and its autotoxicity, and the autotoxicity of watermelon stubble and the mechanisms were explained. Meanwhile, some questions needed to be further studied.

关键词： watermelon root exudate stubble autotoxicity

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临床路径在无创正压通气治疗AECOPD患者中对肺功能、睡眠质量的影响

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世界睡眠医学杂志 2022年第3期9卷 539-541,544页

作者：陈凤枝台山市中医院台山529200

目的:探究临床路径在无创正压通气治疗急性加重期慢性阻塞性肺疾病(AECOPD)患者中对肺功能、睡眠质量的影响。方法:选取2019年10月至2021年2月台山市中医院收治的AECOPD患者76例作为研究对象,按照随机数字表法分为对照组和观察组,每组3... 详细信息

目的:探究临床路径在无创正压通气治疗急性加重期慢性阻塞性肺疾病(AECOPD)患者中对肺功能、睡眠质量的影响。方法:选取2019年10月至2021年2月台山市中医院收治的AECOPD患者76例作为研究对象,按照随机数字表法分为对照组和观察组,每组38例。2组均行无创正压通气治疗,对照组为常规干预,观察组为临床路径化干预,比较2组干预前后肺功能指标(FEV1、FEV1/FVC)、匹兹堡睡眠质量指数(PSQI)评分、血气指标、并发症发生率。结果:干预后观察组肺功能指标FEV1、FEV1/FVC水平高于对照组,差异有统计学意义(P<0.05);干预后观察组PSQI评分低于对照组,差异有统计学意义(P<0.05);观察组干预后血氧饱和度(SpO_(2))及动脉血氧分压(PaO_(2))高于对照组,差异有统计学意义(P<0.05),而动脉二氧化碳分压(PaCO_(2))低于对照组,差异有统计学意义(P<0.05);观察组并发症发生率10.53%低于对照组34.21%,差异有统计学意义(P<0.05)。结论:在无创正压通气治疗AECOPD患者期间实施临床路径化干预,可显著改善肺功能与睡眠质量,利于改善患者血气指标,降低并发症发生率。

关键词：临床路径无创正压通气急性加重期慢性阻塞性肺疾病肺功能睡眠质量

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基于高效液相色谱-串联质谱测定孕妇血清中7种全氟和多氟化合物及新生儿神经行为评价

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色谱 2024年第2期42卷 194-202页

作者：王梓皓梁凤至陈学蓉吴萍吴微湖北中医药大学检验学院湖北武汉430065

以同位素为内标,建立了高效液相色谱-串联质谱测定孕妇血清中7种全氟和多氟化合物(PFASs)的方法,同时评估新生儿的神经发育水平,探讨PFASs对新生儿神经行为发育的影响。本研究依托鄂州市妇幼保健院建立的出生队列,采集孕妇孕晚期血清样... 详细信息

以同位素为内标,建立了高效液相色谱-串联质谱测定孕妇血清中7种全氟和多氟化合物(PFASs)的方法,同时评估新生儿的神经发育水平,探讨PFASs对新生儿神经行为发育的影响。本研究依托鄂州市妇幼保健院建立的出生队列,采集孕妇孕晚期血清样本,并放置于-80℃冰箱后统一检测。首先将血清样品解冻,之后依次加入13C标记的内标物、碳酸钠-碳酸氢钠缓冲液和四丁基硫酸氢铵溶液(TBAS),在超声处理后加入甲基叔丁基醚(MTBE)洗脱两次,收集所有洗脱液,涡旋混匀后离心,转移上清液至进样瓶中,随后进行检测。使用新生儿神经行为测定法(neonatal behavioral neurological assessment,NBNA)对新生儿的神经行为进行评分,评估孕期PFASs暴露对新生儿神经行为发育的影响。结果显示,7种PFASs在0.1~200 ng/mL内线性关系良好,检出限(LOD)为0.006~0.020 ng/mL,定量限(LOQ)为0.020~0.066 ng/mL,加标回收率为84.6%~116.8%。该方法所需样本量少,具有较高的准确度和精密度。实际样品检测发现,456名孕妇血清中7种PFASs的检出率均≥97.4%,其中暴露水平最高的是全氟辛酸(PFOA),中位数水平为19.4 ng/mL。同时,对379名新生儿的神经行为进行评估,线性回归模型表明,孕期母体体内的全氟辛烷磺酸(PFOS)每增加一个自然对数(ln)水平,新生儿的主动肌张力及一般反应分别降低0.36分(95%置信区间(CI):-0.64~-0.08)和0.34分(95%CI:-0.61~-0.07);全氟壬酸(PFNA)每增加一个ln水平,新生儿的被动肌张力和NBNA总分分别降低0.38分(95%CI:-0.74~-0.01)和0.37分(95%CI:-0.68~-0.06);全氟癸酸(PFDA)每增加一个ln水平,新生儿的行为能力降低0.28分(95%CI:-0.54~-0.01);而全氟己烷磺酸(PFHxS)每增加一个ln水平,新生儿的NBNA总分上升0.27分(95%CI:0.05~0.48)。性别分层分析发现,男性新生儿对PFASs的神经毒性作用更加敏感。通过此方法测定孕妇血清中的7种PFASs,并结合新生儿的神经行为评估发现,孕期PFASs暴露会影响新生儿的神经行为发育。

关键词：高效液相色谱-串联质谱全氟和多氟化合物血清孕期暴露神经行为发育

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山东省31所三级综合医院ICU护士腹内压监测行为及认知的调查研究

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中华护理杂志 2023年第21期58卷 2610-2616页

作者：刘备备李媛媛张继承杨洪娜陈凤致白雪山东第一医科大学附属省立医院重症医学科济南市250021

目的了解山东省三级综合医院ICU护士腹内压监测行为与认知的现状,并分析影响其认知的因素,为制订腹内压监测标准及流程、开展针对性培训提供参考。方法制作腹内压监测行为及认知问卷,采用便利抽样法,于2022年9月28日—11月10日从山东省1... 详细信息

目的了解山东省三级综合医院ICU护士腹内压监测行为与认知的现状,并分析影响其认知的因素,为制订腹内压监测标准及流程、开展针对性培训提供参考。方法制作腹内压监测行为及认知问卷,采用便利抽样法,于2022年9月28日—11月10日从山东省16个地级市中选择三级综合医院的ICU护士进行调查。结果共纳入31所三级综合医院(三级甲等医院24所,77.42%;三级乙等医院7所,22.58%),回收有效问卷627份。腹内压监测技术的开展率不高(73.37%),主要原因为未接受过腹内压监测相关培训(61.08%);仅111名(24.94%)护士选择了腋中线与髂嵴之间的交叉处作为测量零点、274名(61.57%)护士在呼气末进行读数。护士腹内压监测认知得分为0~16(7.88±2.79)分,回答正确率≥50%的题目仅有5题。结论山东省三级综合医院ICU护士的腹内压监测行为及认知有待加强,建议统一和落实腹内压监测标准与流程,进一步加强腹内压监测技术的培训,提高ICU护士腹内压监测的规范性和认知水平。

关键词：重症监护室护士腹内压监测认知三级医院

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上覆坚硬厚顶板煤岩卸压增透数值模拟及应用研究

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矿业安全与环保 2024年第2期51卷 67-73页

作者：袁艳梅乔伟欧聪武丰智陈龙勋永城职业学院河南永城476600 中煤科工集团重庆研究院有限公司重庆400039 河南理工大学能源科学与工程学院河南焦作454000 中国矿业大学矿业工程学院江苏徐州221116 夏阔坦矿业开发有限责任公司新疆库车842000

基于榆树田煤矿上保护层开采时被保护煤层上覆岩层为坚硬厚岩层的实际情况,采用物理实验、数值模拟和现场应用等方法,研究上保护层开采推进时被保护区域的应力变化尤其是垂直应力演化规律和原岩应力区、应力增高区和降低区的演化规律,... 详细信息

基于榆树田煤矿上保护层开采时被保护煤层上覆岩层为坚硬厚岩层的实际情况,采用物理实验、数值模拟和现场应用等方法,研究上保护层开采推进时被保护区域的应力变化尤其是垂直应力演化规律和原岩应力区、应力增高区和降低区的演化规律,继而设计保护层开采方案并选定高位定向长钻孔进行瓦斯抽采。结果表明,被保护层上覆的坚硬厚岩层会形成关键层直接影响保护层效果;上保护层开采后被保护层的卸压程度为50%,膨胀变形量为0.391%,被保护层工作面回风瓦斯浓度降低至0.35%。现场应用结果表明,被保护煤层上覆坚硬厚岩层时,采用上保护层开采配合高位定向长钻孔抽采能够取得理想的卸压增透和瓦斯抽采效果。

关键词：坚硬厚顶板卸压增透上保护层开采数值模拟瓦斯抽采

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