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Mercury mass flow in iron and steel production process and its implications for mercury emission control

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Journal of Environmental Sciences 2016年第5期28卷 293-301页

作者： fengyang wang Shuxiao wang Lei Zhang Hai Yang Wei Gao Qingru Wu Jiming Hao State Key Joint Laboratory of Environment Simulation and Pollution Control School of Environment Tsinghua UniversityBeijing 100084 China State Environmental Protection Key Laboratory of Sources and Control of Air Pollution Complex Beijing 100084 China

The iron and steel production process is one of the predominant anthropogenic sources of atmospheric mercury emissions worldwide. In this study, field tests were conducted to study mercury emission characteristics and mass flows at two iron and steel plants in China. It was found that low-sulfur flue gas from sintering machines could contribute up to41% of the total atmospheric mercury emissions, and desulfurization devices could remarkably help reduce the emissions. Coal gas burning accounted for 17%–49% of the total mercury emissions, and therefore the mercury control of coal gas burning, specifically for the power plant burning coal gas to generate electricity, was significantly important. The emissions from limestone and dolomite production and electric furnaces can contribute29.3% and 4.2% of the total mercury emissions from iron and steel production. More attention should be paid to mercury emissions from these two processes. Blast furnace dust accounted for 27%–36% of the total mercury output for the whole iron and steel production process. The recycling of blast furnace dust could greatly increase the atmospheric mercury emissions and should not be conducted. The mercury emission factors for the coke oven,sintering machine and blast furnace were 0.039–0.047 g Hg/ton steel, and for the electric furnace it was 0.021 g Hg/ton steel. The predominant emission species was oxidized mercury, accounting for 59%–73% of total mercury emissions to air.

关键词： Iron and steel plant Mercury Emission characteristics Mass flow Emission control

Measurement of tropospheric HO_(2) radical using fluorescence assay by gas expansion with low interferences

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Journal of Environmental Sciences 2021年第1期33卷 40-50页

作者： Yihui wang Renzhi Hu Pinhua Xie Hao Chen fengyang wang Xiaoyan Liu JianGuo Liu Wenqing Liu School of Enuironmental Science and Optoelectronic Technology University of Science and Technology of ChinaHefei 230026AnhuiChina Key Laboratory of Environmental Optics and Technology Anhui Institute of optics and Fine MechanicsChineseAcademy of SciencesHefei 230031AnhuiChina CAS Center for Excellence in Regional Atmospheric Enuvironment Institute of Urban EnuironmentChineseAcademy of SciencesXiamen 361000FujianChina University of Chinese Academy of Sciences Beijing 100049BeijingChina College of Mechanical Engineering Suzhou University of Science and TechnologySuzhou 215009jiangsuChina College of Pharmacy Anhui Medical UniversityHefei 230032AnhuiChina

An instrument to detect atmospheric HO_(2) radicals using fluorescence assay by gas expansion(FAGE)technique has been ***_(2) is measured by reaction with NO to form OH and subsequent detection of OH by laser-induced fluorescence at low *** system performance has been improved by optimizing the expansion distance and pressure,the influence factors of HO_(2) conversion efficiency are also *** interferences of RO_(2) radicals were investigated by determining the conversion efficiency of RO_(2) to OH during the measurement of HO_(2).The dependence of the conversion of HO_(2) on NO concentration was investigated,and low HO_(2) conversion efficiency was selected to realize the ambient HO_(2) measurement,where the conversion efficiency of RO_(2) derived by propane,ethene,isoprene and methanol to OH has been reduced to less than 6%in the ***,no significant interferences from PM_(2.5) and NO were found in the ambient HO_(2) *** detection limits for HO_(2)(S/N=2)are estimated to 4.8×10^5 cm^-3 and 1.1×10^6 cm^-3(ρHO_(2)=20%)under night and noon conditions,with 60 sec signal integration *** instrument was successfully deployed during STORM-2018 field campaign at Shenzhen graduate school of Peking *** concentration of atmospheric HOx radical and the good correlation of OH with j(O1D)was obtained *** diurnal variation of HOx concentration shows that the OH maximum concentration of those days is about 5.3×10^6 cm^-3 appearing around 12:00,while the HO_(2) maximum concentration is about 4.2×10^8 cm^-3 appearing around 13:30.

关键词： HO_(2) radical FAGE Interference Environmental measurement

A hydroxyl radical detection system using gas expansion and fast gating laser-induced fluorescence techniques

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Journal of Environmental Sciences 2018年第3期30卷 190-200页

作者： Hao Chen Renzhi Hu Pinhua Xie Xingbiao Xing Liuyi Ling Zhiyan Li fengyang wang Yihui wang Jianguo Liu Wenqing Liu Department of Precision Machinery and Precision Instrumentation University of Science and Technology of China Key Laboratory of Environmental Optics and Technology Anhui Institute of Optics and Fine Mechanics Chinese Academy of Sciences CAS Center for Excellence in Regional Atmospheric Environment Institute of Urban Environment Chinese Academy of Sciences Institute of Electric and Information Technology Anhui University of Science and Technology

An OH radical measurement instrument based on Fluorescence Assay by Gas Expansion（FAGE）has been developed in our *** air is introduced into a low-pressure fluorescence cell through a pinhole aperture and irradiated by a dye laser at a high repetition rate of 8.5 k *** OH radical is both excited and detected at 308 nm using A-X（0,0）*** satisfy the high efficiency needs of fluorescence collection and detection,a 4-lens optical system and a self-designed gated photomultiplier（PMT）is used,and gating is actualized by switching the voltage applied on the PMT dynodes.A micro channel photomultiplier（MCP）is also prepared for fluorescence *** the weak signal is accumulated by a photon counter in a specific *** OH radical excitation spectrum range in the wavelength of 307.82–308.2 nm is detected and the excited line for OH detection is determined to be Q1（2）*** calibration of the FAGE system is researched by using simultaneous photolysis of H2O and *** minimum detection limit of the instrument using gated PMT is determined to be 9.4×10~5molecules/cm^3,and the sensitivity is 9.5×10^（-7）cps/（OH·cm^（-3））,with a signal-to-noise ratio of 2 and an integration time of 60 sec,while OH detection limit and the detection sensitivity using MCP is calculated to be 1.6×10~5molecules/cm^3and 2.3×10^（-6）cps/（OH·cm^（-3））.The laboratory OH radical measurement is carried out and results show that the proposed system can be used for atmospheric OH radical measurement.

关键词： OH radical Laser-induced fluorescence Fluorescence assay by gas expansion Photomultiplier Photon counting

全外显子组测序在有免疫缺陷患儿夭折史夫妻双方隐性遗传病携带情况推断分析中的应用

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中华医学遗传学杂志 2024年第2期41卷 134-139页

作者：张冰杨科张玉薇娄桂予祁娜雷星星王凤阳康冰廖世秀河南省人民医院/河南省人民医院医学遗传研究所郑州450003

目的探讨全外显子测序技术在具有原发性免疫缺陷(PID)患儿夭折史但无先证者的夫妻双方隐性遗传病携带推断分析中的应用。方法收集2017年2月至2021年12月就诊于河南省人民医院因有疑似PID患儿生育史进行遗传学检查的4对夫妻的临床资料。... 详细信息

目的探讨全外显子测序技术在具有原发性免疫缺陷(PID)患儿夭折史但无先证者的夫妻双方隐性遗传病携带推断分析中的应用。方法收集2017年2月至2021年12月就诊于河南省人民医院因有疑似PID患儿生育史进行遗传学检查的4对夫妻的临床资料。对夫妻双方外周血样进行全外显子测序(WES),就候选变异用Sanger测序或荧光定量PCR进行验证,并进一步对家系中的胎儿进行产前诊断。结果WES共检出6个与病史相关的致病或疑似致病的基因变异,包含有IL2RG、BTK、CYBB、DUOX2等基因,变异类型包括点变异、小片段缺失及外显子缺失等。其中DUOX2基因c.1265G>A、c.3329G>A,IL2RG基因c.676C>T为已报道致病性变异。IL2RG基因Exon58del、BTK基因c.184185delAC、CYBB基因c.472A>T既往未见报道。根据美国医学遗传学与基因组学学会变异评级指南,IL2RG基因Exon58del、BTK基因c.184185delAC、CYBB基因c.472A>T评级均为可能致病性变异(PVS1+PM2Supporting+PP4)。其中3对夫妻再次妊娠后进行了产前诊断,结果显示胎儿BTK基因c.184185、CYBB基因c.472、IL2RG基因c.676位点均为野生型,胎儿出生1年后随访未发现异常。结论全外显子组测序是具有免疫缺陷患儿夭折史夫妻双方隐性遗传病携带情况推断分析的一种重要手段,可提高阳性检出率。

关键词：免疫缺陷全外显子组测序基因变异隐性遗传病

重组腺相关病毒9型空壳率阴离子交换高效液相色谱检测方法的建立及验证

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中国生物制品学杂志 2024年第5期37卷 519-526页

作者：史新昌王凤阳刘亚辉周勇刘宾中国食品药品检定研究院、国家卫生健康委员会生物技术产品检定方法及其标准化重点实验室、国家药品监督管理局生物制品质量研究与评价重点实验室北京100050 上海泰昶生物技术有限公司上海201413 上海天泽云泰生物医药有限公司上海201203

目的建立检测重组腺相关病毒9型(recombinant adeno-associated virus type 9,rAAV9)空壳率的阴离子交换高效液相色谱(anion-exchange high-performance liquid chromatography,AEX-HPLC)法,并进行验证。方法采用基于电荷差异的AEX-HPLC法,通过对流动相洗脱梯度、流动相pH、色谱柱柱温、流速、样品浓度、进样体积和荧光检测器检测波长进行优化,建立检测rAAV9空壳与实心病毒比例的方法,并对方法的专属性、线性、检测限、定量限、精密度和准确度进行验证,确认方法可行性。结果利用CIMac AAV full/empty-0.1 mL分析柱,以20 mmol/L 1,3-二[三(羟甲基)甲氨基]丙烷(BIS-Tris propane,BTP)为流动相A,20 mmol/L BTP+1 mol/LNaCl为流动相B进行梯度洗脱,流动相pH均为9.0,柱温20℃,流速1 mL/min,样品浓度4×10^(12)vg/mL,进样体积10μL,激发波长为280 nm,发射波长为330 nm,可实现rAAV9空壳与实心病毒的基线分离及定量检测。方法验证结果表明,制剂缓冲液无干扰,专属性较好;rAAV9在(1.6~8)×10^(12)vg/mL范围内线性关系良好,r=0.993;检测限为5×10^(10)vg/mL,定量限为1×10^(11) vg/mL;重复性和中间精密度RSD分别为2.95%和2.10%;准确率均不低于80%。结论建立了一种高灵敏度且可快速检测rAAV9空壳与实心病毒比例的AEX-HPLC法,可用于基因治疗产品空壳率的分析及质量控制。

关键词：重组腺相关病毒基因治疗阴离子交换高效液相色谱法空壳病毒实心病毒质量控制

Defective arginine metabolism impairs mitochondrial homeostasis in Caenorhabditis elegans

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Journal of Genetics and Genomics 2020年第3期47卷 145-156页

作者： Ruofeng Tang Xin wang Junxiang Zhou Fengxia Zhang Shan Zhao Qiwen Gan Liyuan Zhao fengyang wang Qian Zhang Jie Zhang Guodong wang Chonglin Yang State Key Laboratory of Natural Resource Conservation and Utilization in Yunnan Center for Life ScienceSchool of Life SciencesYunnan UniversityKunming650021China State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing100101China Graduate University of Chinese Academy of Sciences Beijing100049China State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing100101China

Arginine catabolism involves enzyme-dependent reactions in both mitochondria and the cytosol,defects in which may lead to hyperargininemia,a devastating developmental *** is largely unknown if defective arginine catabolism has any effects on *** we report that normal arginine catabolism is essential for mitochondrial homeostasis in Caenorhabditis *** of the arginase gene argn-1 lead to abnormal mitochondrial enlargement and reduced adenosine triphosphate(ATP)production in C elegans hypodermal ***-1 localizes to mitochondria and its loss causes arginine accumulation,which disrupts mitochondrial *** expression of human ARGl or ARG2 rescued the mitochondrial defects of argn-1 ***,genetic inactivation of the mitochondrial basic amino acid transporter SLC-25A29 or the mitochondrial glutamate transporter SLC-25A18.1 fully suppressed the mitochondrial defects caused by argn-1 *** findings suggest that mitochondrial damage probably contributes to the pathogenesis of hyperargininemia and provide clues for developing therapeutic treatments for hyperargininemia.

关键词： Arginine Arginase Hyperargininemia Mitochondrial homeostasis C.elegans

山羊NPC1基因核心启动子鉴定与转录调控分析

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中国动物传染病学报 2024年第3期32卷 154-163页

作者：李崇瑞陈思翟哲王雪梅吴艳茹刘志勇蒋俊明满初日嘎杜丽王凤阳海南大学动物科技学院海南省热带动物繁育与疫病研究重点实验室海口市动物基因工程重点实验室海口570228

NPC1基因编码的C型尼曼匹克蛋白1(NPC1)参与脂筏形成、病原微生物内吞以及内吞体的胞内转运等过程。本试验旨在阐明海南黑山羊NPC1基因的转录调控机制,为研究病原微生物与宿主细胞互作提供理论参考。首先,以山羊NPC1基因1466 bp启动子... 详细信息

NPC1基因编码的C型尼曼匹克蛋白1(NPC1)参与脂筏形成、病原微生物内吞以及内吞体的胞内转运等过程。本试验旨在阐明海南黑山羊NPC1基因的转录调控机制,为研究病原微生物与宿主细胞互作提供理论参考。首先,以山羊NPC1基因1466 bp启动子序列为模板,构建9个启动子5'端连续缺失的双荧光素酶报告载体,筛选NPC1基因核心启动子区;继而对核心启动子区的关键转录因子进行预测分析,构建转录因子结合位点缺失的双荧光素酶报告载体,筛选NPC1基因的关键转录因子结合位点。结果表明:山羊NPC1基因的核心启动子区位于转录起始位点上游-195 bp至-60 bp,并且该区域存在E2F3(-134/-120 bp)、SREBP-1(-107/-96 bp)、SP1(-68/-62 bp)等多个转录因子结合位点。双荧光素酶报告实验结果表明,缺失E2F3、SREBP-1、SP1三个转录因子结合位点均会使山羊NPC1基因启动子的转录活性显著下降(P<0.01),且缺失SREBP-1结合位点后NPC1基因转录活性下降最显著。提示,SREBP-1转录因子对海南黑山羊NPC1基因转录活性具有重要的调控作用。本试验成功鉴定山羊NPC1基因的核心启动子区(-195/-60 bp)及该区域的关键转录因子结合位点SREBP-1,为进一步研究山羊NPC1基因介导病原微生物与宿主互作分子机制提供理论基础。

关键词：海南黑山羊 NPC1基因转录调控核心启动子 SREBP-1

Two-Factor Fuzzy Time Series for Equipment Data Prediction

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Journal of Shanghai Jiaotong university(Science) 2018年第5期23卷 684-690页

作者： HE Shanhong RONG Baizhong QU Meng wang Shuangfei LI guanhuan wang fengyang WU Jin Suzhou Nuclear Power Research Institute Shenzhen 518026GuangdongChina

The data forecasting of plant equipment plays an important role in assurance of the safe and reliable operation of the plant equipment. Thus, it is necessary to improve the accuracy of data forecasting of the equipment. A new two-factor fuzzy time series algorithm is proposed to forecast the data of the plant *** method not only overcomes the limitations of one factor fuzzy time series algorithm, but also overcomes the drawbacks of traditional two-factor fuzzy time series algorithm. The collected data is used in the power plant to conduct experiments, where the metrics is Mean Absolute Percentage Error(MAPE). The results show that this method is superior to the existing two-factor fuzzy time series algorithms, and yields good results in the equipment prediction.

关键词： equipment prediction fuzzy time series two-factor fuzzy time series

A pair of transporters controls mitochondrial Zn^(2+) levels to maintain mitochondrial homeostasis

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Protein & Cell 2022年第3期13卷 180-202页

作者： Tengfei Ma Liyuan Zhao Jie Zhang Ruofeng Tang Xin wang Nan Liu Qian Zhang fengyang wang Meijiao Li Qian Shan Yang Yang Qiuyuan Yin Limei Yang Qiwen Gan Chonglin Yang State Key Laboratory of Conservation and Utilization of Bio-resources in Yunnan and Center for Life Sciences School of Life SciencesYunnan UniversityKunming 650091China State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China

Zn^(2+)is required for the activity of many mitochondrial proteins,which regulate mitochondrial dynamics,apoptosis and ***,it is not understood how the proper mitochondrial Zn^(2+)level is achieved to maintain mitochondrial *** Caenorhabditis elegans,we reveal here that a pair of mitochondrion-localized transporters controls the mitochondrial level of Zn^(2+).We demonstrate that SLC-30A9/ZnT9 is a mitochondrial Zn^(2+)*** of SLC-30A9 leads to mitochondrial Zn^(2+)accumulation,which damages mitochondria,impairs animal development and shortens the life *** further identify SLC-25A25/SCaMC-2 as an important regulator of mitochondrial Zn^(2+)*** of SLC-25A25 suppresses the abnormal mitochondrial Zn^(2+)accumulation and defective mitochondrial structure and functions caused by loss of ***,we reveal that the endoplasmic reticulum contains the Zn^(2+)pool from which mitochondrial Zn^(2+)is *** findings establish the molecular basis for controlling the correct mitochondrial levels for normal mitochondrial structure and functions.

关键词： mitochondria Zn^(2+)transporter C.elegans ER-mitochondrial contact development