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Microfluidic approaches for synthetic gene circuits' construction and analysis

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Quantitative Biology 2021年第1期9卷 47-60页

作者： fengyu zhang Yanhong Sun Chunxiong Luo The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics School of PhysicsPeking UniversityBeijing 100871China School of Life Sciences and Peking-Tsinghua Center for Life Sciences Peking UniversityBeijing 100871China Center for Quantitative Biology Academy for Advanced Interdisciplinary StudiesPeking UniversityBeijing 100871China Wenzhou Institute University of Chinese Academy of Sciences Wenzhou 325001China

Background:Microfluidic systems have advantages such as a high throughput,small reaction volume,and precise control of the cellular position and *** advantages have allowed microfluidics to be widely used in several fields of synthetic biology in recent ***'.In this article,we reviewed the microfluidic-based methods for synthetic biology from two aspects:the construction of synthetic gene circuits and the analysis of synthetic gene *** used some examples to illuminate the progresses and challenges in the steps of synthetic gene circuits construction and approaches of gene expression analysis with microfluidic ***:Comparing to traditional methods,microfluidic tools promise great advantages in the synthetic genetic circuit building and analysis ***,new microfluidic systems together with the mathematical modeling of synthetic circuits or consortiums are desirable to perform complex genetic circuit construction and understand the natural gene regulation in cells and population interactions better.

关键词： microfluidics synthetic gene circuit analysis

Enhanced electrochemical performance of nanoscale single crystal NMC811 modification by coating LiNbO_(3)

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Chinese Chemical Letters 2024年第5期35卷 514-518页

作者： fengyu zhang Yali Liang zhangran Ye Lei Deng Yunna Guo Ping Qiu Peng Jia Qiaobao zhang Liqiang zhang Clean Nano Energy Center State Key Laboratory of Metastable Materials Science and Technology Yanshan UniversityQinhuangdao 066004China School of New Energy and Materials China University of Petroleum BeijingBeijing 102249China Hebei Key Laboratory of Applied Chemistry College of Environmental and Chemical EngineeringYanshan UniversityQinhuangdao 066004China State Key Laboratory of Physical Chemistry of Solid Surfaces College of MaterialsXiamen UniversityXiamen 361005China

Single crystallization is an important strategy to resolve intergranular cracks and unnecessary side reactions with electrolytes in layered transition metal oxide cathodes LiNi_(0.8)Mn_(0.1)Co_(0.1)O_(2)(NMC811).Due to the limitations of high-temperature sintering and multi-step calcination,single crystal NMC811 generally shows irregular particles with a size of 2-3μ***,the prolonged Li-ion diffusion pathway and the stress generated by the uneven de-/intercalation sluggish Li-ion diffusion kinetics,what is more,cause structural damage such as intragranular cracks.A slow Li extraction rate or particle size reduction will ameliorate the structural damage and improve the cycling *** the most promising cathodes for next-generation power batteries,NMC811 required fast charge performance and cycle *** size reduction appears to be the displacement *** is an effective strategy to mitigate intragranular cracks of single crystal ***,the serious aggregation and increased specific surface area become new *** this article,we synthesized monodisperse nanoscale single crystal NMC811 by molten salt method and modified the surface by LiNbO3 *** electrochemical performance shows that nanoscale single crystal NMC811 has faster kinetic and higher capacity retention,so the strategy of combining nanonization and surface coating is an alternative way to prepare high specific capacity and cycle stable single crystal NMC811.

关键词： Ni-rich NMC811 Molten salt method Nanonization Surface coating Micron cracks

Multiscale strain alleviation of Ni-rich cathode guided by in situ environmental transmission electron microscopy during the solid-state synthesis

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Journal of Energy Chemistry 2023年第9期84卷 467-475页

作者： fengyu zhang Yunna Guo Chenxi Li Tiening Tan Xuedong zhang Jun Zhao Ping Qiu Hongbing zhang Zhaoyu Rong Dingding Zhu Lei Deng zhangran Ye Zhixuan Yu Peng Jia Xiang Liu Jianyu Huang Liqiang zhang School of New Energy and Materials China University of Petroleum BeijingBeijing 102249China Clean Nano Energy Center State Key Laboratory of Metastable Materials Science and Technology Yanshan UniversityQinhuangdao 066004HebeiChina School of Materials Science and Engineering Beihang UniversityBeijing 100191China Sichuan New Energy Vehicle Innovation Center Yibin 644000SichuanChina School of Materials Science and Engineering Xiangtan UniversityXiangtan 411105HunanChina Peking University Institute of Advanced Agricultural Sciences Shandong Laboratory of Advanced Agricultural Sciences at WeifangWeifang 261000ShandongChina China Oil&Gas Pipeline Network Corporation Department of Engineering Beijing 100026China Hebei Key Laboratory of Applied Chemistry College of Environmental and Chemical EngineeringYanshan UniversityQinhuangdao 066004HebeiChina Key Laboratory of Low Dimensional Materials and Application Technology of Ministry of Education School of Materials Science and EngineeringXiangtan UniversityXiangtan 411105HunanChina

Ni-rich layered oxides are one of the most promising cathode materials for Li-ion batteries due to their high energy ***,the chemomechanical breakdown and capacity degradation associated with the anisotropic lattice evolution during lithiation/delithiation hinders its practical ***,by utilizing the in situ environmental transmission electron microscopy(ETEM),we provide a real time nanoscale characterization of high temperature solid-state synthesis of LiNi_(0.8)CO_(0.1)Mn_(0.1)O_(2)(NCM811) cathode,and unprecedentedly reveal the strain/stress formation and morphological evolution mechanism of primary/second ary particles,as well as their influence on electrochemical *** show that stress inhomogeneity during solid-state synthesis will lead to both primary/secondary particle pulverization and new grain boundary initiation,which are detrimental to cathode cycling stability and rate *** to alleviate this multiscale strain during solid-state synthesis,we introduced a calcination scheme that effectively relieves the stress during the synthesis,thus mitigating the primary/secondary particle crack and the detrimental grain boundaries formation,which in turn improves the cathode structural integrity and Li-ion transport kinetics for long-life and high-rate electrochemical *** work remarkably advances the fundamental understanding on mechanochemical properties of transition metal oxide cathode with solid-state synthesis and provides a unified guide for optimization the Ni-rich oxide cathode.

关键词： Ni-rich cathode In situ ETEM Solid-state synthesis Multiscale strain alleviation

Design of Human Resource Performance Evaluation System for Construction Enterprises

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International English Education Research 2014年第3期 1-4页

作者： WANG Zhineng zhang fengyu College of Economics and Management China Three Gorges University Yichang Hubei China 443002

Based on job analysis for the permanent departments of construction enterprises, by combining KPI assessment method and 360-degree evaluation method, this article establishes a set of human resource performance evaluation indicator system; by determining indicators＇ weights and evaluators＇ grading weights through analytic hierarchy process （AHP）, offering a kind of dynamic computing method for performance evaluation, and tightly linking the evaluation result to employees＇ personal interests, this article builds up a set of scientific performance evaluation system. This article takes the engineering department of construction enterprises for example to explain the calculation and application of performance evaluation result. This article provides construction enterprises with a method reference for human resource performance evaluation.

关键词： Construction enterprises Human resources Performance evaluation KPI assessment

Whole-genome sequencing of monozygotic twins discordant for schizophrenia indicates multiple genetic risk factors for schizophrenia

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Journal of Genetics and Genomics 2017年第6期44卷 295-306页

作者： Jinsong Tang Yu Fan Hong Li Qun Xiang Deng-Feng zhang Zongchang Li Ying He Yanhui Liao Ya Wang Fan He fengyu zhang Yin Yao Shugart Chunyu Liu Yanqing Tang Raymond C.K.Chan Chuan-Yue Wang Yong-Gang Yao Xiaogang Chen Institute of Mental Health National Clinical Research Center for Mental Health Disorders and National Technology Institute of Psychiatryand Key Laboratory of Psychiatry and Mental Health of Hunan ProvinceThe Second Xiangya HospitalCentral South University Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province Kunming Institute of Zoology Department of Psychiatry The First Affiliated Hospital of Zhengzhou University Kunming College of Life Science University of Chinese Academy of Sciences Neuropsychology and Applied Cognitive Neuroscience Laboratory and CAS Key Laboratory of Mental HealthInstitute of PsychologyChinese Academy of Sciences Beijing Key Laboratory of Mental Disorders Department of PsychiatryBeijing Anding Hospitaland Center of SchizophreniaBeijing Institute for Brain Disorders and Laboratory of Brain Disorders of the Ministry of Science and TechnologyCapital Medical University Unit on Statistical Genomics Intramural Research ProgramsNational Institute of Mental HealthNIH Institute of Human Genetics University of Illinois at Chicago Department of Psychiatry The First Affiliated Hospital of China Medical University CAS Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of Sciences

Schizophrenia is a common disorder with a high heritability, but its genetic architecture is still *** implemented whole-genome sequencing(WGS) analysis of 8 families with monozygotic(MZ) twin pairs discordant for schizophrenia to assess potential association of de novo mutations(DNMs) or inherited variants with susceptibility to schizophrenia. Eight non-synonymous DNMs(including one splicing site) were identified and shared by twins, which were either located in previously reported schizophrenia risk genes(p.V24689 I mutation in TTN, p.S2506 T mutation in GCN1L1, IVS3+1G > T in DOCK1) or had a benign to damaging effect according to in silico prediction analysis. By searching the inherited rare damaging or loss-of-function(LOF) variants and common susceptible alleles from three classes of schizophrenia candidate genes, we were able to distill genetic alterations in several schizophrenia risk genes, including GAD1, PLXNA2, RELN and FEZ1. Four inherited copy number variations(CNVs; including a large deletion at 16p13.11) implicated for schizophrenia were identified in four families, respectively. Most of families carried both missense DNMs and inherited risk variants, which might suggest that DNMs, inherited rare damaging variants and common risk alleles together conferred to schizophrenia susceptibility. Our results support that schizophrenia is caused by a combination of multiple genetic factors, with each DNM/variant showing a relatively small effect size.

关键词： Whole-genome sequencing Schizophrenia Monozygotic twin De novo mutation Combined effect Susceptibility

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论第二轮县志艺文入志问题

中国地方志 2007年第12期 19-21页

作者：张凤雨河北省武强县地方志办公室 053300

旧志艺文占据比重甚大,社会主义首轮志书艺文内容又过少。第二轮县志如何处理艺文内容入志是一个需认真考虑的问题。本文从新编县级志书艺文部分应包含哪些内容,旧志与首轮县志艺文入志情况的比较,第二轮县志艺文志内容选择分析、写法... 详细信息

旧志艺文占据比重甚大,社会主义首轮志书艺文内容又过少。第二轮县志如何处理艺文内容入志是一个需认真考虑的问题。本文从新编县级志书艺文部分应包含哪些内容,旧志与首轮县志艺文入志情况的比较,第二轮县志艺文志内容选择分析、写法等方面进行了探讨。

关键词：艺文入志探讨

TAK-242 affects Wallerian degeneration and peripheral nerve regeneration by regulating innate immunity in rats

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TAK-242 affects Wallerian degeneration and peripheral nerve ...

直流微电网基于等价输入干扰的自适应无源控制

第十一届全国免疫学学术大会

作者： Xiong Le zhang Li Ren Shureng zhang fengyu Wang Yi zhang Bei Medical College of Qingdao University

Toll-like receptor 4(TLR4) recognize PAMPs and DAMPs during innate immunity. Wallerian degeneration(WD) is a process of immune response following peripheral nerve injury. In order to explore the role of TLR4 signal pathway during Wallerian degeneration, TAK-242, a small molecular TLR4 signaling inhibitor, was administrated to rats intravenously 1 h before nerve injury. Subsequent injections of TAK-242 were given daily for 7 consecutive days. Here, we investigated whether TLR4 signaling is involved in WD and nerve regeneration after peripheral nerve injury. Using realtime quantitative RT-PCR(q RT-PCR) and immunohistochemistry(IHC), we found that the expression of TIR-domaincontaining adaptor inducinginterferon-β(TRIF), interleukin-1(IL-1β) and monocyte chemoattractant protein-1(MCP-1) was compromised in the sciatic nerve in TAK-242-injected rats compared with controls. Immunofluorescence(IF) staining studies showed that microglia and macrophages are recruited in the sciatic nerve distal stump following peripheral nerve injury, and they were decreased significantly in TAK-242-injected rats at 3 d post-injury compared with controls. Luxol Fast Blue(LFB) staining were used to test sciatic nerves myelin clearance at 7 d post-injury, and the data showed that compared with controls, myelin debris clearance in TAK-242-injected rats significantly delayed. In addition, we use growth associated protein-43(GAP-43) immunohistochemistry to assessed axonal regeneration at 4 d post-injury, and the data showed a delay on axonal regeneration in TAK-242-injected rats compared with saline-injected rats. Haematoxylineosin(HE) staining also indicated a poor organization of repair site in TAK-242-injected rats in distal stump at 7 d postinjury. In addition, Finally, SFI of TAK-242-injected rats were decreased significantly at 20, 30 and 40 d after injury compared with that of controls respectively. These results indicate that TLR4 signaling is possible to manipulate the innate immune resp

关键词： TAK-242 toll-like receptor 4 Wallerian degeneration peripheral nerve

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高电压技术 2019年第1期45卷 236-242页

作者：杨帆张峰毓李东东上海电力学院电气工程学院上海200090

针对直流微电网孤岛运行时,线路阻抗的不确定性和故障等作用致使控制器输出受系统参数突变的问题,提出了一种基于等价输入干扰的自适应无源控制方法(EID-APBC)。首先采用强跟踪滤波器在线估计出由不确定系统参数变化所引起的控制器等价... 详细信息

针对直流微电网孤岛运行时,线路阻抗的不确定性和故障等作用致使控制器输出受系统参数突变的问题,提出了一种基于等价输入干扰的自适应无源控制方法(EID-APBC)。首先采用强跟踪滤波器在线估计出由不确定系统参数变化所引起的控制器等价输入干扰,然后由前馈补偿器对控制器的控制量进行修正补偿,最终消除直流微电网稳定域内的稳态控制误差。仿真结果表明,当系统参数发生变化时,直流微电网母线电压仍能保持在期望的参考值。EID-APBC控制的直流微电网在系统稳定域内具有良好的鲁棒性和控制性能。

关键词：直流微电网强跟踪滤波器等价输入干扰自适应无源控制鲁棒性抗干扰性

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数字时代的算法滥用及其规制研究

经济学动态 2023年第2期 71-87页

作者：张丰羽汤珂清华大学社会科学学院经济学研究所 100084

在数字经济时代,算法技术结合大数据和机器学习后已被应用于自动化或辅助决策、精准匹配和趋势预测等诸多生产或管理活动中。伴随其在商业和公共管理等领域的广泛深刻运用,因算法权利异化导致的算法滥用问题逐渐受到人们的担忧和重视。... 详细信息

在数字经济时代,算法技术结合大数据和机器学习后已被应用于自动化或辅助决策、精准匹配和趋势预测等诸多生产或管理活动中。伴随其在商业和公共管理等领域的广泛深刻运用,因算法权利异化导致的算法滥用问题逐渐受到人们的担忧和重视。算法滥用存在针对不同主体的诸多现实或潜在的危害与弊端,足以影响到数字经济的健康可持续发展。因此,数字经济时代引致出针对算法进行规制和监管的需求。不同于传统的规制和监管的范式与手段,针对算法进行规制监管存在诸多问题与挑战。通过分析当前典型的算法规制思路与治理范式,结合算法规制监管的实施特点和痛点难点,本文针对性地提出建立以广义算法公开为基础的分场景算法审查、监管和评议的综合技术规制框架;建立在算法伦理基础上的外部监管问责、平台责任义务相结合的法规体系;建立以个体数据赋权为主要形式的健全维权途径、强化维权意识和配置个人算法权利的数据主体算法权利保障;建立算法设计主体价值与算法相关主体价值收敛协同发展、强化算法设计者社会责任的具体实现路径,以图探求适合中国数字经济背景下的算法规制体系建设思路。

关键词：数字经济算法滥用算法规制算法监管