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Genome Editing in the Olive Flounder(Paralichthys olivaceus)Using CRISPR/Cas9 and a Simple Microinjection System

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Journal of Ocean University of China 2021年第6期20卷 1528-1536页

作者： TAN Xungang WANG Ling WU Zhihao JIAO Shuang WANG Lijuan ZOU Yuxia JIANG Jingteng you feng CAS and Shandong Province Key Laboratory of Experimental Marine Biology Center for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China University of Chinese Academy of Sciences Beijing 100049China Weihai Fishery Technology Promotion Station Weihai 264200China Shenghang Aquatic Science and Technology Co. Ltd.Weihai 264200China

The whole-genome sequence of the olive flounder(Paralichthys olivaceus)provides a basis for gene functional analyses,which is important for the aquaculture *** gene function will help us to select better economic traits such as fast growth and better culture conditions,which further will increase the aquaculture *** knockout is an important reverse genetics approach for in vivo studies of gene *** this study,the CRISPR/Cas9 genome editing method with a microinjection system using a simple braked needle was employed in olive *** injection in embryos,green fluorescent protein expression was detected in 40%of *** proportion of normal-hatched larvae was approximately 50%.Different mutations,including short indels and fragment deletions,were found in our test genes gsdf and ***,we detected more than one mutation in a single *** summary,our microinjection technique and CRISPR/Cas9 can be applied to study gene functions in olive flounder.

关键词： CRISPR/Cas9 gsdf microinjection myomaker needle with brake olive flounder

Recent Progress in Fabrication and Structural Design of Thermal Conductive Polymer Composites

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Chinese Journal of Polymer Science 2024年第3期42卷 277-291,I0004页

作者： Yuan-Kai Du Zheng-Xue Shi Shan Dong Hui Jin Xue Ke Pei Zhao Bing-Bing Jiang feng you Hubei Key Laboratory of Plasma Chemistry and Advanced Materials School of Material Science and EngineeringWuhan Institute of TechnologyWuhan 430205China Key Laboratory of Green Preparation and Application of Functional Materials Ministry of EducationHubei UniversityWuhan 430062China Institute of Molecular Aggregation Science Tianjin UniversityTianjin 300072China

In recent years, the demand direction for electronic equipment has expanded into embedded and miniaturized devices. The heat radiation problem has become one of the most significant factors for hindering the development of electronic devices. Since heat radiation material is one of the important components in electronic devices, the demand for enhancing thermal conductivity is also increasingly urgent. Research on thermal conductive polymer composites has become a major direction for developing functional composites. This work reviewed the recent progress in the fabrication of thermal conductive polymer composites. Five different structures are presented, including the using of single fillers,hybrid fillers, double threshold percolation structure, segregated structure and other complex multiphase structures. Specifically, the preparation of high-performance thermal conductive polymer composites was introduced through the combination of various thermal conductive ***, the development direction of high thermal conductive polymer composites was briefly explored.

关键词： Thermal conductive polymer Hybrid fillers Polymer composite Thermal resistance

Freeze substitution Hi-C,a convenient and cost-effective method for capturing the natural 3D chromatin conformation from frozen samples

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Journal of Genetics and Genomics 2021年第3期48卷 237-247页

作者： Wu Zheng Zhaoen Yang Xiaoyang Ge Yijia feng Ye Wang Chengwei Liu Yanan Luan Kun Cai Serhii Vakal feng you Wei Guo Wei Wang Zhenhua feng Fuguang Li Zhengzhou Research Base State Key Laboratory of Cotton BiologyZhengzhou UniversityZhengzhou 450000China State Key Laboratory of Cotton Biology Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyang 455000China Wenzhou Medical University Wenzhou 325027China Structural Bioinformatics Laboratory BiochemistryAbo Akademi UniversityTurku 20520Finland Department of Otorhinolaryngology-Head and Neck Surgery Changhai HospitalSecond Military Medical UniversityShanghai 200000China

Chromatin interactions functionally affect genome architecture and gene regulation,but to date,only fresh samples must be used in High-through chromosome conformation capture(Hi-C)to keep natural chromatin conformation *** requirement has impeded the advancement of 3 D genome research by limiting sample collection and storage options for researchers and severely limiting the number of samples that can be processed in a short ***,we develop a freeze substitution Hi-C(FS-Hi-C)technique that overcomes the need for fresh ***-Hi-C can be used with samples stored in liquid nitrogen(LN2):the water in a vitreous form in the sample cells is replaced with ethanol via automated freeze *** confirming that the FS step preserves the natural chromosome conformation during sample thawing,we tested the performance of FS-Hi-C with Drosophila melanogaster and Gossypium *** allowing the use of frozen samples and confirming that FS-Hi-C delivers robust data for generating contact heat maps and delineating A/B compartments and topologically associating domains,we found that FS-HiC outperforms the in situ Hi-C in terms of library quality,reproducibility,and valid ***,FS-HiC will probably extend the application of 3D genome structure analysis to the vast number of experimental contexts in biological and medical research for which Hi-C methods have been unfeasible to date.

关键词： FS-Hi-C Frozen sample Chromosome conformation Drosophila melanogaster Gossypium hirsutum

基于信息熵重构经验模态分解神经网络的带式输送机故障诊断

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煤矿机械 2025年第1期46卷 181-185页

作者：尤峰郭刚闫涛吴振彬卢海军中煤陕西榆林能源化工有限公司陕西榆林719000 中煤信息技术(北京)有限公司北京100020

提出了一种神经网络模型,用于带式输送机电机轴承的早期故障诊断。该模型采用经验模态分解与信息熵相结合的方法对信号进行重构,提取频域特征用于神经网络的训练,以实现高度精准的故障诊断。该方法能够有效应对噪声干扰,对早期故障信号... 详细信息

提出了一种神经网络模型,用于带式输送机电机轴承的早期故障诊断。该模型采用经验模态分解与信息熵相结合的方法对信号进行重构,提取频域特征用于神经网络的训练,以实现高度精准的故障诊断。该方法能够有效应对噪声干扰,对早期故障信号具有良好的敏感性,其诊断准确率高达95.8%。

关键词：带式输送机经验模态分解神经网络轴承故障诊断

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断陷盆地层序格架中岩性地层油气藏分布特征

石油学报 2005年第4期26卷 17-22页

作者：冯有良中国石油勘探开发研究院北京100083

运用层序地层学原理,对渤海湾盆地东营、沾化、南堡和西部凹陷的岩性及地层油气藏在层序地层格架中的分布特征进行了分析。结果表明,层序界面控制了地层油气藏的发育,主裂陷幕三级层序湖扩展域优质烃源岩控制岩性油气藏发育的层段,盆缘... 详细信息

运用层序地层学原理,对渤海湾盆地东营、沾化、南堡和西部凹陷的岩性及地层油气藏在层序地层格架中的分布特征进行了分析。结果表明,层序界面控制了地层油气藏的发育,主裂陷幕三级层序湖扩展域优质烃源岩控制岩性油气藏发育的层段,盆缘沟谷和构造坡折带控制的层序低位域和高位域砂体是岩性油藏的主要储集体,该砂体发育带与湖扩展域优质烃源岩的有效配置部位就是岩性油气藏的富集带。高异常流体压力封存箱保留了原生孔隙,有机酸产生了次生孔隙,流体压裂、幕式排烃以及高异常流体压力有利于克服储层毛细管阻力,这些特性使得有高异常流体压力封存箱发育的受坡折带控制的岩性油气藏富集、高产。

关键词：层序地层学断陷盆地压力封存箱同沉积构造坡折带地层油气藏岩性油气藏

Sap Flow of Populus euphratica in a Desert Riparian Forest in an Extreme Arid Region During the Growing Season

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Journal of Integrative Plant Biology 2007年第4期49卷 425-436页

作者： Jian-Hua Si Qi feng Xiao-you Zhang Zong-Qiang Chang Yong-Hong Su Hai-Yang Xi Cold and Arid Region Environmental and Engineering Research Institute the Chinese Academy of Sciences Alashan Desert Eco-hydrology Experimental Research Station Lanzhou 730000 China

In the present study, the heat pulse technique was applied to investigate the stem sap flow of Populus euphratica in a desert riparian forest in an extreme arid region from April to October 2003 and from May to October 2004. The experimental sites were in Qidaoqiao （101 °10′ E, 41°59′ N） and Bayantaolai farm （101°14′ E, 42°01′ N） in Ejina county, in the low reaches of the Heihe River, China. The results indicated that the diurnal change in the velocity of sap flow showed minor fluctuations. At night, the rising of sap flow could be observed in the main tree species because of root pressure. During the growing season, the maximum average velocity was observed in July, followed by August, and the same velocity was observed in September and May; the minimum velocity was observed in October. The transpiration from June to August during the growing season accounted for approximately 70% of the annual total transpiration. The sap flow velocity of P. euphratica trees of different ages could be arranged in the order： 15 yr 〉 25 yr 〉 50 yr. Sap flow velocity was closely related to changes in micrometeorological factors, with average sap flow velocity showing a significant linear correlation with net radiation, air temperature and relative humidity.

关键词： desert riparian forest extreme arid region heat-pulse technique Populus euphratica sap flow velocity.

Influencing Factors and Product Toxicity of Anthracene Oxidation by Fungal Laccase

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Pedosphere 2014年第3期24卷 359-366页

作者： LI Xuan-Zhen CHENG Qian WU Yu-Cheng feng you-Zhi LIU Wei-Wei LIN Xian-Gui Key Laboratory of Soil Environment and Pollution Remediation Institute of Soil Science Chinese Academy of Sciences State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences Technology Center of China Tobacco Hebei Industrial Co. Ltd.

The transformation profiles of polycyclic aromatic hydrocarbons （PAHs） by pure laccases from Trametes versicolor and Pycnoporus sanguineus, and the optimal reaction conditions （acetonitrile concentration, pH, temperature and incubation time） were determined. Anthracene was the most transformable PAH by both laccases, followed by benzo[a]pyrene, and benzo[a]anthracene. Laccase-mediator system （LMS） could not only improve the PAH oxidation but also extend the substrate types compared to laccase alone. 5e/0 or 10~ （v/v） of acetonitrile concentration, pH 4, temperature of 40 ~C, and incubation time of 24 h were most favorable for anthracene oxidation by laccase from T. versicolor or P. sanguineus. The gas chromatography-mass spectrometry analysis indicated that 9,10- anthraquinone was the main product of anthracene transformed by laccase from T. versicolor. Microtox test results showed that both anthracene and its laccase-transformation products were not acute toxic compounds, suggesting that laccase-treatment of anthracene would not increase the acute toxicity of contaminated site.

关键词： laccase-mediator system polycyclic aromatic hydrocarbons Pycnoporus sanguineus reaction condition Trametes versicolor

Association of 370-371insACA, 494T〉C, and 1423C〉T haplotype in ubiquitin-specific protease 26 gene and male infertility： a meta-analysis

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Asian Journal of Andrology 2014年第5期16卷 720-724,I0008页

作者： Jia-Dong Xia Jie Chen you-feng Han Hai Chen Wen Yu Yun Chen Yu-Tian Dai Department of Andrology Nanjing Drum Tower Hospital Nanjing Medical University Department of Urology The First Affiliated Hospital of Nanjing Medical University Department of Obstetrics and Gynecology Nanjing Drum Tower Hospital Nanjing Medical University Nanjing China

Whether the 370-371insACA, 494T〉C, and 1423C〉T haplotype in ubiquitin-specific protease 26 （USP26） gene is associated with male infertility is controversial. To clarify this issue, we conducted a meta-analysis based on the most recent studies. Eligible studies were screened by using PubMed and Embase. Pooled odd ratio （OR） with 95% confidence interval （CI） was calculated with fixed effect models. Ten studies with 1603 patients and 2505 controls were included, Overall, the results indicated that there was an association between the haplotype and male infertile risk （OR = 1.74, 95% CI： 1.09-2.77）. The OR calculated based on the five studies in Asia and three in Europe was 1.96 （95% CI： 1,05-3.67） and 1.54 （95% Ch 0.75-3.16） respectively, however, the OR was 0.86 （95% Ch 0.05-15,29） based on the two investigations in America. In addition, the data from the patients with azoospermia （AZO） showed an increased pooled OR of 2.35 （95% Cl： 1.22-4.50）. In contrast, the studies with oligoasthenoteratozoospermia （OAT） exhibited that the pooled OR was 0,97 （95% Ch 0.43-2.16）. Our analyses indicate that there is an association of alteration in USP26 with male infertility, especially in AZO and Asian population.

关键词： haplotype male infertility meta-analysis ubiquitin-specific protease 26

Comparison of surgical effect and postoperative patient experience between laparoendoscopic single-site and conventional laparoscopic varicocelectomy, a *** and meta-analysis

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Asian Journal of Andrology 2017年第2期19卷 248-255页

作者： Zheng Zhang Shu-Juan Zheng Wen Yu you-feng Han Hai Chen Yun Chen Yu-Tian Dai Department of Andrology Affiliated Drum Tower Hospital School of Medicine Nanjing University Nanjing 210008 China

The present meta-analysis was conducted to compare the clinical effect and patient experience of laparoendoscopic single-site varicocelectomy （LESSV） and conventional laparoscopic varicocelectomy. The candidate studies were included after literature search of database Cochrane Library, PubMed, EMBASE, and MEDLINE. Related information on essential data and outcome measures was extracted from the eligible studies by two independent authors, and a meta-analysis was conducted using STATA 12.0 software. Subgroup analyses were conducted by study design （RCT and non-RCT）. The odds ratio （OR） or standardized mean difference （SMD） and their 95% confidence intervals （95% CIs） were used to estimate the outcome measures. Seven articles were included in our meta-analysis. The results indicated that patient who had undergone LESSV had a shorter duration of back to work （overall： SMD = -1.454, 95% Ch -2.502m0.405, P= 0.007; non-RCT： SMD = -2.906, 95% Ch -3.796-2.017, P= 0.000; and RCT： SMD = -0.841, 95% Ch -1.393-0.289, P = 0.003） and less pain experience at 3 h or 6 h （SMD = -0.447, 95% Ch -0.754-0.139, P = 0.004）, day 1 （SMD = -0.477, 95% Ch -0.905-0.05, P = 0.029）, and day 2 （SMD = -0.612, 95% Ch -1.099-0.125, P= 0.014） postoperatively based on RCT studies. However, the meta-analyses based on operation time, clinical effect （improvement of semen quality and scrotal pain relief）, and complications （hydrocele and recurrence） yielded nonsignificant results. In conclusion, LESSV had a rapid recovery and less pain experience over conventional laparoscopic varicocelectomy. However, there was no statistically significant difference between the two varicocelectomy techniques in terms of the clinical effect and the incidence of hydrocele and varicocele recurrence. More high-quality studies are warranted for a comprehensive conclusion.

关键词： complications conventional laparoscopic varicocelectomy laparoendoscopic single-site varicocelectomy meta-analysis pain score semen quality