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Identification and Analysis of SSRs Derived from Protein-coding Genes in Grape

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Agricultural Science & Technology 2017年第9期18卷 1579-1584页

作者： Pengfei WANG Ling SU Xilong JIANG Yingchun CHEN fengshan ren Yongmei WANG Shandong Engineering Research Center for Grape Cultivation and Deep-processing Shandong Academy of Grape

SSR(Simple Sequence Repeats), also known as microsatellites or STRs(short tandem repeats), are a type of PCRbased markers. So far, the version of grape genome has been updated constantly, but SSRs derived from protein-coding genes in grape have not yet been identified. In this study, 4 337 SSR-containing genes were found among 29 971 protein-coding genes in grape(Vitis vinifera L.), and 5 384 SSRs were found. There were 96 types of repeat motifs in SSRs derived from protein-coding genes in grape, and the most frequently occurring repeat motif was A/T. Among various repeat motifs in dinucleotide SSRs, the most frequently occurring repeat motif was AG/CT. Moreover, many genes exhibited codon usage bias, which was affected by the mutation pressure. GO annotation, KEGG annotation and domain analysis of these genes were *** genes were found to be closely related to the synthesis and metabolism of secondary metabolites, synthesis of flavones or anthocyanins, development and morphology of plant organs, and tolerance to biotic or abiotic stresses, including transcription factors in MYB, Hsf, NBS and TPC families. This study laid a solid foundation for the development of SSR markers and research of QTLs controlling complex agronomic traits in grape.

关键词： SSR Molecular markers Grape GO annotation Domain Transcription factor

Genome-wide Identification and Analysis of DNA Methyltransferases in Grape

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Agricultural Science & Technology 2017年第10期18卷 1781-1787,1794页

作者： fengshan ren Liying YANG Ling SU Lei GONG Pengfei WANG Yongmei WANG Shandong Engineering Research Center for Grape Cultivation and Deep-processing Shandong Academy of Grape Ji'nan 250100 China

DNA methylation is an important epigenetic regulation mechanism, which is catalyzed by DNA methyltransferases. In this study, eight DNA methyltransferase genes were identified in grape genome to analyze the selective pressure, gene expression and codon usage bias. The results showed grape DNA methyltransferase MET subfamily underwent relatively strong purifying selection during evolution, while chromomethylase CMT subfamily underwent positive selection during evolution. Under different abiotic(heat, drought or cold) stresses, the expression level of many grape DNA methyltransferase genes changed significantly. The expression level of these genes might be related with cis-regulatory elements of their promoters. The results of codon usage bias analysis showed that synonymous codon bias existed in grape DNA methyltransferase gene family, which might be affected by mutation pressure. These results laid a solid foundation for in-depth study of DNA methyltransferases in grape.

关键词： DNA methylation DNA methyltransferase Grape Selective pressure Codon usage bias

Advances in the Role of DNA Methylation and miRNAs in Plant Defense

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Agricultural Biotechnology 2017年第3期6卷 20-22页

作者： Pengfei WANG Xilong JIANG Xinying WU Ling SU fengshan ren Yongmei WANG Shandong Academy of Grape

DNA methylation and miRNAs are two important epigenetic molecular mechmxisms in plants. RNA-directed DNA methylation （RdDM） pathway is con- sidered as the most important mechanism of DNA methylation. A microRNA （miRNA） is processed from a 70-90-nucleotide-long single-stranded RNA precursor that forms a hairpin structure by Dicer enzyme, which plays an important role in plant development, regulation of plant gene expression and silencing of trans- posons. In recent years, a large number of studies have confirmed that DNA methylation and miRNAs play an important role in disease resistance mechanisms of plants. In this paper, recent progress on the role of DNA methylation and miRNAs in plant defense was summarized, which provided reference for revealing the re- lationship between disease resistance factors and epigenctic mechanisms in plants.

关键词： DNA methylation miRNAs Plant defense

Identification of Differentially Expressed Genes in Grape Skin at Veraison and Maturity and Construction of Co-expression Network

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Agricultural Science & Technology 2017年第11期18卷 1993-2000页

作者： Pengfei WANG Xilong JIANG Xinying WU Ling SU Lei GONG Hongmei SHI fengshan ren Yongmei WANG Shandong Engineering Research Center for Grape Cultivation and Deep-processing Shandong Academy of GrapeJi'nan 250100China Key Laboratory of Orban Agriculture in East China Ministry of Agriculture Ji'nan 250100 ChinaChina

The ripening process of grape is an important stage during grape growth and development. During this process, color of grape skin is the most obvious change. The molecular mechanism for the ripening of grape(a non-climacteric fruit, which ripens without ethylene and respiration bursts) is still unclear. Although numerous studies have been done on the changes in the contents of metabolites during grape ripening, the differentially expressed genes at veraison and maturity stages have not been systematically analyzed. In this study, 1 524 genes that are significantly differentially expressed in grape(Pinot Noir) skin at veraison and maturity stages were identified, and a co-expression network of these genes was built. Some of the eight co-expression modules we identified may be closely related to the synthesis or metabolism of anthocyanins, sugar acids, and other flavor substances. The transcription factor families WRKY, b ZIP, HSF and WOX may play an important role in the regulation of anthocyanin synthesis or metabolism. The results provide a foundation for further study of the molecular mechanism of grape ripening.

关键词： Grape Fruit ripening Co-expression network Co-expression module Anthocyanin Transcription factor

Research on the Necessity of Building National-level Grape and Cherry Germplasm Resources Bank in Shandong Province

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Agricultural Biotechnology 2018年第2期7卷 36-39页

作者： Pengfei WANG fengshan ren Yongmei WANG Xinying WU Xilong JIANG Yingchun CHEN Shan WANG Qian MU Liying YANG Wanjun CHEN Shandong Engineering Research Center for Grape Cultivation and Deep-processing Shandong Academy of GrapeJinan 250100China Key Laboratory of Urban Agriculture(East China) Ministry of AgricultureJinan 250100China

At present,the grape and cherry industries in Shandong are in the leading position in China and have driven the development of related high-end manufacturing industries such as wine,brandy,preserved fruit processing,fruit juice processing and health products. Therefore,vigorously developing the grape and cherry industries in Shandong Province and carrying out development and innovation are important parts of Shandong Province in responding to the strategy of national new and old kinetic energy conversion. However,currently the germplasm resources preserved in the fruit tree resources banks in China are only 45% of those in the US and 27. 2% of those in the EU. Moreover,the development of fruit trees resources banks in Shandong is relatively backward in China,and there is still no banks related with grape and cherry germplasm resources in Shandong. Therefore,importance can be attached to the agriculture,forestry,and animal husbandry to build germplasm resources banks for grape and cherry industries in Shandong Province. Building the national-level grape and cherry germplasm resources bank in Shandong Province can also promote the utilization of wild and farm germplasm resources in the future; advance the research on the genes related to disease resistance,stress resistance and quality of grapes and cherries; push forward the construction and development of cherry and grape mutants banks. It is conducive to the research on the agronomic traits of grapes and cherries,and can provide the parents resources for planting innovation and improving the quality of grapes and cherries,as well as promote the development and application of molecular markers of grapes and cherries,including the identification of lines and crossbreeding. Thereby,it cannot only promote the industry development,but also achieve the development of cultivation,breeding and basic research in an all-round way and the development of ＂ production,study and research＂ going side by side.

关键词： New and old kinetic energy conversion Middle-income trap Grape Cherry Germplasm resources bankHome

Skin and Internal Organ Involvement in Patients with Systemic Sclerosis

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Skin and Internal Organ Involvement in Patients with Systemi...

5种杀菌剂防治番茄茎腐病的室内筛选和田间使用效果

第六届中国中西医结合风湿病学术会议

作者： Sun You Zhang fengshan Zhao Yinhuan ren Luxue Pang Jinkui Department of Rheumatology, Second Hospital, Harbin Medical University, Harbin 150086, China

OBJECTIVE: The aim of this study was to evaluate the significant of skin and internal organ involvement in patients with systemic sclerosis (SSc) and to study the association between skin lesion and internal organ lesion. METHODS:Data were obtained from the Rheumatology Department of Harbin Medical University of 1993-2006. 112 patients were retrospected the clinical features of Rodnan Motified skin score,Furst organ score, initial symptom, age, disease duration, sex distribution, laboratory examination and serological *** association between internal organ involvement and these factors was evaluated by multiple factor regression analysis. RESULTS: Among the study group, 83 (74.1%) patients had Raynaud’s phenomenon as initial feature;the main deteriorated factor is inflammatory. Major organ complications include articulation and muscles (80.35%), gastrointestinal involvement (52.68%), heart involvement (21.4%), pulmonary disease(5.1%), renal involvement (4.1%). Internal organ involvement was more common in diffuse systemic sclerosis [dSSc] than limited systemic sclerosis [lSSc]. renal disease should be taken into account in diffuse systemic sclerosis which is more frequently impaired. Skin score is significantly correlated with organ score (r=3.38, P rence between two disease subtype in other factors. CONCLUSION: Systemic Sclerosis is a multisystemic disease characterized by sclerosis of the skin and visceral organs. But the patients present a great deal of variability in the extent of skin and internal organ involvement, the pace of the disease and consequently, the prognosis. Each patient must, therefore, be carefully investigated and be diagnosis earlier to improve the prognosis of disease.

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农学学报 2016年第5期6卷 18-22页

作者：任凤山高亮张博山东省农业科学院植物保护研究所/山东省植物病毒学重点实验室济南250100 山东农业大学植物保护学院山东泰安271018

笔者旨在通过筛选防治番茄茎腐病的有效药剂,为田间防治提供指导。室内测定了5种杀菌剂对终极腐霉菌丝生长的抑制作用,恶霉灵、唑醚·代森联、硫酸铜钙、甲基硫菌灵和恶酮·霜脲氰为有效杀菌剂。研究结果表明:恶霉灵的EC50为0.364 mg/L... 详细信息

笔者旨在通过筛选防治番茄茎腐病的有效药剂,为田间防治提供指导。室内测定了5种杀菌剂对终极腐霉菌丝生长的抑制作用,恶霉灵、唑醚·代森联、硫酸铜钙、甲基硫菌灵和恶酮·霜脲氰为有效杀菌剂。研究结果表明:恶霉灵的EC50为0.364 mg/L,抑菌效果最好;唑醚·代森联效果次之,EC50为9.247 mg/L,其他药剂防治效果相对较差。在田间药剂筛选试验中,70%恶霉灵可湿性粉剂与70%甲基硫菌灵可湿性粉剂组合防治效果为95.57%,均优于其它供试药剂组合,可应用于番茄茎腐病的田间防治。

关键词：番茄终极腐霉茎腐病杀菌剂化学防治

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系统性红斑狼疮免疫学检查的临床意义

哈尔滨医科大学学报 1987年第5期 36-39页

作者：张凤山任潞雪哈尔滨医科大学二院中医科

本文以作者临床实践为基础,对目前临床应用较有实用价值的免疫检查、LE细胞、ANA（抗核抗体）、anti-ds DNAab、anti Smab、LBT（皮肤免疫荧光带试验）等的临床意义进行评价,并结合文献复习,对免疫复合物、补体、细胞免疫异常等检测的... 详细信息

本文以作者临床实践为基础,对目前临床应用较有实用价值的免疫检查、LE细胞、ANA（抗核抗体）、anti-ds DNAab、anti Smab、LBT（皮肤免疫荧光带试验）等的临床意义进行评价,并结合文献复习,对免疫复合物、补体、细胞免疫异常等检测的临床意义作了初步讨论。强调了ANA检查的高敏感性及抗ds DNA抗体、抗Sm抗体、非暴光“正常”皮肤LBT检查对本病早期诊断的高特异性。

关键词：系统性红斑狼疮免疫学检查

蓝紫色月季品种“紫霞仙子”花发育阶段转录组分析

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山东农业科学 2024年第11期56卷 13-20页

作者：孙骏飞孟宪水张晶莹任凤山李益山东省农业科学院休闲农业研究所/农业农村部华东都市农业重点实验室/生态园艺植物育种山东省工程研究中心山东济南250100 平阴县玫瑰研究所山东平阴250407

以蓝紫色月季“紫霞仙子”(Rosa‘Nightingale’)花发育5个时期的花瓣为材料,基于转录组分析,从分子水平上探索其花发育期花色相关基因的表达模式。结果表明,初开期相比花蕾期(N_S2 vs N_S1)、半开期相比初开期(N_S3 vs N_S2)、盛开期... 详细信息

以蓝紫色月季“紫霞仙子”(Rosa‘Nightingale’)花发育5个时期的花瓣为材料,基于转录组分析,从分子水平上探索其花发育期花色相关基因的表达模式。结果表明,初开期相比花蕾期(N_S2 vs N_S1)、半开期相比初开期(N_S3 vs N_S2)、盛开期相比半开期(N_S4 vs N_S3)、落花期相比盛开期(N_S5 vs N_S4)分别有386、1492、830、255个上调表达的差异基因和607、1849、150、783个下调表达的差异基因。在类黄酮与花青素的合成通路中共找到差异表达基因15个,类黄酮与花青素合成在半开期到盛开期达到顶峰;R2R3MYB转录因子MYB6和MYB113与类黄酮、花青素合成相关基因的表达趋势相同,可能参与了花青素合成的调控。在类胡萝卜素合成通路中共找到了14个差异表达基因,类胡萝卜素的合成在初开期到半开期达到顶峰。可见,类胡萝卜素的合成较花青素提前,因此可避免黄色对“紫霞仙子”的蓝紫色花色造成干扰。

关键词： “紫霞仙子”月季蓝紫色花色转录组分析类黄酮花青素类胡萝卜素