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Journal of Northeast Agricultural University(English Edition) 2016年第1期23卷 44-49页

作者： Huo feng Li Ning Lin xiao-Li Institute of Veterinary Drug and Food Control Harbin 150010 China

A method was developed for the simultaneous determination of four kinds of estrogens （hexoestrol, diethylstilbestrol, estrone, and 17-beta-estradiol） in feed by gas chromatography-mass spectrometry （GC-MS）. After the sample was extracted by ethyl ether and cleaned-up on HLB phase extraction column, four kinds of estrogens were derived and quantified in gas chromatographymass spectrometry. The results showed that the linear detectable ranged from 2.5 ng· mL-1 to 250 ng· mL-1for hexoestrol and from 5 ng· mL-1 to 500 ng· mL-1 for three other estrogens with the correlation coefficients （R2） were no less than 0.990. The recoveries were in the range of 76.34%-96.33% and the relative standard deviation was no more than 22.7%. The limits of quantitation （LOQ） for all analytics were between 10 ug· kg^-1 and 20 ug· kg^-1. The method was accurate and sensitive and could meet the actual requirements for the analyses of feed samples.

关键词： gas chromatography-mass spectrometry hexoestrol diethylstilbestrol estrone 17-beta-estradiol feed

TGF-β1-regulated miR-3691-3p targets E2F3 and PRDM1 to inhibit prostate cancer progression

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Asian Journal of Andrology 2021年第2期23卷 188-196页

作者： Yue-Mei Hu xiao-Li Lou Bao-Zhu Liu Li Sun Shan Wan Lei Wu Xin Zhao Qing Zhou Mao-Min Sun Kun Tao Yong-Sheng Zhang shou-Li Wang Department of Pathology School of Biology and Basic Medical SciencesSoochow UniversitySuzhou 215123China Department of Pathology Tongren HospitalShanghai Jiao Tong University School of MedicineShanghai 200336China Collaborative Innovation Center of Clinical Immunology between Soochow University and Sihong People's Hospital Sihong 223900China Department of Pathology Sihong People's HospitalSihong 223900China Laboratory Animal Research Center Soochow University School of MedicineSuzhou 215123China Department of Surgery The First Affiliated Hospital of Soochow UniversitySuzhou 215006China Department of Pathology The Second Affiliated Hospital of Soochow UniversitySuzhou 215004China Suzhou Key Laboratory of Tumor Microenvironment and Pathology Soochow UniversitySuzhou 215006China

Transforming growth factor-β1(TGF-β1)acts as a tumor promoter in advanced prostate cancer(PCa).We speculated that microRNAs(miRNAs)that are inhibited by TGF-β1 might exert anti-tumor *** assess this,we identified several miRNAs downregulated by TGF-β1 in PCa cell lines and selected miR-3691-3p for detailed analysis as a candidate anti-oncogene ***-3691-3p was expressed at significantly lower levels in human PCa tissue compared with paired benign prostatic hyperplasia tissue,and its expression level correlated inversely with aggressive clinical pathological *** of miR-3691-3p in PCa cell lines inhibited proliferation,migration,and invasion,and promoted *** miR-3691-3p target genes E2F transcription factor 3(E2F3)and PR domain containing 1,with ZNF domain(PRDM1)were upregulated in miR-3691-3p-overexpressing PCa cells,and silencing of E2F3 or PRDM1 suppressed PCa cell proliferation,migration,and *** of mice bearing PCa xenografts with a miR-3691-3p agomir inhibited tumor growth and promoted tumor cell *** with the negative regulation of E2F3 and PRDM1 by miR-3691-3p,both proteins were overexpressed in clinical PCa specimens compared with noncancerous prostate *** results indicate that TGF-β1-regulated miR-3691-3p acts as an anti-oncogene in PCa by downregulating E2F3 and *** results provide novel insights into the mechanisms by which TGF-β1 contributes to the progression of PCa.

关键词： E2F transcription factor 3 miR-3691-3p PR domain containing 1,with ZNF domain prostate cancer transforming growth factor-β1

Eye metastasis in lung adenocarcinoma mimicking anterior scleritis:A case report

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World Journal of Clinical Cases 2020年第2期8卷 410-414页

作者： Hua-Fei Chen Wen-Xian Wang xiao-feng Li Li-Xin Wu You-Cai Zhu Kai-Qi Du Chun-Wei Xu Thoracic Disease Center Zhejiang Rongjun HospitalJiaxing 314000Zhejiang ProvinceChina Department of Chemotherapy Zhejiang Cancer HospitalHangzhou 310022Zhejiang ProvinceChina Department of Pathology Fujian Cancer HospitalFujian Medical University Cancer HospitalFuzhou 350014Fujian ProvinceChina

BACKGROUND The eye is a rare site for lung cancer metastasis. Indeed, ocular metastasis is one of the greatest challenges to quality of life in a cancer patient. Here we present a patient with lung adenocarcinoma and ocular *** SUMMARY The patient was a 70-year-old man diagnosed with lung adenocarcinoma who developed eye metastasis mimicking anterior scleritis. Brain magnetic resonance imaging showed an abnormal signal in the right eye. Based on next generation sequencing of the surgical specimen, the patient was shown to have a KRAS point mutation(p.G12D).CONCLUSION Multidiscipline expertise collaboration is needed to make the early diagnosis and determine the prompt treatment in patients. We hope to increase the awareness of the possibility of lung cancer metastasizing to the eye.

关键词： Eye Metastases Non-small cell lung cancer KRAS mutation Case report

Online residual useful life prediction of large-size slewing bearings A data fusion method

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Journal of Central South University 2017年第1期24卷 114-126页

作者：封杨黄筱调洪荣晶陈捷 Jiangsu Key Laboratory of Digital Manufacturing for Industrial Equipment and Control Technology(School of Mechanical and Power Engineering) Nanjing Tech UniversityNanjing 210009China

To decrease breakdown time and improve machine operation reliability,accurate residual useful life(RUL) prediction has been playing a critical role in condition based monitoring.A data fusion method was proposed to achieve online RUL prediction of slewing bearings,which consisted of a reliability based RUL prediction model and a data driven failure rate(FR) estimation ***,an RUL prediction model was developed based on modified Weibull distribution to build the relationship between RUL and ***,principal component analysis(PCA) was introduced to process multi-dimensional life-cycle vibration signals,and continuous squared prediction error(CSPE) and its time-domain features were employed as equipment performance degradation ***,an FR estimation model was established on basis of the degradation features and relevant FRs using simplified fuzzy adaptive resonance theory map(SFAM) neural ***,real-time FR of equipment can be obtained through FR estimation model,and then accurate RUL can be calculated through the RUL prediction *** of a slewing bearing life test show that CSPE is an effective indicator of performance degradation process of slewing bearings,and that by combining actual load condition and real-time monitored data,the calculation time is reduced by 87.3%and the accuracy is increased by 0.11%,which provides a potential for online RUL prediction of slewing bearings and other various machineries.

关键词： slewing bearing life prediction Weibull distribution failure rate estimation data fusion

无线传感器网络中基于位置信息的路由算法研究

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计算机工程与设计 2010年第22期31卷 4765-4768,4801页

作者：侯守峰周小佳闫斌电子科技大学自动化工程学院四川成都611731

为了保证移动无线传感器网络中数据的高效稳定传输,提出了一种基于地理位置信息支持多个移动sink的路由算法。该算法将监测区域分成一个固定不变的规则网格网络,并对网格簇头节点的选举方法进行了改进;通过数据的冗余传递和搜索框、候... 详细信息

为了保证移动无线传感器网络中数据的高效稳定传输,提出了一种基于地理位置信息支持多个移动sink的路由算法。该算法将监测区域分成一个固定不变的规则网格网络,并对网格簇头节点的选举方法进行了改进;通过数据的冗余传递和搜索框、候选转发节点集合的设置保证了数据传送的可靠性与时延性;同时容忍圆的引入保证了低功耗的要求。仿真结果表明,该算法较两层数据分发协议有更高的传输可靠性和较低的功耗。

关键词：无线传感器网络容忍圆搜索框网格路由候选转发节点集合坐标系集合

长期培养和宏组学分析揭示了烃污染环境中的甲基营养型产甲烷途径

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Engineering 2023年第5期24卷 264-275,I0007页

作者： Yi-Fan Liu Jing Chen Zhong-Lin Liu Zhao-Wei Hou Bo Liang Li-Ying Wang Lei Zhou Li-Bin shou Dan-Dan Lin Shi-Zhong Yang Jin-feng Liu xiao-Lin Wu Ji-Dong Gu Bo-Zhong Mu State Key Laboratory of Bioreactor Engineering and School of Chemistry and Molecular Engineering East China University of Science and TechnologyShanghai 200237China Exploration and Development Research Institute of Daqing Oilfield Company Limited PetroChinaDaqing 163712China Environmental Engineering and Engineering Group Guangdong Technion Israel Institute of TechnologyShantou 515063China Shanghai Institute of Pollution Control and Ecological Security Shanghai 200092China Engineering Research Center of Microbial Enhanced Oil Recovery(MEOR) East China University of Science and TechnologyShanghai 200237China

针对石油烃污染的厌氧环境,利用微生物的代谢作用将石油烃转化为甲烷是一种潜在的生物修复策略。然而,目前对于微生物厌氧烃降解产甲烷的机制尚不清楚。经过十年的持续富集和转接培养,我们获得了一种能够降解正构烷烃(C_(15)~C_(20))并... 详细信息

针对石油烃污染的厌氧环境,利用微生物的代谢作用将石油烃转化为甲烷是一种潜在的生物修复策略。然而,目前对于微生物厌氧烃降解产甲烷的机制尚不清楚。经过十年的持续富集和转接培养,我们获得了一种能够降解正构烷烃(C_(15)~C_(20))并且产甲烷富集培养体系,并且经过转接培养该培养物在甲烷生产的动力学特性方面得到了持续的提高。综合宏基因组和宏转录组的分析结果,发现正构烷烃主要通过Desulfosarcinaceae、Firmicutes和Synergistetes菌通过富马酸加成的方式进行起始活化,然后在Tepidiphilus菌的协同作用下进一步降解。同时,体系中含量较多的Anaerolineaceae菌主要负责死细胞生物质的回收。根据宏转录组学分析结果,甲烷主要通过H2依赖型的甲基营养甲烷途径产生,主要由候选门Verstraetearchaeta内的Methanomethyliaceae通过利用死细胞生物质回收代谢过程中产生的三甲胺来产生甲烷。这些发现表明,依赖H2的甲基营养产甲烷菌以及甲基营养产甲烷菌可能在含石油烃的地下生态系统的碳循环过程中发挥着重要的作用。

关键词：产甲烷菌石油烃污染富集培养正构烷烃代谢作用厌氧环境宏基因组修复策略

Detection of H pylori antibody profile in serum by protein array

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World Journal of Gastroenterology 2006年第25期12卷 4044-4048页

作者： feng-Chan Han Xu-Jun Li Hong Jiang Li-Peng Qin Ding Li Yan-Hai Guo Zhi-Guang Liu Li Zhang xiao-Jun Yan Institute of Genetic Diagnosis State Key Laboratory of Cancer Biology The Fourth Military Medical University Xi'an 710032 Shaanxi Province China Department of Gastroenterology Xi'an Central Hospital Xi'an 710032 Shaanxi Province China

AIM： To detect multiple H pylori antibodies in serum samples of individuals who carryHpylori by protein array. METHODS： Recombinant H pylori antigens, urease B subunit （UreB）, vacuolating toxin A （VacA） and cytotoxin associated gene A protein （CagA）, were prepared and immobilized in matrixes on nitrocellulose membrane by robotics to bind the specific immunoglobulin G （IgG） antibodies in serum. Staphylococcus protein A （SPA） labeled by colloid gold was used to integrate the immuno-complex and gave red color signal. The scanner based on charge-coupled device （CCD） could collect the image signal and convert it into digital signal. RESULTS： When human IgG was printed on the membrane in increasing concentrations and incubated with immunogold, a linear dose response curve was obtained and the detection limit for IgG was about 0.025 ng. The cutoff values, which were defined as the mean grey level plus 3 times of standard deviation, were 27.183, 28.546 and 27.402, for anti-UreB IgG, anti- CagA IgG and anti-VacA IgG, respectively, as 400 human serum samples with negative H pylori antibodies were detected by the protein array. When 180 serum samples from patients in hospital were employed for detection of IgG against UreB, CagA and VacA, the sensitivity of the protein array was 93.4%, 95.4%, 96.0%, and the specificity was 94.8%, 94.4% and 97.5%, respectively, as compared with the results obtained by ELISA. The assay also showed high reproducibility, uniformity and stability, and the results were available within 30 min. CONCLUSION： The protein array is a very practical method for rapid detection of multiple antibodies in serum samples. It is especially useful for large scale epidemiological investigation of the infection of Hpylori.

关键词： Helicobacter pylori Protein array Antibody Immunogold

Outline of Ulcerative Colitis Treated by Acupuncture: A Review

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Journal of Acupuncture and Tuina Science 2003年第2期1卷 11-13页

作者： HUANG Qin-feng xiao Yuan-chun Shanghai Research Institute of Acupuncture and Meridian 200030 China

To analyze 73 theses that all accorded with statistical demand about ulcerative colonitis. The statistics found that the commonly used therapies were acupuncture, moxibustion, acupuncture-moxibustion, herb-partitioned moxibustion, acupoint-injection, point-radiation therapy, auricular-plaster therapy, catgut implantation at acupoint, point application etc. The commonly used points were Zusanii (ST36), Tianshu (ST25), Pishu (BL20), Weishu (BL21), Zhongwan (CV12), Dachangshu (BL25) etc. The total effective rate in clinic was 95.40%. Key Words Colonitis - Ulcerative - Acupuncture-moxi-bustion - Review Literature Author: HUANG Qin-feng (1951-), female, associate professorTranslator: xiao Yuan-chun

关键词： Colonitis Ulcerative Acupuncture-moxi-bustion Review Literature

Independent prognostic value of lipocalin-2 in congenital heart disease-associated pulmonary artery hypertension

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World Journal of Cardiology 2024年第12期16卷 720-730页

作者： Zhang-Ke Guo Ping-Gui Chen Yao-Xuan Li Hong Jiao xiao-Hui Kong Song Bai xiao-feng Li Ai-Jun Liu Guo-Liang Wang Department of Cardiac Surgery Beijing Children’s HospitalCapital Medical UniversityNational Center for Children's HealthBeijing 100045China Department of General Surgery The Third Hospital of Shanxi Medical UniversityShanxi Bethune HospitalShanxi Academy of Medical SciencesTongji Shanxi HospitalTaiyuan 030032Shanxi ProvinceChina Department of Health Statistics School of Public HealthShanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina Department of Tumor and Immunology Beijing Pediatric Research InstituteBeijing Children’s HospitalCapital Medical UniversityNational Center for Children's HealthBeijing 100045China Department of Pediatric Cardiac Surgery Beijing Anzhen HospitalCapital Medical UniversityBeijing Institute of Heart Lung and Blood Vessel DiseasesBeijing 100029China

BACKGROUND Timely and accurate evaluation of the patient's pulmonary arterial pressure(PAP)is of great significance for the treatment of congenital heart ***,there is no non-invasive gold standard method for evaluating *** To assess the prognostic value of lipocalin-2(LCN2)in relation to PAP in patients with congenital heart disease associated with pulmonary artery *** We conducted a retrospective analysis of 69 pediatric patients diagnosed with ventricular septal *** patients’clinical and laboratory data were *** serum LCN2 concentrations were compared between the pulmonary arterial hypertension(PAH)group and the nonPAH *** correlation of LCN2 concentration with PAH classification was evaluated using binary logistic regression *** receiver operating characteristic(ROC)curve was used to evaluate the diagnostic potential of LCN2 for *** Serum LCN2 concentration significantly correlated with patients’mean PAP(r=0.544,P<0.001),but not correlated with creatinine(P=0.446)or blood urea nitrogen(P=0.747).LCN2 levels were significantly correlated with PAH in both univariate[odds ratio(OR)1.107,95%CI:1.033-1.185,P=0.004)]and multivariate regression analysis(OR 1.150,95%CI:1.027-1.288,P=0.015).ROC curve analysis revealed an area under the curve of 0.783 for *** the cutoff value of 19.42 ng/mL,the sensitivity and specificity of LCN2 for diagnosing PAH is 90.19%and 55.56%,***2 concentration also significantly correlated with the post-repair mean PAP in patients with congenital heart disease(r=0.532,P=0.009).CONCLUSION LCN2 is emerging as a candidate biomarker for assessing PAP in patients with congenital heart *** high sensitivity in diagnosing PAH makes it a valuable tool in patient management.

关键词： Congenital heart disease Pulmonary arterial hypertension Lipocalin-2 Endothelin-1 Biomarker

Ultra-low-power backscatter-based software-defined radio for intelligent and simplified IoT network

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Frontiers of Information Technology & Electronic Engineering 2022年第1期23卷 19-30页

作者： Huixin DONG Wei KUANG Fei xiao Lihai LIU feng XIANG Wei WANG Jianhua HE School of Electronic Information and Communication Huazhong University of Science and TechnologyWuhan430074China School of Management Huazhong University of Science and TechnologyWuhan430074China China Railway Siyuan Survey and Design Group Co. Ltd.Wuhan430063China Wuhan Maritime Communication Research Institute Wuhan430079China School of Computer Science and Electronic Engineering University of EssexColchesterCO43SQUK

The recent decade has witnessed an upsurge in the demands of intelligent and simplified Internet of Things(IoT)networks that provide ultra-low-power communication for numerous miniaturized *** the research community has paid great attention to wireless protocol designs for these networks,researchers are handicapped by the lack of an energy-efficient software-defined radio(SDR)platform for fast implementation and experimental *** SDRs perform well in battery-equipped systems,but fail to support miniaturized IoT devices with stringent hardware and power *** paper takes the first step toward designing an ultra-low-power SDR that satisfies the ultra-low-power or even battery-free requirements of intelligent and simplified IoT *** achieve this goal,the core technique is the effective integration ofµW-level backscatter in our SDR to sidestep power-hungry active radio frequency *** carefully develop a novel circuit design for efficient energy harvesting and power control,and devise a competent solution for eliminating the harmonic and mirror frequencies caused by backscatter *** evaluate the proposed SDR using different modulation schemes,and it achieves a high data rate of 100 kb/s with power consumption less than 200µW in the active mode and as low as 10µW in the sleep *** also conduct a case study of railway inspection using our platform,achieving 1 kb/s battery-free data delivery to the monitoring unmanned aerial vehicle at a distance of 50 m in a real-world environment,and provide two case studies on smart factories and logistic distribution to explore the application of our platform.

关键词： Backscatter Ultra-low-power SDR IoT networks