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Analysis of non-coding small RNA similarity/sharing between plant and animal genomes

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Science China(Life Sciences) 2023年第10期66卷 2437-2440页

作者： fengming huang Xiangyu Qiao Song Mei Cong Zhang Chengyu Jiang State Key Laboratory of Common Mechanism Research for Major Diseases Department of BiochemistryInstitute of Basic Medical Sciences Chinese Academy of Medical SciencesSchool of Basic Medicine Peking Union Medical CollegeBeijing 100005China

Dear Editor,During the construction and analysis of the Bencao(herbal)small RNA(s RNA)Atlas,the fact that many s RNAs from the human genome are present in the Bencao(herbal)database attracted our attention(Cao et al.,2023).The Bencao Atlas contains millions of s RNAs from hundreds of plants used in traditional Chinese Medicines(TCMs).

关键词： herbal Atlas

Effect of Peroxiredoxin 1 on the biological function of airway epithelial cells and epithelial-mesenchymal transition

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BIOCELL 2022年第12期46卷 2671-2680页

作者： HUOGEN LIU YUNDI SHI XIN WAN YING LIU HAILIN SHU fengming huang ZHENBIN GONG LING GU Department of Critical Care Medicine Mindong Hospital Affiliated to Fujian Medical UniversityNingde355000China

Peroxiredoxin 1(PRDX1)participates in tumor cell proliferation,apoptosis,migration,invasion,and the epithelial-to-mesenchymal transition(EMT).This study aimed to investigate the effect of PRDX1 on the EMT of airway epithelial cells stimulated with lipopolysaccharide(LPS)and transforming growth factor-beta 1(TGF-β1).PRDX1 overexpression significantly increased the proliferation and migration of human bronchial epithelial(BEAS-2B)cells,reduced cell apoptosis(p<0.01),and induced EMT and collagen deposition by upregulating the expression of the matrix metallopeptidase(MMP)2,MMP9,α-smooth muscle actin(α-SMA),N-cadherin,vimentin and twist proteins and inhibiting E-cadherin expression(p<0.05).PRDX1 overexpression promoted TGF-β1-mediated inhibition of cell proliferation and migration and significantly enhanced the TGF-β1-induced EMT and collagen synthesis(p<0.05).Knockdown of PRDX1 inhibited cell proliferation,migration,EMT,and collagen synthesis(p<0.01),reversed LPS-mediated inhibition of cell proliferation and migration,and significantly suppressed LPS-induced EMT and collagen synthesis(p<0.01).The result indicating that PRDX1 may be involved in LPS/TGF-1-induced EMT and collagen synthesis in human bronchial epithelial cells.

关键词： Peroxiredoxin 1 Airway epithelial cell Epithelial-to-mesenchymal transition Cell migration Collagen synthesis

Large-scale analysis of small RNAs derived from traditional Chinese herbs in human tissues

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Science China(Life Sciences) 2019年第3期62卷 321-332页

作者： fengming huang Jianchao Du Zhu Liang Zhichao Xu Jiantao Xu Yan Zhao Yexuan Lin Song Mei Quan He Jindong Zhu Qiang Liu Yanxu Zhang Yuhao Qin Wei Sun Jingyuan Song Shilin Chen Chengyu Jiang State Key Laboratory of Medical Molecular Biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Department of Biochemistry School of Basic Medicine Peking Union Medical College Tsinghua University Institute of Medicinal Plant Development Chinese Academy of Medical Sciences & Peking Union Medical College Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences

Plant-derived microRNAs have recently been reported to function in human blood and tissues. Controversy was immediately raised due to possible contamination and the lack of large sample sizes. Here, we report thousands of unique small RNAs derived from traditional Chinese medicine(TCM) herbs found in human blood cells and mouse lung tissues using a large-scale *** extracted small RNAs from decoctions of 10 TCM plants(Ban Zhi Lian, Chai Hu, Chuan Xin Lian, Di Ding Zi Jin, huang Qin, Jin Yin Hua, Lian Qiao, Pu Gong Ying, Xia Ku Cao, and Yu Xing Cao) and obtained millions of RNA sequences from each herb. We also obtained RNA-Seq data from the blood cells of humans who consumed herbal decoctions and from the lung tissues of mice administered RNAs from herbal decoctions via oral gavage. We identified thousands of unique small RNA sequences in human blood cells and mouse lung tissues. Some of these identified small RNAs from Chuan Xin Lian and Hong Jing Tian could be mapped to the genomes of the herbs, confirming their TCM plant origin. Small RNAs derived from herbs regulate mammalian gene expression in a sequence-specific manner, and thus are a superior novel class of herbal drug components that hold great potential as oral gene-targeted therapeutics, highlighting the important role of herbgenomics in their development.

关键词： small RNA herbal decoction cross-kingdom regulation miRNA traditional Chinese medicine gene-targeted therapy

Herbal small RNAs in patients with COVID-19 linked to reduced DEG expression

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Science China(Life Sciences) 2023年第8期66卷 1280-1289页

作者： Xiangyu Qiao fengming huang Xiaohu Shi Xingyu Deng Cong Zhang Song Mei Zhiqing Wang Congzhao Zhou Chengyu Jiang Xinghua Tan State Key Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical SciencesDepartment of BiochemistrySchool of Basic Medicine Peking Union Medical CollegeBeijing100005China Peking Union Medical College Hospital Chinese Academy of Medical SciencesBeijing100730China Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences University of Science and Technology of ChinaHefei230027China Guangzhou Eighth People’s Hospital Guangzhou Medical UniversityGuangzhou510440China

In China,more than 80%of patients with coronavirus disease 2019(COVID-19)received traditional Chinese medicine(TCM)as a treatment and their clinical efficacy have been ***,the underlying molecular mechanism remains *** studies have identified herbal small RNAs(s RNAs)as novel functional *** this study,a cohort of 22patients with COVID-19 treated with Toujie Quwen(TQ)granules was *** observed thousands of herbal small RNAs that entered the blood cells of patients after the consumption of TQ *** response to this treatment,the reduced differentially expressed genes(DEGs)were highly correlated with the predicted target genes of the most prevalent herbal s RNAs detected in the ***,the predicted target genes of the top 30 s RNAs from each of the 245 TCMs in the Bencao s RNA Atlas overlapped with 337 upregulated DEGs in patients with mild COVID-19,and 33 TCMs,with more than 50%overlapping genes were identified as effective *** predicted target genes of top 30 s RNAs from Juhong,Gualoupi and Foshou were confirmed *** results not only elucidated a novel molecular mechanism of TCM potential clinical efficacy for COVID-19 patients,but also provided 33 effective COVID-19 TCMs for prescription optimization.

关键词： traditional Chinese medicine Toujie Quwen granules CovID-19 transcriptome

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Orally administered BZL-s RNA-20 oligonucleotide targeting TLR4 effectively ameliorates acute lung injury in mice

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Science China(Life Sciences) 2023年第7期66卷 1589-1599页

作者： Dandan Zhao Yuhao Qin Jiaqi Liu Kegong Tang Shuaiyao Lu Zirui Liu Yexuan Lin Cong Zhang fengming huang Jiahui Chang Chang Li Mingyao Tian Yiming Ma Xiaoyun Li Congzhao Zhou Xiao Li Xiaozhong Peng Ningyi Jin Chengyu Jiang State Key Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical SciencesDepartment of BiochemistryPeking Union Medical CollegeBeijing 100005China Institute of Medical Biology Chinese Academy of Medical SciencesKunming 650031China Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences University of Science and Technology of ChinaHefei 230027China Changchun Veterinary Research Institute Chinese Academy of Agricultural SciencesChangchun 130122China

The global COVID-19 pandemic emerged at the end of December *** respiratory distress syndrome(ARDS)and acute lung injury(ALI)are common lethal outcomes of bacterial lipopolysaccharide(LPS),avian influenza virus,and SARS-Co ***-like receptor 4(TLR4)is a key target in the pathological pathway of ARDS and *** studies have reported that herbal small RNAs(s RNAs)are a functional medical ***-s RNA-20(Accession number:B59471456;Family ID:F2201.Q001979.B11)is a potent inhibitor of Toll-like receptor 4(TLR4)and pro-inflammatory ***,BZLs RNA-20 reduces intracellular levels of cytokines induced by lipoteichoic acid(LTA)and polyinosinic-polycytidylic acid(poly(I:C)).We found that BZL-s RNA-20 rescued the viability of cells infected with avian influenza H5N1,SARS-Co V-2,and several of its variants of concern(VOCs).Acute lung injury induced by LPS and SARS-Co V-2 in mice was significantly ameliorated by the oral medical decoctosome mimic(bencaosome;sphinganine(d22:0)+BZL-s RNA-20).Our findings suggest that BZLs RNA-20 could be a pan-anti-ARDS/ALI drug.

关键词： oligonucleotide drug herbal medicine ARDS/ALI SARS-CoV-2

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氟硅酸钠热分解法生产氟化钠工艺技术

无机盐工业 2013年第12期45卷 31-33页

作者：黄凤鸣昆明阳光安全科技工程有限公司云南昆明650224

介绍了采用氟硅酸钠和碳酸钠在一定温度下进行复分解反应制备氟化钠同时副产白炭黑的工艺技术。以年产3 000 t氟化钠同时副产450 t白炭黑项目为例,以外购的氟硅酸钠为原料,介绍了工艺技术和生产操作要点。项目实际生产运行情况表明,生... 详细信息

介绍了采用氟硅酸钠和碳酸钠在一定温度下进行复分解反应制备氟化钠同时副产白炭黑的工艺技术。以年产3 000 t氟化钠同时副产450 t白炭黑项目为例,以外购的氟硅酸钠为原料,介绍了工艺技术和生产操作要点。项目实际生产运行情况表明,生产运行期间各项经济技术指标均达到了设计要求,氟化钠产品质量达到YS/T 517—2009《氟化钠》各项指标要求,白炭黑产品质量达到HG/T 3061—2009《橡胶配合剂沉淀水合二氧化硅》各项指标要求。如果将该项目与磷肥企业配套建设,可以有效利用磷肥企业的附属产品氟硅酸钠,进而生产高附加值的氟化钠产品,同时副产白炭黑产品,延伸磷肥企业附属产品的产业链。

关键词：氟化钠白炭黑氟硅酸钠磷化工

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真空蒸发技术在氟硅酸镁生产中的应用

无机盐工业 2007年第6期39卷 54-56页

作者：黄凤鸣晋宁昆阳海源工贸有限公司云南晋宁650408

氟硅酸镁生产中蒸发浓缩原先使用的是敞开式蒸发器,不仅热效率低,而且环境污染严重,经过改造采用改性高分子石棉酚醛塑料材质的外加热自然循环真空蒸发器,并对两种蒸发技术进行了比较。详细介绍了负压真空蒸发技术在氟硅酸镁生产中的应... 详细信息

氟硅酸镁生产中蒸发浓缩原先使用的是敞开式蒸发器,不仅热效率低,而且环境污染严重,经过改造采用改性高分子石棉酚醛塑料材质的外加热自然循环真空蒸发器,并对两种蒸发技术进行了比较。详细介绍了负压真空蒸发技术在氟硅酸镁生产中的应用。产品质量优于改造前,成品成本下降200元/t,塑料材质满足耐温、耐腐蚀要求,不仅吨产品的蒸汽耗量节约2/3,而且大大改善了生产环境。

关键词：氟硅酸镁真空蒸发改性高分子石棉酚醛塑料

Transient impact responses of laminated composite cylindrical shells

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Theoretical & Applied Mechanics Letters 2011年第3期1卷 23-26页

作者： Chunchuan Liu,fengming Li,~(a)) and Wenhu huang School of Astronautics,Harbin Institute of Technology,Harbin 150001,China School of Astronautics Harbin Institute of Technology Harbin 150001 China

The generalized ray method(GRM) has been successfully used to study the transient elastic wave transmitting in the beams,planar trusses,space frames and infinite layered *** this letter,the GRM is extended to investigate the early short time transient responses of laminated composite cylindrical shells under impact *** using the Laplace transformation and referring to the boundary conditions,the ray groups transmitting in the finite laminated cylindrical shells under the shock load are obtained and the transient response related to each ray group can be derived via FFT *** the numerical results,it is shown that the early short time transient accelerations of the laminated composite cylindrical shell under impact loads are very *** the short time transient shear strain and displacement are very small.

关键词： laminated composite cylindrical shells impact load early short time transient response generalized ray method

Clinical and biochemical indexes from 2019-nCoV infected patients linked to viral loads and lung injury

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Science China(Life Sciences) 2020年第3期63卷 364-374页

作者： Yingxia Liu Yang Yang Cong Zhang fengming huang Fuxiang Wang Jing Yuan Zhaoqin Wang Jinxiu Li Jianming Li Cheng Feng Zheng Zhang Lifei Wang Ling Peng Li Chen Yuhao Qin Dandan Zhao Shuguang Tan Lu Yin Jun Xu Congzhao Zhou Chengyu Jiang Lei Liu Shenzhen Key Laboratory of Pathogen and Immunity National Clinical Research Center for Infectious DiseaseState Key Discipline of Infectious DiseaseShenzhen Third People’s HospitalSecond Hospital Affiliated to Southern University of Science and TechnologyShenzhen 518112China Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences University of Science and Technology of ChinaHefei 230027China State Key Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical SciencesDepartment of BiochemistryPeking Union Medical CollegeBeijing 100005China Institute of Microbiology Chinese Academy of SciencesBeijing 100101China Emergence Department Peking Union Medical College Hospital Beijing 100731China

The outbreak of the 2019-nCoV infection began in December 2019 in Wuhan,Hubei province,and rapidly spread to many provinces in China as well as other *** we report the epidemiological,clinical,laboratory,and radiological characteristics,as well as potential biomarkers for predicting disease severity in 2019-nCoV-infected patients in Shenzhen,*** 12 cases of the 2019-nCoV-infected patients developed pneumonia and half of them developed acute respiratory distress syndrome (ARDS).The most common laboratory abnormalities were hypoalbuminemia,lymphopenia,decreased percentage of lymphocytes (LYM) and neutrophils (NEU),elevated C-reactive protein (CRP) and lactate dehydrogenase (LDH),and decreased CD8 *** viral load of 2019-nCoV detected from patient respiratory tracts was positively linked to lung disease ***,LYM,LYM (%),LDH,NEU (%),and CRP were highly correlated to the acute lung ***,viral load,lung injury score,and blood biochemistry indexes,albumin (ALB),CRP,LDH,LYM (%),LYM,and NEU (%),may be predictors of disease ***,the Angiotensin Ⅱlevel in the plasma sample from 2019-nCoV infected patients was markedly elevated and linearly associated to viral load and lung *** results suggest a number of potential diagnosis biomarkers and angiotensin receptor blocker (ARB) drugs for potential repurposing treatment of 2019-nCoV infection.

关键词： 2019-nCoV AngiotensinⅡ ARDS