检索结果-南通市图书馆

Effects of Soil Texture on the Growth of Young Apple Trees and Soil Microbial Community Structure Under Replanted Conditions

在线全文

学校读者我要写书评

暂无评论

Horticultural Plant Journal 2020年第3期6卷 123-131页

作者： Yuefan Sheng Haiyan Wang MeiWang Hanhao Li Li Xiang Fengbing Pan Xuesen Chen Xiang Shen Chengmiao Yin Zhiquan Mao College of Horticultural Science and Engineering/State Key Laboratory of Crop Biology Shandong Agricultural UniversityTai’anShandong 271018China College of Resources and Environment Shandong Agricultural UniversityNational Engineering Laboratory for Efficient Utilization of Soil and Fertilizer ResourcesTai’anShandong 271018China

A two-year field experiment was carried out in order to study the occurrence degree and mechanism of apple replant disease(ARD)in the apple orchards with different soil textures.So we can adopt appropriate controlmeasures according to the severity of ARD.Healthy two-year-old seedlings with consistent growth were selected,of which the root stock was T337 and the scion was Yanfu 3.There were significant differences in biomass between methyl bromide fumigation and replanted treatments,and the difference was the largest in clay loam,followed by sandy loam,and loam,which verified ARD in clay loam was most serious,followed by sandy loam and loam.Based on high-throughput sequencing of fungi in soil samples,fungal richness and diversity were the highest in clay loam,followed by sandy loam,and loam.The relative abundance of Fusarium in SX,SL,FX,FL,WX and WL was 7.33%,19.32%,2.70%,4.24%,10.71%and 23.87%,respectively.Based on Real-time quantitative analysis,there were significant differences in the number of Fusarium oxysporum and Fusarium solani between methyl bromide fumigation and replanted treatments,i.e.,clay loam>sandy loam>loam.Fusarium was the main pathogen causing ARD.This shows that ARD is the most serious under replanted clay loam condition.High-throughput sequencing technology was used to prove the difference in Fusarium was one of the important reasons for ARD under different soil textures.This technology provides a new idea for the prevention and control of ARD.

关键词： Apple Apple replant disease Fungi community structure Soil texture High-throughput sequencing

Analysis of the Fungal Community in Apple Replanted Soil Around Bohai Gulf

在线全文

学校读者我要写书评

暂无评论

Horticultural Plant Journal 2018年第5期4卷 175-181页

作者： WANG Gongshuai YIN Chengmiao pan fengbing WANG Xiaobao XIANG Li WANG Yanfang WANG Jinzheng TIAN Changping CHEN Jie MAO Zhiquan State Key Laboratory of Crop Biology/College of Horticultural Science and Engineering Shandong Agricultural University College of Chemistry and Material Science Shandong Agricultural University Shandong Instiute of Pomology Yantai Academy of Agricultural & Science Garden Office of Linyi Lanshan

Apple replant disease(ARD) is a frequently occurring plant disease in replanted orchards around Bohai Gulf, which causes growth inhibition and even death of plants. The aim of this study was to investigate the etiology of ARD around Bohai Gulf. In this study, the primary growth inhibition of apple seedlings was evaluated in ten replanted soils, sampled around Bohai Gulf. A fungal clone library was used to identify changes in the structure and composition of the soil fungal community. The results revealed that the Simpson diversity indices of Laizhou and Pulandian orchards were higher than others, presenting severe ARD. Ascomycota dominated around Bohai Gulf at the phyla level. Fusarium and Saccharomyces were abundant in all replanted soils. In addition, correlations between the relative abundance of fungal genera in soils and the severity of ARD were analyzed. The results showed that Fusarium was correlated positively with the severity of ARD, but Mortierella was negatively correlated. Furthermore, the quantitative PCR of Fusarium oxysporum, which was regarded as a factor of ARD, was performed. Overall, this study demonstrated that ARD was strongly associated with an unbalanced microbial ecosystem with more pathogenic fungi, while Fusarium in the apple replanted soil was the key factor for ARD around Bohai Gulf.

关键词： apple replanted disease Fungi clone library fusarium fungal community

不同蛋白发酵物对连作土壤微生物环境和平邑甜茶幼苗生长的影响

在线全文

学校读者我要写书评

暂无评论

园艺学报 2022年第2期49卷 395-406页

作者：潘凤兵陈冉姜伟涛王海燕吕毅沈向陈学森尹承苗毛志泉山东农业大学园艺科学与工程学院作物生物学国家重点实验室山东泰安271018 威海市农业科学院山东威海264200

为探讨不同物料蛋白发酵物防控苹果连作障碍的可行性,以盆栽平邑甜茶为试材,设连作土(对照)、灭菌土(高标对照)和分别在连作土中添加蚯蚓发酵物、大豆粕发酵物、杂鱼发酵物等5个处理,测定土壤微生物总量、有害真菌数量、土壤酶活性、土... 详细信息

为探讨不同物料蛋白发酵物防控苹果连作障碍的可行性,以盆栽平邑甜茶为试材,设连作土(对照)、灭菌土(高标对照)和分别在连作土中添加蚯蚓发酵物、大豆粕发酵物、杂鱼发酵物等5个处理,测定土壤微生物总量、有害真菌数量、土壤酶活性、土壤酚酸类物质含量、平邑甜茶生物量和植株根系相关指标。结果表明添加3种不同蛋白发酵物均能有效提高连作土壤细菌数量,降低真菌数量,改变了连作土壤中的真菌群落结构,其中添加蚯蚓发酵物降低有害真菌效果最好,尖孢镰孢菌、层出镰孢菌、腐皮镰孢菌和串珠镰孢菌分别比连作土(对照)降低了59.2%、52.0%、64.9%和67.1%。3种发酵物也不同程度地提高了连作土壤中的土壤酶活性,降低了土壤酚酸类物质含量。蚯蚓发酵物处理的平邑甜茶幼苗生物量明显高于连作土(对照),植株干质量比对照提高了90.22%。发酵物也促进了植株根系的生长、根抗氧化酶活性和呼吸速率的提高,效果最为显著的蚯蚓发酵物,根系的总根长、表面积、根体积和根尖数分别比连作土(对照)提高了147.21%、225.87%、299.85%和291.03%,SOD、POD、CAT和根系呼吸速率分别比连作土(对照)提高了46.90%、133.94%、197.69%和113.23%。综上,不同物料蛋白的发酵物均能通过调节微生物群落结构、改善连作土壤环境、促进植株生长等途径不同程度地缓解苹果连作障碍对幼树造成的危害,综合各项指标来看,蚯蚓发酵的效果最为显著。

关键词：苹果连作障碍蛋白质发酵土壤生物环境

LTB245E1-BLK222型断路器机构缺陷的分析

在线全文

学校读者我要写书评

暂无评论

四川电力技术 2019年第1期42卷 68-71页

作者：熊攀王嘉易李辉李逢兵贾志杰董汉彬国网泸州供电公司四川泸州646002 国网四川省电力公司电力科学研究院四川成都610041 国网眉山供电公司四川眉山620010

LTB245E1型断路器为220 kV SF_6断路器,配置BLK222型弹簧操动机构,目前广泛应用于四川电网中。通过对一起该型断路器弹簧机构缺陷原因的分析,就LTB245E1-BLK222型断路器检修工作重点及对策进行了探讨,以预防电气故障的发生。

关键词：断路器缺陷弹簧机构检修工作

葱、芥菜和小麦轮作对老龄苹果园土壤环境的影响

在线全文

学校读者我要写书评

暂无评论

园艺学报 2019年第11期46卷 2224-2238页

作者：王海燕盛月凡李前进王玫潘凤兵陈学森沈向尹承苗毛志泉山东农业大学园艺科学与工程学院作物生物学国家重点试验室山东泰安271018 洛川县苹果生产技术开发办公室陕西洛川727400

在山东省烟台市大沙岭、凤毛寨和湾头村等的25年生老苹果园中,在1年内进行葱、芥菜和小麦轮作,然后收集每个果园连作土壤和轮作后的土壤,并以溴甲烷熏蒸处理的连作土壤为高标对照,在盆栽试验条件下研究各处理土壤对平邑甜茶(苹果砧木)... 详细信息

在山东省烟台市大沙岭、凤毛寨和湾头村等的25年生老苹果园中,在1年内进行葱、芥菜和小麦轮作,然后收集每个果园连作土壤和轮作后的土壤,并以溴甲烷熏蒸处理的连作土壤为高标对照,在盆栽试验条件下研究各处理土壤对平邑甜茶(苹果砧木)幼苗生长和土壤环境的影响。结果表明:与连作土壤相比,在3种植物轮作以后的土壤中,平邑甜茶幼苗的生物量显著提高,且地下部干、鲜质量可提高1~2倍;根系呼吸速率增长均超过1倍;同时,3个果园的轮作土壤中细菌数量分别增加148.47%、198.57%和165.44%,真菌降低45%左右,使得细菌/真菌比值提高了4倍;土壤酶活性提高,酚酸类物质含量大幅降低,根皮苷含量分别降低86.67%、46.12%和86.31%;此外,尖孢镰孢菌的拷贝数分别降低76.34%、49.22%和70.89%。

关键词：苹果轮作土壤平邑甜茶生物量微生物酚酸

多学科联合早期康复对ICU肺炎合并营养不良老年患者的干预效果

在线全文

学校读者我要写书评

暂无评论

中外医学研究 2022年第5期20卷 156-158页

作者：窦微微亢凤兵白娜娜潘维花郝爱慈钮静申青燕张梦蕊北京市大兴区中西医结合医院北京市大兴区康复医院北京100076

目的:研究多学科联合进行早期康复对肺炎合并营养不良老年患者的营养状态、呼吸支持及精神状态的影响。方法:选取2017年1月-2020年1月北京市大兴区中西医结合医院诊治的100例肺炎合并营养不良老年患者,按照随机数表法将其分为对照组和... 详细信息

目的:研究多学科联合进行早期康复对肺炎合并营养不良老年患者的营养状态、呼吸支持及精神状态的影响。方法:选取2017年1月-2020年1月北京市大兴区中西医结合医院诊治的100例肺炎合并营养不良老年患者,按照随机数表法将其分为对照组和观察组,每组50例。对照组采用传统康复治疗,观察组采用多学科联合早期康复治疗。比较两组的营养状态、呼吸支持及精神状态。结果:治疗后,两组总蛋白、白蛋白、前白蛋白、血红蛋白水平均高于治疗前,且观察组均高于对照组,差异均有统计学意义(P<0.05)。两组腹胀或腹泻、机械通气、谵妄发生率比较,差异均有统计学意义(P<0.05)。结论:多学科联合早期康复能有效改善营养状态、减少呼吸支持、降低谵妄发生。

关键词：多学科联合早期康复肺炎营养不良机械通气谵妄