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优质高分子量麦谷蛋白亚基1Dx5+1Dy10的分子检测

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麦类作物学报 2008年第3期28卷 425-429页

作者：刘东涛陈荣振刘世来王来花李德民张会云王静冯国华江苏徐淮地区徐州农业科学研究所江苏徐州221121

为给优质强筋小麦的选育提供高效、快速、稳定的选择手段,以已知高分子量麦谷蛋白亚基组成的10个小麦品种为材料,比较了5个已报道的高分子量麦谷蛋白亚基1Dx5(1Dy10)的PCR分子标记,并对12个DNA模板进行了分子检测。结果表明,在5个分子... 详细信息

为给优质强筋小麦的选育提供高效、快速、稳定的选择手段,以已知高分子量麦谷蛋白亚基组成的10个小麦品种为材料,比较了5个已报道的高分子量麦谷蛋白亚基1Dx5(1Dy10)的PCR分子标记,并对12个DNA模板进行了分子检测。结果表明,在5个分子标记中,有1个分子标记是非特异的,有2个分子标记是显性的特异标记,另2个则是共显性的分子标记。其中,由引物Dx-F、Dx5-F和Dx-R组成的共显性标记,能稳定地扩增出5亚基和非5亚基的特异序列,并且无非特异PCR产物,是用于高分子量麦谷蛋白亚基1Dx5分子检测的最理想标记。

关键词：小麦高分子量麦谷蛋白亚基(HMW-GS) PCR 分子标记

Precise determination of the pole position of the exotic Z_(c)(3900)

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Science China(Physics,Mechanics & Astronomy) 2024年第9期67卷 84-90页

作者： Yun-hua Chen Meng-Lin Du feng-Kun guo School of Mathematics and Physics University of Science and Technology BeijingBeijing 100083China School of Physics University of Electronic Science and Technology of ChinaChengdu 611731China CAS Key Laboratory of Theoretical Physics Institute of Theoretical PhysicsChinese Academy of SciencesBeijing 100190China School of Physical Sciences University of Chinese Academy of SciencesBeijing 100049China Peng huanwu Collaborative Center for Research and Education Beihang UniversityBeijing 100191China

We perform a unified description of the experimental data of the π^(+)π^(-)and J/ψπ^(±)invariant mass spectra for e^(+)e^(-)→J/ψπ^(+)π^(-)and the D^(0)D^(*-)mass spectrum for e^(+)e^(-)→D^(0)D^(*-)π^(+)at e^(+)e^(-)center-of-mass energies 4.23 and 4.26 *** analysis takes into account open-charm meson loops that contain triangle singularities,the J/ψπ-DD^(*)coupled-channel interaction respecting unitarity,and the strong ππ-KK final state interaction using dispersion *** analysis leads to a precise determination of the Z_(c)(3900)pole with the pole mass and width(3880.7±1.7_(stat)±22.4_(syst))MeV and(35.9±1.4_(stat)±15.3_(syst))MeV,respectively,and hints at that the DD^(*)molecular and non-molecular components are of similar importance in the Z_(c)(3900)formation.

关键词： exotic hadrons dispersion relations final state interaction triangle singularity

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内切纤维素酶改善ONP浆滤水性研究及应用

造纸科学与技术 2018年第4期37卷 62-66,87页

作者：李强冯国华曾文榄宋德军广东溢多利生物科技股份有限公司广东珠海519060

探讨了内切纤维素酶TM3性能及改善ONP浆料滤水性的应用工艺。结果表明:内切纤维素酶TM3的最适温度为50℃,最适p H为7.0,在p H6.0~8.0范围内有良好的酶活特性。内切纤维素酶TM3改善ONP浆料滤水性的最佳工艺:预处理时间为60 min,浆料浓度3... 详细信息

探讨了内切纤维素酶TM3性能及改善ONP浆料滤水性的应用工艺。结果表明:内切纤维素酶TM3的最适温度为50℃,最适p H为7.0,在p H6.0~8.0范围内有良好的酶活特性。内切纤维素酶TM3改善ONP浆料滤水性的最佳工艺:预处理时间为60 min,浆料浓度3.0%,用量为0.05μ/g(绝干浆)。与常规工艺相比,经内切纤维素酶TM3处理ONP浆打浆度下降2.0°SR,成纸环压指数、耐折度有所提高,底驱网辊电流降低0.91%。

关键词：内切纤维素酶 ONP浆滤水

云南省盈江县中缅边境恶性疟原虫对氯喹、哌喹体外敏感性研究

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中国地方病防治 2016年第8期31卷 858-859页

作者：张艳梅冯国华李鸾娇王颖娜杨照青昆明医科大学第一附属医院云南昆明650032 昆明医科大学病原生物学与免疫学系昆明医科大学生物工程中心云南省德宏洲盈江县平原镇卫生院

目的监测云南省盈江县中缅边境恶性疟原虫对氯喹、哌喹的体外敏感性，了解两种抗疟药有无交叉耐药。方法选取2007—2009年当地病人体内的恶性疟原虫，体外培养成活共28株，在体外进行对氯喹、哌喹的半效抑虫浓度（IC50）值测定；并对疟... 详细信息

目的监测云南省盈江县中缅边境恶性疟原虫对氯喹、哌喹的体外敏感性，了解两种抗疟药有无交叉耐药。方法选取2007—2009年当地病人体内的恶性疟原虫，体外培养成活共28株，在体外进行对氯喹、哌喹的半效抑虫浓度（IC50）值测定；并对疟原虫的Pfert基因片段扩增，测序，比对。结果28株恶性疟原虫株体外氯喹的IC50值为（463．9±256．0）nm；哌喹的IC50值为（9．0±6．7nm）和野生株3D7相比，均有显著性差异（P〈0.05）。28株（100％）恶性疟原虫对氯喹均耐药株；5／28（16．1％）的疟原虫对哌喹耐药；7／28（21．4％）的疟原虫对哌喹敏感。28株（100％）疟原虫的Pfert基因发现均有和氯喹耐药相关的76T变异。应用Pearson线性相关分析发现CQ和PPQ间存在又抗药性（P〈0．001）。结论云南省盈江县中缅边境的恶性疟原虫对氯喹、哌喹耐药的情况严峻，尤其是氯喹。

关键词：恶性疟原虫氯喹哌喹体外敏感试验 Pfert

Effects of connection types and elevated temperature on the impact behaviour of restrained beam in portal steel frame

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Defence Technology（防务技术） 2023年第7期25卷 174-191页

作者： Yu-Xu guo feng Xi Ying-hua Tan feng Liu Ya-Chao Hu School of Civil Engineering Shandong Jianzhu UniversityJinan250101PR China Shandong Provincial Key Laboratory of Civil Engineering Disaster Prevention and Mitigation Shandong University of Science and TechnologyQingdao266590PR China Key Laboratory of Building Structural Retrofitting and Underground Space Engineering Shandong Jianzhu UniversityJinan250101PR China

Based on the background of structural protection and Disaster Reduction Engineering, the dynamic behaviour and failure mechanism of restrained beams in portal steel frames in localised fire are investigated via experimental measurement and numerical simulation techniques. Comprehensive parametric studies are carried out to discuss the influence of end connection types, temperature, impact velocity,impact mass and span-to-depth ratio(SDR) on the dynamic response of the beams. The characteristics of deformation, internal force and energy distribution about the restrained beams and its joints are investigated. A temperature dependent criterion for evaluating the frame joint performance is proposed to measure the degree of performance degradation and impact resistance of the joint. The dynamic displacement amplification factor in different temperature environments are proposed for the different beam end constraint types and SDRs. Results of the experimental and numerical analysis show that the welded connection(WC) of three typical joint types is the strongest, and the extended endplate connection(EEC) is the weakest in terms of the impact resistance performance. With regard to the failure mode of the joint, the failure positions of the WC and the welded-bolted connection are located in the inner web of the column. Meanwhile, the EEC is located in the connection position between the beam and the endplate. Three different internal force stages and two obvious critical temperature boundaries of the restrained beams emerge with the increase in temperature, and they have significant characteristics in terms of deformation trend, internal force transfer and energy distribution. During the impact, a phenomenon known as “compression arch action” develops into “catenary action” with the increase in deflection in the frame beam mechanism.

关键词： Restrained beam Connection Temperature Impact Failure modes Internal force mechanism

高分子量麦谷蛋白亚基基因Glu-D1d共显性分子标记在育种中的应用

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江苏农业学报 2008年第4期24卷 394-399页

作者：刘东涛陈荣振冯国华刘世来王来花李德民张会云王静江苏徐淮地区徐州农业科学研究所小麦研究室江苏徐州221121

报道了小麦高分子量(HWM)麦谷蛋白亚基基因Glu-D1 d(编码1Dx5亚基)的一个新的共显性PCR分子标记。该标记由3条引物Dx-F,Dx5-F和Dx-R组成,能够特异地扩增出Glu-D1 d基因及其等位基因的特征条带。结合高分子量麦谷蛋白电泳分析,用16个小... 详细信息

报道了小麦高分子量(HWM)麦谷蛋白亚基基因Glu-D1 d(编码1Dx5亚基)的一个新的共显性PCR分子标记。该标记由3条引物Dx-F,Dx5-F和Dx-R组成,能够特异地扩增出Glu-D1 d基因及其等位基因的特征条带。结合高分子量麦谷蛋白电泳分析,用16个小麦品种对其进行验证,结果表明,该标记的检测结果与蛋白电泳结果完全一致,并能很好地鉴定出杂合基因型。同时将该标记成功地应用于组合周麦18×烟农19 F2分离群体的单株选择上,即在随机选取的88个籽粒(单株)中分别检测到21株Glu-D1 d基因纯合、25株Glu-D1 a基因纯合和42株基因杂合的单株。高分子量麦谷蛋白电泳结果表明,所检测的38个F2单株与PCR检测结果完全一致。该标记是迄今为止最适合优质麦谷蛋白亚基5+10分子标记辅助选择的标记。

关键词：小麦高分子量麦谷蛋白亚基(HMW—GS) PCR 共显性分子标记

黄淮麦区小麦品种高分子量谷蛋白亚基组成分析(英文)

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麦类作物学报 2007年第4期27卷 643-648页

作者：刘东涛冯国华谢佩松陈荣振刘世来王来花张会云李德民王静江苏省徐州农科院江苏徐州221121 扬州大学生物科学与技术学院江苏扬州225009

对黄淮麦区育成推广品种（59个）、2003～2004年度国家黄淮南片和江苏省区试参试品种（42个）、徐州农科所育成的高代品系以及一些育种亲本材料，共计309个品种（材料）的高分子量谷蛋白亚基组成进行了分析。结果共发现了32个亚基组成... 详细信息

对黄淮麦区育成推广品种（59个）、2003～2004年度国家黄淮南片和江苏省区试参试品种（42个）、徐州农科所育成的高代品系以及一些育种亲本材料，共计309个品种（材料）的高分子量谷蛋白亚基组成进行了分析。结果共发现了32个亚基组成类型和16个等位基因变异。在Glu-Al位点发现了Glu-Ala、Glu-Alb、Glu-Alc3个等位基因；在Glu-B1位点发现了Glu-Bla、Glu-Blb、Glu-Blc、Glu-Bld、Glu-Ble、Glu-B1f、Glu-Blg、Glu-B1^、Glu-B1i、Glu-B1女共10个等位基因；在Glu-D1位点发现了Glu-D1a、Glu-D16、Glu-D1d3个等位基因。以Glu-B1位点的变异最为丰富。在这3个位点上分别以等位基因Glu-Alc（null）、Glu-Blb（7＋8）和Glu-Dla（2＋12）为主，其出现频率分别为58．58％、58．90％和77．99％。高分子量谷蛋白亚基组成以（null，7＋8，2＋12）和（1，7＋8，2＋12）为主，分别占所有品种的32．69％和16．18％。在育成推广品种和参试品种中，等位基因变异均为11个；而亚基组成类型则分别为16个和13个。优质高分子量谷蛋白亚基5＋10在所有材料、59个已审定推广品种和42个参试品种中的出现频率分别为20．4％、27．1％％和21．4％，频率均较低。这表明新近育成的品种在优质亚基的构成上并未取得较大进展，优质强筋小麦的品质育种还有较大的发展空间。试验结果也表明，黄淮冬麦区小麦品种的高分子量谷蛋白亚基组成类型和等位基因变异较为丰富，但其变异分布很不均匀，存在明显的优势亚基和组成类型。

关键词：小麦品种黄淮冬麦区高分子量谷蛋白亚基多样性

Location and Trajectory Identification from Microblogs

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Journal of Computer Science & Technology 2019年第4期34卷 727-746页

作者： Na Ta guo-Liang Li Jun Hu Jian-hua feng School of Journalism and Communication Renmin University of ChinaBeijing 100872China Department of Computer Science and Technology Tsinghua UniversityBeijing 100084China Airbnb Network(Beijing)Inc. Beijing 100020China

The rapid development of social networks has resulted in a proliferation of user-generated content(UGC),which can benefit many *** this paper,we study the problem of identifying a user's locations from microblogs,to facilitate effective location-based advertisement and *** the location information in a microblog is incomplete,we cannot get an accurate location from a local *** such,we propose a global location identification method,*** combines multiple microblogs of a user and utilizes them to identify the user's *** not only improves the quality of identifying a user's location but also supplements the location of a microblog so as to obtain an accurate location of a *** facilitate location identification,Glitter organizes points of interest(POIs)into a tree structure where leaf nodes are POIs and non-leaf nodes are segments of POIs,e.g.,countries,cities,and *** the tree structure,Glitter first extracts candidate locations from each microblog of a user which correspond to some tree *** Glitter aggregates these candidate locations and identifies top-κlocations of the *** the identified top-κuser locations,Glitter refines the candidate locations and computes top-κlocations of each *** achieve high recall,we enable fuzzy matching between locations and *** propose an incremental algorithm to support dynamic updates of *** also study how to identify users'trajectories based on the extracted *** propose an effective algorithm to extract high-quality *** results on real-world datasets show that our method achieves high quality and good performance,and scales well.

关键词： location identification microblog trajectory identification

Evidence of Dichogamy in Santalum album L.

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Journal of Integrative Plant Biology 2006年第3期48卷 300-306页

作者： guo-hua Ma Eric Bunn Jing-feng Zhang guo-Jiang Wu South China Botanical Garden the Chinese Academy of Sciences Guangzhou 510650 China Kings Park and Botanic Garden West Perth Western Australia 6005 Australia

Flowering, fruit set, embryological development, and pollination trials were Investigated In Santslum album L. Each ovary may have three to four ovules. Mlcrosporogenesls and megasporogenesls In the same flower were synchronized at the earlier stages of flower development. However, at anthesls, when pollen was mature, the magaspore had developed only to the stage of a one- to two-nucleus embryo sac. As the eight-nucleus embryo sac developed, some mamelon cells began to undergo programmed cell death, forming holes Into which the eight-nucleus embryo sacs extended, becoming ＂N＂ or ＂S＂ shaped. The development from a two-nucleus embryo sac to a matured eight-nucleus embryo sac lasted up to 10 d. Fruit-set from open pollination was less than 2%. The endosperm develops prior to division of the zygotic embryo and one to three embryos and endosperms were formed In the same fruit. A mature seed usually germinates to produce one seedling; however, two and three seedlings from one seed were also observed, albeit at a low frequency. Pollination trials showed that no seed sets when Inflorescences were covered with s bag; however, artificial pollination could Improve fruit set. Our pollinaUon trials and embryological studies proved that the flower of S. album Is dlchogamous and fruit set has high heterozygosity.

关键词： dichogamy embryonic development pollination trials Santalum album.