检索结果-南通市图书馆

Rational design of a visible-light photochromic diarylethene:a simple strategy by extending conjugation with electron donating groups

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2019年第4期62卷 451-459页

作者： Shanliang Tang fengling song Meiheng Lu Keli Han Xiaojun Peng State Key Laboratory of Fine Chemicals Dalian University of Technology State Key Laboratory of Molecular Reaction Dynamics Dalian Institute of Chemical Physics Chinese Academy of Sciences

Photochromic diarylethenes have been widely used in many fields. However, their cyclization process must be induced by UV light. In this article, a simple strategy is developed by extending π-conjugation with electron donating groups. The modified dirylethene derivative can photocyclolize under 405-nm light with a good photochromic efficiency. Meanwhile, its absorption and moderate fluorescence can be switched effectively in both directions by visible lights(405 and 520 nm, respectively) in different solutions and in living cells. We believe that this simple method will become a versatile strategy for developing various dirylethylenes with visible-light photochromism.

关键词： diarylethylenes visible-light photochromism photoreaction quantum yield fluorescence photoswitching

Fine-tailoring the linker of near-infrared fluorescence probes for nitroreductase imaging in hypoxic tumor cells

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2017年第10期28卷 1997-2000页

作者： fengling song Ri Liang Jundie Deng Zhiwei Liu Xiaojun Peng State Key Laboratory of Fine Chemicals Dalian University of Technology Dalian 116024 China

Imaging hypoxia using fluorescence probes for nitroreductase（NTR） have attracted much attention in last decade. At least three different linkers have been commonly used to connect the recognition unit and reporting unit in reported probes for NTR. Meanwhile, the linker is known to be a key factor for achieving best sensing performance. In this work, three near-infrared fluorescence probes CyNP-1, CyNP-2 and CyNP-3 were designed and synthesized from an aminocyanine dye CyNP. The three probes have the same recognition unit and same fluorescence reporting unit, but different linkers. CyNP-1 was found to have the best sensing performance for NTR with 40-fold of fluorescence enhancement. It is well investigated how the difference of the linkers brings out the different sensing performance by HPLC, MS and docking calculations. In the end, CyNP-1 was found to have good selectivity for NTR and used to imaging hypoxia in Hela cells.

关键词： Hypoxia Fluorescence probes Nitroreductase Near-infrared Aminocyanine dyes

Self-assembly of amphiphilic peptides to construct activatable nanophotosensitizers for theranostic photodynamic therapy

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2021年第12期32卷 3903-3906页

作者： Shuang Chen Yongzhuo Liu Ri Liang Gaobo Hong Jing An Xiaojun Peng Wen-Heng Zheng fengling song State Key Laboratory of Fine Chemicals Dalian University of TechnologyDalian 116024China Shandong Collaborative Innovation Center of Eco-Chemical Engineering College of Chemical EngineeringQingdao University of Science and TechnologyQingdao 266042China Department of Medical Imaging Cancer Hospital of China Medical UniversityLiaoning Cancer Hospital and InstituteShenyang 110042China Institute of Frontier and Interdisciplinary Science Shandong UniversityQingdao 266237China

A variety of nano-engineered photosensitizers have been developed for photodynamic therapy(PDT)of cancer diseases. However, traditional nano-engineering methods usually cannot avoid drug leakage and premature release, and have disadvantages such as low drug load and inaccurate release.The self-assembly strategy based on amphiphilic peptides has been considered to be more attractive nano-engineering method. Here we developed novel acid-activatable self-assembled nanophotosensitizers based on an amphiphilic peptide derivative. The peptide derivative was synthesized from a fluorescein molecule with thermally activated delayed fluorescence(TADF). The self-assembled nanophotosensitizers can specifically enter the tumor cells and disassemble inside lysosomes companied with “turn-on” fluorescence and photodynamic therapy effect. Such smart nanophotosensitizers will open new opportunities for cancer theranostics.

关键词： Photodynamic therapy Nanophotosensitizer Peptides Self-assembly Acid-activatable

光敏剂DCFP-Cl介导的光动力治疗在胆管癌中应用的实验研究

在线全文

学校读者我要写书评

暂无评论

现代肿瘤医学 2024年第11期32卷 1955-1963页

作者：吴琳舒禹铭郭航成田野宋锋玲郑文恒大连理工大学附属肿瘤医院(辽宁省肿瘤医院)介入科辽宁沈阳110042 中国医科大学辽宁沈阳110122 大连理工大学附属肿瘤医院(辽宁省肿瘤医院)结直肠外科辽宁沈阳110042 山东大学前沿交叉科学研究院山东青岛266237

目的:探究光敏剂DCFP-Cl介导的光动力治疗(photodynamic therapy,PDT)对胆管癌细胞系的生物安全性和有效性。方法:人肝胆管癌细胞(RBE)与DCFP-Cl共培养,观察DCFP-Cl在细胞内的荧光成像潜力,引入Mito-Tracker Green观察DCFP-Cl的细胞器... 详细信息

目的:探究光敏剂DCFP-Cl介导的光动力治疗(photodynamic therapy,PDT)对胆管癌细胞系的生物安全性和有效性。方法:人肝胆管癌细胞(RBE)与DCFP-Cl共培养,观察DCFP-Cl在细胞内的荧光成像潜力,引入Mito-Tracker Green观察DCFP-Cl的细胞器靶向性;使用Calcein AM、DCFH-DA试剂盒检测PDT后细胞活性及细胞内单态氧产生情况;使用CCK-8法定量评估DCFP-Cl对细胞的暗毒性、光动力效率;通过FACS法初步探索DCFP-Cl介导光动力的作用机制;构建荷瘤裸鼠模型,进行动态活体荧光成像,观察DCFP-Cl体内荧光成像能力,通过分组治疗,观察DCFP-Cl对活体肿瘤的抑制作用及生物安全性。结果:在RBE细胞内可见DCFP-Cl的红色荧光与Mito-Tracker Green的绿色荧光重叠,皮尔森相关系数为0.78±0.07,呈强相关;接受PDT组,细胞活力检测实验中未见明显绿色荧光,活性氧实验中显示出明显的绿色荧光;在光照下,随着DCFP-Cl浓度梯度增加RBE细胞存活率逐渐降低,IC50为7.12μmol/L;通过FACS分析,DCFP-Cl介导的PDT诱导细胞死亡的方式主要为细胞凋亡,总体凋亡率随辐照时间延长而增加(t=5.726,P<0.05);荷瘤裸鼠通过尾静脉注射DCFP-Cl,2 h后DCFP-Cl开始在肿瘤区域积聚,以8 h聚集效果最佳;分组治疗中,PDT组肿瘤的大小明显小于其他三组(F=26.82,P<0.001),其HE染色中肿瘤细胞膜不完整,TUNEL染色中可见明显的细胞凋亡,且该组裸鼠主要脏器均未受到损害。结论:DCFP-Cl介导的PDT在胆管癌的体内、外实验中均展现了良好的生物低暗毒性、稳定的荧光成像能力和优异的抗肿瘤活性。

关键词：胆管癌荧光成像光动力治疗光敏剂线粒体

Aquaporin-3 promotes proliferation and inflammation in hepatocellular carcinoma

在线全文

学校读者我要写书评

暂无评论

Genes & Diseases 2024年第4期11卷 45-48页

作者： Yao Wang Yalan Wu Xueying Li Weiwei song fengling Zheng Xiaoying Mo Yi Luo Yun Li song Chen Huanhuan Luo School of Basic Medical Sciences Guangzhou University of Chinese MedicineGuangzhouGuangdong 510006China The School of Health Guangzhou Vocational University of Science andTechnologyGuangzhouGuangdong 510555China Zhongshan Hospital of Traditional Chinese Medicine ZhongshanGuangdong 528401China The Journal Center Guangzhou University of Chinese MedicineGuangzhouGuangdong 510006China Jiangsu Vocational College of Medicine YanchengJiangsu 224005China Science and Technology Innovation Center Guangzhou University of Chinese MedicineGuangzhouGuangdong 510006China State Key Laboratory of Dampness Syndrome of Chinese Medicine GuangzhouGuangdong 510006China

Carcinogenesis is closely associated with inflammation.Studies have shown that lipopolysaccharides(LPS)can contribute to hepatocellular carcinoma(HC)development by inducing IL-6 and TNF-αproduction from the hepatic progenitor cells.1 Aquaporin 3(AQP3),a water channel protein,has recently been found to play a critical role in cancer-associated inflammation.

关键词： inflammation. hepatocellular inflammation

Amphipathic Phenylalanine-Induced Nucleophilic-Hydrophobic Interface Toward Highly Reversible Zn Anode

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano-Micro Letters 2024年第9期16卷 95-109页

作者： Anbin Zhou Huirong Wang fengling Zhang Xin Hu Zhihang song Yi Chen Yongxin Huang Yanhua Cui Yixiu Cui Li Li Feng Wu Renjie Chen Beijing Key Laboratory of Environmental Science and Engineering School of Materials Science and EngineeringBeijing Institute of TechnologyBeijing 100081People’s Republic of China Advanced Technology Research Institute Beijing Institute of TechnologyJinan 250300People’s Republic of China Collaborative Innovation Center of Electric Vehicles in Beijing Beijing 100081People’s Republic of China Institute of Electronic Engineering China Academy of Engineering PhysicsMianyang 621900People’s Republic of China

Aqueous Zn^(2+)-ion batteries(AZIBs),recognized for their high security,reliability,and cost efficiency,have garnered considerable attention.However,the prevalent issues of dendrite growth and parasitic reactions at the Zn electrode interface significantly impede their practical application.In this study,we introduced a ubiquitous biomolecule of phenylalanine(Phe)into the electrolyte as a multifunctional additive to improve the reversibility of the Zn anode.Leveraging its exceptional nucleophilic characteristics,Phe molecules tend to coordinate with Zn^(2+)ions for optimizing the solvation environment.Simultaneously,the distinctive lipophilicity of aromatic amino acids empowers Phe with a higher adsorption energy,enabling the construction of a multifunctional protective interphase.The hydrophobic benzene ring ligands act as cleaners for repelling H_(2)O molecules,while the hydrophilic hydroxyl and carboxyl groups attract Zn^(2+)ions for homogenizing Zn^(2+)flux.Moreover,the preferential reduction of Phe molecules prior to H_(2)O facilitates the in situ formation of an organic-inorganic hybrid solid electrolyte interphase,enhancing the interfacial stability of the Zn anode.Consequently,Zn||Zn cells display improved reversibility,achieving an extended cycle life of 5250 h.Additionally,Zn||LMO full cells exhibit enhanced cyclability of retaining 77.3%capacity after 300 cycles,demonstrating substantial potential in advancing the commercialization of AZIBs.

关键词： Zn anode Phenylalanine Adsorption energy Solvation sheath

FRET Spectral Unmixing:A Ratiometric Fluorescent Nanoprobe for Hypochlorite

在线全文

学校读者我要写书评

暂无评论

FRET Spectral Unmixing:A Ratiometric Fluorescent Nanoprobe f...

A turn-on fluorescent probe for Au^(3+) based on rodamine derivative and its bioimaging application

第八届全国化学生物学学术会议

作者： Gengwen Chen Xiaojun Peng fengling song State Key Laboratory of Fine Chemicals Dalian University of Technology

＜正＞In the field of biological research and technology,Forster resonance energy transfer （FRET） has been a popular tool,1 whether in vitro or in vivo.Ratio metric fluorescent probes based on FRET allow the measurement of emission intensities at two different wavelengths,which should provide a built-in correction for environmental effect and can also increase the dynamic range of fluorescence measurement.However,an undesirable overlap between these two emissions often complicates the determination of FRET efficiencies and cause misleading results.Although linear unmixing algorithms are available for correcting experiments’ results caused by signals bleed-through,the crosstalk of the FRET pairs’ emissions still makes FRET ratio metric approach not accurate.Here we describe a new construction method for FRET probe by loading two fluorophores on a hydrid nanoparticle to realize a true spectral unmixing and exhibit its chemo-sensing properties for hypochlorite. We proposed a new spectral unmixing approach by developing a new ratiometric fluorescence nanoprobe for hypochlorite based on the chemo sensing response of an aminocyanine dye towards ROS.4 The nanoprobe exhibited a ratiometric fluorescence response to hypochlorite by determination the value of 1575/1750.As we know,such an unprecedented big separation of FRET pairs’ emissions has not been reported before.

关键词： FRET Nanoprobe Hypochrite Spectral Unmixing Cyanine dye

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Science China Chemistry 2014年第7期57卷 1043-1047页

作者： song fengling NING HouFu SHE HaiYing WANG JingYun PENG XiaoJun State Key Laboratory of Fine Chemicals Dalian University of Technology School of Life Science & Biotechnology Dalian University of Technology

A new fluorescent probe RY was synthesized for the detection of Au3+ions based on a rhodamine B derivative.The fluorescent probe showed good selectivity and sensitivity to Au3+ions.Obvious color and fluorescence changes could be observed with the naked eye while the fluorescent probe reacted with the Au3+ions.The detection limit of the probe was determined to be 36 ppb by the fluorescence titration.The excellent linear relationship suggests that the probe is potentially useful for quantitative detection of Au3+in vitro.We also demonstrated its bioimaging application in both living cells and mice.This was the first time that a fluorescent probe was successfully applied to imaging Au3+in living animals.

关键词：荧光探针生物成像罗丹明B 衍生物应用荧光滴定法肉眼观察荧光变化

Ratiometric Fluorescent Probe Based on Novel Red-emission BODIPY for Determination of Bovine Serum Albumin

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2014年第5期30卷 738-742页

作者： song fengling XUE Yingying WANG Xu WANG Jingyun XIONG Xiaoqing PENG Xiaojun State Key Laboratory of Fine Chemicals Dalian University of Technology Dalian 116024 P. R. China

A novel red-emission boron-dipyrromethene（BODIPY） dye with a pyrrole ring was synthesized simply via one-pot reaction. The spectral properties of it were investigated under the conditions of different solvents. The results show that the as-prepared BODIPY dye is extremely sensitive to solvent polarity, and the fluorescent emission enhances with the decrease of solvent polarity. In aqueous buffer, the addition of bovine serum albumin leads to a ratiometric change in absorption spectra with an association constant of 1.16×10^6 L/mol. Meanwhile, the fluorescence emission increases greatly at 622 nm but changes slightly at 575 nm. The response time is very short（less than 3 min）, and the changes of color can be noticed by naked eyes. Bovine serum albumin can be detected by this ratiometric fluorescence probe, but other proteins or enzymes cannot be detected by this method, which indicates that this novel dye has high selectivity towards bovine serum albumin. The reason is that bovine serum albumin has suitable hydro- phobic cavities for binding with the dye. In addition, the dye molecule can penetrate cell membrane easily and make a fast fluorescent stain, which makes it a potential probe for living-cell fluorescence imaging.

关键词： Fluorescent dye Boron-dipyrromethene（BODIPY） Bovine serum albumin Ratiometric High selectivity