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Isolation and identification of multidrug-resistant Staphylococcus haemolyticus from a laboratory-breeding mouse

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Asian Pacific Journal of Tropical Medicine 2011年第6期4卷 421-425页

作者： fengying huang Qiuping Meng Guanghong Tan Yonghao huang Hua Wang Wenli Mei Haofu Dai Hainan Provincial Key Laboratory of Tropical Medicine Hainan Medical College Agriculture School Hainan University Biological Technology Institute Chinese Academy of Tropical Agricultural Sciences

Objective:To analysis and identify a bacterium strain isolated from laboratory breeding mouse far away from a ***:Phenotype of the isolate was investigated by conventional microbiological methods,including Gram-staining,colony morphology,tests for haemolysis, catalase,coagulase,and antimicrobial susceptibility *** meek and 16S rRNA genes were amplified by the polymerase chain reaction(PCR) and *** base sequence of the PCR product was compared with known 16S rRNA gene sequences in the CenBank database by phylogenetic analysis and multiple sequence ***:The isolate in this study was a gram positive,coagulase negative,and catalase positive *** isolate was resistant to oxacillin,methicillin,penicillin,ampicillin,cefazolin,cipr of loxacin erythromycin,et *** results indicated that the isolate was meek gene positive and its 16S rRNA was 1465 *** analysis of the resultant 16S rRNA indicated the isolate belonged to genus Saphylococcus,and multiple sequence alignment showed that the isolate was Saphylococcus haemolyticus with only one base difference from the corresponding 16S rRNA deposited in the ***: 16S rRNA gene sequencing is a suitable technique for non-specialist *** animals are possible sources of lethal pathogens,and researchers must adapt protective measures when they manipulate animals.

关键词： 16S rRNA Gene sequences analysis Staphylococcus haemolyticus Multidrug resistant

A novel therapeutic vaccine based on graphene oxide nanocomposite for tumor immunotherapy

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Chinese Chemical Letters 2022年第8期33卷 4089-4095页

作者： Liming Zhang Lingfeng Xu Yi Wang Jieyu Liu Guanghong Tan fengying huang Nongyue He Zhuoxuan Lu Key Laboratory of Tropical Translational Medicine of Ministry of Education&Hainan Provincial Key Laboratory of Tropical Medicine Hainan Medical UniversityHaikou 571199China State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Department of Clinical Laboratory HwaMei HospitalUniversity of Chinese Academy of SciencesNingbo 315000China

With an intensive understanding of the mechanism of immune system,developing a therapeutic tumor vaccine is one of the most perspective strategy of cancer *** this study,we report a facile approach to prepare graphene oxide(GO)-based therapeutic *** model antigen(ovalbumin,OVA)and adjuvant(CpG ODN),are conjugated with GO-PEI nanosheet through electrostatic *** addition of PEG can improve biocompatibility and prevent nanoparticle *** prepared GO-based nanovaccine,GO-PEI-OVA-PEG-CpG,exhibits good biocompatibility and low toxicity both in vivo and in *** importantly,it can efficiently induce the maturation of dendritic cells(DCs),the enhancement of antigen cross-presentation ability,and the amplification of cytokine production of immune ***,this nanovaccine shows a remarkable therapeutic effect against preestablished B16-OVA-melanoma tumors,which can significantly inhibit tumor growth and prolong the survival time of the OVA-expressed tumor-bearing ***,combining GO-PEI-OVA-PEG-CpG with NLG919,an IDO-1(indoleamine-2,3-dioxygenase)inhibitor which can regulate the tumor microenvironment,displays a synergistic therapeutic *** findings indicate the GO-PEI-OVA-PEG-CpG nanovaccine actively induces an antigen-specific antitumor immune response and it combined with NLG919 could achieve better therapeutic outcomes.

关键词： Nanovaccine Graphene Oxide Adjuvant Tumor-specific antigen NLG919

Cy5.5-MSA-G250 nanoparticles(CMGNPs)induce M1 polarity of RAW264. 7 macrophage cells via TLR4-dependent manner

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Chinese Chemical Letters 2019年第6期30卷 1320-1324页

作者： Zhuoxuan Lu Lingfeng Xu Nongyue He fengying huang Tiefeng Xu Li Li Yanwei Zhang Liming Zhang Key Laboratory of Tropical Disease and Translational Medicine of the Ministry of Education&Hainan Provincial Key Laboratory of Tropical Medicine Hainan Medical College Haikou 571101 China Hunan Key Laboratory of Biomedical Materials and Devices Hunan University of Technology Zhuzhou 412008 China The First Affiliated Hospital and the Oncological Institute of Hainan Medical College Haikou 571101 China

Cy5.5-MSA-G250 nanoparticles(CMGNPs)had been proved to have unique advantages for cancer treatment,including excellent photothermal performance,tumor cell-selective cytotoxicity,direct visualization,and good ***,to cellular systems,the CMGNPs are considered as fo reign invaders,and the effect of CMGNPs on immunity system is still ***,more efforts are needed to understand the role of CMGNPs on the immunity *** this study,we attempted to screen the pro-inflammatory responses on RAW264.7 macrophages after treated with the *** vitro experiments clearly showed that CMGNPs not only enhances phagocytosis capacity of RAW264.7 cells,but also promotes Ml polarization,associated with changes in cell morphology and increased expression of inflammatory *** ability to induce Ml polarization may be beneficial to CMGNPs to achieve better anticancer effects in clinical ***,the observed Ml macrophages’ polarization triggered by CMGNPs can be abolished after adding TLR4 inhibitor,CLI095,suggesting that TLR4 is involved in CMGNP-induced inflammation.

关键词： CMGNPs Macrophage Polarization Pro-inflammatory cytokines TLR 4 pathway

Effect of hepatocyte growth factor signaling pathway activation on Plasmodium berghei infection

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Asian Pacific Journal of Tropical Medicine 2010年第3期3卷 169-172页

作者： Nantian Zhong fengying huang Guanghong Tan Jiege Jiao Yingzhi Lin CaichunWang Hua Wang Songlin Zhou Yonghao huang Fan Chen Yingying Lin Hainan Provincial Key Laboratory of Tropical Medicine Hainan Medical University Department of Histology and Embryology Hainan Medical University

Objective:To explore the effect of hepatocyte growth factor signaling pathway activation on Plasmodium berghei ***:In this study,hepatocyte growth factor was detected by ELISA and Western blotting *** injury was detected by FITC-dextran absorption assay,and hepatocyte growth factor expression was shown to be expressed in the same injury cells by immunofluorescence against hepatocyte growth *** addition,Activation of hepatocyte growth factor and its receptor signaling pathway was detected with immunoprecipitation and detection of phosphorylation ***:It was found that injury of hepatocytes by sporozoite migration induced the secretion of hepatocyte growth factor and it was hepatocyte growth factor that rendered hepatocytes susceptible to Plasmodium sporozoite *** addition,hepatocyte infections depended on activation of the hepatocyte growth factor and its receptor signaling ***:Our results indicate that hepatocyte growth factor and its receptor may possibly be potential targets for new approaches to malaria treatment.

关键词： Hepatocyte growth factor Malaria Plasmodium sporozoite Signaling pathway

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钢轨表面裂纹涡流检测定量评估方法

中国铁道科学 2017年第2期38卷 28-33页

作者：黄凤英中国铁道科学研究院金属及化学研究所北京100081

采用ANSYS软件,针对1块表面有长9.0 mm、宽0.2 mm、深度分别为0.1,0.2,0.5,1.0,1.5,2.0和5.0mm裂纹的钢板建立涡流检测模型,仿真计算表面裂纹深度与磁感应强度的关系,得到近似服从指数关系的拟合曲线。在实验室制作钢轨表面裂纹试块,钢... 详细信息

采用ANSYS软件,针对1块表面有长9.0 mm、宽0.2 mm、深度分别为0.1,0.2,0.5,1.0,1.5,2.0和5.0mm裂纹的钢板建立涡流检测模型,仿真计算表面裂纹深度与磁感应强度的关系,得到近似服从指数关系的拟合曲线。在实验室制作钢轨表面裂纹试块,钢轨表面垂直裂纹试块的裂纹深度分别为0.5,1.0,2.0和5.0mm,钢轨表面斜裂纹试块的等效垂直深度分别为1.0和2.0mm且倾角分别为15°,30°,45°和60°;按照指数关系对试块表面裂纹深度与涡流检测信号电压幅值的关系进行拟合,得到可靠度高的指数函数式。截取1段表面带有自然裂纹的钢轨进行涡流检测试验,再利用拟合得到的指数函数式计算钢轨表面裂纹的深度,并与实际测量结果进行对比。结果表明:利用拟合的指数函数式评估深度约为2mm以内的钢轨表面裂纹时,表面裂纹深度的计算误差不超过10%,但是随后误差越来越大,因此采用涡流检测方法能够对2mm以内深度的钢轨表面裂纹进行有效检测及定量评估。

关键词：钢轨探伤表面裂纹涡流检测指数函数非接触无损检测

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静态偏置磁场对电磁超声换能器灵敏度的影响

机械工程学报 2011年第10期47卷 1-7页

作者：黄凤英周正干北京航空航天大学无损检测及机电工程研究所北京100191

电磁超声换能器(Electromagnetic acoustic transducer,EMAT)灵敏度低,其静态偏置磁场是影响EMAT灵敏度的关键因素。为提高EMAT的灵敏度,利用有限元软件ANSYS计算EMAT中永磁体产生的磁感应强度,得到磁感应强度的分布规律,并结合试验方... 详细信息

电磁超声换能器(Electromagnetic acoustic transducer,EMAT)灵敏度低,其静态偏置磁场是影响EMAT灵敏度的关键因素。为提高EMAT的灵敏度,利用有限元软件ANSYS计算EMAT中永磁体产生的磁感应强度,得到磁感应强度的分布规律,并结合试验方法获得永磁体的优化结构参数,以指导EMAT结构的优化设计。研究结果表明,当永磁体与线圈的间距减小时,超声振动回波幅值增大,但对线圈干扰加大,在两者之间取折中能够得到较高信噪比的回波信号;永磁体厚度对换能器灵敏度产生严重影响,当厚度增大,磁感应强度增强,但趋势逐渐变缓。聚磁板的应用有利于集中磁感应强度,提高回波信号的信噪比。

关键词：电磁超声换能器静态偏置磁场永磁体

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Activin及其受体与相关的妇产科疾病

中南大学学报（医学版） 2010年第8期35卷 896-902页

作者：李红云黄凤英中南大学湘雅二医院妇产科长沙410011

激活素(activin,ACT)是转化生长因子β(TGF-β)超家族球蛋白成员,是抑制素β亚基的二聚体,其受体为膜受体,activin通过其受体系统的信号传导发挥其生物学功能。近年来,许多研究表明activin具有广泛的生物学活性,是调节组织细胞功能的基... 详细信息

激活素(activin,ACT)是转化生长因子β(TGF-β)超家族球蛋白成员,是抑制素β亚基的二聚体,其受体为膜受体,activin通过其受体系统的信号传导发挥其生物学功能。近年来,许多研究表明activin具有广泛的生物学活性,是调节组织细胞功能的基本介质,参与维持细胞正常功能,并在卵巢上皮性肿瘤、子宫内膜癌、子痫前期、多囊卵巢综合征、子宫内膜异位症等多种妇产科疾病组织中表达异常,影响着这些疾病的发生发展。

关键词： activin activin受体妇产科疾病

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超外差接收相敏检波在电磁超声检测中的应用

北京航空航天大学学报 2011年第3期37卷 253-258页

作者：周正干黄凤英倪先胜北京航空航天大学机械工程及自动化学院北京100191

为了提高电磁超声检测接收信号的信噪比,研究和设计了基于超外差接收正交相敏检波技术的电磁超声检测系统.应用该系统分别对铝合金、碳钢和不锈钢试样进行了检测,对接收信号进行了正交相敏检波处理,将处理后的信号同原始接收射频信号进... 详细信息

为了提高电磁超声检测接收信号的信噪比,研究和设计了基于超外差接收正交相敏检波技术的电磁超声检测系统.应用该系统分别对铝合金、碳钢和不锈钢试样进行了检测,对接收信号进行了正交相敏检波处理,将处理后的信号同原始接收射频信号进行了对比,分析了中频放大器和相敏检波器中低通滤波器的带宽设置对检测结果的影响.研究结果表明:超外差接收正交相敏检波技术在铝合金、碳钢和不锈钢等非铁磁性或铁磁性金属材料的电磁超声检测中可以起到去噪、提高信噪比的作用.

关键词：电磁超声换能器超外差接收正交相敏检波

Temporal Involvement of αCaMK2 in conditioned taste aversion

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Temporal Involvement of αCaMK2 in conditioned taste aversio...