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Establishment and Application of Heminested RT-PCR Assay for Detection of Mosquito-Borne Flavivirus—Guizhou Province,China,2018

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China CDC weekly 2021年第1期3卷 4-9页

作者： Zhijing Xue Ning Zhao Jun Wang Xiuping Song fengxia meng Wenqin Liang Jingzhu Zhou Dan Wang Zhong Zhang Qiyong Liu National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijingChina School of Basic Medical Sciences Shandong First Medical UniversityTaianShandongChina Guizhou Province Center for Disease Control and Prevention GuiyangGuizhouChina School of Life Sciences Shandong First Medical UniversityTaianShandongChina Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases TaianShandongChina

Introduction:Mosquito-borne flavivirus can lead to serious infectious diseases worldwide and cause high mortality and *** order to strengthen epidemiological investigation of flavivirus and meet the needs of early warning of diseases,a simple,rapid,and sensitive detection method needs to be established to prevent and control mosquito-borne ***:Using the NS5 gene of flavivirus in GenBank,3 universal primers targeting the conserved regions were *** complementary DNAs(cDNAs)of Japanese encephalitis virus(JEV),dengue virus(DENV),West Nile virus(WNV),and yellow fever virus(YFV)were used as the template to optimize the reaction conditions.A heminested reversetranscriptase polymerase chain reaction(hnRT-PCR)was established to verify the sensitivity and specificity of this method and to detect field-caught ***:Our results showed that this method exhibited better specificity,higher sensitivity,and the ability to detect multiple viruses *** lowest detection limit of JEV,DENV-2,YFV,and WNV was 3×10^(4),3×10^(6),3×10^(5),and 3×10^(4)copies/μL,***-borne flavivirus was successfully detected in the field-caught mosquito samples using the method established in this ***:The hnRT-PCR method established in this study can be employed for the rapid detection of flavivirus and provide technical support for early and rapid diagnosis of mosquito-borne flavivirus.

关键词： Guizhou specificity template

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Mutations and intron polymorphisms in voltage-gated sodium channel genes of different geographic populations of Culex pipiens pallens/Culex pipiens quinquefasciatus in China

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Infectious Diseases of Poverty 2024年第2期13卷 6-16页

作者： Wenyu Li Delong Ma Qunzheng Mu Xinxin Zhou Dongdong Hua Chunchun Zhao Qiyong Liu Jun Wang fengxia meng State Key Laboratory of Infectious Disease Prevention and Control Department of Vector Biology and ControlNational Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionWHO Collaborating Centre for Vector Surveillance and ManagementBeijing 102206People’s Republic of China Ningxia Center for Disease Prevention and Control YinChuan 750004NingXiaPeople’s Republic of China Jinan Shizhong Center for Disease Control and Prevention Jinan 250000ShandongChina Vanke School of Public Health Tsinghua UniversityBeijingPeople’s Republic of China Weifang No.2 People’s Hospital Weifang 261000ShandongPeople’s Republic of China Beijing Daxing Center for Disease Control and Prevention Beijing 102600People’s Republic of China Jinan Second Maternal and Child Health Hospital Jinan 250000ShandongChina

Background Culex pipiens pallens and Culex pipiens quinquefasciatus are the dominant species of Culex mosquitoes in China and important disease ***-term use of insecticides can cause mutations in the voltage-gated sodium channel(vgsc)gene of mosquitoes,but little is known about the current status and evolutionary origins of vgsc gene in different geographic ***,this study aimed to determine the current status of vgsc genes in *** and *** in China and to investigate the evolutionary inheritance of neighboring downstream introns of the vgsc gene to determine the impact of insecticides on long-term *** Sampling was conducted from July to September 2021 in representative habitats of 22 provincial-level administrative divisions in *** DNA was extracted from 1308 mosquitoes,the IIS6 fragment of the vgsc gene on the nerve cell membrane was amplified using polymerase chain reaction,and the sequence was used to evaluate allele frequency and knockdown resistance(kdr)*** 11 was used to construct neighborjoining(NJ)*** was used to build a TCS *** There were 6 alleles and 6 genotypes at the L1014 locus,which included the wild-type alleles TTA/L and CTA/L and the mutant alleles TTT/F,TTC/F,TCT/S and TCA/*** geographic populations with a kdr frequency less than 20.00%were mainly concentrated in the regions north of 38°N,and the geographic populations with a kdr frequency greater than 80.00%were concentrated in the regions south of 30°*** frequency increased with decreasing *** within the same latitude,the frequency of kdr in large cities is relatively *** were correlated with the number of *** mutant allele TCA/S has only one intron,the mutant allele TTT/F has three introns,and the wild-type allele TTA/L has 17 *** *** and *** have developed resistance to insecticides in most regions of *** nei

关键词： Culex pipiens pallens Culex pipiens quinquefasciatus vgsc gene Mutation Frequency Intron Haplotype China

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A novel strategy for screening mutations in the voltage-gated sodium channel gene of Aedes albopictus based on multiplex PCR-mass spectrometry minisequencing technology

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Infectious Diseases of Poverty 2023年第4期12卷 43-53页

作者： Qunzheng Mu Xin Zhao Fengfeng Li Wenyu Li Xinxin Zhou Xinchang Lun Yiguan Wang Dongdong Hua Qiyong Liu Di Xiao fengxia meng National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing102206People’s Republic of China Weifang No.2 People’s Hospital Weifang261000ShandongPeople’s Republic of China Weifang Medical College Weifang261000ShandongPeople’s Republic of China Beijing Daxing District Center for Disease Control and Prevention Beijing102600BeijingPeople’s Republic of China

Background The current prevention and control strategy forAedes albopictus heavily relies on comprehensive management, such as environmental management and chemical control. However, the wide application of pyrethroids has facilitated the development of insecticide resistance, primarily via mutations in the voltage-gated sodium channel (VGSC) gene. This study aims to develop a novel strategy for detecting mutations in the VGSC gene inAe. albopictus using multiplex PCR-mass spectrometry (MPCR-MS) minisequencing *** We established a new strategy for detecting mutations in the VGSC gene inAe. albopictus using MPCR-MS minisequencing technology. MPCR amplification and mass probe extension (MPE) were first used, followed by single nucleotide polymorphism (SNP) typing mass spectrometry, which allows the simultaneous detection of multiple mutation sites of the VGSC gene in 96 samples ofAe. albopictus. A total of 70 wild-collectedAe. albopictus were used to evaluate the performance of the method by comparing it with other *** Three target sites (1016, 1532, 1534) in the VGSC gene can be detected simultaneously by double PCR amplification combined with matrix-assisted laser desorption ionization-time-of-flight mass spectrometry, achieving a detection limit of 20 fg/μl. We applied this method to 70 wild-collectedAe. albopictus, and the obtained genotypes were consistent with the routine sequencing results, suggesting the accuracy of our *** MPCR-MS minisequencing technology provides a sensitive and high-throughput approach toAe. albopictus VGSC gene mutation screening. Compared with conventional sequencing, this method is economical and time-saving. It is of great value for insecticide resistance surveillance in areas with a high risk of vector-borne disease.

关键词： Aedes albopictus VGSC gene Mutation Single nucleotide polymorphisms Multiplex polymerase chain reaction-mass spectrometry mini-sequencing

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Epidemiological characteristics and temporal-spatial analysis of overseas imported dengue fever cases in outbreak provinces of China, 2005–2019

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Infectious Diseases of Poverty 2022年第1期11卷 91-91页

作者： Xinchang Lun Yiguan Wang Chunchun Zhao Haixia Wu Caiying Zhu Delong Ma Mingfang Xu Jun Wang Qiyong Liu Lei Xu fengxia meng State Key Laboratory of Infectious Diseases Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing102206People’s Republic of China 不详

Background Overseas imported dengue fever is an important factor in local outbreaks of this disease in the mainland of *** better prevent and control such local outbreaks,the epidemiological characteristics and temporal-spatial distribution of overseas imported dengue fever cases in provincial-level administrative divisions(PLADs)where dengue fever is outbreak in the mainland of China were *** Using the Chinese National Notifiable Infectious Disease Reporting Information System(CNNDS),we identified overseas imported dengue fever cases in dengue fever outbreak areas in the mainland of China from 2005 to 2019 to draw the epidemic curve and population characteristic distribution of overseas imported cases in each *** on spatial autocorrelation analysis of ArcGIS 10.5 and temporal-spatial scanning analysis of SaTScan 9.5,we analyzed the temporal-spatial distribution of overseas imported dengue fever in dengue fever outbreak areas in the mainland of *** A total of 11,407 imported cases,mainly from Southeast Asia,were recorded from 2005 to 2019 in these 13 *** which 62.1%were imported into Yunnan and Guangdong *** the imported cases,there were more males than females,mainly from the 21–50 age *** hot spots were concentrated in parts of Yunnan,Guangdong and Fujian *** found the cluster of infected areas were expanding *** Based on the analysis of overseas imported dengue cases in 13 PLADs of the mainland of China from 2005 to 2019,we obtained the epidemiological characteristics and spatial distribution of imported dengue *** controls need to pay attention to key population sectors,such as 21–50 years old men and education of key populations on dengue *** is a need to improve the awareness of the prevention and control of imported cases in border *** the same time,northern regions cannot relax their vigilance.

关键词： Imported case Dengue fever China Epidemiology Temporal-spatial distribution

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转Bt基因棉对棉铃虫抗虫性测定方法的研究

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南京农业大学学报 2000年第1期23卷 109-113页

作者：孟凤霞沈晋良周威君高聪芬唐灿明南京农业大学农业部病虫监测与治理重点开放实验室南京210095 南京农业大学农学系南京210095

采用喂饲法 ,建立了转Bt基因棉叶片和蕾对棉铃虫初孵幼虫的抗虫性测定方法及相应的指标。叶片喂饲法 :第 2～ 3展开叶用浸水的脱脂棉保湿放入玻璃或塑料容器中 ,接 5头初孵幼虫 ,用扎孔的塑料薄膜封口 ,在 (2 7± 1)℃条件下饲养 5d后... 详细信息

采用喂饲法 ,建立了转Bt基因棉叶片和蕾对棉铃虫初孵幼虫的抗虫性测定方法及相应的指标。叶片喂饲法 :第 2～ 3展开叶用浸水的脱脂棉保湿放入玻璃或塑料容器中 ,接 5头初孵幼虫 ,用扎孔的塑料薄膜封口 ,在 (2 7± 1)℃条件下饲养 5d后记录死亡率、存活幼虫发育龄期及危害级别。蕾喂饲法 :每蕾接 2头初孵幼虫 ,控制条件同上 ,喂饲 3d后 ,记录幼虫死亡率和存活幼虫发育龄期。以上方法的建立 ,为转Bt基因棉抗虫性的时空规律与害虫抗性选育和遗传规律的研究提供了较为可靠、简便的方法。

关键词：转Bt基因棉棉铃虫抗虫性测定方法蕾喂饲法

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多杀菌素及其抗药性机制研究进展

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中国媒介生物学及控制杂志 2015年第4期26卷 431-432页

作者：王燕燕孟凤霞高希武国家知识产权局专利局专利审查协作江苏中心江苏苏州215163 中国疾病预防控制中心传染病预防控制所中国农业大学

介绍了昆虫对多杀菌素的抗性发展情况,从生理生化机制、分子水平方面总结了多杀菌素的抗性机制研究进展,昆虫对多杀菌素的抗性机制至今尚未明确,研究其抗性机制,延缓抗性发展,具有重要意义。

关键词：多杀菌素抗药性代谢酶烟碱型乙酰胆碱受体

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里海伊蚊的抗药性研究

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中国媒介生物学及控制杂志 2015年第4期26卷 418-419页

作者：黄志光胡俊叶刚鲜军孟凤霞克拉玛依市疾病预防控制中心新疆克拉玛依834000 中国疾病预防控制中心传染病预防控制所

目的建立里海伊蚊的实验室饲养繁殖方法,了解克拉玛依地区里海伊蚊对常用杀虫剂的抗药性水平。方法常规饲养里海伊蚊,对繁殖出的子一代采用幼蚊浸渍法,检测其对常用杀虫剂的半数致死浓度（LC50）。结果在室温（26±1）℃和相对湿度40%~... 详细信息

目的建立里海伊蚊的实验室饲养繁殖方法,了解克拉玛依地区里海伊蚊对常用杀虫剂的抗药性水平。方法常规饲养里海伊蚊,对繁殖出的子一代采用幼蚊浸渍法,检测其对常用杀虫剂的半数致死浓度（LC50）。结果在室温（26±1）℃和相对湿度40%~60%的条件下,里海伊蚊卵孵化期为3 d,1~4龄幼虫期为7 d,从卵产下到羽化为11 d,一个卵荚孵化出110~120条幼虫。克拉玛依市敌敌畏、残杀威、溴氰菊酯、高效氯氰菊酯、双硫磷对里海伊蚊的LC50分别为0.2838、2.7752、0.0024、0.0126和0.0033 mg/L。结论克拉玛依地区里海伊蚊常用杀虫剂的LC50本底值比已经报道过的白纹伊蚊要高。为了避免交互抗性的产生,该地区里海伊蚊的防治应采取以环境治理和生物防治为主、化学防治为辅的综合治理措施,注重统筹用药和农业合理规划,定期进行抗药性监测,做到科学合理用药。

关键词：里海伊蚊抗药性浸渍法

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Large-Amplitude Oscillations of Hyperelastic Cylindrical Membrane Under Thermal-Mechanical Fields

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Acta Mechanica Solida Sinica 2022年第2期35卷 303-315页

作者： Wenzheng Zhang Datian Niu fengxia Zhao College of Science Dalian Minzu UniversityDalian 116600China Department of Information Engineering Qinhuangdao Vocational and Technical CollegeQinhuangdao 066100China

In this paper,the nonlinear dynamic behaviors of a hyperelastic cylindrical membrane composed of the incompressible Ogden material are examined,where the membrane is subjected to uniformly distributed radial periodic loads at the internal surface and surrounded by a thermal field.A second-order nonlinear differential equation describing the radially symmetric motion of the membrane is ***,the dynamic characteristics of the system are qualitatively analyzed in terms of different material parameter spaces and ambient ***,for a given constant load,the bifurcation phenomenon of equilibrium points is *** is shown that there exists a critical load,and the phase orbits may be the asymmetrie homoclinic orbits of the“oo”***,for the system with two centers and one saddle point,the dynamic behaviors of the system show softening phenomena at both centers,but the temperature has opposite effects on the stiffness of the *** a given periodically perturbed load superposed on the constant term,some complex dynamic behaviors such as quasiperiodic and chaotic oscillations are *** the Poincare section and the maximum Lyapunov characteristic exponent,it is found that the ambient temperature could lead to the irregularity and unpredictability of the nonlinear system,and also changes the threshold of chaos.

关键词： Cylindrical membrane Radial perturbed load Ambient temperature Bifurcation and chaos

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Weak and smooth solutions to incompressible Navier-Stokes-Landau-Lifshitz-Maxwell equations

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Frontiers of Mathematics in China 2019年第6期14卷 1133-1161页

作者： Boling GUO fengxia LIU Institute of Applied Physics and Computational Mathematics Beijing 100088China The Graduate School of China Academy of Engineering Physics Beijing 100088China

Considering the Navier-Stokes-Landau-Lifshitz-Maxwell equations,in dimensions two and three,we use Galerkin method to prove the existence of weak *** combine the a priori estimates and induction technique,we obtain th... 详细信息

关键词： Weak solution smooth solution Navier-Stokes-Landau-Lifshitz-Maxwell equations

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In Situ Preparation of Au-SH@SO_(3)H-SBA-15 Catalyst with Enhanced Activity and Durability in Alkyne Hydration

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Chinese Journal of Chemistry 2014年第10期32卷 1072-1076页

作者： fengxia Zhu Hexing Li Jiangsu Key Laboratory for Chemistry of Low-Dimensional Materials School of Chemistry and Chemical EngineeringHuaiyin Normal UniversityHuaianJiangsu 223300China The Education Ministry Key Lab of Resource Chemistry Shanghai University of Electric PowerShanghai 200234China

A facile approach was developed for synthesizing Au-SH@SO_(3)H-SBA-15 with ordered mesoporous channels by reducing Au^(3+)to Au nanoparticles with SH-group bonded to silica support,followed by in situ coordinating Au with the unreacted *** catalyst exhibited high efficiency in alkyne hydration owing to the high activity of uniformly dispersed ultrasmall Au nanoparticles,the diminished diffusion limit due to the mesoporous structure,and the promoting effect of acidic SO_(3)H-groups resulting from oxidation of the SH-group by Au^(3+).Meanwhile,the catalyst could be easily recycled and displayed strong durability owing to the strong hydrothermal stability of mesoporous structure and the enhanced stability against Au leaching due to the Au-SH coordination bond.

关键词： gold(Au)nanoparticles hydration of alkynes water-medium clean reaction SH-functionalized SBA-15

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