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xiao-Ping Chen-A leading figure among Chinese surgeons

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Science China(Life Sciences) 2016年第10期59卷 993-994页

作者： Chen xiao-Ping

xiao-Ping Chen,male,born in Funan County of China’s eastern Anhui Province in 1953,was considered a reformer and innovator in surgery of the liver,pancreas and biliary *** four decades of research and clinical work,he has made remarkable achievements in regards to the treatment of hepato-pancreato-biliary diseases and has been elected as a member of the Chinese Academy of Sciences in2015 because of his outstanding contributions.

关键词：中国东部外科中国科学院院士医生人物阜南县安徽省肝脏

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Modular assembly of complex C-alkyl glycoside enabled via cooperative NHC and photoredox catalysis

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Science China Chemistry 2025年

作者： Rui-Qiang Jiao Ming Li Xi Chen Mei-Ze Xu Tian Ding xiao-Ping Gong Zhuo-Mei Li Xue-Yuan Liu Yong-Min Liang State Key Laboratory of Applied Organic Chemistry Lanzhou University College of Chemistry and Chemical Engineering Northwest Normal University

C-Alkyl glycosides are found in natural products and are indispensable in drug development programs,owing to their diverse range of medicinal ***-component reactions involving glycosyl radical addition are well documented and proved efficient for the C-alkyl glycosides ***,multicomponent reactions for the rapid and controllable construction of structurally diversified C-alkyl glycosides in a single step remains a significant challenge due to the issues associated with balancing reactivity,compatibility,and ***,we report an NHC and photoredox co-catalyzed three-component method to access complex and valuable C-alkyl *** protocol exhibits broad substrate scope and excellent chemoand ***,nine types of sugars are compatible with this *** addition,the generality of this catalytic strategy was further highlighted by the potential for scalable production,the feasibility of further modifications of the products and its successful application in the late-stage functionalization of pharmaceutical skeletons.

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miR-483-3p增强胰腺癌对吉西他滨敏感性及分子机制研究

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大连理工大学学报 2025年第1期65卷 30-37页

作者：刘青肖桂山大连理工大学化工学院辽宁大连116024

吉西他滨耐药限制了其对胰腺癌的治疗效果,而传统化学药物无法有效克服吉西他滨对胰腺癌的耐药.研究表明,microRNA(miRNA)的异常表达与肿瘤发生、发展和化疗耐药性有关.对miR-483-3p在胰腺癌吉西他滨耐药中的作用和潜在机制进行了研究.q... 详细信息

吉西他滨耐药限制了其对胰腺癌的治疗效果,而传统化学药物无法有效克服吉西他滨对胰腺癌的耐药.研究表明,microRNA(miRNA)的异常表达与肿瘤发生、发展和化疗耐药性有关.对miR-483-3p在胰腺癌吉西他滨耐药中的作用和潜在机制进行了研究.qPCR结果显示miR-483-3p在胰腺癌耐药细胞中显著下调.miR-483-3p minics瞬时转染显著提高了耐药细胞内miR-483-3p的表达水平,显著减弱了耐药细胞的增殖、迁移、转移和侵袭能力,恢复了吉西他滨的反应性.Western blot结果显示miR-483-3p抑制PI3K和p-AKT蛋白的表达水平,抑制Bcl2蛋白表达,促进Bax、Bad和cleaved-Caspase-3蛋白表达,促进细胞凋亡进程.总之,研究结果表明miR-483-3p通过抑制PI3K/AKT信号通路促进细胞凋亡,增加胰腺癌对吉西他滨的敏感性,miR-483-3p可能是吉西他滨耐药胰腺癌患者的潜在治疗策略.

关键词： miR-483-3p 胰腺癌吉西他滨耐药性 miRNA

Influence of sex on outcomes of liver transplantation for hepatocellular carcinoma:a multicenter cohort study in China

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Cancer Biology & Medicine 2024年第4期21卷 347-362页

作者： Jian Chen Zhe Yang fengqiang Gao Zhisheng Zhou Junli Chen Di Lu Kai Wang Meihua Sui Zhengxin Wang Wenzhi Guo Guoyue Lyu Haizhi Qi Jinzhen Cai Jiayin Yang Shusen Zheng xiao Xu Zhejiang University School of Medicine Hangzhou 310030China Institute of Organ Transplantation Zhejiang UniversityHangzhou 310030China Department of Hepatobiliary and Pancreatic Surgery Shulan Hospital of HangzhouHangzhou 310006China National Center for Healthcare Quality Management in Liver Transplant Hangzhou 310003China Department of General Surgery Huashan HospitalFudan UniversityShanghai 200040China Department of Hepatobiliary and Pancreatic Surgery The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China Department of Hepatobiliary and Pancreatic Surgery The First Hospital of Jilin UniversityChangchun 130021China Department of Liver Transplantation Second Xiangya HospitalCentral South UniversityChangsha 410008China Department of Organ Transplantation Center The Affiliated Hospital of Qingdao UniversityQingdao 266003China Department of Liver Surgery and Liver Transplantation Center West China HospitalSichuan UniversityChengdu 610044China NHC Key Laboratory of Combined Multi-organ Transplantation Hangzhou 310003China Department of Hepatobiliary and Pancreatic Surgery The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China

Objective:Sex-specific differences are observed in various liver diseases,but the influence of sex on the outcomes of hepatocellular carcinoma(HCC)after liver transplantation(LT)remains to be *** study is the first Chinese nationwide investigation of the role of sex in post-LT outcomes in patients with ***:Data for recipients with HCC registered in the China Liver Transplant Registry between January 2015 and December 2020 were *** associations between donor,recipient,or donor-recipient transplant patterns by sex and the post-LT outcomes were studied with propensity score matching(PSM).The survival associated with different sex-based donor-recipient transplant patterns was further ***:Among 3,769 patients enrolled in this study,the 1-,3-,and 5-year overall survival(OS)rates of patients with HCC after LT were 96.1%,86.4%,and 78.5%,respectively,in female recipients,and 95.8%,79.0%,and 70.7%,respectively,in male recipients after PSM(P=0.009).However,the OS was comparable between recipients with female donors and male *** analysis indicated that male recipient sex was a risk factor for post-LT survival(HR=1.381,P=0.046).Among the donor-recipient transplant patterns,the male-male donor-recipient transplant pattern was associated with the poorest post-LT survival(P<0.05).Conclusions:Our findings highlighted that the post-LT outcomes of female recipients were significantly superior to those of male recipients,and the male-male donor-recipient transplant pattern was associated with the poorest post-LT *** from male donors may provide the most benefit to female *** results indicate that sex should be considered as a critical factor in organ allocation.

关键词： Sex liver transplantation hepatocellular carcinoma outcome recipient donor

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毛竹PheTCP19b基因可变剪接体对芽发育的影响

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农业生物技术学报 2025年第1期33卷 43-54页

作者：郝琴申孜闫小玲周敏舒郭小勤浙江农林大学竹子研究院/省部共建亚热带森林培育国家重点实验室

可变剪接(alternative splicing, AS)是一种转录后的修饰过程，在生物体各种生理过程中发挥重要作用。TCP是一种植物特有的转录因子，参与植物多种生长发育调控过程。为深入探究毛竹(Phyllostachys edulis) TCP19b基因可变剪接体的表达... 详细信息

可变剪接(alternative splicing, AS)是一种转录后的修饰过程，在生物体各种生理过程中发挥重要作用。TCP是一种植物特有的转录因子，参与植物多种生长发育调控过程。为深入探究毛竹(Phyllostachys edulis) TCP19b基因可变剪接体的表达特性及生物学功能，本研究采用RT-PCR技术克隆了毛竹TCP19b基因全长CDS，采用qRT-PCR技术和GUS组织染色分析了可变剪接体的表达量及表达部位，通过亚细胞定位及遗传转化分析了可变剪接体编码蛋白的定位及生物学功能。结果显示，毛竹PheTCP19b基因发生了可变剪接，产生了2种可变剪接体PheTCP19bα和PheTCP19bβ，分别编码346和382个氨基酸，2种可变剪接体都包含保守的bHLH结构域。PheTCP19b与水稻(Oryza sativa) OsTCP19亲缘关系最近。2个可变剪接体的组织特异性表达模式存在一定差异，PheTCP19bα的组织特异性强，而PheTCP19bβ表现出组成型表达特性，油菜素内酯(brassinolide, BR)可诱导2个可变剪接体的表达。2个可变剪接体蛋白均富集在细胞核。PheTCP19bα和PheTCP19bβ均可以将拟南芥(Arabidopsis thaliana) brc1突变体多分枝表型回补到野生型表型。本研究为进一步探讨PheTCP19b基因不同剪接体在芽发育过程中的分子机制提供了基础资料。

关键词：毛竹 TCP转录因子可变剪接(AS) 芽发育油菜素内酯(BR)

Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu Fu Lei Gu Xin Shao Qinqin Wang Xin Dong Bin Duan Feiyang Xing Jun Wu Minmin xiao Fangqing Zhao Jing-Dong J.Han Qi Liu xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

[Progress in applications of ambient ionization mass spectrometry for lipids identification]

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Se pu = Chinese journal of chromatography 2025年第1期43卷 22-32页

作者： xiao-Rong Wang Yi-Yan Yin Jin Ouyang Na Na Key Laboratory of Radiopharmaceuticals Ministry of Education College of Chemistry Beijing Normal University Beijing 100875 China. Department of Chemistry College of Arts and Sciences Beijing Normal University Zhuhai 519087 China.

Lipids are indispensable components of living organisms and play pivotal roles in cell-membrane fluidity, energy provision, and neurotransmitter transmission and transport. Lipids can act as potential biomarkers of diseases given their abilities to indicate cell-growth status. For example, the lipid-metabolism processes of cancer cells are distinct from those of normal cells owing to their rapid proliferation and adaptation to ever-changing biological environments. As a result, the ability to rapidly detect, identify, and monitor lipid components is critical for tracking life-related processes and may enhance cancer diagnosis and treatment efficacy. Mass spectrometry (MS) is regarded to be among the most efficient methods for directly obtaining molecular-structural information, and is distinctly advantageous for identifying lipids. Recent years have witnessed the emergence of ambient mass spectrometry (AMS), which enables direct analyte sampling and ionization without the need for sample preprocessing. These characteristics endow AMS with special advantages for identifying and monitoring lipids. Furthermore, the ongoing development of soft ionization technologies has led to the widespread use of AMS for the detection of complex and diverse lipid molecules. Electrospray ionization (ESI) is a gentle ionization method that can be used to detect medium-to-high-polarity compounds and provide detailed chemical information for lipids by producing a fine mist of charged droplets from a liquid sample. Consequently, a series of ESI-based ionization methods have been developed for fabricating different AMS systems capable of rapidly detecting lipids in a simple manner. For example, desorption electrospray ionization (DESI) is among the most extensively employed ambient ionization techniques, and has been used to detect a wide range of samples, including solids, liquids, and gases. DESI involves spraying a charged solvent onto the surface of a sample, after which the solvent is

关键词： ambient mass spectrometry (AMS) ionization techniques lipid lipid fine structure tandem mass spectrometry (MS/MS)

基于颜色校正与改进的对比度增强的水下图像增强方法

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现代电子技术 2025年第1期48卷 40-46页

作者：刘明肖汉城云南民族大学电气信息工程学院云南昆明650500

针对水下介质分布不均匀引起的光吸收和散射导致图像颜色失真、对比度低、细节模糊,严重影响ORBSLAM2算法前端特征提取与匹配鲁棒性的问题,文中提出一种基于颜色校正与改进的自适应对比度增强的图像处理方法。首先,利用一种改进的颜色... 详细信息

针对水下介质分布不均匀引起的光吸收和散射导致图像颜色失真、对比度低、细节模糊,严重影响ORBSLAM2算法前端特征提取与匹配鲁棒性的问题,文中提出一种基于颜色校正与改进的自适应对比度增强的图像处理方法。首先,利用一种改进的颜色通道补偿和颜色通道拉伸方法去除色偏;其次,采用改进的自适应对比度增强方法提高图像的亮度与对比度;最后,将彩色校正图像与对比度增强图像在HSV空间中融合。此外,将提出的算法与一些其他算法进行主客观的评价,并将处理好的图片进行特征提取和匹配。结果表明,该算法处理的图片不仅在主客观评价上均优于对比算法,而且增加了特征点的匹配数量,为水下图像处理提供了借鉴。

关键词：水下视觉SLAM 水下图像增强自适应对比度增强颜色校正颜色补偿特征匹配

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环境分权对区域工业绿色发展影响的空间溢出效应研究

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生态经济 2025年第1期41卷 201-209,229页

作者：王晗阚萧江苏师范大学公共管理与社会学院江苏徐州221116

在理论机制分析的基础上,基于2005—2020年我国30个省份的面板数据,采用Super-SBM模型测度了区域工业绿色发展水平,进而采用空间计量模型等方法检验了环境分权对区域工业绿色发展的影响。研究发现:(1)在考虑空间溢出效应的前提下,环境... 详细信息

在理论机制分析的基础上,基于2005—2020年我国30个省份的面板数据,采用Super-SBM模型测度了区域工业绿色发展水平,进而采用空间计量模型等方法检验了环境分权对区域工业绿色发展的影响。研究发现:(1)在考虑空间溢出效应的前提下,环境分权对区域工业绿色发展具有显著的抑制作用,但空间影响程度较为有限。使用工具变量处理内生性问题后,上述核心结论依然成立。(2)环境行政分权、环境监察分权及环境监测分权对工业绿色发展在影响方向或强度上存在显著差异。(3)进一步分析表明,财政分权、地方政府竞争程度的提升,强化了环境分权对工业绿色发展的抑制作用。(4)相比于东部地区,环境分权及其细分指标在中部和西部地区的边际效应更强;相比于2005—2012年,2013—2020年环境分权及细分指标的边际效应有所减弱。因此,建议建立跨级环境治理协作机制,强化数据透明度和公信力,明确权责匹配原则以及在环保垂管体制下细化环境分权政策。

关键词：环境分权工业绿色发展空间溢出效应区域异质性

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