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A Critical Role of AMT2;1 in Root-To-Shoot Translocation of Ammonium in Arabidopsis

Molecular Plant 2017年第11期10卷 1449-1460页

作者： Ricardo F.H. Giehl Alberto M. Laginha fengying duan Doris Rentsch Lixing Yuan Nicolaus von Wirén Department of Physiology and Cell Biology Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Corrensstrasse 3 06466 Gatersleben Germany Institute of Plant Sciences University of Bern Aitenbergrain 21 3013 Bern Switzerland Key Lab of Plant-Soil Interaction MOE College of Resources and Environmental Sciences China Agricultural University Beijing 100193 China

Ammonium uptake in plant roots is mediated by AMT/MEP/Rh-type ammonium transporters. Out of five AMTs being expressed in Arabidopsis roots, four AMT1-type transporters contribute to ammonium uptake, whereas no physiological function has so far been assigned to the only homolog belonging to the MEP subfamily, AMT2;1. Based on the observation that under ammonium supply, the transcript levels of AMT2;1 increased and its promoter activity shifted preferentially to the pericycle, we assessed the contribution of AMT2;1 to xylem loading. When exposed to ^15N-labeled ammonium, amt2;1 mutant lines translocated less tracer to the shoots and contained less ammonium in the xylem sap. Moreover, in an amtl;1 amtl;2 amtl ;3 amt2;1 quadruple mutant （qko）, co-expression of AMT2;1 with either AMT1;2 or AMT1;3 significantly enhanced ^15N translocation to shoots, indicating a cooperative action between AMT2;1 and AMT1 transporters. Under N deficiency, proAMT2;1-GFP lines showed enhanced promoter activity predominantly in cortical root cells, which coincided with elevated ammonium influx conferred by AMT2;1 at millimolar sub- strate concentrations. Our results indicate that in addition to contributing moderately to root uptake in the low-affinity range, AMT2;1 functions mainly in root-to-shoot translocation of ammonium, depending on its Cell-type-specific expression in response to the plant nutritional status and to local ammonium gradients.

关键词： nitrogen uptake nitrogen translocation ammonium assimilation xylem loading ammonia transport ammonium influx

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在矛盾的处理和解决中构建和谐社会

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中共山西省直机关党校学报 2008年第3期 26-27页

作者：段凤英山西省第三建筑工程公司山西长治046011

我们取得了举世瞩目的成就,但也应正确认识所遇到的困难和问题。客观分析我们所面临的社会矛盾,并提出解决的途径,是执政党的使命所在。要解决前进中的困难和问题关键在于我们党不断加强和改进自身建设,在正确处理人民内部矛盾中构建社... 详细信息

我们取得了举世瞩目的成就,但也应正确认识所遇到的困难和问题。客观分析我们所面临的社会矛盾,并提出解决的途径,是执政党的使命所在。要解决前进中的困难和问题关键在于我们党不断加强和改进自身建设,在正确处理人民内部矛盾中构建社会主义和谐社会。

关键词：和谐社会矛盾问题党的建设

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A reference-guided TILLING by amplicon-sequencing platform supports forward and reverse genetics in barley

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Plant Communications 2022年第4期3卷 37-53页

作者： Congcong Jiang Miaomiao Lei Yu Guo Guangqi Gao Lijie Shi Yanlong Jin Yu Cai Axel Himmelbach Shenghui Zhou Qiang He Xuefeng Yao Jinhong Kan Georg Haberer fengying duan Lihui Li Jun Liu Jing Zhang Manuel Spannagl Chunming Liu Nils Stein Zongyun Feng Martin Mascher Ping Yang Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijingChina College of Agronomy Sichuan Agricultural UniversityChengduChina Leibniz Institute of Plant Genetics and Crop Plant Research(IPK) SeelandGermany Institute of Botany Chinese Academy of SciencesBeijingChina Plant Genome and Systems Biology(PGSB) Helmholtz Center MunichGerman Research Center for Environmental HealthNeuherbergGermany

Barley is a diploid species with a genome smaller than those of other members of the Triticeae tribe,making it an attractive model for genetic studies in Triticeae *** recent development of barley genomics has created a need for a high-throughput platform to identify genetically uniform mutants for gene function *** this study,we report an ethyl methanesulfonate(EMS)-mutagenized population consisting of 8525M_(3) lines in the barley landrace“Hatiexi”(HTX),which we complement with a high-quality de novo assembly of a reference genome for this *** mutation rate within the population ranged from 1.51 to 4.09 mutations per megabase,depending on the treatment dosage of EMS and the mutation discrimination platform used for genotype *** implemented a three-dimensional DNA pooling strategy combined with multiplexed amplicon sequencing to create a highly efficient and cost-effective TILLING(targeting induced locus lesion in genomes)platform in *** were successfully identified from 72 mixed amplicons within a DNA pool containing 64 individual mutants and from 56 mixed amplicons within a pool containing 144 *** discovered abundant allelic mutants for dozens of genes,including the barley Green Revolution contributor gene Brassinosteroid insensitive 1(BRI1).As a proof of concept,we rapidly determined the causal gene responsible for a chlorotic mutant by following the MutMap strategy,demonstrating the value of this resource to support forward and reverse genetic studies in barley.

关键词： barley mutagenesis TILLING amplicon-seq genetics

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Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： fengying Sun Haoyan Li Dongqing Sun Shaliu Fu Lei Gu Xin Shao Qinqin Wang Xin Dong Bin duan Feiyang Xing Jun Wu Minmin Xiao Fangqing Zhao Jing-Dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

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EGFR甲基化对肺腺癌细胞裸鼠移植瘤生长的影响

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实验与检验医学 2023年第5期41卷 540-542,553页

作者：郭玲玲段凤英江西省人民医院呼吸与危重医学科南昌大学第二附属医院呼吸与危重症医学科江西南昌330006

目的探讨EGFR启动子甲基化对肺腺癌细胞裸鼠移植瘤生长的影响,为解决肺腺癌细胞对EGFR-TKIs耐药提供新的方法。方法采用甲基化转移酶抑制剂5-氮杂-2’-脱氧胞苷(5-aza-CdR)及吉非替尼(一代EGFR-TKI),分别及联合处理肺腺癌细胞株H1299... 详细信息

目的探讨EGFR启动子甲基化对肺腺癌细胞裸鼠移植瘤生长的影响,为解决肺腺癌细胞对EGFR-TKIs耐药提供新的方法。方法采用甲基化转移酶抑制剂5-氮杂-2’-脱氧胞苷(5-aza-CdR)及吉非替尼(一代EGFR-TKI),分别及联合处理肺腺癌细胞株H1299(原发耐药)、H1975(T790M突变,继发耐药)、HCC827(19外显子敏感突变)。进一步将三株细胞建成裸鼠移植瘤模型,观察在裸鼠体内5-aza-CdR是否可以提高肺腺癌细胞对吉非替尼的敏感性。结果通过裸鼠移植瘤试验证实在活体内5-aza-CdR联合吉非替尼可以提高继发性耐药细胞株H1975对吉非替尼的敏感性。结论 EGFR启动子高甲基化可能参与了肺腺癌细胞对吉非替尼的继发性耐药。5-aza-CdR联合吉非替尼可以部分改善肺腺癌细胞对吉非替尼的继发性耐药。

关键词：肺腺癌表皮生长因子酪氨酸激酶抑制剂吉非替尼甲基化裸鼠

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ICU拔除气管插管患者使用经鼻高流量湿化氧疗对比传统氧疗或无创正压通气临床疗效的Meta分析

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中国呼吸与危重监护杂志 2019年第1期18卷 42-50页

作者：简佳庆段凤英肖远超张思颖高飞南昌大学第二附属医院呼吸内科江西南昌330006

目的系统评价重症加强治疗病房(ICU)患者拔除气管插管后使用经鼻高流量氧疗(HFNC)的临床有效性。方法检索PubMed、Embase、Cochrane图书馆、中国知网、万方、维普等数据库关于HFNC对拔除气管插管疗效的随机对照临床试验(RCT)或队列研究... 详细信息

目的系统评价重症加强治疗病房(ICU)患者拔除气管插管后使用经鼻高流量氧疗(HFNC)的临床有效性。方法检索PubMed、Embase、Cochrane图书馆、中国知网、万方、维普等数据库关于HFNC对拔除气管插管疗效的随机对照临床试验(RCT)或队列研究相关研究,试验组采用HFNC,对照组采用面罩或鼻导管给氧方式的传统氧疗(COT)或无创正压通气(NIPPV)。由2名研究员按照纳入和排除标准筛选文献,评价纳入文献的质量并提取资料,使用RevMan5.3软件进行统计分析。主要结局观察指标:气管再插管率、ICU留院天数;次要观察指标:ICU死亡率、拔管后院内肺部感染率。结果纳入20篇文献(3篇队列研究,17篇RCT研究),共3 583例研究对象,HFNC 1 727例,对照组1 856例(COT 841例、NIPPV 1 015例)。Meta分析结果显示,在降低再插管率方面,HFNC较COT有明显的优势(P<0.000 01),HFNC与NIPPV比较差异无统计学意义(P=0.21);两亚组合并分析显示:与COT/NIPPV相比,HFNC在减轻拔管后再插管率方面有明显优势(P<0.000 01);在ICU病死率方面,HFNC与COT(P=0.38)、与NIPPV(P=0.36)比较差异均无统计学意义;在ICU住院天数方面,HFNC与COT(P=0.30)比较差异无统计学意义,与NIPPV比较差异有统计学意义(P<0.000 01),两个亚组合并分析后差异有统计学意义(P=0.04);拔管后院内肺部感染率方面,HFNC与COT(P=0.61)、与NIPPV(P=0.23)比较差异均无统计学意义。针对不同研究类型,再进行亚组分析后,分析结果跟合并后无差异。结论对于拔管患者,HFNC与COT相比,可降低气管再插管率,而ICU病死率、住院天数、院内肺部感染率差异无统计学意义;HFNC与NIPPV相比,可降低住院天数,而气管再插管率、ICU病死率、院内肺部感染率差异无统计学意义。

关键词：拔管后经鼻高流量湿化氧疗再插管率 ICU住院天数 ICU病死率院内肺部感染

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节能型钢包烘烤器的研究和设计初探

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工业炉 1999年第3期21卷 40-41+46页

作者：涂卫国段凤英宣炎马钢股份公司设计院!邮编:马鞍山243000

本文概述了烘包的意义，简介了国内外烘包器的现状，以及作者设计烘包器的经验，对马钢三钢厂烘包器改造做了简要的技术经济分析，指出了现阶段对我国落后的烘包器改造的必要性及意义，并对我国烘包器的发展做了展望。

关键词： ladle preheating ladle roaster self preheating burner

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Preparation of a novel cross-linked polyetherimide with enhanced breakdown strength and high-temperature energy storage performance

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High Voltage 2023年第3期8卷 630-639页

作者： Guangyu duan fengying Hu Ruina Zhang Zuming Hu College of Materials Engineering Henan University of EngineeringZhengzhouChina Henan Engineering Technology Research Center for Fiber Preparation and Modification Henan University of EngineeringZhengzhouChina State Key Laboratory for Modification of Chemical Fibers and Polymer Materials Donghua UniversityShanghaiChina

Polymer dielectrics with excellent energy storage performance at high temperature are urgently needed in advanced applications,such as hybrid electric vehicles,smart grid and pulsed power ***(PEI),which is supposed to be the most promising candidate among polymer dielectric materials,displays a limitation for high-temperature polymer dielectrics owing to the rapidly decreasing discharged energy density(U_(d))and charge-discharge efficiency(η).Herein,a novel PEI with cross-linked networks was firstly prepared by utilising 2,4,6-Triaminopyrimidine(TAP)as a *** results showed that the breakdown strength of the cross-linked PEI(c-PEI)with 2 wt%TAP increased to 399.4 MV/m,showing an increment of 23.3%in comparison with noncross-linked ***,owing to the restrained polarisation loss and relaxation loss by cross-linked networks,the dielectric loss of c-PEI dielectric gradually decreased with the increasing content of *** particular significance was the c-PEI dielectrics exhibiting improved U_(d)andηat high *** maximum U_(d)of c-PEI with 2 wt%TAP was 2.53 J/cm3 at 150℃,which was 24.8%higher than non-cross-linked PEI(2.11 J/cm^(3)at 150℃).This research provides an innovative strategy to achieve novel PEI dielectrics with improved U_(d)andηat high temperature.

关键词： breakdown dielectric strength

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肺黏膜相关淋巴组织淋巴瘤临床特征分析

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国际呼吸杂志 2016年第4期36卷 254-257页

作者：郭玲玲段凤英童波赖红琳吴西雅涂红缨肖祖克江西省人民医院呼吸内科南昌330006 南昌大学第二附属医院呼吸内科 330006

目的提高肺黏膜相关淋巴组织淋巴瘤诊治水平。方法回顾性分析江西省人民医院及南昌大学第二附属医院自2005年1月至2015年1月确诊的8例肺黏膜相关淋巴组织淋巴瘤的临床表现、影像学特点、诊断手段、误诊情况、治疗及预后。结果8例肺黏... 详细信息

目的提高肺黏膜相关淋巴组织淋巴瘤诊治水平。方法回顾性分析江西省人民医院及南昌大学第二附属医院自2005年1月至2015年1月确诊的8例肺黏膜相关淋巴组织淋巴瘤的临床表现、影像学特点、诊断手段、误诊情况、治疗及预后。结果8例肺黏膜相关淋巴组织淋巴瘤中男6例，女2例，年龄38～75岁，中位年龄65岁。主要症状：咳嗽（5例）、咳痰（4例）、发热（2例）、胸闷（4例）、乏力（3例）、消瘦（3例），无症状2例。胸部CT表现：双肺分布5例，单肺分布3例，实变影5例，肿块及结节影4例，斑片状浸润影3例，支气管充气征5例，钙化1例，空洞2例。确诊方法：经气管镜活检1例，CT引导下经皮肺穿刺5例，外科手术2例。误诊分析：5例误诊为细菌性肺炎，1例误诊为肺真菌病，1例误诊为肺癌，1例误诊为肺转移癌。治疗及预后：2例外科手术患者术后未行放化疗，4例转入血液内科行化疗（CHOP方案），2例放弃治疗。8例患者随访时间2～105个月，2例死亡，6例存活。结论肺黏膜相关淋巴组织淋巴瘤临床表现不典型，容易误诊，诊断需要组织病理活检。

关键词：肺黏膜相关淋巴组织淋巴瘤临床特征

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Goal directed fluid therapy with LiDCOrapid in a parturient with WPW syndrome and cardiac dysfunction undergoing emergent cesarean section

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Chinese Medical Journal 2014年第14期127卷 2719-2720页

作者： Xiao Wei duan Qingfang Zhao Lei Wang fengying Wang Tianlong Capital Med Univ Xuanwu Hosp Dept Anesthesiol Beijing 100053 Peoples R China Capital Med Univ Xuanwu Hosp Dept Obstet & Gynecol Beijing 100053 Peoples R China

To the editor： A 26-year-old parturient at 376/7 weeks＇ gestation presenting light activity induced stuffiness, palpitation and breathlessness and incapability of lying flat was urgently admitted to our hospital. Her BMI was 35 kg/m2 （height 163 cm, weight 93 kg）. Upon admission, the physical examination showed pulse rate of 100-120 beats/min, blood pressure of 140/70 mmHg. Expanded border of cardiac dullness, moist rales over the fight lung base, and mild edema in her bilateral lower extremities were exhibited. Her electrocardiograph revealed ongoing sinus tachycardia with positive delta waves in I, II, AVL, V2-V6 leads and abnormal ST segment. The echocardiogram indicated a normal ejection fraction of 53% and an enlarged left heart with atrial dimension of 37 mm and ventricular internal diastolic diameter of 54 mm. A quantitative urinary protein test showed an elevated urinary protein excretion at 0.48 g within 24 hours. The diagnosis of Wolf-Parkinson-White （WPW） syndrome can be traced back to her first pregnancy two years ago. The relevant electrophysiological examination was ignored after a smooth cesarean section. However, during the second pregnancy the symptoms of impaired cardiac function appeared after 36 weeks＇ gestation. Her cardiac function was classified as NYHA III.

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