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Wheat peptides with different hydrolysis degree have similar relief effect in constipated mice

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Food Bioscience 2023年第2期52卷 879-885页

作者： Qianqian Wang Fei Shen Junhui Zhang Jiachen Zhuang fengqin feng College of Biosystems Engineering and Food Science Zhejiang UniversityHangzhou310058China National Engineering Laboratory of Intelligent Food Technology and Equipment Zhejiang UniversityHangzhou310058China Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture Zhejiang UniversityHangzhou310058China

Constipation has become more common in recent decades,affecting people’s quality of *** bioactive substances could be effective in alleviating *** goal of this study was to assess and compare the efficacy of low and high hydrolysis degree wheat peptides(WP-L and WP-H)at a dose of 1 mg/*** on relieving loperamide-induced constipation in mice and the underlying ***,we proved that both WP-L and WP-H treatment showed constipation relieving effect by improving defecation function and small intestinal propulsion rate while the different hydrolysis degree did not affect the efficacy of ***,WP-L and WP-H could balance the secretion of excitatory and inhibitory factors,and enhance the activity of antioxidant enzyme(SOD and GSH-Px).Importantly,both of them could accelerate intestinal motility,improve water-salt metabolism and intestinal barrier,and ameliorate oxidative *** summary,our findings indicated that 1 mg/*** WP-L and WP-H effectively relieved loperamide-induced constipation by affecting multiple targets in mice.

关键词： Wheat peptides Hydrolysis degree Constipation Intestinal barrier Oxidative stress

Synthesis and characterization of medium- and long-chain structural lipid rich in α-linolenic acid and lauric acid

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Food Bioscience 2023年第2期52卷 203-214页

作者： Ying Huang Wangxin Liu Xianliang Luo Minjie Zhao Tao Liu fengqin feng College of Biosystems Engineering and Food Science Zhejiang UniversityHangzhou310058China ZhongYuan Institute Zhejiang UniversityZhengzhou450001China College of Food Science and Engineering Tarim UniversityAlarXinjiang843300China Production&Construction Group Key Laboratory of Special Agricultural Products Further Processing in Southern Xinjiang AlarXinjiang843300China

Medium-and long-chain triglycerides(MLCT)have been widely *** this study,lauric acid(La)was incorporated into flaxseed(Linum usitatissimum L.)oil(FO,aLna-riched)by lipase-catalyzed *** synthesis efficiency of four immobilized lipases(Lipozyme RM IM,TL IM,AOAB8 and Novozym 435)was compared,while Lipozyme AOAB8 reaction for 2 h was the best ***,the optimal conditions for synthesizing structural lipids(aLSLs,rich in aLna and La)were obtained by response surface methodology:the reaction temperature was 44.6℃,substrate molar ratio was 12(La/FO),and enzyme loading was 9.11%.After purification by molecular distillation,the triglycerides(TAGs)of aLSLs contained 75.35% of TAGs withα-linolenic acid(aLna),40.82%of MLCTs with La,and 26.75%(65.53% of the total MLCTs)of TAGs with both aLna and La(ɑ-MLCT).Meanwhile,the content of La and aLna in aLSLs were 22.38%and 39.78%,*** chemical structure and microstructure of aLSLs were similar to *** addition,the thermo-oxidative decomposition behavior of aLSLs was significantly improved compared with physical mixture *** improvement might be related to the insertion of La and the structure of *** all,this study firstly developed a novel thermo-oxidatively stable MLCT with high levels of aLna and La.

关键词： Structural lipids Medium-and long-chain triglycerides Physicochemical properties Lauric acid α-Linolenic acid

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酪蛋白源肽TPTLN助睡眠作用及其机制

食品科学 2025年第3期46卷 119-127页

作者：邓世洁刘玲黄菊冯凤琴赵敏洁杜鹃浙江海洋大学食品与药学学院浙江大学生物系统工程与食品科学学院杭州康源食品科技有限公司

目的：明确酪蛋白源肽TPTLN的助睡眠作用并探究其可能的作用机制。方法：将30只小鼠平均分成3组，实验组灌胃酪蛋白源肽TPTLN（剂量为5 mg/kg mb），阳性组腹腔注射地西泮（剂量为1 mg/kg mb），对照组灌胃灭菌水（剂量为5 mg/kg mb）... 详细信息

目的：明确酪蛋白源肽TPTLN的助睡眠作用并探究其可能的作用机制。方法：将30只小鼠平均分成3组，实验组灌胃酪蛋白源肽TPTLN（剂量为5 mg/kg mb），阳性组腹腔注射地西泮（剂量为1 mg/kg mb），对照组灌胃灭菌水（剂量为5 mg/kg mb），所有实验小鼠每日称质量，连续给药7 d后进行戊巴比妥钠诱导睡眠行为学实验，评估睡眠质量，再继续给药4 d后进行解剖取样；通过酶联免疫吸附试验测定小鼠血清和脑组织中5-羟色胺（5-hydroxytryptamine,5-HT）、γ-氨基丁酸（γ-aminobutyric acid,GABA）、5-羟基吲哚乙酸（5-hydroxyindoleacetic acid,5-HIAA）、谷氨酸（glutamate,Glu）、β-内啡肽（β-endorphin,β-EP）、皮质醇、去甲肾上腺素（noradrenaline,NE）的质量浓度以及对GABA/Glu值进行评估；实时荧光定量聚合酶链式反应分析TPTLN对下丘脑中γ-氨基丁酸A型受体α1亚单位（γ-aminobutyric acid A receptor α1,GABAA-α1）和5-羟色胺受体1A（5-hydroxytryptamine receptor 1A,5-HT1A）mRNA相对表达水平的影响。结果：相比于对照组，TPTLN在戊巴比妥钠的协同下可以提高小鼠的入睡率至100%，睡眠潜伏期显著缩短31.7%（P<0.05），睡眠时长延长1.1倍（P<0.05）；血清和脑组织中GABA/Glu的比值分别提高了68.8%和19.3%，明显改善了GABA/Glu抑制/兴奋代谢失衡（P<0.001,P<0.000 1），血清中GABA的质量浓度高度显著提高了49.4%（P<0.001），脑组织中5-HT的水平上升了7.6%，脑组织中皮质醇、NE的含量分别显著降低了24.3%和23.4%（P<0.05）；显著上调了GABAA-α1和5-HT1A基因表达水平（P<0.01、P<0.05），分别是对照组的1.5倍和1.3倍。结论：酪蛋白源肽TPTLN具有良好的助睡眠作用，其机制可能与调控GABAA-α1、5-HT1A的水平及脑内神经递质GABA、Glu、5-HT、皮质醇、NE的含量有关。

关键词：酪蛋白肽助睡眠神经递质作用机制

Evaluation of dynamic effects of dietary medium-chain monoglycerides on performance,intestinal development and gut microbiota of broilers in large-scale production

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Animal Nutrition 2023年第3期14卷 269-280页

作者： Tao Liu Shengyue Ruan Qiufen Mo Minjie Zhao Jing Wang Zhangying Ye Li Chen fengqin feng College of Biosystems Engineering and Food Science Zhejiang UniversityHangzhou 310058China The Institute of Animal Husbandry and Veterinary Science Zhejiang Academy of Agricultural SciencesHangzhou 310021China Ningbo Research Institute Zhejiang UniversityNingbo 315000China Hangzhou Longyu Biotechnology Co. LtdHangzhou 310003China

Medium-chain monoglycerides(MG)have been reported to affect the productive performance,gut microbiota and health of broiler chickens reared in ideal experimental conditions at home and ***,the effects of MG on performance,intestinal development and gut microbiota of chickens in large-scale farms during different feed stages remain *** present study was conducted on a modern farm with a total of 12,000 yellow feathered broiler chicks that were randomly allotted to 2groups(1000 chicks/replicate,6 replicates/group)for a 70-day *** control group(CON group)received a basal diet,and the treated group(MG group)was fed a basal diet containing 300 mg/kg mixed *** results revealed that dietary MG significantly(P<0.05)increased the body weight and average feed intake,but notably reduced the feed conversion and mortality of chickens in large-scale production during the starter *** villus height of the duodenum in the MG group at 1,2 and 7 wk of age increased notably,and the villus height to crypt depth ratio at 1,2,5 and 10 wk of age was *** MG decreased the serum insulin content of chickens at 5,7 and 10 wk of age,and decreased the serum lipopolysaccharide at 3 and 7 wk of *** triglyceride level of chickens at 3,5 and 10 wk of age and the low-density lipoprotein cholesterol level of chickens at 7 and 10 wk of age in the MG group decreased notably,while the high-density lipoprotein cholesterol increased ***,MG supplementation selectively increased the relative abundance of genus Bacteroides(family Bacteroidaceae)and Lachnospiraceae_NK4A136_group,but decreased the content of genus Rikenellaceae_RC9_gut_group,Collinsella and family Barnesiellaceae in the cecum of chickens at 3,7 and 10 wk of ***,these findings showed that dietary MG notably enhanced chicken performance,health and feed nutrient utilization at early ages by regulating gut microbiota,intestinal development and serum biochemical indice

关键词： Medium-chain monoglycerides Broiler chickens Dynamic effect Gut microbiota Fat deposition

Dietary glycerol monolaurate improves bone growth through the regulation of IGF-1 in the fish model

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Food Bioscience 2022年第6期50卷 1540-1548页

作者： Yan Chen Yong Rui Yuchao Wang Minjie Zhao Tao Liu Jiachen Zhuang fengqin feng College of Biosystems Engineering and Food Science Zhejiang UniversityHangzhou310058China Mizuda Group Co. Ltd/Hangzhou Xinchao Enterprise Management Consulting Co.LtdHuzhou313000China Hangzhou Longyu Biotechnology Co. LtdHangzhou310003China Ningbo Research Institute Zhejiang UniversityNingbo15100China

Glycerol monolaurate (GML) has the potential of antibacterial and growth-promoting in food. Recent research reported that GML could significantly increase the body index of fish, but whether GML can exert growth-promoting ability via regulating bone metabolism remains unknown. This study explored the effects of GML on the bone growth of fish models and its mechanisms by analyzing the expression of insulin-like growth factor 1(IGF-1). The micro-CT scanning results showed that the dietary supplementation of 750 mg/kg GML significantly increased the body length and bone mineral density, and improved the bone microarchitecture parameters of the caudal vertebrae of zebrafish (Danio rerio ). The centrum part of the vertebra was easier to respond to the regulatory effect of GML than the neural arch and the haemal arch. Mechanistically, the content of serum IGF-1, the expression of osteogenic differentiation-related genes, and genes involved in IGF-1/PI3K/Akt signaling pathway were significantly increased. The liver proteomic analysis showed the consistent results that GML supplementation could up-regulate the expression of hepatic IGF-1, and that energy metabolism and growth and development-related metabolic pathways were implicated in the GML bone growth boosting effects. These findings highlight that GML could be used as a functional food additive to improve bone growth and health.

关键词： Glycerol monolaurate Bone growth Insulin-like growth factor 1 Danio rerio Proteomic analysis

Screening of novel probiotics with intestinal peristalsis-promoting potential based on in vitro and in vivo investigations

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FOOD BIOSCIENCE 2023年第3期53卷 2013-2022页

作者： Shen, Fei Zhuang, Jiachen Wang, Qianqian Zhang, Junhui Liu, Tao Ruan, Shenyue Du, Juan Zhong, Hao Zhao, Mingjie feng, fengqin Zhejiang Univ Coll Biosyst Engn & Food Sci Natl Local Joint Engn Lab Intelligent Food Technol Zhejiang Key Lab Agrofood Proc Hangzhou 310058 Peoples R China Zhejiang Univ Ningbo Res Inst Ningbo 315100 Peoples R China Zhejiang Univ ZhongYuan Inst Zhengzhou 450001 Peoples R China Hangzhou Kangyuan Food Sci & Technol Co Ltd Hangzhou 310012 Peoples R China Zhejiang Univ Technol Coll Food Sci & Technol Hangzhou 310014 Peoples R China

Constipation is a widespread gastrointestinal issue. This work aimed to investigate anti-constipation capacities of 9 novel strains of Lactobacillus and Bifidobacterium using in vitro experiments for initial exploration and then further confirmed through in vivo models including both zebrafish and mice. The in vitro experimental results revealed that L. plantarum T34 and L. paracasei YJ10 could survive under the simulated gastrointestinal tract conditions as standard strain L. rhamnosus (LGG) did. L. paracasei YJ10 and L. casei Lc showed the highest 22 h's aggregation rate. L. rhamnosus W1 showed the highest coaggregation rate with E. coli and with S. aureus. T34 had the largest inhibition zones against S. typhimurium and L. Monocytogenes. The highest adhesion rates with HT-29 cells were L. casei Lc, LGG and W1. Principal component analysis (PCA) and Fuzzy synthetic evaluation (FSE) were performed to evaluate the in vitro probiotic potential of the 9 strains comprehensively. The order of scores on in vitro probiotic property from high to low was as follows: T34, W1, YJ10, Lc, LGG, SLT, SS, Lb, YN4 and LQSQ. The subsequent in vivo experiment of zebrafish further confirmed T34, W1 and YJ10 have better anticonstipation ability compared to other strains. Furthermore, T34 relieve loperamide-induced constipation by promoting gut transit in the mice model. In conclusion, T34 was demonstrated to prevent constipation and may be suggested for use in food and pharmaceutical goods.

关键词： Probiotic screening Lactobacillus Constipation in vitro Zebrafish

海参肽的抗氧化稳定性及对细胞氧化损伤的保护作用

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中国食品学报 2023年第10期23卷 23-31页

作者：王倩倩杜鹃冯凤琴浙江大学生物系统工程与食品科学学院杭州310058 杭州康源食品科技有限公司杭州310003

目的:探究不同水解度海参肽(低水解度为SCP-L和高水解度为SCP-H)的抗氧化稳定性及对H_(2)O_(2)诱导的L929细胞氧化应激损伤的保护作用。方法:首先以羟自由基清除率(·OH)为评价指标,考察温度、pH、食品配料、金属离子和模拟胃肠环境对... 详细信息

目的:探究不同水解度海参肽(低水解度为SCP-L和高水解度为SCP-H)的抗氧化稳定性及对H_(2)O_(2)诱导的L929细胞氧化应激损伤的保护作用。方法:首先以羟自由基清除率(·OH)为评价指标,考察温度、pH、食品配料、金属离子和模拟胃肠环境对海参肽抗氧化稳定性的影响。然后,利用H_(2)O_(2)诱导L929细胞建立氧化应激损伤模型,测定细胞的存活率及细胞水平的抗氧化指标。结果:在20~100℃范围,SCP-L和SCP-H均具有良好的热稳定性;在氯化钠、Cu^(2+)、Zn^(2+)、碱性和模拟胃液环境下降低了其抗氧化稳定性;模拟胃肠消化,提高了SCP-L和SCP-H的抗氧化活性。SCP-L和SCP-H质量浓度在0.2~0.8 mg/mL时,显著提高了氧化损伤L929细胞的存活率;与模型组相比,SCP-L和SCP-H质量浓度为0.6 mg/mL时,乳酸脱氢酶(LDH)活力分别显著下降了20.67%和25.91%,丙二醛(MDA)含量分别显著下降了26.39%和44.36%,超氧化物歧化酶(SOD)活力分别显著提高了89.63%和130.17%,谷胱甘肽过氧化物酶(GSH-Px)活力分别显著提高了26.22%和40.41%。结论:海参肽在储存过程中应尽量避免与碱性、含氯化钠、Cu^(2+)和Zn^(2+)等环境因素接触。SCP-L和SCP-H均能有效阻止H_(2)O_(2)诱导的L929细胞氧化应激损伤,且SCP-H效果优于SCP-L。

关键词：海参肽水解度稳定性氧化损伤

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海参肽对大鼠乙酸性胃溃疡愈合的促进作用

中国食品学报 2022年第8期22卷 109-117页

作者：王倩倩杜鹃冯凤琴浙江大学生物系统工程与食品科学学院杭州310058 浙江诺衍生物科技有限公司浙江湖州313000

通过建立大鼠切除型皮肤伤口模型,测定伤口的愈合率,观察海参肽是否能够促进大鼠伤口愈合。建立乙酸诱导的大鼠胃溃疡模型,并以不同剂量的海参肽(0.5 mg/g和2 mg/g)灌胃干预,以氧化应激、炎症因子和防御因子相关生化指标来评价其对大鼠... 详细信息

通过建立大鼠切除型皮肤伤口模型,测定伤口的愈合率,观察海参肽是否能够促进大鼠伤口愈合。建立乙酸诱导的大鼠胃溃疡模型,并以不同剂量的海参肽(0.5 mg/g和2 mg/g)灌胃干预,以氧化应激、炎症因子和防御因子相关生化指标来评价其对大鼠胃溃疡的愈合是否有促进作用以及可能的机制。结果表明:涂抹海参肽的大鼠伤口愈合率自造模后第2天起显著高于对照组,第12天时完全愈合。与模型组相比,海参肽2个剂量组的溃疡面积均显著降低,溃疡抑制率分别达41.45%和60.41%;形态学结果显示黏膜再生、炎性细胞浸润减少,预后良好;胃组织中抗氧化相关的超氧化物歧化酶(SOD)活力和谷胱甘肽过氧化物酶(GSH-Px)活力分别增加3.80%,4.85%和12.59%,23.54%,丙二醛(MDA)含量分别降低了59.40%,45.24%;炎症相关的肿瘤坏死因子-α(TNF-α)和白介素-6(IL-6)水平分别降低12.37%,19.63%和8.64%,23.43%;防御因子相关的前列腺素E2(PGE2)、一氧化氮(NO)和表皮生长因子(EGF)水平分别增加12.80%,16.95%;14.06%,27.44%和25.08%,63.76%。海参肽通过增强胃黏膜的抗氧化、抗炎和防御能力等途径发挥促进胃溃疡的愈合作用。

关键词：海参肽乙酸胃溃疡愈合

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月桂酸单甘油酯抑菌机理、影响因素及其复配体系的抑菌特性

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中国食品学报 2016年第3期16卷 146-151页

作者：蒋增良张辉杜鹃冯凤琴浙江大学生物系统工程与食品科学学院馥莉食品研究院浙江省农产品加工技术研究重点实验室浙江省食品加工技术与装备工程研究中心杭州310058 杭州康源食品科技有限公司杭州310000

月桂酸单甘油酯(Glycerol monolaurate,简称GML)是一种亲酯性非离子型表面活性剂,同时也是一种安全、高效、广谱的抗菌剂,天然存在于母乳、椰子油和美洲蒲葵中,具有乳化和抑菌双重功能。本文综述月桂酸单甘油酯的抑菌机理,食品组分、水... 详细信息

月桂酸单甘油酯(Glycerol monolaurate,简称GML)是一种亲酯性非离子型表面活性剂,同时也是一种安全、高效、广谱的抗菌剂,天然存在于母乳、椰子油和美洲蒲葵中,具有乳化和抑菌双重功能。本文综述月桂酸单甘油酯的抑菌机理,食品组分、水溶性及体系条件(温度、p H、盐度)对月桂酸单甘油酯抑菌活性的影响,并分析了与其他常见食品防腐剂复配体系的抑菌特性,阐述月桂酸单甘油酯在食品工业中应用的潜力和重要意义,以期为GML更合理的使用提供指导。

关键词：月桂酸单甘油酯抑菌机理食品组分影响因素复配体系