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Seed,cutting and air-layering reproductive inefficiency of noxious woody vine Merremia biosiana and its implications for management strategy

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Frontiers in Biology 2009年第3期4卷 342-349页

作者： Mingguang li Haiyang liU fenglan li Xiuyuan CHENG Bin GUO Zhiwei FAN State Key Laboratory of Biocontrol Sun Yat-sen UniversityGuangzhou 510275China Environment and Plant Protection Institute Chinese Academy of Tropical Agricultural SciencesDanzhou 571737China

Merremia boisiana(Gagnep)van *** a noxious fast growing woody vine and is able to grow overtop other plants,causing the death of plants underneath and forming monospecies *** formulate management responses,we assessed its seed and vegetative reproduction efficacy through indoor and field *** number of flowers counted from bagged infructescences in Guangzhou ranged from 25 to 172,with an average of *** the seeds of bagged infructescences had shown that there were only 1.58 hard testa seeds in each *** vitality tests using red ink indicated that only 68.6%of hard testa and filled seeds were *** emergence rate of scarified hard testa seeds in the sand bed was 31.96%.Under imitated natural conditions,8%of hard testa seeds could germinate,and 9%still retained their germination potential in one ***,seedlings should be monitored and removed in a timely fashion after any attempt of *** soil or transplanting plant from infested patches should be strictly prohibited at least for several *** considered,an infructescence contributed 0.3 *** investigation in the field found no seedlings either inside or at the perimeter of the patch,suggesting scant expansion by means of seed ***,the elimination effort could be focused on a relatively restricted scale of *** 630 cuttings of young shoots,old shoots and old lying shoots with or without growth regulators,there were only four(or 0.63%)established ***-layering shoots all died in two *** cuttings and airlayering reproduction indicated that regeneration from fragments of removed stems or accidentally dropped ones was quite unlikely,and thus mechanical removal was safe.

关键词： Merremia boisiana seed vitality germination rate vegetative reproduction management

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Occurrence and light response of residual plastid genes in a Euglena gracilis bleached mutant strain OflB2

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Journal of Oceanology and limnology 2020年第6期38卷 1858-1866页

作者： QIN Huan GUO Qingqing liU Chenchen li fenglan ZHANG Hua CHU Zihan WANG Jiangxin LEI Anping Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science Shenzhen Engineering Laboratory for Marine Algal BiotechnologyGuangdong Provincial Key Laboratory for Plant EpigeneticsCollege of Life Sciences and OceanographyShenzhen UniversityShenzhen 518000China Basis International School SZ Shenzhen 518000China

Euglena gracilis is a unicellular green eukaryotic microalga that features characteristics of both plants and *** photosynthetic function of its chloroplast is easily lost under stress resulting in bleached mutants,while the physiological role of their residual plastid DNAs remains *** this study,we obtained five bleached mutants by ofloxacin(Ofl)treatment,identified 12 residual plastid genes in five bleached mutants,and determined the mRNA levels in the wild type ***(WT)and one bleached mutant(OflB2)under dark and light stimulation conditions by quantitative reverse transcribed PCR(qRTPCR).Results show that the expression of all selected plastid genes in both WT and OflB2 mutant did not change significantly in darkness,while their responses to light stimulation were *** the light stimulation conditions,half of the genes did not change significantly,while most of the other genes were down-regulated in OflB2 mutant and up-regulated in ***,the bleached mutant retains part of the plastid genome and the plastid relic is responsive to *** research will help to understand the functions of residual plastid DNA and evolution of chloroplasts.

关键词： Euglena gracilis bleached mutant residual plastid genes light response

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Osmotic adjustment and up-regulation expression of stress-responsive genes in tomato induced by soil salinity resulted from nitrate fertilization

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International Journal of Agricultural and Biological Engineering 2018年第3期11卷 126-136页

作者： Tingting Chang Yujie Zhang Huilian Xu Xiaohou Shao Qicong Xu fenglan li lina Yu Zhanyu Zhang Key Laboratory of Efficient Irrigation-Drainage and Agricultural Soil-Water Environment in Southern China of Ministry of Education Hohai UniversityNanjing 210098China College of Agricultural Science and Engineering Hohai UniversityNanjing210098China International Nature Farming Research Center Matsumoto390-1401 Japan Northeast Agricultural University Harbin 150000China Rizhao Institute of Agricultural Sciences Rizhao276800China

Concerns about the soil salinity caused by excessive fertilization have prompted scientists to clarify the detailed mechanisms and find techniques to alleviate the damage caused by this kind of soil *** of this study were to elucidate the effect of soil salinity caused by nitrate fertilization and the differences in salinity effect between nitrate salts and NaCl salt with analyses at various levels of crop physiology and molecular biology.A microbial inoculation was also tried to verify whether it could alleviate the salinity-induced loss and *** three experiments(Exp I,II and III),nitrate salts(NS)of Ca(NO_(3))_(2) and KNO_(3) were applied to potted tomato plants to simulate the soil salinity caused by fertilization and a microbial inoculant(MI)was *** was measured using *** adjustment was analyzed using the mathematically modeled pressure-volume curve;O_(2)-concentration and activity of SOD and nitrate reductase were *** of nitrate reductase gene and the stress-responsive gene DREB2 was analyzed using the real-time PCR *** Exp I and II,where the applied NS amount was moderate,NS application at low concentration induced increases in O_(2)-and MDA concentrations and plants acclimated to the soil salinity as the treatment prolonged for *** acclimation was contributed by osmotic adjustment,activation of SOD and re-compartmentation of cell water between symplasm and *** adjustments might be ultimately attributed to up-regulation of stress-responsive genes such as DREB2 as well as the nitrate reductase(NR)***,in Exp III,applications of NaCl and NS at high concentration could not show positive effects as NS *** of MI synergistically increased the xerophytophysiological regulation caused by NS and alleviated the salinity damage in addition to its own positive effects on the tomato *** from NaCl,nitrate salts at low application rate increased the total b

关键词： nitrate fertilizer osmotic adjustment salinity stress soil salinization tomato(Solanum lycopersicum) xerophytophysiology microorganism bioremediation

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Study on the rapid propagation of Phalaenopsis hybrid

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Journal of Northeast Agricultural University(English Edition) 2007年第1期14卷 14-16页

作者： WEI Qi HU Guofu HUANG fenglan li fenglan HU Baozhong College of life Science Northeast Agricultural University Harbin 150030 China

This paper studied the rapid propagation technology of Phalaenopsis hybrid by using the peduncle buds as the explants, and screened out a kind of culture medium, which was simple, rapid, available and high rate of pro... 详细信息

关键词： Phalaenopsis hybrid peduncle bud PLB rapid propagation

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RAPD analysis on the four kinds of color-flowered Salvia splendens Ker-Gawl

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Journal of Northeast Agricultural University(English Edition) 2007年第2期14卷 97-102页

作者： HU Guofu YUAN Qiang li fenglan WEI Qi HU Baozhong College of life Sciences Northeast Agricultural University Harbin 1500300 China

In this study, we used RAPD to analyze four kinds of color-flowered Salvia splendens Ker-Gawl, and the optimal RAPD reaction conditions were the optimal reaction mixture （25 μL total volume） that contained 2.0 μL 10×buffer, 0.45 mmol·L^-1 dNTPs, 2.0 mmol· L^-1 Mg^2＋, 2 U Taq DNA polymerase, 0.30 umol·L^-2 primer and 40 ng genomic DNA. Total 84 bands were amplified from 12 primers used, and the differential bands had 28 bands, which was 33% of total bands. In cluster group analysis, the four kinds of color-flowered were divided into two styles. One style is that the red color and red-white color were grouped together, then they grouped with purple color into one cluster, and the white color was another style.

关键词： Salvia splendens Ker-Gawl color RAPD analysis cluster group analysis

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Diversity-Oriented Enantioselective Construction of Atropisomeric Heterobiaryls and N-Aryl Indoles via Vinylidene Ortho-Quinone Methides

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CCS Chemistry 2022年第8期4卷 2686-2697页

作者： Da Xu Shengli Huang Fangli Hu Lei Peng Shiqi Jia Hui Mao Xiangnan Gong fenglan li Wenling Qin Hailong Yan Chongqing Key Laboratory of Natural Product Synthesis and Drug Research School of Pharmaceutical SciencesChongqing UniversityChongqing 401331 College of Pharmacy Jinhua PolytechnicJinhuaZhejiang Province 321007 Analytical and Testing Center of Chongqing University Chongqing UniversityChongqing 401331 Anqiu Women and Children’s Hospital AnqiuShandong Province 262102

An atroposelectively diversity-oriented synthetic strategy was developed for the divergent synthesis of axially chiral heterocycles through organocatalytic asymmetric intramolecular annulation of alkyne via vinylidene ortho-quinone methides(VQMs).The methodology reported herein was characterized by rapid reactions(most completed in seconds),high stereocontrol(up to 98%ee),and broad substrate scope(60 examples of different skeletal types,stereogenicelements,andring sizes).

关键词： asymmetric catalysis organocatalysis atroposelectivity axial chirality heteroannulation

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聚丙烯纤维和机制砂中石粉对水泥砂浆抗裂性能的影响

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应用基础与工程科学学报 2012年第5期20卷 895-901页

作者：李凤兰梁娜李长永华北水利水电学院生态建筑材料河南省高校重点实验室培育基地河南郑州450011

通过试验研究了聚丙烯纤维掺量和长度、机制砂中石粉等量替代水泥比对水泥砂浆抗裂性能的影响,考虑了长度8mm的聚丙烯纤维在0.4—1.2kg/m3范围内的6个掺量、长度19mm的聚丙烯纤维在0.8—1.2kg/m3范围内的3个掺量以及在2.5%—15%范围内的... 详细信息

通过试验研究了聚丙烯纤维掺量和长度、机制砂中石粉等量替代水泥比对水泥砂浆抗裂性能的影响,考虑了长度8mm的聚丙烯纤维在0.4—1.2kg/m3范围内的6个掺量、长度19mm的聚丙烯纤维在0.8—1.2kg/m3范围内的3个掺量以及在2.5%—15%范围内的5个石粉等量替代水泥比,采用现行标准试验方法测定了15种新拌水泥砂浆的稠度和分层度以及30个水泥砂浆试件的裂缝宽度和长度,采用开裂指数和抗裂性能比评价水泥砂浆的抗裂性能.结果表明:新拌砂浆均具有良好的工作性能,稠度和分层度均在合理范围内;适当掺量的聚丙烯纤维或采用适量的石粉等量替代水泥,均可有效改善水泥砂浆的抗裂性能,短纤维比长纤维具有更好的改善效果;在一定范围内,随着聚丙烯纤维掺量或石粉等量替代水泥比的增加,水泥砂浆的抗裂性能比增大而开裂指数降低;当聚丙烯纤维掺量为1.0kg/m3或石粉等量替代水泥比为10%时,水泥砂浆的抗裂性能最佳.

关键词：水泥砂浆抗裂性能工作性能聚丙烯纤维石粉

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Construction of Clonal Growth Models in Trifolium repens

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Journal of Northeast Agricultural University(English Edition) 2009年第4期16卷 14-18页

作者： HUBaozhong li fenglan HU Xiaomei HU Guofu YUAN Qiang College of life Sciences Northeast Agricultural University Harbin 150030 China

The suitability of models for describing the clonal growth of Trifolium repens population was discussed. The results showed that deterministic models were inadequate for describing its clonal growth, but the diffusion models and the randomwalk models suited for the clonal growth characteristics of the population. And it was found that random-walk models were better than diffusion models for describing a population in an environment with rich natural resources, and the latter was better in a poor environment.

关键词： Trifolium repens clonal growth model diffusion model random-walk model

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Numerical Solution of Integro-Differential Equations with Local Polynomial Regression

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Open Journal of Statistics 2012年第3期2卷 352-355页

作者： liyun Su Tianshun Yan Yanyong Zhao fenglan li Ruihua liu library Chongqing University of Technology Chongqing China School of Mathematics and Statistics Chongqing University of Technology Chongqing China

In this paper, we try to find numerical solution of y'(x)= p(x)y(x)+g(x)+λ∫ba K(x, t)y(t)dt, y(a)=α. a≤x≤b, a≤t≤b or y'(x)= p(x)y(x)+g(x)+λ∫xa K(x, t)y(t)dt, y(a)=α. a≤x≤b, a≤t≤b by using Local polynomial regression (LPR) method. The numerical solution shows that this method is powerful in solving integro-differential equations. The method will be tested on three model problems in order to demonstrate its usefulness and accuracy.

关键词： Integro-Differential Equations Local Polynomial Regression Kernel Functions

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世界远程教育发展中特别值得我国借鉴的几个关键问题

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中国电化教育 2005年第8期 36-40页

作者：李凤兰中央电化教育馆培训中心北京100031

世界远程教育飞速发展所产生的丰富的理论和实践成果,为我国远程教育发展提供了宝贵的经验。本文着重从远程教育的定位、质量保证等对我国远程教育发展至关重要的若干方面进行了综述和分析。

关键词：远程教育管理与发展关键问题

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