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The PI3K/Akt/GSK-3β/ROS/eIF2B pathway promotes breast cancer growth and metastasis via suppression of NK cell cytotoxicity and tumor cell susceptibility

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Cancer Biology & Medicine 2019年第1期16卷 38-54页

作者： fengjiao jin Zhaozhen Wu Xiao Hu Jiahui Zhang Zihe Gao Xiao Han Junfang Qin Chen Li Yue Wang School of Medicine Nankai University Institute of Biomedical Engineering Chinese Academy of Medical Sciences & Peking Union Medical College State Key Laboratory of Medicinal Chemical Biology Nankai University

Objective: To examine the effect of pSer9-GSK-3β on breast cancer and to determine whether the underlying metabolic and immunological mechanism is associated with ROS/eIF2B and natural killer(NK) ***: We employed TWS119 to inactivate GSK-3β by phosphorylating Ser9 and explored its effect on breast cancer and NK cells. The expression of GSK-3β, natural killer group 2 member D(NKG2D) ligands, eIF2B was quantified by PCR and Western blot. We measured intracellular reactive oxygen species(ROS) and mitochondrial ROS using DCFH-DA and MitoSOX^(TM) probe,respectively, and conducted quantitative analysis of cellular respiration on 4T1 cells with mitochondrial respiratory chain complex Ⅰ/Ⅲ ***: Our investigation revealed that TWS119 downregulated NKG2D ligands(H60 a and Rae1), suppressed the cytotoxicity of NK cells, and promoted the migration of 4T1 murine breast cancer cells. Nevertheless, LY290042, which attenuates p-GSK-3β formation by inhibiting the PI3K/Akt pathway, reversed these effects. We also found that higher expression of p Ser9-GSK-3β induced higher levels of ROS, and observed that abnormality of mitochondrial respiratory chain complex Ⅰ/Ⅲ function induced the dysfunction of GSK-3β-induced electron transport chain, naturally disturbing the ROS level. In addition, the expression of NOX3 and NOX4 was significantly up-regulated, which affected the generation of ROS and associated with the metastasis of breast cancer. Furthermore, we found that the expression of pSer535-eIF2B promoted the expression of NKG2D ligands(Mult-1 and Rae1) following by expression of pSer9-GSK-3β and generation of ***: The PI3K/Akt/GSK-3β/ROS/eIF2B pathway could regulate NK cell activity and sensitivity of tumor cells to NK cells,which resulted in breast cancer growth and lung metastasis. Thus, GSK-3β is a promising target of anti-tumor therapy.

关键词： GSK-3β NK cells NKG2D/NKG2DLs ROS eIF2B breast cancer

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An integrated portable bio-monitoring system based on tough hydrogels for comprehensive detection of physiological activities

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Nano Research 2024年第1期17卷 321-332页

作者： Congcong Yang Chenchen Ji fengjiao Guo Chunjiang jin Hongyu Mi Zhongchang Wang State Key Laboratory of Chemistry and Utilization of Carbon Based Energy Resources School of Chemical Engineering and TechnologyXinjiang UniversityUrumqi 830017China International Iberian Nanotechnology Laboratory(INL) Avenide Mestre Jose VeigaBraga 4715-330Portugal

Advanced soft ion-conducting hydrogels have been developed rapidly in the integrated portable health monitoring equipment due to their higher sensitivity,sensory traits,tunable conductivity,and stretchability for physiological activities and personal healthcare ***,traditional hydrogel conductors are normally susceptible to large deformation and strong mechanical stress,which leads to inferior electro-mechanical stability for real application ***,a strong ionically conductive hydrogel(poly(vinyl alcohol)-boric acid-glycerol/sodium alginate-calcium chloride/electrolyte ions(PBG/SC/EI))was designed by engineering the covalently and ionically crosslinked networks followed by the salting-out effect to further enhance the mechanical strength and ionic conductivity of the *** to the collective effects of the energy-dissipation mechanism and salting-out effect,the designed PBG/SC/EI with excellent structural integrity and robustness exhibits exceptional mechanical properties(elongation at break for 559.1%and tensile strength of 869.4 kPa)and high ionic conductivity(1.618 S·m^(-1)).As such,the PBG/SC/EI strain sensor features high sensitivity(gauge factor=2.29),which can effectively monitor various kinds of human motions(joint motions,facial micro-expression,faint respiration,and voice recognition).Meanwhile,the hydrogel-based Zn||MnO_(2)battery delivers a high capacity of 267.2 mAh·g^(-1)and a maximal energy density of 356.8 Wh·kg^(-1)associated with good cycle performance of 71.8%capacity retention after 8000 ***,an integrated bio-monitoring system with the sensor and Zn||MnO_(2)battery can accurately identify diverse physiological activities in a real-time and non-invasive *** work presents a feasible strategy for designing high-performance conductive hydrogels for highly-reliable integrated bio-monitoring systems with excellent practicability.

关键词： Ionically conductive hydrogel strain sensor Zn-based battery integrated bio-monitoring system human motion monitoring

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Polyanionic hydrogel electrolyte enables reversible and durable Zn anode for efficient Zn-based energy storage

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Journal of Energy Chemistry 2023年第11期86卷 373-381,I0008页

作者： Chunjiang jin Congcong Yang Hongyu Mi Chenchen Ji fengjiao Guo Chengzhe Liu Ziqiang Liu Nianjun Yang State Key Laboratory of Chemistry and Utilization of Carbon Based Energy Resources School of Chemical Engineering and TechnologyXinjiang UniversityUrumqi 830017XinjiangChina Department of Chemistry Hasselt UniversityDiepenbeek 3590Belgium

Aqueous Zn-ion energy storage systems,which are expected to be integrated into intelligent electronics as a secure power supply,suffer poor reversibility of Zn anodes,predominantly associated with dendritic growth and side *** study introduces a polyanionic strategy to address these formidable issues by developing a hydrogel electrolyte(PACXHE)with carboxyl ***,the carboxyl groups within the hydrogel structure establish favorable channels to promote the transport of Zn^(2+)*** also expedite the desolvation of hydrated Zn^(2+)ions,leading to enhanced deposition ***,these functional groups confine interfacial planar diffusion and promote preferential deposition along the(002)plane of Zn,enabling a smooth surface texture of the Zn *** multifaceted regulation successfully achieves the suppression of Zn dendrites and side reactions,thereby enhancing the electrochemical reversibility and service life during plating/stripping ***,such an electrolyte demonstrates a high average Coulombic efficiency of 97.7%for 500 cycles in the Zn‖Cu cell and exceptional cyclability with a duration of 480 h at 1 mA cm^(-2)/1 mA h cm^(-2)in the Zn‖Zn *** that,the Zn-ion hybrid micro-capacitor employing PACXHE exhibits satisfactory cycling stability,energy density,and practicality for energy storage in flexible,intelligent *** present polyanionic-based hydrogel strategy and the development of PACXHE represent significant advancements in the design of hydrogel electrolytes,paving the way for a more sustainable and efficient future in the energy storage field.

关键词： Polyanionic hydrogel electrolyte Zinc anode issues Dendrite suppression Electrochemical performance Zinc-ionhybrid micro-capacitor

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Assessment of drought and its impact on winter wheat yield in the Chinese Loess Plateau

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Journal of Arid Land 2022年第7期14卷 771-786页

作者： WANG fengjiao FU Bojie LIANG Wei jin Zhao ZHANG Liwei YAN Jianwu FU Shuyi GOU Fen School of Geography and Tourism Shaanxi Normal UniversityXi'an 710119China State Key Laboratory of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China National Demonstration Center for Experimental Geography Education Shaanxi Normal UniversityXi'an 710119China State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau Northwest A&F UniversityYangling 712100China School of Geography and Planning Sun Yat-Sen UniversityGuangzhou 510275China

Drought has pronounced and immediate impacts on agricultural production,especially in semi-arid and arid rainfed agricultural *** of drought and its impact on crop yield is essential to agricultural water resource management and food *** investigated drought and its impact on winter wheat(Triticum aestivum L.)yield in the Chinese Loess Plateau from 2001 to ***,we performed a varimax rotated principal component analysis on drought severity index(DSI)separately for four winter wheat growth periods:pre-sowing growth period(PG),early growth period(EG),middle growth period(MG),and late growth period(LG),resulting in three major subregional DSI dynamics for each growth *** county-level projections of these major dynamics were then used to evaluate the growth period-specific impacts of DSI on winter wheat yields by using multiple linear regression *** results showed that the growth period-specific subregions had different major DSI *** PG,the northwestern area exhibited a rapid wetting trend,while small areas in the south showed a slight drying *** remaining subregions fluctuated between dryness and *** EG,the northeastern and western areas exhibited a mild wetting *** remaining subregions did not display clear wetting or drying *** MG,the eastern and southwestern areas showed slight drying and wetting trends,*** subregions scattered in the north and south had a significant wetting *** LG,large areas in the east and west exhibited wetting trends,whereas small parts in south-central area had a slight drying *** counties in the north showed significant and slight wetting trends during PG,EG,and LG,whereas a few southwestern counties exhibited significant drying trends during PG and *** analysis identified close and positive relationships between yields and DSI during LG,and revealed that almost all of the counties were vulnerable to ***

关键词： drought severity index winter wheat crop yield principal component analysis Loess Plateau

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Impacts of Park Landscape Structure on Thermal Environment Using QuickBird and Landsat Images

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Chinese Geographical Science 2017年第5期27卷 818-826页

作者： XU Xinliang CAI Hongyan QIAO Zhi WANG Liang jin Cui GE Yaning WANG Luyao XU fengjiao State Key Laboratory of Resources and Environmental Information Systems Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101China School of Environmental Science and Engineering Tianjin UniversityTianjin 300072China University of Chinese Academy of Sciences Beijing 100049China College of Urban and Environ-mental Science Liaoning Normal UniversityDalian 116029China School of Soil and Water Conservation Beijing Forestry UniversityBeijing 100083China College of Urban and Environmental Sciences Central China Normal UniversityWuhan 430079China Geography Department College of Sciences Yanbian UniversityYanji 133002China

Urban parks composed mostly of vegetation and water bodies can effectively mitigate the urban heat island effect. Many studies have investigated the cooling effects of urban parks; however, little attention has been given to park landscape structure. Based on landscape metrics, this study has explored the influences of the park landscape structure on its inner thermal environment, taking heavily urbanized Beijing Municipality in China as the study area. Three indices, including the percentage of landscape （PLAND）, landscape shape index （LSI） and aggregation index （AI）, were used to measure the composition and configuration characteristics of the landscape components inside the parks. The indices were calculated for five landscape types being interpreted from Quickbird images. Urban thermal conditions were measured using the land surface temperature （LST） derived from Landsat TM images. The results showed that the park LST had a negative relationship with the park size, but no significant relationship was found with park shape. For the park＇s interior landscape, however, the configuration and composition characteristics of the landscape components inside the park explained 70% of the park LST variance. The area percentage of water bodies and the aggregation index of woodland were identified as the key influencing characteristics. In addition, when the composition and configuration characteristics of the park landscape components were separately considered, the configuration characteristics （LSI and A1） explained approximately 54% of the variance in park LST, which was comparable with that explained by the composition characteristics （PLAND）. Thus, this study suggested that an effective and practical way for urban cooling park design is the optimization of spatial configuration of landscape components inside the park.

关键词： urban park urban heat island park cooling effect landscape metrics Quickbird images Beijing

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Shikimic acid accelerates phase change and flowering in Chinese jujube

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Horticultural Plant Journal 2024年第2期10卷 413-424页

作者： Xianwei Meng Zhiguo Liu Li Dai Weiqiang Zhao Jiurui Wang Lili Wang Yuanpei Cui Ying Li Yinshan Cui Yao Zhang Luyao Wang fengjiao Yu jin Zhao Mengjun Liu Research Center of Chinese Jujube Hebei Agricultural UniversityBaodingHebei 071000China College of Horticulture Hebei Agricultural UniversityBaodingHebei 071000China College of Life Science Hebei Agricultural UniversityBaodingHebei 071000China College of Forestry Hebei Agricultural UniversityBaodingHebei 071000China eCollege of Economics and ManagementHebei Agricultural UniversityBaodingHebei 071000China College of Economics and Management Hebei Agricultural University

The juvenile-to-adult phase change with first flowering as the indicator plays a crucial role in the lifecycle of fruit trees. However, the molecular mechanisms underlying phase change in fruit trees remain largely unknown. Shikimic acid (ShA) pathway is a main metabolic pathway closely related to the synthesis of hormones and many important secondary metabolites participating in plant phase change. So,whether ShA regulates phase change in plants is worth clarifying. Here, the distinct morphological characteristics and the underlying mechanisms of phase change in jujube (Ziziphus jujuba Mill.), an important fruit tree native to China with nutritious fruit and outstanding tolerance abiotic stresses, were clarified. A combined transcriptome and metabolome analysis found that ShA is positively involved in jujube(Yuhong’×Xing 16’) phase change. The genes in the upstream of ShA synthesis pathway (ZjDAHPS, ZjDHQS and ZjSDH), the contents of ShA and the downstream secondary metabolites like phenols were significantly upregulated in the phase change period. Further, the treatment of spraying exogenous ShA verified that ShA at a very low concentration (60 mg·L^(-1)) can substantially speed up the phase change and flowering of jujube and other tested plants including Arabidopsis, tomato and wheat. The exogenous ShA (60 mg·L^(-1)) treatment in jujube seedlings could increase the accumulation of endogenous ShA, enhance leaf photosynthesis and the synthesis of phenols especially flavonoids and phenolic acids, and promote the expression of genes (ZjCOs, ZjNFYs and ZjPHYs) involved in flowering pathway. Basing on above results, we put forward a propose for the underlying mechanism of ShA regulating phase change, and a hypothesis that ShA could be considered a phytohormone-like substance because it is endogenous, ubiquitous, movable and highly efficient at very low concentrations. This study highlights the critical role of ShA in plant phase change and its phytohormone-like propert

关键词： Ziziphus jujuba Mill. Phase change Flowering Shikimic acid Transcriptome Metabolome

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Generation of B Cell-Deficient Pigs by Highly Efficient CRISPR/Cas9-Mediated Gene Targeting

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Journal of Genetics and Genomics 2015年第8期42卷 437-444页

作者： fengjiao Chen Ying Wang Yilin Yuan Wei Zhang Zijian Ren Yong jin Xiaorui Liu Qiang Xiong Qin Chen Manling Zhang Xiaokang Li Lihua Zhao Ze Li Zhaoqiang Wu Yanfei Zhang Feifei Hu Juan Huang Rongfeng Li Yifan Dai State Key Laboratory of Reproductive Medicine and Jiangsu Key Laboratory of Xenotransplantation Nanjing Medical University

Generating B cell-deficient mutant is the first step to produce human antibody repertoires in large animal models. In this study, we applied the clustered regularly interspaced short palindromic repeat （CRISPR）/CRISPR-associated （Cas） system to target the JH region of the pig IgM heavy chain gene which is crucial for B cell development and differentiation. Transfection of IgM-targeting Cas9 plasmid in primary porcine fetal fibroblasts （PFFs） enabled inducing gene knock out （KO） in up to 53.3% of colonies analyzed, a quarter of which harbored biallelic modification, which was much higher than that of the traditional homologous recombination （HR）. With the aid of somatic cell nuclear transfer （SCNT） technology, three piglets with the biallelic lgM heavy chain gene mutation were produced. The piglets showed no antibody-producing B cells which indicated that the biallelic mutation of the lgM heavy chain gene effectively knocked out the function of the IgM and resulted in a B cell-deficient phenotype. Our study suggests that the CRISPR/Cas9 system combined with SCNT technology is an efficient genome-editing approach in pigs.

关键词： CRISPR/Cas9 system lgM heavy chain Pig genome editing B cell-deficiency Somatic cell nuclear transfer

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耳蜗神经网络中语音信号传输的刺激条件信息研究

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复杂系统与复杂性科学 2015年第4期12卷 104-108页

作者：王风娇任昱昊赵进段法兵青岛大学复杂性科学研究所山东青岛266071

在耳蜗神经网络对语音信号的刺激响应过程中,针对如何区分编码最有效率的语音信号分量问题,提出了刺激条件信息分布计算方法,研究了给定刺激条件下平均不确定性度的减小。实验结果表明:积分发放神经网络膜电位发放的刺激条件信息不仅能... 详细信息

在耳蜗神经网络对语音信号的刺激响应过程中,针对如何区分编码最有效率的语音信号分量问题,提出了刺激条件信息分布计算方法,研究了给定刺激条件下平均不确定性度的减小。实验结果表明:积分发放神经网络膜电位发放的刺激条件信息不仅能够从统计意义上给出平均互信息的大小,而且清晰地表明信号中各分量的编码效率,确定输入信号中对于互信息量起主要作用的事件分量范围以及内部噪声的可利用性,证实噪声强度与最大刺激条件信息量之间的非单调关系,这些研究结果为进一步探索人工耳蜗动作电位发放的解码方案提供了理论依据。

关键词：耳蜗神经网络语音信号积分发放神经元刺激条件信息

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服务生态系统价值共创单元的共生演化模型

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计算机集成制造系统 2022年第5期28卷 1549-1561页

作者：孙凤娇何霆晋川明董广巧华侨大学计算机科学与技术学院福建厦门361021

在服务互联网环境下,服务供应链正在发展成为具有复杂、动态、协同交互特征的服务生态系统网络。针对由众多第三方服务平台、服务提供商和服务消费者组成的服务生态系统的动态演化问题,首先构建了其基于Logistic模型的共生演化模型,并... 详细信息

在服务互联网环境下,服务供应链正在发展成为具有复杂、动态、协同交互特征的服务生态系统网络。针对由众多第三方服务平台、服务提供商和服务消费者组成的服务生态系统的动态演化问题,首先构建了其基于Logistic模型的共生演化模型,并采用雅各比矩阵研究了不同价值共创单元在服务生态系统中保持稳定状态的共生模式和共生条件。最后,通过数值仿真分析了共生作用系数、市场规模容量等相关因素对其共生演化的影响。仿真结果显示:服务生态系统价值共创单元的共生演化和共生作用系数与其共生模式紧密相关。在互利互惠的共生模式下,服务生态系统价值共创单元的最终稳定规模最大,是其共生演化的最佳方向;服务生态系统价值共创单元的共生演化稳定点与其自然增长率、初始规模无关,与市场容量规模有关,各类价值共创单元的市场容量规模越大,则稳定规模值越大。

关键词：服务生态系统价值共创单元共生演化模型稳定状态

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不同硬化模型对铝合金板冲压成形模拟结果的影响

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机械工程学报 2017年第22期53卷 57-66页

作者：金飞翔钟志平李凤娇孟辉机械科学研究总院北京100044 北京机电研究所北京100083 长城汽车股份有限公司哈佛技术中心保定071000

研究不同塑性变形硬化模型对汽车5182-O铝合金板材冲压成形模拟结果的影响。采用材料单向拉伸试验得到应力应变关系曲线,基于Hollomom、Krupskowsky与Power方程对曲线进行拟合,建立材料室温下塑性变形硬化模型,对厚度为1.5 mm和0.85 mm... 详细信息

研究不同塑性变形硬化模型对汽车5182-O铝合金板材冲压成形模拟结果的影响。采用材料单向拉伸试验得到应力应变关系曲线,基于Hollomom、Krupskowsky与Power方程对曲线进行拟合,建立材料室温下塑性变形硬化模型,对厚度为1.5 mm和0.85 mm的5182板材进行冲压试验和有限元模拟分析,对比分析冲压试验与模拟结果。试验与模拟结果显示,当板料厚度为1.5 mm时,板料冲压试验的成形力最大为42.95 k N,板料拉深深度为30.58 mm,基于Power方程计算得到的最大成形力为41.5k N与试验结果比较接近,Hollomom方程计算得到的拉深深度为30.546 mm,板材成形厚度分布与试验结果比较接近;当板料厚度为0.85 mm时,板料冲压试验的成形力最大为34.47k N,板料拉深深度为33.792 mm,基于Power方程计算得到的最大成形力为34.27 k N与试验结果比较接近,Hollomom方程计算得到的拉深深度为33.636 mm,板材成形厚度分布与试验结果比较接近。基于三种硬化模型铝合金冲压成形过程的计算模拟分析结果,并通过与试验对比得到不同硬化模型对铝合金板材冲压成形计算模拟的影响,进一步为汽车铝合金覆盖件在成形工艺的研究分析提供理论指导。

关键词：铝合金硬化模型冲压成形有限元模拟

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