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Clinical relevance of distolingual roots and periodontal status in mandibular first molars: a cross-sectional study employing CBCT analysis

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第3期25卷 244-253页

作者： Feifei MAO Meng WANG Shuai ZHOU Yan ZHAO Jiaping huang fengying YIN Haiping YANG Pei-hui DING Stomatology Hospital School of StomatologyZhejiang University School of MedicineZhejiang Provincial Clinical Research Center for Oral DiseasesKey Laboratory of Oral Biomedical Research of Zhejiang ProvinceCancer Center of Zhejiang UniversityHangzhou 310016China

Objectives:Distolingual root of the permanent mandibular first molar(PMFM-DLR)has been frequently reported,which may complicate the treatment of periodontitis.This study aimed to assess the morphological features of PMFM-DLR and investigate the correlation between the morphological features of PMFM-DLR and periodontal status in patients with Eastern Chinese ethnic background.Materials and methods:A total of 836 cone beam computed tomography(CBCT)images with 1497 mandibular first molars were analyzed to observe the prevalence of PMFM-DLR at the patients and tooth levels in Eastern China.Among them,complete periodontal charts were available for 69 Chinese patients with 103 teeth.Correlation and regression analyses were used to evaluate the correlation between the morphological features of DLR,bone loss,and periodontal clinical parameters,including clinical attachment loss(CAL),probing pocket depth(PPD),gingival recession(GR),and furcation involvement(FI).Results:The patient-level prevalence and tooth-level prevalence of DLR in mandibular first molars were 29.4%and 26.3%,respectively.Multiple linear regression analysis suggested that bone loss at the lingual site and CAL were negatively affected by the angle of separation between distolingual and mesial roots in the transverse section,while they were significantly influenced by age and the angle of separation between distobuccal and mesial roots in the coronal section.Conclusions:The prevalence of PMFM-DLR in Eastern China was relatively high in our cohort.The morphological features of DLR were correlated with the periodontal status of mandibular first molars.This study provides critical information on the morphological features of DLR for improved diagnosis and treatment options of mandibular molars with DLR.

关键词： Distolingual root Mandibular first molar Periodontal clinical parameter Clinical attachment loss Cone beam computed tomography(CBCT)

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A novel therapeutic vaccine based on graphene oxide nanocomposite for tumor immunotherapy

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Chinese Chemical Letters 2022年第8期33卷 4089-4095页

作者： Liming Zhang Lingfeng Xu Yi Wang Jieyu Liu Guanghong Tan fengying huang Nongyue He Zhuoxuan Lu Key Laboratory of Tropical Translational Medicine of Ministry of Education&Hainan Provincial Key Laboratory of Tropical Medicine Hainan Medical UniversityHaikou 571199China State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Department of Clinical Laboratory HwaMei HospitalUniversity of Chinese Academy of SciencesNingbo 315000China

With an intensive understanding of the mechanism of immune system,developing a therapeutic tumor vaccine is one of the most perspective strategy of cancer immunotherapy.In this study,we report a facile approach to prepare graphene oxide(GO)-based therapeutic cancer-nanovaccine.The model antigen(ovalbumin,OVA)and adjuvant(CpG ODN),are conjugated with GO-PEI nanosheet through electrostatic interaction.The addition of PEG can improve biocompatibility and prevent nanoparticle aggregation.The prepared GO-based nanovaccine,GO-PEI-OVA-PEG-CpG,exhibits good biocompatibility and low toxicity both in vivo and in vitro.More importantly,it can efficiently induce the maturation of dendritic cells(DCs),the enhancement of antigen cross-presentation ability,and the amplification of cytokine production of immune cells.Impressively,this nanovaccine shows a remarkable therapeutic effect against preestablished B16-OVA-melanoma tumors,which can significantly inhibit tumor growth and prolong the survival time of the OVA-expressed tumor-bearing mice.Moreover,combining GO-PEI-OVA-PEG-CpG with NLG919,an IDO-1(indoleamine-2,3-dioxygenase)inhibitor which can regulate the tumor microenvironment,displays a synergistic therapeutic effect.These findings indicate the GO-PEI-OVA-PEG-CpG nanovaccine actively induces an antigen-specific antitumor immune response and it combined with NLG919 could achieve better therapeutic outcomes.

关键词： Nanovaccine Graphene Oxide Adjuvant Tumor-specific antigen NLG919

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Isolation and identification of multidrug-resistant Staphylococcus haemolyticus from a laboratory-breeding mouse

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Asian Pacific Journal of Tropical Medicine 2011年第6期4卷 421-425页

作者： fengying huang Qiuping Meng Guanghong Tan Yonghao huang Hua Wang Wenli Mei Haofu Dai Hainan Provincial Key Laboratory of Tropical Medicine Hainan Medical College Agriculture School Hainan University Biological Technology Institute Chinese Academy of Tropical Agricultural Sciences

Objective:To analysis and identify a bacterium strain isolated from laboratory breeding mouse far away from a hospital.Methods:Phenotype of the isolate was investigated by conventional microbiological methods,including Gram-staining,colony morphology,tests for haemolysis, catalase,coagulase,and antimicrobial susceptibility test.The meek and 16S rRNA genes were amplified by the polymerase chain reaction(PCR) and sequenced.The base sequence of the PCR product was compared with known 16S rRNA gene sequences in the CenBank database by phylogenetic analysis and multiple sequence alignment.Results:The isolate in this study was a gram positive,coagulase negative,and catalase positive coccus.The isolate was resistant to oxacillin,methicillin,penicillin,ampicillin,cefazolin,cipr of loxacin erythromycin,et al.PCR results indicated that the isolate was meek gene positive and its 16S rRNA was 1465 bp.Phylogenetic analysis of the resultant 16S rRNA indicated the isolate belonged to genus Saphylococcus,and multiple sequence alignment showed that the isolate was Saphylococcus haemolyticus with only one base difference from the corresponding 16S rRNA deposited in the CenBank.Conclusions: 16S rRNA gene sequencing is a suitable technique for non-specialist researchers.Laboratory animals are possible sources of lethal pathogens,and researchers must adapt protective measures when they manipulate animals.

关键词： 16S rRNA Gene sequences analysis Staphylococcus haemolyticus Multidrug resistant

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Construction and identification of recombinant plasmid pUIS3-BLC^+

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Asian Pacific Journal of Tropical Medicine 2010年第10期3卷 762-764页

作者： Yingzi Lin Shixiang Bao Guanghong Tan fengying huang Huiqin huang Agriculture School of Hainan University Biological Technology Institute Chinese Academy of Tropical Agricultural Sciences Hainan Provincial Key Laboratory of Tropical Medicine Hainan Medical University

Objective:To construct and identify recombinant plasmid pUIS3-BLC^+.Methods:The cDNA of B-lymphocyte chemoattractant(BLC) was amplified from the total RNA of spleen tissues by PCR method,and were inserted into plasmid of Plasmodium berghei with U1S3 knockout by digestion of restrictive endonuclease and T7 ligation.The recombinant plasmids were screened,and then underwent restriction enzymatic digestion and DNA sequencing.Then the confirmed plasmid was further transfected into COS-1 cells by lipofectamine and the BLC expression was tested by RT-PCR and Western blotting.Results:The cDNA of BLC gene was correctively amplified by RT-PCR and the recombinant plasmid pUIS3-BLC^+ was constructed successfully,which was confirmed by restriction enzymatic digestion and DNA sequencing.RT-PCR and Western blotting also showed the BLC gene expression in COS-1 cells.Conclusions:The recombinant plasmid pUIS3-BLC^+ has BLC expression in COS-1 cells,and is useful for further study on BLC transgene and UIS3 gene knockout in Plasmodium berghei.

关键词： BLC Plasmodium berghei Transgene Knockout

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Cy5.5-MSA-G250 nanoparticles(CMGNPs)induce M1 polarity of RAW264. 7 macrophage cells via TLR4-dependent manner

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Chinese Chemical Letters 2019年第6期30卷 1320-1324页

作者： Zhuoxuan Lu Lingfeng Xu Nongyue He fengying huang Tiefeng Xu Li Li Yanwei Zhang Liming Zhang Key Laboratory of Tropical Disease and Translational Medicine of the Ministry of Education&Hainan Provincial Key Laboratory of Tropical Medicine Hainan Medical College Haikou 571101 China Hunan Key Laboratory of Biomedical Materials and Devices Hunan University of Technology Zhuzhou 412008 China The First Affiliated Hospital and the Oncological Institute of Hainan Medical College Haikou 571101 China

Cy5.5-MSA-G250 nanoparticles(CMGNPs)had been proved to have unique advantages for cancer treatment,including excellent photothermal performance,tumor cell-selective cytotoxicity,direct visualization,and good biocompatibility.However,to cellular systems,the CMGNPs are considered as fo reign invaders,and the effect of CMGNPs on immunity system is still unknown.Therefore,more efforts are needed to understand the role of CMGNPs on the immunity system.In this study,we attempted to screen the pro-inflammatory responses on RAW264.7 macrophages after treated with the CMGNPs.In vitro experiments clearly showed that CMGNPs not only enhances phagocytosis capacity of RAW264.7 cells,but also promotes Ml polarization,associated with changes in cell morphology and increased expression of inflammatory cytokines.This ability to induce Ml polarization may be beneficial to CMGNPs to achieve better anticancer effects in clinical trials.Moreover,the observed Ml macrophages’ polarization triggered by CMGNPs can be abolished after adding TLR4 inhibitor,CLI095,suggesting that TLR4 is involved in CMGNP-induced inflammation.

关键词： CMGNPs Macrophage Polarization Pro-inflammatory cytokines TLR 4 pathway

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Effect of hepatocyte growth factor signaling pathway activation on Plasmodium berghei infection

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Asian Pacific Journal of Tropical Medicine 2010年第3期3卷 169-172页

作者： Nantian Zhong fengying huang Guanghong Tan Jiege Jiao Yingzhi Lin CaichunWang Hua Wang Songlin Zhou Yonghao huang Fan Chen Yingying Lin Hainan Provincial Key Laboratory of Tropical Medicine Hainan Medical University Department of Histology and Embryology Hainan Medical University

Objective:To explore the effect of hepatocyte growth factor signaling pathway activation on Plasmodium berghei infection.Methods:In this study,hepatocyte growth factor was detected by ELISA and Western blotting assay.Hepatocyte injury was detected by FITC-dextran absorption assay,and hepatocyte growth factor expression was shown to be expressed in the same injury cells by immunofluorescence against hepatocyte growth factor.In addition,Activation of hepatocyte growth factor and its receptor signaling pathway was detected with immunoprecipitation and detection of phosphorylation status.Results:It was found that injury of hepatocytes by sporozoite migration induced the secretion of hepatocyte growth factor and it was hepatocyte growth factor that rendered hepatocytes susceptible to Plasmodium sporozoite infection.In addition,hepatocyte infections depended on activation of the hepatocyte growth factor and its receptor signaling pathway.Conclusions:Our results indicate that hepatocyte growth factor and its receptor may possibly be potential targets for new approaches to malaria treatment.

关键词： Hepatocyte growth factor Malaria Plasmodium sporozoite Signaling pathway

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人文护理学课程虚拟教研室建设与探索

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中华护理教育 2024年第6期21卷 658-662页

作者：罗雅文李卡卿平黄雷魏利琴刘艳黄浩张凤英四川大学华西医院/华西护理学院成都市610041

在信息化和智能化的时代背景下,虚拟教研室建设成为我国高校应对教育改革需要、创新发展基层教学组织的必然选择,是落实立德树人根本任务、整合教学资源、提升人才培养质量的新路径。四川大学联合国内10余所院校共同建设人文护理学课程... 详细信息

在信息化和智能化的时代背景下,虚拟教研室建设成为我国高校应对教育改革需要、创新发展基层教学组织的必然选择,是落实立德树人根本任务、整合教学资源、提升人才培养质量的新路径。四川大学联合国内10余所院校共同建设人文护理学课程虚拟教研室,围绕课程资源、教学团队、教学载体、教学运行等建设要素进行探索,构建了以人文护理学课程体系、课程思政元素集、跨专业跨院校跨地区高水平人文护理学教学团队、共享教学资源、线上线下虚实结合的护理教研活动为特色的教研新模式,全面服务西南西北护理院校,提升护理教学效能。

关键词：教育,护理虚拟教研室人文护理学

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高职院校机电一体化技术专业课程思政改革创新研究

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天津职业大学学报 2024年第3期33卷 75-80页

作者：李淼黄宇婷周凤颖黄欢杨永强北京工业职业技术学院北京100042

随着国家对高等职业教育质量要求的不断提高,高职院校机电一体化技术专业作为培养现代制造业人才的重要基地,课程思政改革创新尤为重要。针对当前机电一体化技术专业教学中思政元素融入不够、思政教育评估与反馈机制不完善等问题,深入... 详细信息

随着国家对高等职业教育质量要求的不断提高,高职院校机电一体化技术专业作为培养现代制造业人才的重要基地,课程思政改革创新尤为重要。针对当前机电一体化技术专业教学中思政元素融入不够、思政教育评估与反馈机制不完善等问题,深入探讨思政教育与专业教育的融合路径与实践模式。通过优化课程结构、创新教学方法、加强实践教学、完善成效评估等举措,有效提升学生的思政素养和职业素养。总结了成功经验,对其他专业的课程思政改革创新具有一定的借鉴意义。

关键词：高职院校机电一体化技术课程思政改革创新教学策略

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基于WGS构建葡萄高密度遗传图谱及抗炭疽病QTL定位

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中外葡萄与葡萄酒 2024年第3期 30-37页

作者：潘凤英曲俊杰黄羽韦荣福郭泽西尹玲孙大运广西壮族自治区农业科学院/广西作物遗传改良生物技术重点开放实验室广西南宁530007 广西农业科学院葡萄与葡萄酒研究所广西南宁530007

葡萄炭疽病是一种危害葡萄成熟果实的真菌病害,严重影响葡萄的产量和品质。美洲种葡萄具有适应南方湿热气候且高抗炭疽病的特点,因此构建美洲葡萄高密度分子遗传图谱,定位其抗炭疽病QTL,利用其中的抗病基因指导葡萄抗病育种具有重要意... 详细信息

葡萄炭疽病是一种危害葡萄成熟果实的真菌病害,严重影响葡萄的产量和品质。美洲种葡萄具有适应南方湿热气候且高抗炭疽病的特点,因此构建美洲葡萄高密度分子遗传图谱,定位其抗炭疽病QTL,利用其中的抗病基因指导葡萄抗病育种具有重要意义。本研究以美洲葡萄‘C30-5-1’165株自交后代为作图群体,基于全基因组测序(WGS)开发SNP标记,构建遗传图谱,并对葡萄抗炭疽病QTL进行分析。构建的葡萄分子遗传图谱总遗传距离为3138.74 cM,相邻标记间平均遗传距离为0.98 cM,分布均匀。根据群体炭疽病抗性表型数据,在11号连锁群上检测到1个炭疽病抗性相关QTL,命名为Cgr2,可解释表型变异5.0%,是一个微效QTL。与葡萄参考基因组比对分析发现,该QTL区域包含2个候选抗性基因,分别编码UDP糖基转移酶和类黄酮3’-单加氧酶,它们可能对葡萄炭疽病抗性起重要作用。

关键词：葡萄遗传图谱抗炭疽病 QTL定位

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概念图在护理实习生应急能力培训中的应用

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当代护士（上旬刊） 2024年第5期31卷 160-164页

作者：王燕罗江黄晓婷赵峰英福建医科大学附属第一医院福州350005 福建医科大学福州350122

目的运用概念图开展护理实习生应急能力培训,为临床实践教学及相关研究提供参考。方法根据需求对某三甲医院护理实习生进行应急能力培训,培训采用传统教学和概念图教学两种方式,对培训前后两届学生的应急能力进行调查和比较。结果护理... 详细信息

目的运用概念图开展护理实习生应急能力培训,为临床实践教学及相关研究提供参考。方法根据需求对某三甲医院护理实习生进行应急能力培训,培训采用传统教学和概念图教学两种方式,对培训前后两届学生的应急能力进行调查和比较。结果护理实习生应急能力处于中低水平。培训后,护理实习生的应急能力均有所提升(P<0.05),但与传统教学模式相比,概念图教学培训后护理实习生的应急能力提升更明显(P<0.05)。结论应用概念图教学可有效提升护理实习生的应急能力的培训效果。教学医院可以采用多种方式教学,优化教学形式,提高护理实习生应急能力。

关键词：概念图护理实习生应急能力

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